FlyBase Allele Report: Dmel\numb[1]

In mutant embryos the number of myocardial cells in segments A2 to A7 is reduced from six to four per hemisegment. The reduction of myocardial cells is paralleled by an increase in eve expressing pericardial cells from two to four in 80% of hemisegments, accompanied by a loss of DA1 and DO2 muscles.

(Han and Bodmer, 2003)

The development of the EW2 and EW3 neurons of the NB7-3 lineage is dramatically altered in numb¹ mutants, but there is only a mild effect on EW1 neuron development. Novel apoptosis within the NB7-3 lineage is seen in the mutant embryos. The identity of the A8 serotonergic neuron in the central nervous system has a change of identity in mutant embryos, having expression characteristics similar to EW1 cells (rather than characteristics similar to EW2 cells, as occurs in wild-type embryos).

(Lundell et al., 2003)

92% of heterozygous flies show rhythmic locomotor activity, with a period of 24.4 +/- 0.1 hours. 93% of numb¹/numb^SW flies show rhythmic locomotor activity, with a period of 24.6 +/- 0.1 hours.

(Stempfl et al., 2002)

The dorsal bipolar dendritic (dbd) and dorsal dendritic arbor (dda) neurons are absent in mutant embryos.

(Umesono et al., 2002)

In mutant embryos a loss of cardioblasts is seen. An increase in the number of eve expressing pericardial cells is seen.

(Gajewski et al., 2000)

In mutant embryos Malpighian tubule tip cells are missing except in about 8% embryos, where one or two of the four tubules are allocated normally.

(Wan et al., 2000)

In mutant embryos the DO5^A muscle is duplicated at the expense of DA3^A in most segments.

(Crozatier and Vincent, 1999)

11% of hemisegments show an RP2 to RP2sib cell fate transformation in numb¹/numb⁷⁹⁶ embryos. 23% of hemisegments show an RP2 to RP2sib cell fate transformation in numb¹/Df(2L)30A-C embryos. numb¹ insc²² double mutant embryos lack an eve-positive cell in the RP2 position in 98% of hemisegments.

(Buescher et al., 1998)

Muscles DA1 and DO1 are sometimes missing in homozygous embryos. The number of eve-expressing pericardial cells is increased compared to wild-type.

(Carmena et al., 1998)

Stage 15 numb¹ homozygous embryos have 3-8 neurons per hemisegment (compared to approximately 35 in wild-type).

(Dye et al., 1998)

Homozygous embryos have supernumerary lateral adult muscle precursors and lack segmental border muscles.

(Jagla et al., 1998)

Many muscles are absent in numb¹/numb² embryos, particularly in the dorsal region. Most myocardial cells are formed. The number of eve-expressing pericardial cells is double that of wild-type.

(Park et al., 1998)

The RP2sib neuron, vMP2 neuron and U neuron are all duplicated at the expense of their siblings. The number of EL neurons is strongly reduced. pCC/aCC are unaffected. This phenotype is the reciprocal of that shown for spdo, Dl, N and mam embryos. The phenotype of numb; spdo double mutants is identical to embryos lacking spdo alone. The eve⁺ EL neurons are completely rescued.

(Skeath and Doe, 1998)

Homozygous embryos have severe defects in the muscle pattern, including the loss of the dorsal set of muscles (including DA1) and the loss of muscles LL1 and VA3. Transformations of muscles LT4 towards LT3, and VA2 to VA1 are seen. Duplication of muscle DO5 is seen. Each of the six persistent twi-expressing adult muscle precursors in an abdominal hemisegment is duplicated. The duplication of the lateral adult precursors is associated with a loss of the segment border muscle, while the duplication of the three dorsal precursors is associated with the loss of dorsal muscles. Embryos have an excess of pericardial cells.

(Ruiz Gomez and Bate, 1997)

Embryos exhibit an absence of dorsal md neurons, md neurons, they either do not form, or lose their ability to express ct, or die.

(Vervoort et al., 1997)

Severe loss of neurons in the peripheral nervous system, the IIb cell adopts the fate of its sister IIa cell.

(Zhong et al., 1997)

Homozygous embryos exhibit less than 10% of neurons than that of wild type.

(Guo et al., 1996)

dMP2 is transformed to the vMP2 fate. In numb¹ Dl³ and numb¹ Df(1)N-81k1 double mutants all MP2 neurons develop as dMP2.

