FB2020_06, released Dec 22, 2020

Allele: Dmel\Raf12

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General Information
Symbol
Dmel\Raf12
Species
D. melanogaster
Name
FlyBase ID
FBal0013780
Feature type
allele
Associated gene
Dmel\Raf
Associated Insertion(s)
Carried in Construct
Also Known As
rafHM7, DrafHM7, phl12, phlHM7, D-rafHM-7, l(1)phHM7
Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

heat sensitive hypomorphic allele - genetic evidence

(Melnick et al., 1993)
Mutagen
hycanthon methanesulfonate
(Melnick et al., 1993, Kramers et al., 1983)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      5bp deletion causing a frameshift in the C-terminal region of the protein.

      (Karim et al., 1996)

      Mutation within the 5' regulatory sequence.

      (Brand and Perrimon, 1994)

      Amino acid replacement: W238R. Previously thought to be an insertion into the promoter, but this result was not reproducible. A mutation was detected but this corresponds to a wild type polymorphism.

      (Melnick et al., 1993)

      4kb DNA insertion.

      (Ambrosio et al., 1989)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  neurofibromatosis
      modeled by Nf1E2
      (Walker et al., 2006)
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       

      One copy of phl12 ameliorates size defects seen in Nf1E2 mutant flies when in combination with either Ras85Dunspecified/+ or rl1/+.

      (Walker et al., 2006)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      phl12 flies raised at 18[o]C have a rough eye phenotype and lack many photoreceptor cells.

      (Rendina et al., 2010)

      phl12 hemizygotic males are viable at 18oC, but lethal at 25oC.

      phl12 flies show a roughened eye phenotype with a decrease in rhabdomere number.

      (Kim et al., 2006)

      The percentage of lamellocytes in the circulating blood cells of third instar larvae is strongly reduced in phl12 mutants compared to wild type.

      (Luo et al., 2002)

      The number of photoreceptors per ommatidium is reduced in phl12/Y flies.

      (Wittwer et al., 2001)

      Hemizygous males occasionally survive at 18oC, with rough eyes.

      (Rebay et al., 2000)

      Hemizygous flies have small eyes.

      (Ruden et al., 1999)

      The average number of outer photoreceptor cells is reduced to 5.5 in hemizygous males. This phenotype is not altered if the flies are also homozygous for fafBX4.

      (Isaksson et al., 1997)

      Hemizygotes exhibit absence of the R7 cell in 50% ommatidia. Hsp83e6D interact genetically with the phl12, other E(sev) Hsp83 mutations fail to interact.

      (van der Straten et al., 1997)

      Rough eye phenotype resulting from a failure of a proportion of R7 cells and outer photoreceptor cells to differentiate.

      (Karim et al., 1996)

      Some ommatidia lack R7 cells, other also lack one or two outer photoreceptor cells. ttk mutations have no significant effect on the severity of the rough eye phenotype.

      (Lai et al., 1996)

      Defects in photoreceptor cell recruitment, 70% ommatidia lack one or photoreceptor cell.

      (Peverali et al., 1996)

      Reduction in number of photoreceptors of both the R7 and the R1-R6 class.

      (Dickson et al., 1995)

      Adults exhibit a rough eye phenotype. Eggs laid by homozygous females are ventralised, fused and shortened dorsal appendages and the eggs are longer than wild type (comparable to eggs laid by Egfrt1 females).

      (Brand and Perrimon, 1994)

      Mutation has no effect on rhohs.sev rough eye phenotype.

      (Freeman, 1994)

      Some homozygous females produce ventralised eggs.

      (Lu et al., 1994)

      Class 3 allele: no maternal effect. At 18oC homozygotes are viable, above 25oC they either do not eclose or die soon after emergence. Rough eye phenotype present at both temperatures. Ommatidia of rough eye flies show consistent absence of R7 photoreceptor.

