Allele Dmel\ovo^D2

General Information
Symbol	Dmel\ovo^D2	Species	D. melanogaster
Name		FlyBase ID	FBal0013376
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\ovo

Allele class	antimorph
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	antimorph (Oliver et al., 1990, Wei et al., 1991, Garfinkel et al., 1992, Lindsley and Zimm, 1992, Chou et al., 1993, Mevel-Ninio et al., 1996)
Mutagen	ethyl methanesulfonate (Kerridge and Dura, 1982, Garfinkel et al., 1992)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: V176M. Nucleotide substitution: G1572A. Mutation creates a new in-frame AUG codon in the open reading frame in exon 2. (Mevel-Ninio et al., 1996)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Mevel-Ninio et al., 1996, Mevel-Ninio et al., 1991, Mevel-Ninio et al., 1995) female sterile \| dominant (Wei et al., 1991, Oliver et al., 1987, Andrews et al., 1998, Khila et al., 2003, Mevel-Ninio et al., 1989, Unezaki et al., 2004, Oliver et al., 1990, Kerridge and Dura, 1982) male fertile (Kerridge and Dura, 1982)
Phenotype Manifest In
	egg chamber (Wei et al., 1991, Pauli et al., 1995, Oliver et al., 1990) germ cell \| female (Mevel-Ninio et al., 1996) ovary (Pauli et al., 1995, Oliver et al., 1990) egg (Lindsley and Zimm, 1992) dorsal appendage (Oliver et al., 1990) chorion (Oliver et al., 1990)
Detailed Description
	Statement Reference Heterozygous females are completely sterile, producing only a few eggs throughout their lifetime, all of which fail to develop. (Kerridge and Dura, 1982) Mutant gene activity works in opposition to wild type activity. Viable. Male germ line is fertile, female germ line has permeable eggs. (Oliver et al., 1987) Most of the egg chambers of heterozygous females fail to initiate vitellogenesis. The more advanced egg chambers often show a variety of abnormalities, such as permeability, flaccidity, absence of the anterior end of the chorion, fused dorsal appendages, egg chambers with more than 16 nuclei and occasionally, small ovarian tumours. Less than 30% of the wild-type number of eggs are laid per day. The soma is wild-type. Transheterozygotes with either ovo^M1, ovo^M2 or ovo^D1rv20 have germ cells resembling early spermatocytes. The number of vitellogenic eggs is increased by Sxl^f7,M1, Sxl^fP7B0, Sxl^fP7C2, Sxl^fLS, Sxl^M1 or Sxl^f7,M1, but not Sxl^fPb, Sxl^f9, Sxl^f2 or Sxl^fs3. Females heterozygous for both ovo^D1 and snf¹ never have vitellogenic eggs and occasionally have no germ cells. (Oliver et al., 1990) Heterozygous females are fully sterile. (Mevel-Ninio et al., 1991) Most egg chambers arrest before vitellogenesis. Fertility is restored by the presence of two copies of ovo⁺. (Wei et al., 1991) Oogenesis in heterozygous females is mainly arrested prior to stage 6. (Granadino et al., 1992) Phenotypic category: svb⁺. (Garfinkel et al., 1992) Permeable eggs. (Lindsley and Zimm, 1992) Heterozygous females lay only very few eggs. Introduction of ovo^{B.T:Ecol\lacZ} significantly increases the number of eggs laid. Laid eggs do not show any signs of development. (Mevel-Ninio et al., 1995) Most egg chambers of ovo^D2/+ heterozygous ovaries arrest around oogenic stage 6 and very few vitellogenic oocytes are found. Two types of abnormal egg chambers occur. Pseudonurse cell chambers, where the egg chambers contain more than 15 nurse cells (though not usually more than 25) contain 0-2 oocyte nuclei. The second type of egg chamber contains largely undifferentiated germ cells, of indeterminate sex. (Pauli et al., 1995) In heterozygous females oogenesis is arrested. In the presence of either P{ovo-lacZ.B} or P{ovoM-D1} fertility of heterozygous females