Open Close
General Information
Symbol
Dmel\Pc15
Species
D. melanogaster
Name
FlyBase ID
FBal0013565
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
PcXT109, PcXT
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Heterozygous Pc15 mutant flies exhibit ectopic sex combs at low frequency.
Homozygous germline clones do not result in female sterility or obvious defects in the oocyte.
Eye discs composed mainly of homozygous cells have a tumour phenotype.
Approximately 90% of Pc15 heterozygotes have extra sex combs.
82% of heterozygous males have ectopic sex combs on either the second or third leg.
Eye discs composed predominantly of homozygous cells (generated using the FLP/cell-lethal method) are overgrown. The mutant tissue shows defects in terminal differentiation and has defective epithelial organisation.
Homozygous neuroblast clones in the third larval instar central brain and ventral ganglion are dramatically reduced in size compared to control clones and show a dramatic reduction in mitotic activity. Homozygous neuroblast clones examined at the late third larval stage generally still contain neuroblasts (88% of clones) if examined 24 hours after clone induction, but neuroblasts are generally absent in the clones (89% of clones) 72 hours after clone induction. 59% of the mutant clones contain neuroblasts 48 hours after clone induction.
Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Pc15 show a range of dendritic defects in the third instar larva. Homozygous class IV ddaC clones show a significant reduction in dendrite branching and total dendritic length compared to controls. Analysis of mutant clones from 72 to 96 hours after egg laying (AEL) shows that mutant clones show extensive growth of major dendritic branches and some terminal branches. However, roughly a quarter of the terminal dendrites are lost in the mutant clones during this time period. Fewer terminal branching/branch growth events are seen in the mutant clones than the control clones. Axons of mutant single cell ddaC neuron clones occupy a similar fascicular position in the ventral nerve cord and show very similar arborisation patterns as wild-type controls. Axon terminals of mutant v'ada or vdab clones are also indistinguishable from wild type.
Heterozygotes show a partial transformation of wing to haltere.
Pc15 embryos from hetrozygous mothers show segregation defects in anaphase and telophase as judged by the presence of chromatin bridges. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance. Pc15 embryos also show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. There is no evidence of metaphase defects in these embryos.
Pc15 mushroom body clones show over-elaborated dendrites, but do not show similar axonal defects.
Ectopic male-specific somatic gonadal precursor (msSGP) cells are seen in both male and female Pc3/Pc15 embryos before gonad coalescence. Normal and ectopic non-sexually dimorphic somatic gonadal precursor cell (SGP) specification is also seen in these mutant embryos. At later stage in mutant male embryos, a large number of ectopic msSGPs associate with SGPs and germ cells to form a gonad. However, in most mutant female embryos msSGPs are no longer seen at later stages.
Pc15/Df(3R)ry27 mutant flies exhibit a slightly higher than wild-type average number of legs with sex combs, with a statistically insignificant average of 2.1 legs with sex combs, compared to 2.0 in Pc15/+ heterozygotes. Pc15/Df(3R)ry619 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 2.2 legs with sex combs, compared to 2.0 in Pc15/Tp(3;3)MRS heterozygotes.
Pc15/+ animals exhibit extra sex combs on the metathoracic leg, they also have a mild transformation of the fourth abdominal segment into the fifth - patches of pigmentation are seen in the anterior part of A4.
Heterozygous males have ectopic sex combs on the second leg.
Segments of homozygous larvae are transformed so they mostly represent the eighth abdominal segment and the larvae show severe head defects.
Partial transformations of second and third legs into first legs in Pc/+ heterozygotes.
The loss of the zygotic Pc causes a severe transformation of most segments towards parasegment 8 (Lewis, Nature 276: 565-570, Duncan, Developmental Order, pp 533--554, New York).
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Pc15 has visible | dominant phenotype, enhanceable by His3HisTA.H3K27A
Pc15 has visible | dominant phenotype, enhanceable by jing[+]/jing22F3
NOT Enhanced by
Statement
Reference
Pc15 has neuroanatomy defective | somatic clone phenotype, non-enhanceable by esc21/esc[+]
Suppressed by
Statement
Reference
Pc15 has visible | dominant phenotype, suppressible | partially by Caf1-55short/Caf1[+]
Pc15 has visible | dominant phenotype, suppressible | partially by Caf1[+]/Caf1-55long
NOT suppressed by
Statement
Reference
Pc15 has neuroanatomy defective | somatic clone phenotype, non-suppressible by esc21/esc[+]
Enhancer of
Statement
Reference
Pc[+]/Pc15 is an enhancer of visible | dominant phenotype of gcmPyx
Suppressor of
Statement
Reference
Pc15 is a suppressor of lethal - all die during P-stage phenotype of stx2
Pc[+]/Pc15 is a suppressor | partially of eye color defective phenotype of wPD
