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General Information
Symbol
Dmel\Raf6
Species
D. melanogaster
Name
FlyBase ID
FBal0013774
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
D-rafDF903
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Class 1 allele: Unrescued embryos (lacking maternal and zygotic phl activity) fail to differentiate into structured embryos and degenerate around 7 hrs of development. Rescued embryos (carrying wild type phl from their father) miss most structures posterior to abdominal segment 6 or 7 and anteriorly a portion of the cephalopharyngeal skeleton, labral sense organ and medial tooth are deleted. fkh expression (in wild type evident in hindgut, Malpighian tubule(s) and anal pads) entirely absent from class 1 embryos. Expression of tll, hkb, hb, fkh absent from both rescued and unrescued embryos, 7th ftz stripe deleted and 6th ftz stripe variably deleted/expanded. Conclude from 0% to 20--25% EL of blastoderm embryos deleted in class 1 alleles.

Lethal phase in homozygotes is at the larval-pupal boundary. The number of larvae that pupate is greater at low temperatures. Imaginal discs of affected homozygotes are rudimentary. The imaginal ring in the salivary gland is reduced. Number of mitotic figures in larval brain squashes is dramatically reduced. Embryos derived from germ line clones display two phenotypes. One half of the embryos (those that are paternally rescued) fail to develop structures posterior to the seventh abdominal segment. They occasionally show head involution defects or U-shaped or twisted development. Cellular blastoderm stage corresponding to these embryos appears basically normal except for a small uncellularised region at the posterior pole, beneath the pole cells. One half of the embryos (those that are not paternally rescued) show a weak disorganised cuticle consisting mostly of dorsal structures. Cellular blastoderm stage corresponding to these embryos shows multiple layers of abnormal nuclei situated along the peripheral cytoplasm. The cytoplasm is distributed unevenly along their surface.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Lefevre.

Comments
Comments

Comparison of homozygotes for this allele with heterozygotes for this allele and Df(1)64c18 (deleted for phl) is the basis for amorph designation. Isolation described in Perrimon et al., 1985, Dev. Biol. 110: 480.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)