(Spana and Doe, 1996)

Severe neuronal loss caused by transformation of both neurons and sheath cells into outer support cells.

(Zhong et al., 1996)

Sensory organ related md neurons are usually absent or transformed into support cells.

(Brewster and Bodmer, 1995)

In every segment dMP2 is transformed into a vMP2 neuron that lacks odd expression and has an anterior axon projection. The physical asymmetry of the MP2 division is not altered, only the fate of the daughter cells.

(Spana et al., 1995)

Disrupts differentiation of peripheral sensory neurons and deletion of several ventral longitudinal muscle fibres leaving one (muscle 6) or occasionally two in the embryo. Organisation of the CNS neuropil is unaltered. RP3 innervates muscle 6 in most embryos but can occasionally fail to synapse. RP1 fails to synapse with the only longitudinal fibre, instead continues to grow distally.

(Chiba et al., 1993)

Homozygous embryos lack most of the sensory neurons of the peripheral nervous system, including the lateral nerve cell clusters of the abdominal segments.

(Kovalick and Beckingham, 1992)

Number of peripheral sensory neurons in a thoracic or abdominal hemisegment is severely reduced. The es neurons and glial cells are transformed into support cells, ch organ morphology is not maintained and md neurons are removed. One pleural external transverse muscle is missing and the oblique muscles that transverse each segment have abnormal arrangements.

(Uemura et al., 1989)

apparently null since the neuronal phenotype of homozygotes is indistinguishable from that of hemizygotes.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

numb¹ has abnormal neuroanatomy | recessive | embryonic stage phenotype, suppressible | partially by Df(3R)Espl-X10/Df(3R)Espl-X10

(Mujizah et al., 2024)

numb¹ has abnormal neuroanatomy phenotype, suppressible by spdo^G104/spdo[+]

(Lundell et al., 2003)

numb¹ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^eg-Mz360/BacA\p35^UAS.cHa

(Lundell et al., 2003)

Enhancer of

Statement

Reference

numb[+]/numb¹ is an enhancer of visible phenotype of Nak^UAS.cCa, Scer\GAL4^sca-537.4

(Chien et al., 1998)

Other

Statement

Reference

numb[+]/numb¹, wts^x1 has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

numb¹ has PNS neuron | embryonic stage phenotype, suppressible | partially by Df(3R)Espl-X10/Df(3R)Espl-X10

(Mujizah et al., 2024)

numb¹ has EW2 neuron phenotype, suppressible by spdo^G104/spdo[+]

(Lundell et al., 2003)

numb¹ has EW3 neuron phenotype, suppressible by spdo^G104/spdo[+]

(Lundell et al., 2003)

numb¹ has embryonic peripheral nervous system & neuron phenotype, suppressible by spdo^H7

(Dye et al., 1998)

numb¹ has abdominal dorsal bipolar neuron dbp | embryonic stage phenotype, suppressible by spdo^H7

(Dye et al., 1998)

numb¹ has embryonic peripheral nervous system & glial cell | supernumerary phenotype, suppressible by spdo^H7

(Dye et al., 1998)

numb¹ has neuron phenotype, suppressible by Mmus\Numbl^UAS.Tag:MYC/Scer\GAL4^h-1J3

(Zhong et al., 1997)

numb¹ has phenotype, suppressible by N^l1N-ts1

(Guo et al., 1996)

numb¹ has neuron phenotype, suppressible by Scer\GAL4^h-1J3/Mmus\Numb^UAS.Tag:MYC

(Zhong et al., 1996)

Enhancer of

Statement

Reference

numb[+]/numb¹ is an enhancer of wing sensillum phenotype of Nak^UAS.cCa, Scer\GAL4^sca-537.4

(Chien et al., 1998)

numb[+]/numb¹ is an enhancer of head sensillum phenotype of Nak^UAS.cCa, Scer\GAL4^sca-537.4

(Chien et al., 1998)

numb[+]/numb¹ is an enhancer of adult mesothoracic sensillum phenotype of Nak^UAS.cCa, Scer\GAL4^sca-537.4

(Chien et al., 1998)

Suppressor of

Statement

Reference

numb[+]/numb¹ is a suppressor | partially of scutellar bristle | ectopic phenotype of Scer\GAL4^ptc-559.1, spdo^UAS.cOa

(Babaoglan et al., 2009)