is significantly improved, average number of eggs laid rises from 2 to 30 and 24, respectively. For P{ovo-lacZ.B} up to 10% of eggs laid develop to adults, for P{ovoM-D1} only a small fraction of eggs develop. P{ovoM373} does not detectably change the ovo^D2 phenotype. (Mevel-Ninio et al., 1996) The dominant ovo^D2 phenotype is significantly enhanced by one copy of Df(2R)vg135, Df(2R)stil-A or Df(2R)stil-B, but is not significantly enhanced by one copy of stil¹, stil⁴ or stil⁵. The dominant enhancement of the ovo^D2 phenotype by Df(2R)vg135 is partially suppressed by a transgene carrying stil⁺. This suggests that the strong interaction between Df(2R)vg135 and ovo^D2 is due to the deletion of two genes, one of which is stil. (Pennetta and Pauli, 1997) Females carrying ovo^D2 in an ovo^-/+ background show dominant female sterility. Heterozygous females contain only a few eggs. (Andrews et al., 1998)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference ovo^D2 has female sterile \| dominant phenotype, suppressible by ovo::Bole\ovo^ODP (Khila et al., 2003) ovo^D2 has female sterile \| dominant phenotype, suppressible by ovo::Bole\ovo^ODM (Khila et al., 2003) ovo^D2 has female sterile \| dominant phenotype, suppressible \| partially by Mmus\Zfp339^A.hs (Unezaki et al., 2004)
Phenotype Manifest In
Enhanced by
Statement Reference ovo^D2 has phenotype, enhanceable by snf¹ (Pauli et al., 1995)
Additional Comments
Genetic Interactions
Statement Reference The ovo^D2/+ phenotype can be partially suppressed by alleles of Sxl, enhanced by snf¹ and enhanced by alleles of otu. (Pauli et al., 1995)
Xenogenetic Interactions
Statement Reference The dominant female sterility of ovo^D2 can be fully or partially rescued by ovo::Bole\ovo^ODM or ovo::Bole\ovo^ODP, depending on the transgenic line used. (Khila et al., 2003) The fraction of ovo^D2/+ females that produce eggs is increased if the females express Mmus\Zfp339^A.hs using a 1 hour heat shock at 37^oC; 16-68% of the heat shocked females produce eggs, compared to 4% of control ovo^D2/+ females. However, almost all the eggs laid by the ovo^D2/+ females carrying Mmus\Zfp339^A.hs fail to hatch. (Unezaki et al., 2004)
Complementation & Rescue Data
Rescued by	ovo^D2 is rescued by ovo^S-7.2 (Mevel-Ninio et al., 1991)
Comments	One copy of P{ovo.S-7.2} fully rescues the sterility of heterozygous females. (Mevel-Ninio et al., 1991)
Stocks ( 3 )
Bloomington	1318 ovo^D2 v²⁴/C(1)DX, y¹ f¹ 1843 ovo^D2 v²⁴ P{FRT(w^hs)}9-2/C(1)DX, y¹ f¹/Y; P{hsFLP}38
Kyoto	106530 ovo^D2 v²⁴ P{FRT(w^hs)}9-2/C(1)DX, y¹ f¹/Y; P{hsFLP}38
Notes on Origin
Discoverer	Komitopoulou.

Comments
	Clonal analysis shows that the dominant female sterile effect of ovo^D2 is germ line autonomous. (Kerridge and Dura, 1982) Sxl alleles that have no known effect on the soma do not suppress the ovo^D2 phenotype. (Oliver et al., 1990) Intermediate ovo phenotype. (Mevel-Ninio et al., 1991) Partially suppresses the female-specific gonadal dysgenesis caused by hybrid dysgenesis at 27.5^oC. (Wei et al., 1991) Shows a dominant interaction with otu alleles: more extreme mutant phenotype as assayed by reduction in number of egg chambers at stage 10 or further developed. (Pauli et al., 1993)
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Fs(1)K1103 (Kerridge and Dura, 1982) ovo^D2 ovoD2 (Khila et al., 2003)
Name Synonym
Secondary FlyBase IDs

References ( 26 )

Generate a list of	All references relating to this allele ( 26 )

List References by type	Research paper ( 24 ) Abstract ( 1 ) Book ( 1 )
Recent research papers (0)
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Allele Dmel\ovoD2

Allele Dmel\ovo^D2