FlyBase Allele Report: Dmel\upd1[YM55]

General Information

Symbol

Dmel\upd1^YM55

Species

D. melanogaster

Name

FlyBase ID

FBal0013297

Feature type

allele

Associated gene

Dmel\upd1

Associated Insertion(s)

Carried in Construct

Also Known As

upd^YM55, upd⁴

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Agaisse et al., 2003)

Mutagen

ethyl methanesulfonate

(Eberl et al., 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q55term.

(Hombria et al., 2005)

Nucleotide substitution: C?T.

(Sefton et al., 2000, Harrison et al., 1998)

Amino acid replacement: ?60term.

(Sefton et al., 2000)

Amino acid replacement: ??term.

(Harrison et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:18,307,422..18,307,422 [-]

Nucleotide change:

C18307422T

Reported nucleotide change:

C?T

Amino acid change:

Q60term | upd1-PA; Q60term | upd1-PB; Q60term | upd1-PC

Reported amino acid change:

Q60term

(Sefton et al., 2000, Harrison et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal axis specification | embryonic stage

(Lai et al., 2023)

abnormal cell shape | extended germ band stage

(Bertet et al., 2009)

abnormal planar polarity | recessive | somatic clone

(Zeidler et al., 1999)

abnormal size | adult stage, with upd1^GMR.PB

(Bach et al., 2003)

lethal

(Wang et al., 2014)

lethal (with Df(1)os1A2)

(Wang et al., 2014)

lethal | recessive

(Sefton et al., 2000, Eberl et al., 1992)

lethal - all die during embryonic stage | recessive

(Perrimon et al., 1989)

some die during embryonic stage | recessive

(Perrimon et al., 1989)

visible

(Hombria et al., 2005)

visible (with Df(1)os¹)

(Wang et al., 2014)

visible (with Df(1)os^o)

(Wang et al., 2014)

visible | recessive

(Eberl et al., 1992)

Phenotype Manifest In

border follicle cell

(Silver and Montell, 2001)

border follicle cell (with upd1^sisC-5)

(Xi et al., 2003)

border follicle cell | somatic clone

(Grammont and Irvine, 2002)

ectoderm | extended germ band stage

(Bertet et al., 2009)

embryonic/larval gut | embryonic stage

(Lai et al., 2023)

embryonic abdominal segment 4

(Hombria et al., 2005)

embryonic abdominal segment 5

(Hombria et al., 2005)

eye | somatic clone

(Zeidler et al., 1999)

gonad | ectopic & embryo

(Li et al., 2003)

ommatidium | somatic clone

(Zeidler et al., 1999)

wing (with Df(1)os¹)

(Wang et al., 2014)

wing (with Df(1)os^o)

(Wang et al., 2014)

Detailed Description

Statement

Reference

upd1^YM55/Df(1)os¹ mutant flies have outstretched wings. Eye size is similar to wild type.

upd1^YM55/Df(1)os^o mutant flies have outstretched wings. Eye size is similar to wild type.

(Wang et al., 2014)

25% of upd1^YM55 homozygous mutant embryos show defects in germ-band extension. Germ-band epithelial cells are normal in the head and dorsal-most regions of the embryo, however intercalating (ventrolateral) cells show a smaller apical cell surface. Germ-band cell intercalation is disrupted or, in extreme cases, blocked.

(Bertet et al., 2009)

upd1^YM55 embryos show a segmentation defect in which the A4 and A5 segments are fused.

(Hombria et al., 2005)

The enlarged eye phenotype seen in flies carrying one copy of upd1^GMR.PB is not modified if the flies are also carrying upd1^YM55.

(Bach et al., 2003)

The pole cells of upd1^YM55 mutant embryos are able to migrate normally and coalesce to form a pair of gonads in late embryos. However, one gonad often becomes located one segment offset from its proper location.

(Li et al., 2003)

Migration of prospective border follicle cells in upd1^sisC-5/upd1^YM55 animals is delayed or defective. Marker analysis suggests that these cells are at least partially transformed to a nurse follicle cell fate.

