Allele Dmel\ovo^D1

General Information
Symbol	Dmel\ovo^D1	Species	D. melanogaster
Name		FlyBase ID	FBal0013375
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\ovo

Allele class	antimorph
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	antimorph (Oliver et al., 1990, Lindsley and Zimm, 1992, Chou et al., 1993, Mevel-Ninio et al., 1996)
Mutagen	ethyl methanesulfonate (Lindsley and Zimm, 1992)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: K1282M. (Andrews and Oliver, 1996) Amino acid replacement: K79M. Nucleotide substitution: A1282T. Mutation creates a new in frame AUG codon in the open reading frame in exon 2. (Mevel-Ninio et al., 1996)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Mevel-Ninio et al., 1996, Mevel-Ninio et al., 1991) female sterile \| dominant (Oliver et al., 1990, Andrews et al., 1998, Campos-Ortega, 1983, Kim et al., 1994, Clancy et al., 2001, Oliver et al., 1987, Mevel-Ninio et al., 1989)
Phenotype Manifest In
	egg chamber (Oliver et al., 1990, Andrews et al., 2000, Mevel-Ninio et al., 1991) ovary (Andrews et al., 2000, Extavour and Garcia-Bellido, 2001) stage S4 oocyte (Sahut-Barnola and Pauli, 1999) germ cell \| female (Mevel-Ninio et al., 1996) germ cell (Lindsley and Zimm, 1992)
Detailed Description
	Statement Reference The presence of ovo^D1 has no effect on the dl¹ phenotype. (Campos-Ortega, 1983) Mutant gene activity works in opposition to wild type activity. Viable. Male germ line is fertile, female germ line has no vitellogenic egg chambers. (Oliver et al., 1987) The egg chambers of heterozygous females fail to initiate vitellogenesis, and no eggs are laid. The soma is wild-type. Females heterozygous for both ovo^D1 and snf¹ have germ cells with a male-like morphology. (Oliver et al., 1990) Heterozygous females do not lay eggs, egg chambers form but degenerate before vitellogenic stages. (Mevel-Ninio et al., 1991) Oogenesis in heterozygous females is mainly arrested prior to stage 4. (Granadino et al., 1992) Germ-cell arrest. (Lindsley and Zimm, 1992) In heterozygous females oogenesis is arrested prior to or at stage 4. (Mevel-Ninio et al., 1996) Females carrying ovo^D1 in a wild-type or ovo^-/+ background show dominant female sterility. Heterozygous females contain no mature eggs. (Andrews et al., 1998) Oogenesis stops at about stage 4 in heterozygous females. (Sahut-Barnola and Pauli, 1999) Heterozygous females have small ovaries bearing only early egg chambers. (Andrews et al., 2000) Heterozygous females have an extended life-span compared to controls. (Clancy et al., 2001) The frequency of gypsy insertion into the ovo locus in a flam permissive background is higher in ovo^D1/+ females than in wild-type females. gypsy insertions in ovo^D1 females occur during most stages of germ-line development, in contrast to wild-type females where insertions occur only in late stages. (Labrador and Corces, 2001) Heterozygous adult females have atrophied ovaries containing some germ cells but lacking vitellogenic egg chambers. Wild-type clones made in the ovaries of a ovo^D1 female, are significantly larger than clones of heterozygous mutant cells. This size difference is greater in clones initiated at 48 hours after egg laying (AEL) than 2 hours AEL. (Extavour and Garcia-Bellido, 2001)
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference ovo^D1 chico¹ double heterozygotes live as long as chico¹/+ single heterozygotes and significantly longer than ovo^D1/+ single heterozygotes. (Clancy et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Not rescued by	ovo^D1 is not rescued by ovo^S-7.2 (Mevel-Ninio et al., 1991)
Comments	ovo^S-7.2 cannot rescue the sterility of heterozygous females. (Mevel-Ninio et al., 1991)
Stocks ( 4 )
Bloomington	1309 ovo^D1 v²⁴/C(1)DX, y¹ w¹ f¹ 1813 w^* ovo^D1 v²⁴ P{FRT(w^hs)}101/C(1)DX, y¹ f¹/Y; P{hsFLP}38
Kyoto	106273 ovo^D1 v²⁴/C(1)DX, y¹ w¹ f¹ 106517 w^* ovo^D1 v²⁴ P{FRT(w^hs)}101/C(1)DX, y¹ f¹/Y; P{hsFLP}38
Notes on Origin
Discoverer	Komitopoulou.

Comments
	The dominant ovo^D1 mutation has been reverted to a recessive loss of function mutation by insertion of a gypsy element. (Mevel-Ninio et al., 1991) The ovo^D1 allele has been cloned and transposed to several regions of the genome in P element vectors to facilitate the generation of germ line mosaics for autosomal mutations. (Chou et al., 1993) Parental strain: fs(1)A273. (Mevel-Ninio et al., 1996)
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Fs(1)K1237 (Szabad, 1998, Mathe et al., 1998, Campos-Ortega, 1983) fs(1)K1237 (Perrimon et al., 1986) ovo^D1 ovo^D (Aigaki et al., 2002, Cox et al., 2001, Yamamoto and Nakano, 1999, Fuyama, 1994, Shapiro and Anderson, 2006) Ovo^D (Perrimon et al., 1986)
Name Synonym
Secondary FlyBase IDs

References ( 47 )

Generate a list of	All references relating to this allele ( 47 )

List References by type	Research paper ( 37 ) Review ( 4 ) Abstract ( 5 ) Book ( 1 )
Recent research papers ( 1 )
	Shapiro and Anderson, 2006, Development 133(8): 1467--1475 Drosophila Ik2, a member of the IκB kinase family, is required for mRNA localization during oogenesis. [FBrf0190320] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2006 Show reviews ...

Allele Dmel\ovoD1

Allele Dmel\ovo^D1