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General Information
Symbol
Dmel\osk1
Species
D. melanogaster
Name
FlyBase ID
FBal0013303
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
osk54
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Insertion of seven bases including an in frame stop codon. Translation terminates at aa180.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nonsense mutation.

Nonsense codon in the amino terminal region.

An insertion of GTGAGTAG at 861A causes a novel stop codon at codon 180. Nucleotide substitution: G652A (silent substitution).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

osk1/Df(3R)p-XT103 females lay fewer eggs per day than wild type.

Embryos from osk1/Df(3R)p-XT103 mutant mothers exhibit an increase in the number of pole cells.

Stage 10b oocytes in osk1/osk5 females do not have long F-actin projections in the posterior region, in contrast to wild type.

The progeny of osk1/+ develop no germline (grandchildless phenotype) at very low phenotype. Stage 11 embryos have an average of 23 pole cells, reduced from an average of 33 pole cells seen in wildtype embryos.

osk1/Df(3R)p-XT103 oocytes show preferential uptake of the FM4-64 dye at the oocyte posterior at stage 8 (as occurs in wild-type oocytes), but in stage 10 mutant oocytes, FM-64 dye uptake is distributed uniformly around the oocyte cortex.

Embryos from osk9/osk1 mothers have a typical posterior group phenotype with 0-4 denticle belts.

In stage 9 oocytes from osk1/Df(3R)p-XT103 females, the accumulation of microtubule plus ends in the posterior of the oocyte is weakened or fails to occur.

oskM139L; osk1/Df(3R)p-XT103 embryos express only the long osk isoform. These embryos show patterning defects and a "twisted phenotype". Muscle development is defective with embryos showing a dramatic reduction in the number of muscles, a large number of unfused myoblasts and defects in muscle morphology.

Embryos derived from osk1/+ females have an average of 25 pole cells/embryo (compared to 33 in the wild-type).

One copy of osk1 causes the formation of 10-15 pole cells.

Strong abdominal defect.

Lack posterior body pattern elements and germ cells.

Absence of posterior pole plasm, polar granules and pole cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

osk1 has grandchildless | dominant phenotype, enhanceable by Laspy45

Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

osk[+]/osk1 is an enhancer of grandchildless | dominant phenotype of Laspy45

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

osk1 has ovary phenotype, enhanceable by Laspy45

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)1010/E(To)10[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)1212/E(To)12[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)13[+]/E(To)1313

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)11/E(To)1[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)4[+]/E(To)44

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)55/E(To)5[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)66/E(To)6[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)8[+]/E(To)88

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by E(To)9[+]/E(To)99

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by Rab11ETo11/Rab11[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by Rab11[+]/Rab11ETo3

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by magoETo2/mago[+]

Tm1el4/Tm1eg9, osk[+]/osk1 has embryonic abdominal segment phenotype, enhanceable by vasETo14/vas[+]

Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

osk[+], osk1, E(To)1212, E(To)12[+] is an enhancer of embryonic/first instar larval cuticle phenotype of Tm1el4/Tm1eg9

Other
Additional Comments
Genetic Interactions
Statement
Reference

osk1/+ females lacking both copies of Laspy45 show a penetrant grandchildless phenotype, with 20% of offspring displaying a complete absence of ovaries, and another 25% show only a single ovary.

Stage 11 embryos from osk1/+ females lacking both copies of Laspy45 develop an average of 12 pole cells, much reduced compared to osk1/+ animals.

Laspy45, osk1/Laspy41 females have a grandchildless phenotype, which is rescued by expression of LaspL.Scer\UAS.P\T under the control of Scer\GAL4otd. The number of pole cells is partially rescued, increasing to 25 pole cells in stage 11 embryos.

The grandchildless phenotype of Laspy45, osk1/Laspy41 females is not rescued by expression of LaspWA.Scer\UAS.P\T or LaspS.Scer\UAS.P\T under the control of Scer\GAL4otd. There is no increase in the number of pole cells observed in these embryos.

armi1 osk1 and armi72.1 osk1 double mutant ovaries have microtubule defects that are cytologically identical to the armi single mutant phenotype.

Embryos derived from par-1k06323/par-1k05603 ; osk1/+ females have an average of 3 pole cells/embryo (compared to 33 in the wild-type), and 42% of the embryos lack a germline.

66% of eggs derived from Tm1eg9 +/Tm1el4 osk1 females do not develop into viable larvae. In 44% of the dead eggs, embryogenesis does not commence, or is terminated before cuticle formation, while 56% of the dead eggs have a characteristic "posterior group" phenotype with missing abdominal segments. The average number of abdominal segments per embryo is 0.69 (compared to the wild-type 8).

Xenogenetic Interactions
Statement
Reference

Expression of Dimm\oskimm in embryos from osk1/Df(3R)p-XT103 mothers rescues the body patterning and pole cell formation defects of these mutant embryos; fewer pole cells form than in wild-type embryos, but they are functional. The progression of polar granule development in Dimm\oskimm; osk1/Df(3R)p-XT103 embryos is like that seen in D. melanogaster until the cellular blastoderm stage, when the smaller granules accumulate in one area of the cell, so that by gastrulation each pole cell contains a single large aggregate of granule material, like that seen in D. immigrans embryos.

Complementation and Rescue Data
Not rescued by
Comments

Expression of oskt.WT in osk1/Df(3R)p-XT103 mutant mothers results in embryos containing an average of 24 pole cells, as in wild-type.

Maternal oskstop.Scer\UAS.P\T + Scer\GAL4mat.αTub67C.T:Hsim\VP16 fails to rescue the posterior group phenotype seen in cuticles of embryos laid by osk9/osk1 mothers.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Abnormal localization of osk mRNA.

Strong allele of osk. The abdominal phenotype of osk can be rescued by cytoplasmic transplantation of wild type posterior pole plasm into the abdominal regions of mutant embryos.

Prevents the posterior accumulation of G-iα65A protein.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (62)