(Xi et al., 2003)

Mosaic stage 9-10A follicles that have upd1[-] anterior polar cells contain fewer than normal border cells and the border cells fail to migrate. Morphologically normal stretched and centripetal cells are present in these follicles.

Mosaic stage 9-10 follicles that have upd1[-] posterior polar cells have a normally localised oocyte at the posterior part of the follicle and the oocyte nucleus is correctly positioned.

(Grammont and Irvine, 2002)

When homozygous somatic clones are made in the egg chamber, defects in border cell migration are seen. Whenever the polar cells are mutant, a complete failure in border cell migration is seen. However, in egg chambers in which the germ-line is mutant, no migration defect is seen. In mutant egg chambers the outer border cell clusters consist of an average of 2.2 cells, as compared to 6 in wild-type.

(Silver and Montell, 2001)

Homozygous clones within the eye field show no phenotype either in imaginal discs or in the adult eye. Clones adjacent to the optic stalk produce almost complete dorsalisation of the eye field as assayed by ommatidial polarity.

(Zeidler et al., 1999)

Defect in the fourth and fifth abdominal denticle belt. This does not produce embryonic lethality as mutant larvae, pupae and sometimes adult can develop lacking the abdominal region. T2, T3 and A8 are sometimes deleted, the head is usually involuted but most head structures are present. Germline clone analysis of os alleles of the upd complementation group indicate they are not required for germline viabilty and do not have a maternal effect. Zygotic lethality acts in the embryo, larval and pupal stages.

(Eberl et al., 1992)

Interacts with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

embryonic lethal. Mutants show variable larval cuticular phenotype with defects predominantly in the mesothorax and the fifth abdominal segment (Nusslein-Volhard et al., 1984; Gergen and Wieschaus, 1986); also some head defects and fourth, sixth, seventh and eighth abdominal segment defects. Heterozygotes show 88-93% viability (Wieschaus and Noell, 1986). T2 defect; rudimentary filzkorper and posterior spiracles; A6 and A7 denticle belts fused; A8 denticle belt reduced

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

upd1^YM55 has abnormal cell shape | extended germ band stage phenotype, suppressible by WASp[+]/WASp³

(Bertet et al., 2009)

upd1^YM55 has abnormal cell shape | extended germ band stage phenotype, suppressible by sqh^AX3

(Bertet et al., 2009)

Enhancer of

Statement

Reference

upd1^YM55 is an enhancer of lethal phenotype of hop²⁵/hop²⁹

(Harrison et al., 1998)

upd1^YM55 is an enhancer of lethal phenotype of hop^M637/hop²⁵

(Harrison et al., 1998)

upd1^YM55 is an enhancer of partially lethal phenotype of hop³²/hop²⁵

(Harrison et al., 1998)

upd1^YM55 is an enhancer of partially lethal phenotype of hop³³/hop²⁵

(Harrison et al., 1998)

upd1^YM55 is an enhancer of partially lethal phenotype of hop³/hop²⁵

(Harrison et al., 1998)

NOT Enhancer of

Statement

Reference

upd1[+]/upd1^YM55 is a non-enhancer of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

NOT Suppressor of

Statement

Reference

upd1[+]/upd1^YM55 is a non-suppressor of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

os[+]/upd1^YM55 is a non-suppressor of abnormal neuroanatomy phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Other

Statement

Reference

hop¹², upd1^YM55 has partially lethal | recessive phenotype

(Harrison et al., 1998)

hop¹⁴, upd1^YM55 has partially lethal | recessive phenotype

(Harrison et al., 1998)

hop²⁵, upd1^YM55 has lethal | recessive phenotype

(Harrison et al., 1998)

hop²⁷, upd1^YM55 has partially lethal | recessive phenotype

(Harrison et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

upd1^YM55 has ectoderm | extended germ band stage phenotype, suppressible by sqh^AX3

(Bertet et al., 2009)

upd1^YM55 has ectoderm | extended germ band stage phenotype, suppressible by Rho1^k02107b

(Bertet et al., 2009)

upd1^YM55 has ectoderm | extended germ band stage phenotype, suppressible by WASp[+]/WASp³

