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General Information
Symbol
Dmel\osk8
Species
D. melanogaster
Name
FlyBase ID
FBal0013310
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
osk301, oskar301
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C8936758T
Reported nucleotide change:
C1941T
Amino acid change:
P424S | osk-PA; P286S | osk-PC; P93S | osk-PD
Reported amino acid change:
P424S
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: P424S. Nucleotide substitution: C1941T.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Cap cells are organized as a cluster in the ovaries from offspring of osk8/osk8 mothers, similarly to wild type.
Somatic gonadal precursors (SGPs) are specified normally, but primordial germ cells are absent in embryos derived from oskCE4/osk8 females. The SGPs compact into spherical 'gonads' even in the absence of the germ cells.
In embryos laid by osk8/oskCE4 mothers, compaction of somatic gonadal precursors in parasegment 10 (part of embryonic gonadal mesoderm) occurs normally, even though these embryos lack germ cells.
The gonadal mesoderm coalesces normally in embryos derived from osk8/oskCE4 females, even though the embryos lack germ cells.
Primordial germ cells (PGCs) are absent in the lumen of the posterior midgut (PMG).
Primordial germ cells transplanted into a pole-cell-less osk8 host migrate according to the host's developmental stage.
Temperature-sensitive allele.
Embryos derived from homozygous females do not contain pole cells.
At the restrictive temperature germline and abdomen formation is affected. At the permissive temperature germline defects remain but abdomen develops normally.
Lack posterior body pattern elements and germ cells.
Temperature sensitivity is restricted to the abdominal phenotype: at the restrictive temperature embryos lack abdomens.
Embryos lack pole cells. Large amounts of the protein encoded by vas are expressed in early stages of oogenesis. The protein encoded by vas fails to distribute asymmetrically. Cleavage embryos have uniform distribution of the protein encoded by vas, which disappears by early gastrulation.
Hemizygous embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. Posterior localization of vas and CycB transcripts is completely abolished at 29oC and at 18oC CycB transcripts alone are localized at the posterior pole.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Embryos that are derived from oskCE4/osk8 females and are zygotically homozygous for enaC14-06 show defects or delays in somatic gonadal precursor cluster adhesion at stage 13 and fail to complete gonad compaction, resulting in extended or split gonads at later stages, as occurs in enaC14-06 single mutant embryos.
The gonadal mesoderm fails to coalesce in foi20.71 oskCE4/foi16.33 embryos that are derived from osk mutant (foi20.71 oskCE4/osk8) females.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Normal localization of osk mRNA.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (48)