Pc[+]/Pc15 is a suppressor | partially of eye color defective phenotype of wPRECycA
Phenotype Manifest In
Enhanced by
Statement
Reference
Pc15 has sex comb | ectopic phenotype, enhanceable by His3HisTA.H3K27A
Pc15 has wing phenotype, enhanceable by jing[+]/jing22F3
Pc15 has haltere | ectopic phenotype, enhanceable by jing[+]/jing22F3
Pc15 has metathoracic leg phenotype, enhanceable by chm14
Pc15 has sex comb | ectopic phenotype, enhanceable by chm14
Pc15 has phenotype, enhanceable by ScmET50
Pc15 has phenotype, enhanceable by ScmR5-13
Pc15 has phenotype, enhanceable by ScmSuz302
Pc15 has sex comb | ectopic & mesothoracic leg phenotype, enhanceable by Trl[+]/Trl62
Pc15 has sex comb | ectopic & mesothoracic leg phenotype, enhanceable by Trl[+]/Trl13C
Pc15 has mesothoracic leg phenotype, enhanceable by Trl62
Pc15 has mesothoracic leg phenotype, enhanceable by Trl13C
NOT Enhanced by
Statement
Reference
Pc15 has sex comb | ectopic phenotype, non-enhanceable by Caf1[+]/Caf1-55p55-1
Pc15 has sex comb | ectopic phenotype, non-enhanceable by Caf1-55p55-2/Caf1[+]
Pc15 has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype, non-enhanceable by esc21/esc[+]
Suppressed by
Statement
Reference
Pc15 has mesothoracic leg phenotype, suppressible | partially by Caf1-55short/Caf1[+]
Pc15 has metathoracic leg phenotype, suppressible | partially by Caf1-55short/Caf1[+]
Pc15 has sex comb | ectopic phenotype, suppressible | partially by Caf1[+]/Caf1-55long
Pc15 has mesothoracic leg phenotype, suppressible | partially by Caf1[+]/Caf1-55long
Pc15 has metathoracic leg phenotype, suppressible | partially by Caf1[+]/Caf1-55long
Pc15 has sex comb | ectopic phenotype, suppressible | partially by Caf1-55short/Caf1[+]
NOT suppressed by
Statement
Reference
Pc15 has sex comb | ectopic phenotype, non-suppressible by Caf1[+]/Caf1-55p55-1
Pc15 has sex comb | ectopic phenotype, non-suppressible by Caf1-55p55-2/Caf1[+]
Pc15 has dendrite & dorsal multidendritic neuron ddaC | somatic clone phenotype, non-suppressible by esc21/esc[+]
Enhancer of
Suppressor of
Statement
Reference
Pc15 is a suppressor of eye phenotype of Scer\GAL4lz-gal4, sensUAS.cNa
Pc[+]/Pc15 is a suppressor | partially of eye phenotype of wPD
Pc[+]/Pc15 is a suppressor | partially of pigment cell phenotype of wPRECycA
Additional Comments
Genetic Interactions
Statement
Reference
The pupal stage lethality of hemizygous stuxnet2 males is suppressed by combination with Pc15 in heterozygous state.
Expression of a transgene bearing 8 copies of a mutated form of the histone repeat unit (PBac{8xHisTA.H3K27A} - comprised of 8xHis3HisTA.H3K27A and 8x wild type copies of His1HisTA.wt, His2AHisTA.wt, His2BHisTA.wt and His3HisTA.wt) in a Pc15/+ background enhances the ectopic sex comb phenotype seen in either mutant alone.
Caf1p55-1/+ does not affect the ectopic sex comb phenotype seen in Pc15 heterozygotes. Caf1p55-2/+ does not affect the ectopic sex comb phenotype seen in Pc15 heterozygotes.
The disorganised eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4 is suppressed by Pc15. Only 12.3% of Pc15/Caf1short and 30% of Pc13/Caf1long double heterozygous males have ectopic sex combs on either the second or third leg.
Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Act.PU reduces the overgrowth of Pc15 mutant eye discs.
The addition of esc21/+ does not alter the dendritic defects seen in single cell class IV dendrite arborisation (da) neuron clones that are homozygous for Pc15.
The partial transformation of wing to haltere seen in Pc15 heterozygotes is enhanced by jing22F3/+.
Mutants heterozygous for lolalk02512, lolalk11212, or lolalk07907, when also heterozygous for Pc15, exhibit a contrabithorax phenotype, which is a wing-to-haltere transformation due to ectopic expression of Ubx in the posterior part of the wing disc, often resulting in curvature of the wing. This phenotype is further enhanced in specific lolal heteroallelic viable combinations, such as lolalk02512/lolal311a, lolalk11212/lolal311a, and lolalk07907/lolal311a, when also heterozygous for Pc15.
Mutant heterozygotes show a weak suppression of the eye phenotype seen in P{UAS-lacZ.Abd-B.5F24} leading to darker eyes. In Pc15/+, wFab-7.5F24, Scer\GAL4hs.PB flies, an embryonic heat shock pulse leads to eyes darker than with wFab-7.5F24 alone.
The appearance of extra sex combs in Pc15/+ males is enhanced if the flies are also heterozygous for either Trl62 or Trl13C.
Xenogenetic Interactions
Statement
Reference
Mutant phenotype can be rescued in the presence of Mmus\Cbx2arm.PM. Larvae were not rescued to adults with Mmus\Cbx2arm.PM. Clones of Pc15 adult cells can be rescued by one copy of Mmus\Cbx2arm.PM but to an intermediate phenotype.
Complementation and Rescue Data
Comments
Mutant phenotype can be rescued in the presence of Pcarm.PM Clones of Pc15 adult cells can be completely rescued with one copy of Pcarm.PM.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Ectopic abd-A is seen throughout the A-P axis by 9 hours. Ectopic Abd-B follows the same pattern but the anterior spread is delayed. Patchy Abd-B is seen in the epidermal cells of the head region.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (46)