5bp deletion causing a frameshift in the C-terminal region of the protein.
Mutation within the 5' regulatory sequence.
Amino acid replacement: W238R. Previously thought to be an insertion into the promoter, but this result was not reproducible. A mutation was detected but this corresponds to a wild type polymorphism.
4kb DNA insertion.
One copy of phl12 ameliorates size defects seen in Nf1E2 mutant flies when in combination with either Ras85Dunspecified/+ or rl1/+.
phl12 flies raised at 18[o]C have a rough eye phenotype and lack many photoreceptor cells.
The percentage of lamellocytes in the circulating blood cells of third instar larvae is strongly reduced in phl12 mutants compared to wild type.
The number of photoreceptors per ommatidium is reduced in phl12/Y flies.
Hemizygous males occasionally survive at 18oC, with rough eyes.
Hemizygous flies have small eyes.
The average number of outer photoreceptor cells is reduced to 5.5 in hemizygous males. This phenotype is not altered if the flies are also homozygous for fafBX4.
Rough eye phenotype resulting from a failure of a proportion of R7 cells and outer photoreceptor cells to differentiate.
Some ommatidia lack R7 cells, other also lack one or two outer photoreceptor cells. ttk mutations have no significant effect on the severity of the rough eye phenotype.
Defects in photoreceptor cell recruitment, 70% ommatidia lack one or photoreceptor cell.
Reduction in number of photoreceptors of both the R7 and the R1-R6 class.
Adults exhibit a rough eye phenotype. Eggs laid by homozygous females are ventralised, fused and shortened dorsal appendages and the eggs are longer than wild type (comparable to eggs laid by Egfrt1 females).
Mutation has no effect on rhohs.sev rough eye phenotype.
Some homozygous females produce ventralised eggs.
Class 3 allele: no maternal effect. At 18oC homozygotes are viable, above 25oC they either do not eclose or die soon after emergence. Rough eye phenotype present at both temperatures. Ommatidia of rough eye flies show consistent absence of R7 photoreceptor.
Raf12 has visible phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102
Raf12 has lethal phenotype, enhanceable by 14-3-3εunspecified/14-3-3epsilon[+]
Raf12 has lethal phenotype, enhanceable by EK3-3unspecified/EK3-3[+]
Raf12 has visible phenotype, enhanceable by kisunspecified/kis[+]
Raf12 has lethal phenotype, enhanceable by rl[+]/rlunspecified
Raf12 has lethal phenotype, enhanceable by Egfr[+]/Egfrunspecified
Raf12 has lethal phenotype, enhanceable by Ras85D[+]/Ras85Dunspecified
Raf12 has lethal phenotype, enhanceable by Sunspecified/S[+]
Raf12 has lethal phenotype, enhanceable by Sosunspecified/Sos[+]
Raf12 has lethal phenotype, enhanceable by cnk[+]/cnkunspecified/cnkunspecified
Raf12 has lethal phenotype, enhanceable by ksrunspecified/ksr[+]
Raf12 has lethal phenotype, enhanceable by pntunspecified/pnt[+]
Raf12 has lethal phenotype, enhanceable by spenunspecified/spen[+]
Raf12 has lethal | recessive phenotype, enhanceable by sag[+]/cnksag32-3
Raf12 has lethal phenotype, enhanceable by mamXS-2295
Raf12 has lethal phenotype, enhanceable by SR3-6S-122
Raf12 has lethal phenotype, enhanceable by SR3-6XS-328
Raf12 has lethal phenotype, non-enhanceable by EK2-5[+]/EK2-5unspecified
Raf12 has lethal phenotype, non-enhanceable by EK2-7[+]/EK2-7unspecified
Raf12 has lethal phenotype, non-enhanceable by SK2-3unspecified/SK2-3[+]
Raf12 has lethal phenotype, non-enhanceable by kisunspecified/kis[+]
Raf12 has visible phenotype, suppressible | partially by Bap170hfl1/Bap170[+]
Raf12 has lethal | heat sensitive phenotype, suppressible | partially by Bap170hfl1/Bap170[+]
Raf12 has lethal phenotype, suppressible by Src42Aunspecified/Src42A[+]
Raf12 has lethal phenotype, suppressible by aopunspecified/aop[+]
Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-3022
Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-2776
Raf12 has lethal phenotype, suppressible by aopyan-XE18
Raf12 has lethal phenotype, suppressible by ER2-1E-203
Raf12 has lethal phenotype, suppressible by ER2-3XE-2542
Raf12 has lethal phenotype, suppressible by ER2-4XE-2171
Raf12 has lethal phenotype, suppressible by ER2-4XE-2866
Raf12 has lethal phenotype, suppressible by ER3-3XE-571
Raf12 has lethal phenotype, suppressible by ER3-3XE-979
Raf12 has lethal phenotype, suppressible by ER3-5XE-2335
Raf12 has lethal phenotype, suppressible by mtsXE-2202
Raf12 has lethal phenotype, suppressible by PTP-ERXE12N
Raf12 has lethal phenotype, suppressible by SR3-10S-297
Raf12 has partially lethal phenotype, suppressible by rlSem/rl+
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Src42ASu(Raf)1-1
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)43B1
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)41
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)61
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)71
Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)91
Raf12 has decreased cell number | third instar larval stage phenotype, non-suppressible by hopTum
Raf12 has lethal phenotype, non-suppressible by EK2-5[+]/EK2-5unspecified
Raf12 has lethal phenotype, non-suppressible by EK2-7[+]/EK2-7unspecified
Raf12 has lethal phenotype, non-suppressible by SK2-3unspecified/SK2-3[+]
phl[+]/Raf12 is an enhancer of visible phenotype of S6kIIUAS.Tag:MYC, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Raf12 is a non-enhancer of visible phenotype of CanBGMR.PS, Pp2B-14Dact.GMR
phl[+], Raf12, Ras85D[+], Ras85Dunspecified is a suppressor | partially of small body | pupal stage phenotype of Nf1E2
rl[+], rl1, phl[+], Raf12 is a suppressor | partially of small body | pupal stage phenotype of Nf1E2
Raf12 is a suppressor | partially of increased cell number phenotype of Scer\GAL4ptc-559.1, SlikUAS.cHa
Raf12 is a suppressor | partially of hyperplasia | adult stage phenotype of Scer\GAL4ptc-559.1, SlikUAS.cHa
Raf12 is a suppressor of melanotic mass phenotype phenotype of hopTum
Ras64Bunspecified, phl[+], Ras64B[+], Raf12 is a non-suppressor of small body | pupal stage phenotype of Nf1E2
Raf12 is a non-suppressor of hyperplasia | third instar larval stage phenotype of hopTum
Raf12 is a non-suppressor of increased cell number | third instar larval stage phenotype of hopTum
Raf12 is a non-suppressor of visible phenotype of CanBGMR.PS, Pp2B-14Dact.GMR
EY2-6AC403, Raf12 has lethal | dominant phenotype
EY2-6AC403, Raf12 has lethal phenotype
Sin3ADW1046, Raf12 has lethal | dominant phenotype
Sin3ADW1046, Raf12 has lethal phenotype
EY2-6AQR618, Raf12 has lethal | dominant phenotype
EY2-6AQR618, Raf12 has lethal phenotype
EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal | dominant phenotype
EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal phenotype
Sin3ACY886, Raf12 has lethal | dominant phenotype
Sin3ACY886, Raf12 has lethal phenotype
EY2-8xCB204, Raf12 has lethal phenotype
EY2-8xCB204, Raf12 has lethal | dominant phenotype
EY3-2xAZ145, Raf12 has lethal phenotype
EY3-2xAZ145, Raf12 has lethal | dominant phenotype
EY3-2xCF301, Raf12 has lethal phenotype
EY3-2xCF301, Raf12 has lethal | dominant phenotype
EY3-2xDF411, Raf12 has lethal phenotype
EY3-2xDF411, Raf12 has lethal | dominant phenotype
EY3-4xII790, Raf12 has lethal phenotype
EY3-4xII790, Raf12 has lethal | dominant phenotype
EY3-4x1S805, Raf12 has lethal phenotype
Raf12, retnx1N793 has lethal | dominant phenotype
EY3-4x1S805, Raf12 has lethal | dominant phenotype
EY3-5xZB970, Raf12 has lethal phenotype
EY3-5xZB970, Raf12 has lethal | dominant phenotype
EY3-5xKR845, Raf12 has lethal phenotype
EY3-5xKR845, Raf12 has lethal | dominant phenotype
Raf12, retnx1N793 has lethal phenotype
14-3-3εj2B10, Raf12 has lethal | dominant phenotype
