FlyBase Allele Report: Dmel\osa[2]

General Information

Symbol

Dmel\osa²

Species

D. melanogaster

Name

FlyBase ID

FBal0013299

Feature type

allele

Associated gene

Dmel\osa

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Gindhart and Kaufman, 1995)

Mutagen

ethyl methanesulfonate

(Kennison and Tamkun, 1988)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-5

(Boivin et al., 2003)

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-27

(Boivin et al., 2003)

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-31

(Boivin et al., 2003)

lethal (with osa^Arv1)

(Talbert and Garber, 1994)

lethal | embryonic stage | recessive

(Vazquez et al., 1999)

lethal | embryonic stage | recessive | rescuable maternal effect

(Vazquez et al., 1999)

lethal | recessive

(Kennison and Tamkun, 1988)

non-enhancer of variegation, with In(1)w^m4

(Nakayama et al., 2012)

non-enhancer of variegation, with P{wA}4-4

(Boivin et al., 2003)

non-suppressor of variegation, with In(1)w^m4

(Nakayama et al., 2012)

non-suppressor of variegation, with P{wA}4-4

(Boivin et al., 2003)

Phenotype Manifest In

embryonic/first instar larval cuticle | germline clone

(Vazquez et al., 1999)

embryonic epidermis | germline clone

(Vazquez et al., 1999)

embryonic segment | germline clone

(Vazquez et al., 1999)

Detailed Description

Statement

Reference

osa²/+ does not affect the position effect variegation at the w locus which is seen in In(1)w^m4 flies.

Heterozygous adult males do not show transformation of A6 to A5.

(Nakayama et al., 2012)

osa²/+ enhances the telomeric position effect (TPE) of P{hsp26-pt-T}39C-5, P{hsp26-pt-T}39C-27 and P{hsp26-pt-T}39C-31. osa²/+ has no effect on the telomeric position effect (TPE) of P{wA}4-4.

(Boivin et al., 2003)

osa¹/osa² embryos (lacking zygotic osa function) die late in embryogenesis with no clear defects in segmentation or segment identity. Embryos derived from homozygous female germline clones without paternal rescue (lacking both maternal and zygotic osa function) do not differentiate any cuticle. Embryos derived from homozygous female germline clones with paternal rescue (lacking maternal osa function) secrete cuticle but have severe defects in segmentation, resembling gap gene mutants.

(Vazquez et al., 1999)

Mutation changes the level of w expression in ph-p^lac+3 flies; eye colour is lighter.

(Fauvarque et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

osa² has visible phenotype, enhanceable by corto[+]/corto⁴²⁰

(Lopez et al., 2001)

Enhancer of

Statement

Reference

osa² is an enhancer of visible phenotype of sd¹

(Jin et al., 2013)

osa²/osa[+] is an enhancer of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

osa², osa[+], brm[+], brm² is an enhancer of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

osa²/osa[+] is an enhancer of visible phenotype of corto⁴²⁰

(Lopez et al., 2001)

osa² is an enhancer of visible phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

NOT Enhancer of

Statement

Reference

osa²/osa[+] is a non-enhancer of visible phenotype of ct^53d

(Krupp et al., 2005)

osa²/osa[+] is a non-enhancer of visible phenotype of ct^K

(Krupp et al., 2005)

osa²/osa[+] is a non-enhancer of visible | homeotic phenotype of Dsp1¹

(Decoville et al., 2001)

osa² is a non-enhancer of visible phenotype of BEAF-32^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 2001)

Suppressor of

Statement

Reference

osa² is a suppressor of visible | homeotic phenotype of Antp^Ns

(Ragab et al., 2006)

osa²/osa[+] is a suppressor of increased cell death phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

osa²/osa[+] is a suppressor of visible | dominant | homeotic phenotype of Antp^Ns

(Vazquez et al., 1999)

osa² is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

osa²/osa[+] is a suppressor of visible | homeotic phenotype of Antp^Ns, Pc²

(Kennison and Tamkun, 1988)

NOT Suppressor of

Statement

Reference

osa² is a non-suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

osa²/osa[+] is a non-suppressor of visible phenotype of ct^53d

(Krupp et al., 2005)

osa²/osa[+] is a non-suppressor of visible phenotype of ct^K

(Krupp et al., 2005)

osa² is a non-suppressor of visible phenotype of BEAF-32^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 2001)

Other

Statement

Reference

γTub23C^Pl-2, osa² has visible | dominant phenotype

(Vázquez et al., 2008)

brm^22D, osa² has lethal | male phenotype

(Armstrong et al., 2005)

brm², osa² has visible | dominant phenotype

(Vazquez et al., 1999)

Ras85D^V12.sev, osa², sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

osa² has wing phenotype, enhanceable by Df(2R)ED3921/+

(Ragab et al., 2006)

osa² has adult thorax & macrochaeta phenotype, enhanceable by corto[+]/corto⁴²⁰

(Lopez et al., 2001)

Enhancer of

Statement

Reference

osa² is an enhancer of wing phenotype of sd¹

(Jin et al., 2013)

osa²/osa[+] is an enhancer of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

osa², osa[+], brm[+], brm² is an enhancer of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

osa²/osa[+] is an enhancer of wing vein | ectopic phenotype of net¹

(Marenda et al., 2004)

osa²/osa[+] is an enhancer of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is an enhancer of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is an enhancer of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is an enhancer of adult thorax & macrochaeta phenotype of corto⁴²⁰

(Lopez et al., 2001)