      (Melnick et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Raf12 has visible phenotype, enhanceable by R[+]/Rap1E-814

      (Baril et al., 2014)

      Raf12 has visible phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102

      (Baril et al., 2014)

      Raf12 has visible phenotype, enhanceable by rl+/rlE-80

      (Baril et al., 2014)

      Raf12 has lethal phenotype, enhanceable by 14-3-3εunspecified/14-3-3epsilon[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by EK3-3unspecified/EK3-3[+]

      (Therrien et al., 2000)

      Raf12 has visible phenotype, enhanceable by kisunspecified/kis[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by rl[+]/rlunspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by Egfr[+]/Egfrunspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by Ras85D[+]/Ras85Dunspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by Sunspecified/S[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by Sosunspecified/Sos[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by cnk[+]/cnkunspecified/cnkunspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by ksrunspecified/ksr[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by pntunspecified/pnt[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, enhanceable by spenunspecified/spen[+]

      (Therrien et al., 2000)

      Raf12 has visible | recessive phenotype, enhanceable by SKojak

      (Ruden et al., 1999)

      Raf12 has lethal | recessive phenotype, enhanceable by cnksag13L/sag[+]

      (Zhang et al., 1999)

      Raf12 has lethal | recessive phenotype, enhanceable by sag[+]/cnksag32-3

      (Zhang et al., 1999)

      Raf12 has lethal | recessive phenotype, enhanceable by sag[+]/cnkA4-2

      (Zhang et al., 1999)

      Raf12 has lethal | recessive phenotype, enhanceable by cnk16-2/sag[+]

      (Zhang et al., 1999)

      Raf12 has lethal phenotype, enhanceable by ksrS-627

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by ksrXS-337

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by mamS-639

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by mamXS-2295

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by rlS-135

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by rlXS-324

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by SR3-6S-122

      (Karim et al., 1996)

      Raf12 has lethal phenotype, enhanceable by SR3-6XS-328

      (Karim et al., 1996)
      NOT Enhanced by
      Statement
      Reference

      Raf12 has lethal phenotype, non-enhanceable by EK2-5[+]/EK2-5unspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, non-enhanceable by EK2-7[+]/EK2-7unspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, non-enhanceable by SK2-3unspecified/SK2-3[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, non-enhanceable by kisunspecified/kis[+]

      (Therrien et al., 2000)
      Suppressed by
      Statement
      Reference

      Raf12 has visible phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

      (Rendina et al., 2010)

      Raf12 has lethal | heat sensitive phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

      (Rendina et al., 2010)

      Raf12 has lethal | male | heat sensitive phenotype, suppressible by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      Raf12 has visible phenotype, suppressible by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphS-292

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphS-331

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, suppressible by alphS-355/pp2c99B[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphXS-88

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, suppressible by Src42Aunspecified/Src42A[+]

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, suppressible by aopunspecified/aop[+]

      (Therrien et al., 2000)

      Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-3022

      (Karim and Rubin, 1999)

      Raf12 has visible | recessive phenotype, suppressible by PTP-ER[-]

      (Karim and Rubin, 1999)

      Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-2776

      (Karim and Rubin, 1999)

      Raf12 has lethal phenotype, suppressible by aopyan-XE18

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER2-1E-203

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER2-1E-33

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER2-3XE-2542

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER2-4XE-2171

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER2-4XE-2866

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER3-3XE-571

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER3-3XE-979

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by ER3-5XE-2335

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by mtsXE-2202

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by PTP-ERXE12N

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by SR3-10S-297

      (Karim et al., 1996)

      Raf12 has lethal phenotype, suppressible by aopE-867

      (Karim et al., 1996)

      Raf12 has partially lethal phenotype, suppressible by rlSem/rl+

      (Brunner et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Src42ASu(Raf)1-1

      (Lu et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)43B1

      (Lu et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)41

      (Lu et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)61

      (Lu et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)71

      (Lu et al., 1994)

      Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)91

      (Lu et al., 1994)
      NOT suppressed by
      Statement
      Reference

      Raf12 has decreased cell number | third instar larval stage phenotype, non-suppressible by hopTum

