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General Information
Symbol
Dmel\otu10
Species
D. melanogaster
Name
FlyBase ID
FBal0013357
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Comment:

The 3' splice site of intron 5 has been eliminated.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The 3' splice site of intron 5 has been eliminated.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous females lack egg chambers. XY pseudofemales contain gonads that are similarly agametic. 90% of pseudoovaries lack egg cysts with the rest containing fewer than five chambers. Pseudofemales carrying a duplication of the otu gene have more than 90% ovaries with tumorous egg cysts.

Ovaries are devoid of egg chambers. Numbers and morphology of embryonic germ cells during stages when pole cells invaginate and form the embryonic gonads are no different to wild type. Larval ovaries are morphologically indistinguishable from wild type, gross aberrations have not yet occurred. By late pupae gonads fail to produce egg chambers and germ cells that are present fail to differentiate. otu10 homozygotes and otu1/Df(1)otu-PΔ1 transheterozygotes carrying otu98.hs have relatively large ovaries containing many tumorous egg chambers.

Chamberless ovaries. Transheterozygotes with otu13 produce ovaries containing predominantly tumorous egg cysts.

Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu14) and 86.6% (otu12) of mutant males.

Homozygous ovarioles have quiescent oogonia.

Quiescent allele; homozygous and hemizygous ovarioles contain vitellaria that lack developing follicles.

quiescent; ovarioles lack germ cells

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

otu10 has female sterile phenotype, non-suppressible by SxlM1

Phenotype Manifest In
Suppressed by
Statement
Reference

otu10 has ovary phenotype, suppressible by SxlM1

Additional Comments
Genetic Interactions
Statement
Reference

otu10 causes severe germ cell loss in XY traF.Hsp83 pseudofemales and 4 out of 5 pseudo-ovaries are completely agametic.

SxlM1 incompletely suppresses the otu10/otu13 mutant phenotype, ovaries are at a more advanced stage of oogenesis but females are still completely sterile.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

otu10 is partially rescued by otu423

otu10 is partially rescued by otu627

otu10 is partially rescued by otuhs.PN

otu10 is partially rescued by otu98.hs

Not rescued by
Comments

One or two copies of P{hs-otu.N} cause substantial morphological suppression but only partial suppression of sterility. otu10 homozygotes and otu1/Df(1)otu-PΔ1 transheterozygotes carrying otu98.hs have relatively large ovaries containing many tumorous egg chambers.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Mohler.

Comments
Comments

Quiescent (QUI) class allele. The ovarian phenotype of flies transheterozygous for otu10 and a number of other otu mutations has been analysed.

Shows a dominant interaction with ovoD2 and ovoD3 : more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles.

Severe mutation that eliminates the function of both the 98kD and 104kD isoforms.

QUI class of otu allele. P{otu-98} fails to restore detectable levels of fertility, P{otu-104} completely restores fertility.

Severe otu allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
fs(1)12-4474
otu10
Name Synonyms
Secondary FlyBase IDs
    References (12)