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General Information
Symbol
Dmel\Raf12
Species
D. melanogaster
Name
FlyBase ID
FBal0013780
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rafHM7, DrafHM7, phl12, phlHM7, D-rafHM-7, l(1)phHM7
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

5bp deletion causing a frameshift in the C-terminal region of the protein.

Mutation within the 5' regulatory sequence.

Amino acid replacement: W238R. Previously thought to be an insertion into the promoter, but this result was not reproducible. A mutation was detected but this corresponds to a wild type polymorphism.

4kb DNA insertion.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

One copy of phl12 ameliorates size defects seen in Nf1E2 mutant flies when in combination with either Ras85Dunspecified/+ or rl1/+.

Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

phl12 flies raised at 18[o]C have a rough eye phenotype and lack many photoreceptor cells.

phl12 hemizygotic males are viable at 18oC, but lethal at 25oC.

phl12 flies show a roughened eye phenotype with a decrease in rhabdomere number.

The percentage of lamellocytes in the circulating blood cells of third instar larvae is strongly reduced in phl12 mutants compared to wild type.

The number of photoreceptors per ommatidium is reduced in phl12/Y flies.

Hemizygous males occasionally survive at 18oC, with rough eyes.

Hemizygous flies have small eyes.

The average number of outer photoreceptor cells is reduced to 5.5 in hemizygous males. This phenotype is not altered if the flies are also homozygous for fafBX4.

Hemizygotes exhibit absence of the R7 cell in 50% ommatidia. Hsp83e6D interact genetically with the phl12, other E(sev) Hsp83 mutations fail to interact.

Rough eye phenotype resulting from a failure of a proportion of R7 cells and outer photoreceptor cells to differentiate.

Some ommatidia lack R7 cells, other also lack one or two outer photoreceptor cells. ttk mutations have no significant effect on the severity of the rough eye phenotype.

Defects in photoreceptor cell recruitment, 70% ommatidia lack one or photoreceptor cell.

Reduction in number of photoreceptors of both the R7 and the R1-R6 class.

Adults exhibit a rough eye phenotype. Eggs laid by homozygous females are ventralised, fused and shortened dorsal appendages and the eggs are longer than wild type (comparable to eggs laid by Egfrt1 females).

Mutation has no effect on rhohs.sev rough eye phenotype.

Some homozygous females produce ventralised eggs.

Class 3 allele: no maternal effect. At 18oC homozygotes are viable, above 25oC they either do not eclose or die soon after emergence. Rough eye phenotype present at both temperatures. Ommatidia of rough eye flies show consistent absence of R7 photoreceptor.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Raf12 has visible phenotype, enhanceable by R[+]/Rap1E-814

Raf12 has visible phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102

Raf12 has visible phenotype, enhanceable by rl+/rlE-80

Raf12 has lethal phenotype, enhanceable by 14-3-3εunspecified/14-3-3epsilon[+]

Raf12 has lethal phenotype, enhanceable by EK3-3unspecified/EK3-3[+]

Raf12 has visible phenotype, enhanceable by kisunspecified/kis[+]

Raf12 has lethal phenotype, enhanceable by rl[+]/rlunspecified

Raf12 has lethal phenotype, enhanceable by Egfr[+]/Egfrunspecified

Raf12 has lethal phenotype, enhanceable by Ras85D[+]/Ras85Dunspecified

Raf12 has lethal phenotype, enhanceable by Sunspecified/S[+]

Raf12 has lethal phenotype, enhanceable by Sosunspecified/Sos[+]

Raf12 has lethal phenotype, enhanceable by cnk[+]/cnkunspecified/cnkunspecified

Raf12 has lethal phenotype, enhanceable by ksrunspecified/ksr[+]

Raf12 has lethal phenotype, enhanceable by pntunspecified/pnt[+]

Raf12 has lethal phenotype, enhanceable by spenunspecified/spen[+]

Raf12 has visible | recessive phenotype, enhanceable by SKojak

Raf12 has lethal | recessive phenotype, enhanceable by cnksag13L/sag[+]

Raf12 has lethal | recessive phenotype, enhanceable by sag[+]/cnksag32-3

Raf12 has lethal | recessive phenotype, enhanceable by sag[+]/cnkA4-2

Raf12 has lethal | recessive phenotype, enhanceable by cnk16-2/sag[+]