      (Luo et al., 2002)

      Raf12 has lethal phenotype, non-suppressible by EK2-5[+]/EK2-5unspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, non-suppressible by EK2-7[+]/EK2-7unspecified

      (Therrien et al., 2000)

      Raf12 has lethal phenotype, non-suppressible by SK2-3unspecified/SK2-3[+]

      (Therrien et al., 2000)
      Enhancer of
      Statement
      Reference

      phl[+]/Raf12 is an enhancer of visible phenotype of S6kIIUAS.Tag:MYC, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

      (Kim et al., 2006)
      NOT Enhancer of
      Statement
      Reference

      Raf12 is a non-enhancer of visible phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

      (Sullivan and Rubin, 2002)
      Suppressor of
      Statement
      Reference

      phl[+], Raf12, Ras85D[+], Ras85Dunspecified is a suppressor | partially of small body | pupal stage phenotype of Nf1E2

      (Walker et al., 2006)

      rl[+], rl1, phl[+], Raf12 is a suppressor | partially of small body | pupal stage phenotype of Nf1E2

      (Walker et al., 2006)

      Raf12 is a suppressor | partially of increased cell number phenotype of Scer\GAL4ptc-559.1, SlikUAS.cHa

      (Hipfner and Cohen, 2003)

      Raf12 is a suppressor | partially of hyperplasia | adult stage phenotype of Scer\GAL4ptc-559.1, SlikUAS.cHa

      (Hipfner and Cohen, 2003)

      Raf12 is a suppressor of melanotic mass phenotype phenotype of hopTum

      (Luo et al., 2002)
      NOT Suppressor of
      Statement
      Reference

      Ras64Bunspecified, phl[+], Ras64B[+], Raf12 is a non-suppressor of small body | pupal stage phenotype of Nf1E2

      (Walker et al., 2006)

      Raf12 is a non-suppressor of hyperplasia | third instar larval stage phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a non-suppressor of increased cell number | third instar larval stage phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a non-suppressor of visible phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

      (Sullivan and Rubin, 2002)
      Other
      Statement
      Reference

      EY2-6AC403, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY2-6AC403, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      Sin3ADW1046, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Sin3ADW1046, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY2-6AQR618, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY2-6AQR618, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal phenotype

      (Rebay et al., 2000)

      Sin3ACY886, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Sin3ACY886, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      SBA651, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      SBA651, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-3xF6, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-3xF6, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      eyaA188, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      eyaA188, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, rlA0648 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, rlA0648 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, rlAL528 has lethal phenotype

      (Rebay et al., 2000)

      SosD120, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      SosD120, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      SosxB10, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      SosxB10, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      SU132, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      SU132, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, spenAH393 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, spenAH393 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, spenBU720 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, spenBU720 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY2-8xCB204, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY2-8xCB204, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, pntAP553 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, pntAP553 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, pntAF397 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, pntAF397 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-2xAZ145, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-2xAZ145, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-2xCF301, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-2xCF301, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-2xDF411, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-2xDF411, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-4xII790, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-4xII790, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-4x1S805, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      Raf12, retnx1N793 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-4x1S805, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-5xZB970, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-5xZB970, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      EY3-5xKR845, Raf12 has lethal phenotype

      (Rebay et al., 2000)

      EY3-5xKR845, Raf12 has lethal | dominant phenotype

      (Rebay et al., 2000)

      Raf12, retnx1N793 has lethal phenotype

      (Rebay et al., 2000)

      14-3-3εj2B10, Raf12 has lethal | dominant phenotype

      (Chang and Rubin, 1997)

      14-3-3εS-696, Raf12 has lethal | dominant phenotype

      (Chang and Rubin, 1997)

      14-3-3εS-1259, Raf12 has lethal | dominant phenotype

      (Chang and Rubin, 1997)

      14-3-3ε18A2, Raf12 has lethal | dominant phenotype

      (Chang and Rubin, 1997, Dickson et al., 1996)