NOT Enhancer of

Statement

Reference

osa²/osa[+] is a non-enhancer of wing phenotype of ct^53d

(Krupp et al., 2005)

osa²/osa[+] is a non-enhancer of triple row of wing sensilla phenotype of ct^K

(Krupp et al., 2005)

osa² is a non-enhancer of eye phenotype of BEAF-32^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 2001)

Suppressor of

Statement

Reference

osa²/osa[+] is a suppressor | partially of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Nakamura et al., 2008)

osa² is a suppressor of antenna phenotype of Antp^Ns

(Ragab et al., 2006)

osa² is a suppressor of leg | ectopic phenotype of Antp^Ns

(Ragab et al., 2006)

osa²/osa[+] is a suppressor of eye phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2002)

osa²/osa[+] is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

osa²/osa[+] is a suppressor of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

osa²/osa[+] is a suppressor of antenna phenotype of Antp^Ns

(Vazquez et al., 1999)

osa²/osa[+] is a suppressor of leg | ectopic phenotype of Antp^Ns

(Vazquez et al., 1999)

osa²/osa[+] is a suppressor of antenna phenotype of Antp^Ns, Pc²

(Kennison and Tamkun, 1988)

osa² is a suppressor of phenotype of Pc⁴

(Kennison and Tamkun, 1988)

NOT Suppressor of

Statement

Reference

osa² is a non-suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

osa²/osa[+] is a non-suppressor of wing phenotype of ct^53d

(Krupp et al., 2005)

osa²/osa[+] is a non-suppressor of triple row of wing sensilla phenotype of ct^K

(Krupp et al., 2005)

osa² is a non-suppressor of eye phenotype of BEAF-32^UAS.cYa, Scer\GAL4^GMR.PS

(Yamaguchi et al., 2001)

Other

Statement

Reference

γTub23C^Pl-2, osa² has wing phenotype

(Vázquez et al., 2008)

Kr^If-1, osa²/osa[+] has eye phenotype

(Sollars et al., 2003)

Kr^If-1, osa² has eye phenotype

(Sollars et al., 2003)

brm², osa² has wing phenotype

(Vazquez et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of osa² enhances the scalloped wing phenotype seen in sd¹ mutant males.

(Jin et al., 2013)

Flies heterozygous for osa² show suppression of the Scer\GAL4^GMR.PS, Dref^Scer\UAS.cSa severe rough eye phenotype; eyes are modestly rough and fused ommatidia are not apparent.

(Nakamura et al., 2008)

γTub23C^Pl-2 results in a held-out wing phenotype in double heterozygous combination with osa² (39% penetrance).

(Vázquez et al., 2008)

The held out wing phenotype is enhanced from 2% penetrance in osa²/+ single mutants to 65% in Df(2R)ED3921/+; osa²/+ flies and 100% in Df(2R)ED3921; osa²/+ flies. The wing vein phenotype of Df(2R)ED3921 single mutants is enhanced by osa², resulting in loss of L5 up to the posterior crossvein. The osa² mutation causes an 87% suppression of the Antp^Ns homeotic transformation.

(Ragab et al., 2006)

10+/-2% of Kr^If-1/+ flies born to osa²/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers.

(Sollars et al., 2003)

osa²/+ suppresses the rough eye phenotype due to Scer\GAL4^ey.PH; asf1^Scer\UAS.cMa.

(Moshkin et al., 2002)

An osa²/+ background significantly suppresses the rough eye phenotype found in flies expressing Dref^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PS.

(Hirose et al., 2001)

corto⁴²⁰ osa² double heterozygotes give a higher percentage of thoracic macrochaetae defects than expected for an additive phenotype, indicating a synergistic interaction.

(Lopez et al., 2001)

Enhances the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies.

(Staehling-Hampton et al., 1999)

brm² osa² double heterozygotes have held-out wings in 97% of cases, a phenotype which is rarely seen in either single heterozygote. Antp^73b/+ flies show transformation of antenna to leg. This phenotype is not suppressed by one copy of osa². Antp^Ns/+ flies show transformation of antenna to leg. This phenotype is significantly suppressed by one copy of osa². Expression of Antp^hs.PHTA during the larval stages results in antenna to leg transformations. This phenotype is not affected if the animals are also carrying osa².

(Vazquez et al., 1999)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by osa².

(Maixner et al., 1998)

Suppresses the antenna to leg transformation phenotype of Pc², Antp^Ns/+. Causes between 50% and 100% suppression of the Pc⁴/+ extra sex combs phenotype.

(Kennison and Tamkun, 1988)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

osa^E65

(Staehling-Hampton et al., 1999)

osa^ksh-11

(Staehling-Hampton et al., 1999)

osa^ksh-12

(Staehling-Hampton et al., 1999)

osa^ksh-13

(Staehling-Hampton et al., 1999)

osa^pjc-3

(Staehling-Hampton et al., 1999)

osa^Arv1

(Talbert and Garber, 1994)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

3616

107130

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Osa²

(Secombe et al., 2007)

osa²

(Hong and Choi, 2016, Bengani et al., 2013, Jin et al., 2013, Monribot-Villanueva et al., 2013, Vasanthi et al., 2013, Zeng et al., 2013, Nakayama et al., 2012, Popkova et al., 2012, Curtis et al., 2011, Di Stefano et al., 2011, Salzer et al., 2010, Smulders-Srinivasan et al., 2010, Nakamura et al., 2008, Vázquez et al., 2008, Chang et al., 2007, Hirose et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (42)

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