Other

Statement

Reference

numb[+]/numb¹, wts^x1 has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

CycA^C8LR1, numb¹ has pericardial cell phenotype

(Han and Bodmer, 2003)

CycA^C8LR1, numb¹ has muscle cell of A1-7 dorsal acute muscle 1 phenotype

(Han and Bodmer, 2003)

CycA^C8LR1, numb¹ has muscle cell of mesothoracic dorsal acute muscle 1 phenotype

(Han and Bodmer, 2003)

CycA^C8LR1, numb¹ has muscle cell of metathoracic dorsal acute muscle 1 phenotype

(Han and Bodmer, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in wts^x1/+;numb¹/+ double heterozygous embryos compared to either of the single heterozygotes or wild type.

(Keder et al., 2015)

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a numb¹/+ background.

(Babaoglan et al., 2009)

In numb¹, CycA^C8LR1 double mutant embryos the formation of one non-myogenic eve expressing cell per segment is seen, apparently at the expense of DA1 muscle formation.

(Han and Bodmer, 2003)

spdo^G104/+ partially rescues formation of the EW2 and EW3 neurons in numb¹ embryos.

(Lundell et al., 2003)

Dominantly enhances the adult external sensory organ phenotype produced by Nak^Scer\UAS.cCa expressed under the control of Scer\GAL4^sca-537.4.

(Chien et al., 1998)

spdo^H7/spdo^H7 largely suppresses the reduction in neuron numbers seen in stage 15 numb¹ homozygous embryos. The phenotype of double mutants with spdo^H7 does not resemble the numb¹ phenotype, but the spdo mutant phenotype. In the absence of numb two dbd glial cells are seen. In double mutants with spdo^H7, the dbd glial cell is missing, as for spdo mutants.

(Dye et al., 1998)

Embryos homozygous for numb¹ and N^l1N-ts1 at the nonpermissive temperature exhibit neuronal overproduction.

(Guo et al., 1996)

numb¹; ttk^osn double mutant overproduces neurons, indistinguishable from ttk^osn mutant.

(Guo et al., 1995)

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^h-1J3-mediated expression of Mmus\Numbl^Scer\UAS.cZa rescues the neuronal loss phenotype.

(Zhong et al., 1997)

Scer\GAL4^h-1J3 induced expression of Mmus\Numb^Scer\UAS.cZa rescues the neuronal phenotype of numb¹ embryos and rescues the two dorsal most es organs in abdominal hemisegments to wild type.

(Zhong et al., 1996)

Complementation and Rescue Data

Fails to complement

numb^nuts

(Stempfl et al., 2002)

Rescued by

numb¹ is rescued by numb^{fTRG00025.sfGFP-TVPTBF}

(Mujizah et al., 2024)

numb⁷⁹⁶/numb¹ is rescued by numb^+t19.5

(Rebeiz et al., 2011)

Partially rescued by

numb⁷⁹⁶/numb¹ is partially rescued by numb^2del

(Rebeiz et al., 2011)

Comments

numb^+t19.5 efficiently rescues the lethality associated with numb¹/numb⁷⁹⁶. Adult mechanosensory organ development is almost completely normal in the rescued flies.

numb¹/numb⁷⁹⁶ flies rescued by numb^2del show a widespread "double socket" phenotype, in which the shaft cell is transformed to a second socket cell.

(Rebeiz et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

4096

107585

Notes on Origin

Discoverer

Stock contains a second-site deletion uncovering l(2)gl.

(Roegiers et al., 2009)

Comments

On the basis of CNS phenotype numb alleles form a series: numb² > numb⁴ > numb¹/numb³.

(Skeath and Doe, 1998)

P-element reversion demonstrates the P-element insertion is responsible for the mutant phenotype.

(Uemura et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

AS125

(Ricard et al., 2001)

l(2)AS125

(Lane and Kalderon, 1993)

l(2)neo7

(Beaton, 1999.12.12)

nb1

(Shen et al., 1997)

nb¹

(Weavers and Skaer, 2013, Hardiman et al., 2002, Wan et al., 2000, Crozatier and Vincent, 1999, Buescher et al., 1998, Carmena et al., 1998, Jagla et al., 1998, Skeath and Doe, 1998)

numb1

(Zhong et al., 1996)

numbⁿ⁷

(Brewster and Bodmer, 1995)

Name Synonyms

Secondary FlyBase IDs

References (64)

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