      Raf12, rl2L1 has lethal | dominant phenotype

      (Dickson et al., 1996)

      Hsp839J1, Raf12 has lethal | dominant phenotype

      (Dickson et al., 1996)

      Hsp8319F2, Raf12 has lethal | dominant phenotype

      (Dickson et al., 1996)

      Raf12, spen14O1 has lethal phenotype

      (Dickson et al., 1996)

      Raf12, spen16T1 has lethal | dominant phenotype

      (Dickson et al., 1996)

      Raf12, spen16T1 has lethal phenotype

      (Dickson et al., 1996)

      S2J1, Raf12 has lethal | dominant phenotype

      (Dickson et al., 1996)

      Raf12, rl6L1 has lethal | dominant phenotype

      (Dickson et al., 1996)

      S2J1, phl[+]/Raf12 has lethal phenotype

      (Dickson et al., 1996)

      Raf12, spen16H1 has lethal phenotype

      (Dickson et al., 1996)

      Raf12, spen16H1 has lethal | dominant phenotype

      (Dickson et al., 1996)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Raf12 has eye phenotype, enhanceable by R[+]/Rap1E-814

      (Baril et al., 2014)

      Raf12 has eye phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102

      (Baril et al., 2014)

      Raf12 has eye phenotype, enhanceable by rl+/rlE-80

      (Baril et al., 2014)

      Raf12 has eye photoreceptor cell phenotype, enhanceable | somatic clone by lilli3E8

      (Wittwer et al., 2001)

      Raf12 has eye phenotype, enhanceable by eyaG130

      (Rebay et al., 2000)

      Raf12 has eye phenotype, enhanceable by EY2-5AH425

      (Rebay et al., 2000)

      Raf12 has eye phenotype, enhanceable by kisunspecified/kis[+]

      (Therrien et al., 2000)

      Raf12 has eye phenotype, enhanceable by SKojak

      (Ruden et al., 1999)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by SKojak

      (Ruden et al., 1999)

      Raf12 has eye phenotype, enhanceable by spen14O1

      (Dickson et al., 1996)

      Raf12 has eye phenotype, enhanceable by phyl1

      (Dickson et al., 1996)

      Raf12 has eye phenotype, enhanceable by phyl2

      (Dickson et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER3-5XE-2335

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ksrS-627

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ksrXS-337

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by mamS-639

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by mamXS-2295

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by rlS-135

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by rlXS-324

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by SR3-6S-122

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by SR3-6XS-328

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER2-7AXE-2684

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER2-7BXE-2337

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER2-7BXE-2457

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER3-4XE-2178

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by hhXE-2134

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by hhXE-3161

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by ER3-2XE-2551

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by SR3-2S-432

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by SR3-2XS-355

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by Taf6XS-922

      (Karim et al., 1996)

      Raf12 has eye phenotype, enhanceable by mtsXE-2258

      (Wassarman et al., 1996)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl1

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl2

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl3

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl4

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl5

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl6

      (Dickson et al., 1995)

      Raf12 has eye phenotype, enhanceable by pnt07825Δ78

      (O'Neill et al., 1994)

      Raf12 has photoreceptor cell phenotype, enhanceable by pnt07825Δ78

      (O'Neill et al., 1994)
      NOT Enhanced by
      Statement
      Reference

      Raf12 has phenotype, non-enhanceable by retnB142

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by glDZ1053

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by glER1085

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by stgD115

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by stgDD937

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by EY2-5DW1080

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by EY3-3xBE183

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by Su(Tpl)DI998

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by Su(Tpl)EZ1217

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by lilliDE884

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by lilliDR999

      (Rebay et al., 2000)

      Raf12 has phenotype, non-enhanceable by Taf4AG456

      (Rebay et al., 2000)
      Suppressed by
      Statement
      Reference

      Raf12 has eye phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

      (Rendina et al., 2010)