(Bertet et al., 2009)

NOT suppressed by

Statement

Reference

upd1^YM55 has phenotype, non-suppressible by eRF1[+]/eRF1^F2

(Chao et al., 2003)

Enhancer of

Statement

Reference

os[+]/upd1^YM55 is an enhancer of egg chamber phenotype of hop²⁷/hop²⁵

(McGregor et al., 2002)

os[+]/upd1^YM55 is an enhancer of polar follicle cell | increased number phenotype of hop²⁷/hop²⁵

(McGregor et al., 2002)

NOT Enhancer of

Statement

Reference

upd1[+]/upd1^YM55 is a non-enhancer of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

NOT Suppressor of

Statement

Reference

upd1[+]/upd1^YM55 is a non-suppressor of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

os[+]/upd1^YM55 is a non-suppressor of eye disc phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

os[+]/upd1^YM55 is a non-suppressor of glial cell phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of egr^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in upd1^YM55/+ background does not lead to ectopic wings compared to controls.

(Worley et al., 2018)

The glial overmigration phenotype seen in spin¹⁰⁴⁰³ eye discs is not suppressed if the animals are also heterozygous for upd1^YM55.

(Yuva-Aydemir et al., 2011)

An average of 4.5% upd1^YM55, sqh^AX3 homozygous mutant embryos show germ-band extension defects, much reduced compared to upd1^YM55 homozygous mutant embryos. Ectopic apical cell constriction defects are rescued in upd1^YM55, sqh^AX3 homozygous mutant embryos.

An average of 6% upd1^YM55, Rho1^k02107b homozygous mutant embryos show germ-band extension defects, much reduced compared to upd1^YM55 homozygous mutant embryos.

The reduced ectodermal apical cell surface size observed in upd1^YM55 mutant embryos at mid germ-band extension is rescued in upd1^YM55; WASp³/+ embryos.

(Bertet et al., 2009)

The pupal lethality of Df(2R)P3C homozygotes is partially suppressed by upd1^YM55/+.

(Classen et al., 2009)

92% of hop²⁵ upd1^YM55/hop²⁷ egg chambers are fused and the number of extra polar cells is increased compared to hop²⁵/hop²⁷ single mutants.

(McGregor et al., 2002)

Dominantly enhances the phenotype of hop²⁵ in transheterozygous combination with hop²⁷, hop¹⁴, hop³, hop¹², hop³³, hop³², hop^M637 or hop²⁹.

(Harrison et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

upd3^d232a

(Wang et al., 2014)

upd3^x21b

(Wang et al., 2014)

Rescued by

upd1^YM55 is rescued by Scer\GAL4^{VP16.mat.αTub67C}/upd1^UAS.cZa

(Bertet et al., 2009)

Comments

Expression of upd1^Scer\UAS.cZa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the germ-band extension and apical cell shape defects observed in upd1^YM55 mutant embryos.

(Bertet et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

4767

107864

Notes on Origin

Discoverer

Comments

Maternal germline clonal analysis demonstrates there is no maternal effect.

(Perrimon et al., 1989)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF231684

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

YM55

(Eberl et al., 1992)

os^upd-4

(Gutierrez-Aviño et al., 2009)

upd1^YM55

(Worley et al., 2018)

upd⁴

(Lai et al., 2023, Yuva-Aydemir et al., 2011, Gutierrez-Aviño et al., 2009, Zeidler et al., 1999)

upd^YM55

(Bertet et al., 2009, Classen et al., 2009, Wang and Harrison, 2007, Hombria et al., 2005, Wawersik et al., 2005, Li et al., 2003, Xi et al., 2003, Grammont and Irvine, 2002, Karsten et al., 2002, McGregor et al., 2002, Silver and Montell, 2001, Harrison et al., 1998, Klingler and Gergen, 1993, Parkhurst and Ish-Horowicz, 1991, Perrimon et al., 1989)

upd^ym55

(Bach et al., 2003)

updYM55

(Agaisse et al., 2003)

upd[YM55]

(Wang et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (28)

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