Somatic gonadal precursors (SGPs) are specified normally, but primordial germ cells are absent in embryos derived from oskCE4/osk8 females. The SGPs compact into spherical 'gonads' even in the absence of the germ cells.
Embryos lack pole cells. Large amounts of the protein encoded by vas are expressed in early stages of oogenesis. The protein encoded by vas fails to distribute asymmetrically. Cleavage embryos have uniform distribution of the protein encoded by vas, which disappears by early gastrulation.
Hemizygous embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. Posterior localization of vas and CycB transcripts is completely abolished at 29oC and at 18oC CycB transcripts alone are localized at the posterior pole.
Embryos that are derived from oskCE4/osk8 females and are zygotically homozygous for enaC14-06 show defects or delays in somatic gonadal precursor cluster adhesion at stage 13 and fail to complete gonad compaction, resulting in extended or split gonads at later stages, as occurs in enaC14-06 single mutant embryos.