      Raf12 has photoreceptor cell phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

      (Rendina et al., 2010)

      Raf12 has rhabdomere phenotype, suppressible by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      Raf12 has ommatidium phenotype, suppressible by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      Raf12 has interommatidial bristle phenotype, suppressible by S6kIID1/S6kII[+]

      (Kim et al., 2006)

      Raf12 has eye phenotype, suppressible by Taf4I53

      (Rebay et al., 2000)

      Raf12 has eye phenotype, suppressible by PTP-ERXE-3022

      (Karim and Rubin, 1999)

      Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-3022

      (Karim and Rubin, 1999)

      Raf12 has photoreceptor cell phenotype, suppressible by PTP-ERXE-3022

      (Karim and Rubin, 1999)

      Raf12 has eye phenotype, suppressible by PTP-ER[-]

      (Karim and Rubin, 1999)

      Raf12 has ommatidium phenotype, suppressible by PTP-ER[-]

      (Karim and Rubin, 1999)

      Raf12 has photoreceptor cell phenotype, suppressible by PTP-ER[-]

      (Karim and Rubin, 1999)

      Raf12 has eye phenotype, suppressible by PTP-ERXE-2776

      (Karim and Rubin, 1999)

      Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-2776

      (Karim and Rubin, 1999)

      Raf12 has photoreceptor cell phenotype, suppressible by PTP-ERXE-2776

      (Karim and Rubin, 1999)

      Raf12 has eye phenotype, suppressible by ER2-1E-203

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER2-1E-33

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER2-3XE-2542

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER2-4XE-2171

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER2-4XE-2866

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER3-3XE-571

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER3-3XE-979

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible | partially by mtsXE-2202

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by PTP-ERXE12N

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by SR3-10S-297

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER2-3XE-2213

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by ER3-5XE-2617

      (Karim et al., 1996)

      Raf12 has eye phenotype, suppressible by mtsXE-2258

      (Karim et al., 1996)

      Raf12 has eye photoreceptor cell phenotype, suppressible | partially by JraS82D.T92D.T107D.hs.sev

      (Peverali et al., 1996)
      NOT suppressed by
      Statement
      Reference

      Raf12 has phenotype, non-suppressible by EY2-5DW1080

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by EY3-3xBE183

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by Su(Tpl)DI998

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by Su(Tpl)EZ1217

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by lilliDE884

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by lilliDR999

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by Taf4AG456

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by retnB142

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by glDZ1053

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by glER1085

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by stgD115

      (Rebay et al., 2000)

      Raf12 has phenotype, non-suppressible by stgDD937

      (Rebay et al., 2000)
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Raf12 is a non-enhancer of mesothoracic tergum phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237

      (Ishimaru et al., 2004)

      Raf12 is a non-enhancer of thorax phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237

      (Ishimaru et al., 2004)

      Raf12 is a non-enhancer of eye phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

      (Sullivan and Rubin, 2002)
      Suppressor of
      Statement
      Reference

      Raf12 is a suppressor of lamellocyte | supernumerary phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a suppressor of melanotic mass phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a suppressor of eye phenotype of Ras85DV12.sev

      (O'Neill et al., 1994)
      NOT Suppressor of
      Statement
      Reference

      Raf12 is a non-suppressor of mesothoracic tergum phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237

      (Ishimaru et al., 2004)

      Raf12 is a non-suppressor of thorax phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237

      (Ishimaru et al., 2004)

      Raf12 is a non-suppressor of hemocyte | supernumerary phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a non-suppressor of embryonic/larval lymph gland phenotype of hopTum

      (Luo et al., 2002)

      Raf12 is a non-suppressor of eye phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

      (Sullivan and Rubin, 2002)
      Other
      Statement
      Reference

      SIIN, Raf12 has photoreceptor cell R7 phenotype

      (Ruden et al., 1999)

      Raf12, rl[+]/rlSem has photoreceptor cell phenotype

      (Brunner et al., 1994)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The rough eye phenotype seen in phl12/Y flies is dominantly enhanced by RE-814, PDZ-GEFE-1102 and rlE-80.