Raf12 has lethal phenotype, enhanceable by ksrS-627

Raf12 has lethal phenotype, enhanceable by ksrXS-337

Raf12 has lethal phenotype, enhanceable by mamS-639

Raf12 has lethal phenotype, enhanceable by mamXS-2295

Raf12 has lethal phenotype, enhanceable by rlS-135

Raf12 has lethal phenotype, enhanceable by rlXS-324

Raf12 has lethal phenotype, enhanceable by SR3-6S-122

Raf12 has lethal phenotype, enhanceable by SR3-6XS-328

NOT Enhanced by
Statement
Reference

Raf12 has lethal phenotype, non-enhanceable by EK2-5[+]/EK2-5unspecified

Raf12 has lethal phenotype, non-enhanceable by EK2-7[+]/EK2-7unspecified

Raf12 has lethal phenotype, non-enhanceable by SK2-3unspecified/SK2-3[+]

Raf12 has lethal phenotype, non-enhanceable by kisunspecified/kis[+]

Suppressed by
Statement
Reference

Raf12 has visible phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

Raf12 has lethal | heat sensitive phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

Raf12 has lethal | male | heat sensitive phenotype, suppressible by S6kIID1/S6kII[+]

Raf12 has visible phenotype, suppressible by S6kIID1/S6kII[+]

Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphS-292

Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphS-331

Raf12 has lethal phenotype, suppressible by alphS-355/pp2c99B[+]

Raf12 has lethal phenotype, suppressible by pp2c99B[+]/alphXS-88

Raf12 has lethal phenotype, suppressible by Src42Aunspecified/Src42A[+]

Raf12 has lethal phenotype, suppressible by aopunspecified/aop[+]

Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-3022

Raf12 has visible | recessive phenotype, suppressible by PTP-ER[-]

Raf12 has visible | recessive phenotype, suppressible by PTP-ERXE-2776

Raf12 has lethal phenotype, suppressible by aopyan-XE18

Raf12 has lethal phenotype, suppressible by ER2-1E-203

Raf12 has lethal phenotype, suppressible by ER2-1E-33

Raf12 has lethal phenotype, suppressible by ER2-3XE-2542

Raf12 has lethal phenotype, suppressible by ER2-4XE-2171

Raf12 has lethal phenotype, suppressible by ER2-4XE-2866

Raf12 has lethal phenotype, suppressible by ER3-3XE-571

Raf12 has lethal phenotype, suppressible by ER3-3XE-979

Raf12 has lethal phenotype, suppressible by ER3-5XE-2335

Raf12 has lethal phenotype, suppressible by mtsXE-2202

Raf12 has lethal phenotype, suppressible by PTP-ERXE12N

Raf12 has lethal phenotype, suppressible by SR3-10S-297

Raf12 has lethal phenotype, suppressible by aopE-867

Raf12 has partially lethal phenotype, suppressible by rlSem/rl+

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Src42ASu(Raf)1-1

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)43B1

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)41

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)61

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)71

Raf12 has lethal | pupal stage | heat sensitive phenotype, suppressible by Su(phl)91

NOT suppressed by
Statement
Reference

Raf12 has lethal phenotype, non-suppressible by EK2-5[+]/EK2-5unspecified

Raf12 has lethal phenotype, non-suppressible by EK2-7[+]/EK2-7unspecified

Raf12 has lethal phenotype, non-suppressible by SK2-3unspecified/SK2-3[+]

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

phl[+], Raf12, Ras85D[+], Ras85Dunspecified is a suppressor | partially of small body | pupal stage phenotype of Nf1E2

rl[+], rl1, phl[+], Raf12 is a suppressor | partially of small body | pupal stage phenotype of Nf1E2

Raf12 is a suppressor of melanotic mass phenotype phenotype of hopTum

NOT Suppressor of
Statement
Reference

Ras64Bunspecified, phl[+], Ras64B[+], Raf12 is a non-suppressor of small body | pupal stage phenotype of Nf1E2

Raf12 is a non-suppressor of hyperplasia | third instar larval stage phenotype of hopTum

Raf12 is a non-suppressor of visible phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

Other
Statement
Reference

EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal | dominant phenotype

EY2-8EP1160, Scer\GAL4[-], Raf12 has lethal phenotype

S2J1, phl[+]/Raf12 has lethal phenotype

Phenotype Manifest In
Enhanced by
Statement
Reference

Raf12 has eye phenotype, enhanceable by R[+]/Rap1E-814

Raf12 has eye phenotype, enhanceable by PDZ-GEF[+]/PDZ-GEFE-1102

Raf12 has eye phenotype, enhanceable by rl+/rlE-80

Raf12 has eye phenotype, enhanceable by eyaG130

Raf12 has eye phenotype, enhanceable by EY2-5AH425

Raf12 has eye phenotype, enhanceable by kisunspecified/kis[+]

