Allele Dmel\otu¹⁷

General Information
Symbol	Dmel\otu¹⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0013364
Feature type	allele	Associated gene	Dmel\otu

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (King et al., 1986, Storto and King, 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Deletion of part of the coding region (Mulligan et al., Mol Cell Biol 8: 1481-1488). (Geyer et al., 1993) 2 kb deletion
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective \| female (Tirronen et al., 1993) female sterile (Sass et al., 1995, Spradling et al., 1994) female sterile \| recessive (Geyer et al., 1993, King et al., 1986)
Phenotype Manifest In
	ovariole (King et al., 1986, Storto and King, 1987, Pauli et al., 1993) ovary (Geyer et al., 1993)
Detailed Description
	Statement Reference Ovaries contain tumorous egg cysts. (Geyer et al., 1993) Spermatogenesis normal but mating behaviour fails because wild type females refuse to react to the courtship attempts of mutant males. Effect is not absolute, and depends on allele (there is a strong correlation between male sterility and severity of impairment in the female phenotype) and varies between affecting 6.7% (otu¹⁴) and 86.6% (otu¹²) of mutant males. (Tirronen et al., 1993) 92% of homozygous ovarioles have quiescent oogonia, and 8% have tumorous oogonia. (Storto and King, 1987) Quiescent allele; homozygous and hemizygous ovarioles usually contain vitellaria that lack developing follicles, 8% or less of the oogonia are tumour-generating. (King et al., 1986) quiescent; ovarioles lack germ cells
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Sxl^f4/Sxl^f2, otu¹⁷ has female sterile \| dominant phenotype (Sun and Cline, 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference otu[17] dominantly suppresses the ability of Wolbachia to suppress Sxl[f4] defects in oogenesis. Almost no Sxl[f2]/Sxl[f4] females make eggs if they also carry otu[17]/+. Most egg chambers in these females are tumorous. The phenotype of a reduction in recombination across the y-sn interval on the X chromosome that is seen in Sxl[M1,f3]/Sxl[fs3] females is enhanced by otu[17]/+ ; there is a further reduction in map distance in the double mutants. In addition, the rate of X chromosome nondisjunction is increased eightfold. Sxl[M1,f3], otu[17] double heterozygotes show reduced recombination across the y-sn interval on the X chromosome compared to wild type, with the reduction being stronger than the weak reduction seen in Sxl[M1,f3] single heterozygotes. (Sun and Cline, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	otu^SuWs (Sun and Cline, 2009)
Rescued by	otu¹⁷ is rescued by otu^+t5.4 (Comer et al., 1992)
Comments	Rescued by otu^+t5.4: fertility, normal ovarian development and normal 3.2kb message expressed in ovaries is restored. (Comer et al., 1992)
Stocks ( 1 )
Bloomington	38431 y¹ w^* otu¹⁷/FM7a; P{ovo-lacZ.Δap}S1
Notes on Origin
Discoverer	Digan.

Comments
	Mutation reduces the ability of Sxl^cF1.otu to induce female Sxl⁺ RNA splicing in the male germline: number of autoregulatory cysts in testes is much reduced. (Hager and Cline, 1997) QUI class of otu allele. P{otu-98} fails to restore detectable levels of fertility, P{otu-104} completely restores fertility. (Sass et al., 1995) Likely to be a null mutation. (Geyer et al., 1993) Shows a dominant interaction with ovo^D2 and ovo^D3: more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. There is no apparent correlation with strength of otu allele and its interaction with the ovo alleles. (Pauli et al., 1993) One of QUI class of alleles. (Comer et al., 1992) Quiescent (QUI) class allele. The ovarian phenotype of flies transheterozygous for otu¹⁷ and a number of other otu mutations has been analysed. (Storto and King, 1987)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	1401 (Mohler and Carroll, 1984) fs1401 (King et al., 1986) fs(1)1401 (Spradling et al., 1994, ) otu¹⁷ (Sun and Cline, 2009)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Sun and Cline, 2009, Genetics 181(4): 1291--1301 Effects of Wolbachia infection and ovarian tumor mutations on Sex-lethal germline functioning in Drosophila. [FBrf0207689] Andrews and Oliver, 2002, Genetics 160(2): 537--545 Sex determination signals control ovo-B transcription in Drosophila melanogaster germ cells. [FBrf0144897] Hager and Cline, 1997, Development 124(24): 5033--5048 Induction of female Sex-lethal RNA splicing in male germ cells: implications for Drosophila germline sex determination. [FBrf0100057] Sass et al., 1995, Dev. Biol. 167(1): 201--212 The ovarian tumor protein isoforms of Drosophila melanogaster exhibit differences in function, expression, and localization. [FBrf0080371] Oliver et al., 1994, Development 120(11): 3185--3195 Function of Drosophila ovo⁺ in germ-line sex determination depends on X-chromosome number. [FBrf0074069] Spradling et al., 1994, Yb. Carnegie Instn, Wash. 81: 189--201 The amplification and expression of Drosophila chorion genes. [FBrf0082629] Geyer et al., 1993, Genetics 133(2): 265--278 Genetic and molecular characterization of P-element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype. [FBrf0058557] Pauli et al., 1993, Development 119(1): 123--134 The role of the ovarian tumor locus in Drosophila melanogaster germ line sex determination. [FBrf0065527] Tirronen et al., 1993, Rouxs Arch. Dev. Biol. 202(5): 306--311 Effect of otu mutations on male fertility and spermatogenesis in Drosophila melanogaster. [FBrf0059321] Comer et al., 1992, Gene 118: 171--179 Identification of a genomic DNA fragment containing the Drosophila melanogaster ovarian tumor gene (otu) and localization of regions governing its expression. [FBrf0056085] Storto and King, 1987, Rouxs Arch. Dev. Biol. 196(4): 210--221 Fertile heteroallelic combinations of mutant alleles of the otu locus of Drosophila melanogaster. [FBrf0047279] King et al., 1986, Dev. Genet. 7: 1--20 Complementation between alleles at the ovarian tumor locus of Drosophila melanogaster. [FBrf0044177] Mohler and Carroll, 1984, D. I. S. 60: 236--241 Report of new mutants. [FBrf0040657]

Allele Dmel\otu17

Allele Dmel\otu¹⁷