14-3-3εS-696, Raf12 has lethal | dominant phenotype
14-3-3εS-1259, Raf12 has lethal | dominant phenotype
14-3-3ε18A2, Raf12 has lethal | dominant phenotype
Raf12 has eye phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102
Raf12 has eye photoreceptor cell phenotype, enhanceable | somatic clone by lilli3E8
Raf12 has eye phenotype, enhanceable by EY2-5AH425
Raf12 has eye phenotype, enhanceable by kisunspecified/kis[+]
Raf12 has photoreceptor cell R7 phenotype, enhanceable by SKojak
Raf12 has eye phenotype, enhanceable by ER3-5XE-2335
Raf12 has eye phenotype, enhanceable by mamXS-2295
Raf12 has eye phenotype, enhanceable by SR3-6S-122
Raf12 has eye phenotype, enhanceable by SR3-6XS-328
Raf12 has eye phenotype, enhanceable by ER2-7AXE-2684
Raf12 has eye phenotype, enhanceable by ER2-7BXE-2337
Raf12 has eye phenotype, enhanceable by ER2-7BXE-2457
Raf12 has eye phenotype, enhanceable by ER3-4XE-2178
Raf12 has eye phenotype, enhanceable by ER3-2XE-2551
Raf12 has eye phenotype, enhanceable by SR3-2S-432
Raf12 has eye phenotype, enhanceable by SR3-2XS-355
Raf12 has eye phenotype, enhanceable by Taf6XS-922
Raf12 has eye phenotype, enhanceable by mtsXE-2258
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl1
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl2
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl3
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl4
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl5
Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl6
Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl6
Raf12 has eye phenotype, enhanceable by pnt07825Δ78
Raf12 has photoreceptor cell phenotype, enhanceable by pnt07825Δ78
Raf12 has phenotype, non-enhanceable by EY2-5DW1080
Raf12 has phenotype, non-enhanceable by EY3-3xBE183
Raf12 has phenotype, non-enhanceable by Su(Tpl)DI998
Raf12 has phenotype, non-enhanceable by Su(Tpl)EZ1217
Raf12 has phenotype, non-enhanceable by lilliDE884
Raf12 has phenotype, non-enhanceable by lilliDR999
Raf12 has eye phenotype, suppressible | partially by Bap170hfl1/Bap170[+]
Raf12 has photoreceptor cell phenotype, suppressible | partially by Bap170hfl1/Bap170[+]
Raf12 has rhabdomere phenotype, suppressible by S6kIID1/S6kII[+]
Raf12 has ommatidium phenotype, suppressible by S6kIID1/S6kII[+]
Raf12 has interommatidial bristle phenotype, suppressible by S6kIID1/S6kII[+]
Raf12 has eye phenotype, suppressible by PTP-ERXE-3022
Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-3022
Raf12 has photoreceptor cell phenotype, suppressible by PTP-ERXE-3022
Raf12 has ommatidium phenotype, suppressible by PTP-ER[-]
Raf12 has photoreceptor cell phenotype, suppressible by PTP-ER[-]
Raf12 has eye phenotype, suppressible by PTP-ERXE-2776
Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-2776
Raf12 has photoreceptor cell phenotype, suppressible by PTP-ERXE-2776
Raf12 has eye phenotype, suppressible by ER2-1E-203
Raf12 has eye phenotype, suppressible by ER2-3XE-2542
Raf12 has eye phenotype, suppressible by ER2-4XE-2171
Raf12 has eye phenotype, suppressible by ER2-4XE-2866
Raf12 has eye phenotype, suppressible by ER3-3XE-571
Raf12 has eye phenotype, suppressible by ER3-3XE-979
Raf12 has eye phenotype, suppressible | partially by mtsXE-2202
Raf12 has eye phenotype, suppressible by PTP-ERXE12N
Raf12 has eye phenotype, suppressible by SR3-10S-297
Raf12 has eye phenotype, suppressible by ER2-3XE-2213
Raf12 has eye phenotype, suppressible by ER3-5XE-2617
Raf12 has eye phenotype, suppressible by mtsXE-2258
Raf12 has eye photoreceptor cell phenotype, suppressible | partially by JraS82D.T92D.T107D.hs.sev
Raf12 has phenotype, non-suppressible by EY2-5DW1080
Raf12 has phenotype, non-suppressible by EY3-3xBE183
Raf12 has phenotype, non-suppressible by Su(Tpl)DI998
Raf12 has phenotype, non-suppressible by Su(Tpl)EZ1217