Raf12 has eye phenotype, enhanceable by SKojak

Raf12 has photoreceptor cell R7 phenotype, enhanceable by SKojak

Raf12 has eye phenotype, enhanceable by spen14O1

Raf12 has eye phenotype, enhanceable by phyl1

Raf12 has eye phenotype, enhanceable by phyl2

Raf12 has eye phenotype, enhanceable by ER3-5XE-2335

Raf12 has eye phenotype, enhanceable by ksrS-627

Raf12 has eye phenotype, enhanceable by ksrXS-337

Raf12 has eye phenotype, enhanceable by mamS-639

Raf12 has eye phenotype, enhanceable by mamXS-2295

Raf12 has eye phenotype, enhanceable by rlS-135

Raf12 has eye phenotype, enhanceable by rlXS-324

Raf12 has eye phenotype, enhanceable by SR3-6S-122

Raf12 has eye phenotype, enhanceable by SR3-6XS-328

Raf12 has eye phenotype, enhanceable by ER2-7AXE-2684

Raf12 has eye phenotype, enhanceable by ER2-7BXE-2337

Raf12 has eye phenotype, enhanceable by ER2-7BXE-2457

Raf12 has eye phenotype, enhanceable by ER3-4XE-2178

Raf12 has eye phenotype, enhanceable by hhXE-2134

Raf12 has eye phenotype, enhanceable by hhXE-3161

Raf12 has eye phenotype, enhanceable by ER3-2XE-2551

Raf12 has eye phenotype, enhanceable by SR3-2S-432

Raf12 has eye phenotype, enhanceable by SR3-2XS-355

Raf12 has eye phenotype, enhanceable by Taf6XS-922

Raf12 has eye phenotype, enhanceable by mtsXE-2258

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl1

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl2

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl3

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl4

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl5

Raf12 has photoreceptor cell R1 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R2 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R3 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R4 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R5 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R6 phenotype, enhanceable by phyl6

Raf12 has photoreceptor cell R7 phenotype, enhanceable by phyl6

Raf12 has eye phenotype, enhanceable by pnt07825Δ78

NOT Enhanced by
Statement
Reference

Raf12 has phenotype, non-enhanceable by retnB142

Raf12 has phenotype, non-enhanceable by glDZ1053

Raf12 has phenotype, non-enhanceable by glER1085

Raf12 has phenotype, non-enhanceable by stgD115

Raf12 has phenotype, non-enhanceable by stgDD937

Raf12 has phenotype, non-enhanceable by EY2-5DW1080

Raf12 has phenotype, non-enhanceable by EY3-3xBE183

Raf12 has phenotype, non-enhanceable by Su(Tpl)DI998

Raf12 has phenotype, non-enhanceable by Su(Tpl)EZ1217

Raf12 has phenotype, non-enhanceable by lilliDE884

Raf12 has phenotype, non-enhanceable by lilliDR999

Raf12 has phenotype, non-enhanceable by Taf4AG456

Suppressed by
Statement
Reference

Raf12 has eye phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

Raf12 has photoreceptor cell phenotype, suppressible | partially by Bap170hfl1/Bap170[+]

Raf12 has rhabdomere phenotype, suppressible by S6kIID1/S6kII[+]

Raf12 has ommatidium phenotype, suppressible by S6kIID1/S6kII[+]

Raf12 has interommatidial bristle phenotype, suppressible by S6kIID1/S6kII[+]

Raf12 has eye phenotype, suppressible by Taf4I53

Raf12 has eye phenotype, suppressible by PTP-ERXE-3022

Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-3022

Raf12 has eye phenotype, suppressible by PTP-ER[-]

Raf12 has ommatidium phenotype, suppressible by PTP-ER[-]

Raf12 has photoreceptor cell phenotype, suppressible by PTP-ER[-]