      (Baril et al., 2014)

      Bap170hfl1/+ significantly suppresses the rough eye phenotype and loss of photoreceptor cells seen in phl12 flies at 18[o]C.

      The lethality caused by phl12 at 25[o]C is partially rescued by Bap170hfl1/+.

      (Rendina et al., 2010)

      Expression of S6kIIScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a phl12/+ background than when expressed in a wild-type background.

      The eye roughness and decreased rhabdomere phenotypes seen in phl12 flies are suppressed in phl12, S6kIID1/Y flies. The male temperature sensitive lethality of phl12/Y flies is suppressed in phl12/Y, S6kIID1/Y flies.

      (Kim et al., 2006)

      When phl12 is added to slikScer\UAS.cHa, Scer\GAL4ptc-559.1 animals the overgrowth phenotype is partially suppressed.

      (Hipfner and Cohen, 2003)

      At 28oC, less than 3% lamellocytes are seen in phl12 hopTum larvae. Plasmatocytes with abnormal morphology are occasionally seen in these animals; some blood cells are larger than the plasmatocytes normally seen in wild-type larvae, while others have processes or an elongated morphology. The overproliferation of blood cells seen in hopTum larvae is not suppressed by phl12. phl12 hopTum larvae have reduced melanotic tumour formation compared to hopTum larvae, but the lymph glands are still overgrown.

      (Luo et al., 2002)

      The loss of photoreceptors seen in phl12/Y flies is further enhanced by the addition of lilli3E8 clones in the eye.

      (Wittwer et al., 2001)

      Heterozygous PTP-ERXE-3022, PTP-ERXE-2776 as well as deficiency alleles of PTP-ER suppress the rough eyes and loss of photoreceptor cells seen in hemizygous phl12.

      (Karim and Rubin, 1999)

      The hemizygous phenotype is enhanced by SKojak. phl12/Y ; SKojak/+ flies have no R7 photoreceptor cells. Flies doubly mutant for phl12 and SIIN have no R7 photoreceptor cells.

      (Ruden et al., 1999)

      The lethality of phl12 is dominantly enhanced by sag13L, sag32-3, sagA4-2 or sag16-2.

      (Zhang et al., 1999)

      Synthetic lethal with alleles of cnk.

      (Therrien et al., 1998)

      Eye phenotype is enhanced by mutations in phyl and spen. Synthetic lethal in combination with alleles of spen, S, rl, Hsp83 and 14-3-3ε.

      (Dickson et al., 1996)

      Phenotype can be slightly rescued (60-70% ommatidia have wild-type complement of photoreceptor cells) by JraS82D.T92D.T107D.hs.sev.

      (Peverali et al., 1996)

      The phenotype is dominantly enhanced by phyl mutants.

      (Dickson et al., 1995)

      Reduced viability is dominantly suppressed by rlSem. Surviving flies have a reduced number of photoreceptors.

      (Brunner et al., 1994)

      One copy of Su(phl)6, Su(phl)43B, Su(phl)9, Src42A, Su(phl)7, Su(phl)4 and Dsor1Su34B can rescue lethality at the restrictive temperature.

      (Lu et al., 1994)

      Acts as a dominant suppressor of the activated Ras85DV12.sev. Halving the gene dosage of pnt enhances both the rough eye and missing photoreceptor phenotypes of Dsor1XS520 and phl12.

      (O'Neill et al., 1994)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Raf12 is rescued by RafC20

      (Brand and Perrimon, 1994)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Described in Kramers, Mutation Research 107:187 .

      (Dickson et al., 1992)

      Allele designation of hypomorph based on observation that homozygotes die at pupal-adult interface as but heterozygotes with Df(1)64c18 die as pupae. Isolation described in Kramers et al., Mut. Res., 107: 187.

      (Melnick et al., 1993)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (13)
      References (44)