Raf12 has phenotype, non-suppressible by lilliDE884
Raf12 has phenotype, non-suppressible by lilliDR999
phl[+]/Raf12 is an enhancer of ommatidium phenotype of S6kIIUAS.Tag:MYC, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
phl[+]/Raf12 is an enhancer of interommatidial bristle phenotype of S6kIIUAS.Tag:MYC, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
phl[+]/Raf12 is an enhancer of rhabdomere phenotype of S6kIIUAS.Tag:MYC, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Raf12 is a non-enhancer of mesothoracic tergum phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237
Raf12 is a non-enhancer of thorax phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237
Raf12 is a non-enhancer of eye phenotype of CanBGMR.PS, Pp2B-14Dact.GMR
Raf12 is a suppressor of lamellocyte | supernumerary phenotype of hopTum
Raf12 is a suppressor of melanotic mass phenotype of hopTum
Raf12 is a suppressor of eye phenotype of Ras85DV12.sev
Raf12 is a non-suppressor of mesothoracic tergum phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237
Raf12 is a non-suppressor of thorax phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237
Raf12 is a non-suppressor of hemocyte | supernumerary phenotype of hopTum
Raf12 is a non-suppressor of embryonic/larval lymph gland phenotype of hopTum
Raf12 is a non-suppressor of eye phenotype of CanBGMR.PS, Pp2B-14Dact.GMR
SIIN, Raf12 has photoreceptor cell R7 phenotype
Raf12, rl[+]/rlSem has photoreceptor cell phenotype
The rough eye phenotype seen in phl12/Y flies is dominantly enhanced by RE-814, PDZ-GEFE-1102 and rlE-80.
Bap170hfl1/+ significantly suppresses the rough eye phenotype and loss of photoreceptor cells seen in phl12 flies at 18[o]C.
The lethality caused by phl12 at 25[o]C is partially rescued by Bap170hfl1/+.
Expression of S6kIIScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a phl12/+ background than when expressed in a wild-type background.
The eye roughness and decreased rhabdomere phenotypes seen in phl12 flies are suppressed in phl12, S6kIID1/Y flies. The male temperature sensitive lethality of phl12/Y flies is suppressed in phl12/Y, S6kIID1/Y flies.
When phl12 is added to slikScer\UAS.cHa, Scer\GAL4ptc-559.1 animals the overgrowth phenotype is partially suppressed.
At 28oC, less than 3% lamellocytes are seen in phl12 hopTum larvae. Plasmatocytes with abnormal morphology are occasionally seen in these animals; some blood cells are larger than the plasmatocytes normally seen in wild-type larvae, while others have processes or an elongated morphology. The overproliferation of blood cells seen in hopTum larvae is not suppressed by phl12. phl12 hopTum larvae have reduced melanotic tumour formation compared to hopTum larvae, but the lymph glands are still overgrown.
Heterozygous PTP-ERXE-3022, PTP-ERXE-2776 as well as deficiency alleles of PTP-ER suppress the rough eyes and loss of photoreceptor cells seen in hemizygous phl12.
Synthetic lethal with alleles of cnk.
Phenotype can be slightly rescued (60-70% ommatidia have wild-type complement of photoreceptor cells) by JraS82D.T92D.T107D.hs.sev.
The phenotype is dominantly enhanced by phyl mutants.
Reduced viability is dominantly suppressed by rlSem. Surviving flies have a reduced number of photoreceptors.
One copy of Su(phl)6, Su(phl)43B, Su(phl)9, Src42A, Su(phl)7, Su(phl)4 and Dsor1Su34B can rescue lethality at the restrictive temperature.
Acts as a dominant suppressor of the activated Ras85DV12.sev. Halving the gene dosage of pnt enhances both the rough eye and missing photoreceptor phenotypes of Dsor1XS520 and phl12.
Described in Kramers, Mutation Research 107:187 .
Allele designation of hypomorph based on observation that homozygotes die at pupal-adult interface as but heterozygotes with Df(1)64c18 die as pupae. Isolation described in Kramers et al., Mut. Res., 107: 187.