Raf12 has eye phenotype, suppressible by PTP-ERXE-2776

Raf12 has ommatidium phenotype, suppressible by PTP-ERXE-2776

Raf12 has eye phenotype, suppressible by ER2-1E-203

Raf12 has eye phenotype, suppressible by ER2-1E-33

Raf12 has eye phenotype, suppressible by ER2-3XE-2542

Raf12 has eye phenotype, suppressible by ER2-4XE-2171

Raf12 has eye phenotype, suppressible by ER2-4XE-2866

Raf12 has eye phenotype, suppressible by ER3-3XE-571

Raf12 has eye phenotype, suppressible by ER3-3XE-979

Raf12 has eye phenotype, suppressible | partially by mtsXE-2202

Raf12 has eye phenotype, suppressible by PTP-ERXE12N

Raf12 has eye phenotype, suppressible by SR3-10S-297

Raf12 has eye phenotype, suppressible by ER2-3XE-2213

Raf12 has eye phenotype, suppressible by ER3-5XE-2617

Raf12 has eye phenotype, suppressible by mtsXE-2258

NOT suppressed by
Statement
Reference

Raf12 has phenotype, non-suppressible by EY2-5DW1080

Raf12 has phenotype, non-suppressible by EY3-3xBE183

Raf12 has phenotype, non-suppressible by Su(Tpl)DI998

Raf12 has phenotype, non-suppressible by Su(Tpl)EZ1217

Raf12 has phenotype, non-suppressible by lilliDE884

Raf12 has phenotype, non-suppressible by lilliDR999

Raf12 has phenotype, non-suppressible by Taf4AG456

Raf12 has phenotype, non-suppressible by retnB142

Raf12 has phenotype, non-suppressible by glDZ1053

Raf12 has phenotype, non-suppressible by glER1085

Raf12 has phenotype, non-suppressible by stgD115

Raf12 has phenotype, non-suppressible by stgDD937

Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Raf12 is a suppressor of lamellocyte | supernumerary phenotype of hopTum

Raf12 is a suppressor of melanotic mass phenotype of hopTum

Raf12 is a suppressor of eye phenotype of Ras85DV12.sev

NOT Suppressor of
Statement
Reference

Raf12 is a non-suppressor of thorax phenotype of PvrdsRNA.UAS, Scer\GAL4pnr-MD237

Raf12 is a non-suppressor of hemocyte | supernumerary phenotype of hopTum

Raf12 is a non-suppressor of embryonic/larval lymph gland phenotype of hopTum

Raf12 is a non-suppressor of eye phenotype of CanBGMR.PS, Pp2B-14Dact.GMR

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The rough eye phenotype seen in phl12/Y flies is dominantly enhanced by RE-814, PDZ-GEFE-1102 and rlE-80.

Bap170hfl1/+ significantly suppresses the rough eye phenotype and loss of photoreceptor cells seen in phl12 flies at 18[o]C.

The lethality caused by phl12 at 25[o]C is partially rescued by Bap170hfl1/+.

Expression of S6kIIScer\UAS.T:Hsap\MYC, under the control of Scer\GAL4GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a phl12/+ background than when expressed in a wild-type background.

The eye roughness and decreased rhabdomere phenotypes seen in phl12 flies are suppressed in phl12, S6kIID1/Y flies. The male temperature sensitive lethality of phl12/Y flies is suppressed in phl12/Y, S6kIID1/Y flies.

When phl12 is added to slikScer\UAS.cHa, Scer\GAL4ptc-559.1 animals the overgrowth phenotype is partially suppressed.

At 28oC, less than 3% lamellocytes are seen in phl12 hopTum larvae. Plasmatocytes with abnormal morphology are occasionally seen in these animals; some blood cells are larger than the plasmatocytes normally seen in wild-type larvae, while others have processes or an elongated morphology. The overproliferation of blood cells seen in hopTum larvae is not suppressed by phl12. phl12 hopTum larvae have reduced melanotic tumour formation compared to hopTum larvae, but the lymph glands are still overgrown.

The loss of photoreceptors seen in phl12/Y flies is further enhanced by the addition of lilli3E8 clones in the eye.

Heterozygous PTP-ERXE-3022, PTP-ERXE-2776 as well as deficiency alleles of PTP-ER suppress the rough eyes and loss of photoreceptor cells seen in hemizygous phl12.

The hemizygous phenotype is enhanced by SKojak. phl12/Y ; SKojak/+ flies have no R7 photoreceptor cells. Flies doubly mutant for phl12 and SIIN have no R7 photoreceptor cells.

The lethality of phl12 is dominantly enhanced by sag13L, sag32-3, sagA4-2 or sag16-2.

Synthetic lethal with alleles of cnk.

Eye phenotype is enhanced by mutations in phyl and spen. Synthetic lethal in combination with alleles of spen, S, rl, Hsp83 and 14-3-3ε.

Phenotype can be slightly rescued (60-70% ommatidia have wild-type complement of photoreceptor cells) by JraS82D.T92D.T107D.hs.sev.

The phenotype is dominantly enhanced by phyl mutants.

Reduced viability is dominantly suppressed by rlSem. Surviving flies have a reduced number of photoreceptors.

One copy of Su(phl)6, Su(phl)43B, Su(phl)9, Src42A, Su(phl)7, Su(phl)4 and Dsor1Su34B can rescue lethality at the restrictive temperature.

Acts as a dominant suppressor of the activated Ras85DV12.sev. Halving the gene dosage of pnt enhances both the rough eye and missing photoreceptor phenotypes of Dsor1XS520 and phl12.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Described in Kramers, Mutation Research 107:187.

Allele designation of hypomorph based on observation that homozygotes die at pupal-adult interface as but heterozygotes with Df(1)64c18 die as pupae. Isolation described in Kramers et al., Mut. Res., 107: 187.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (44)