Laspy45, osk1/Laspy41 females have a grandchildless phenotype, which is rescued by expression of LaspL.Scer\UAS.P\T under the control of Scer\GAL4otd. The number of pole cells is partially rescued, increasing to 25 pole cells in stage 11 embryos.
The grandchildless phenotype of Laspy45, osk1/Laspy41 females is not rescued by expression of LaspWA.Scer\UAS.P\T or LaspS.Scer\UAS.P\T under the control of Scer\GAL4otd. There is no increase in the number of pole cells observed in these embryos.
66% of eggs derived from Tm1eg9 +/Tm1el4 osk1 females do not develop into viable larvae. In 44% of the dead eggs, embryogenesis does not commence, or is terminated before cuticle formation, while 56% of the dead eggs have a characteristic "posterior group" phenotype with missing abdominal segments. The average number of abdominal segments per embryo is 0.69 (compared to the wild-type 8).
Expression of Dimm\oskimm in embryos from osk1/Df(3R)p-XT103 mothers rescues the body patterning and pole cell formation defects of these mutant embryos; fewer pole cells form than in wild-type embryos, but they are functional. The progression of polar granule development in Dimm\oskimm; osk1/Df(3R)p-XT103 embryos is like that seen in D. melanogaster until the cellular blastoderm stage, when the smaller granules accumulate in one area of the cell, so that by gastrulation each pole cell contains a single large aggregate of granule material, like that seen in D. immigrans embryos.