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General Information
Symbol
Dmel\pip1
Species
D. melanogaster
Name
pipe1
FlyBase ID
FBal0013836
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pip386, pipe386, pipe1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T19300115A

Amino acid change:

V133D | pip-PA

Reported amino acid change:

V123D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Difference between reported and FlyBase amino acid change due to authors use of GenBank record gi:7293803 which has since been replaced by a new version gi:28380469.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation is within the fourth pip-PA (Pipe-ST2) specific exon.

Amino acid replacement: V123D.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pip1 homozygous females produce dorsalised embryos.

All embryos from females carrying pip1 are completely dorsalized.

Eggs from pip1/pip2 mothers do not have apparent vitelline membrane defects.

Dorsalised embryos. dec-1-marked clones restricted to the dorsal side of the follicular epithelium produce normal embryos with no defects in dorsoventral patterning. Clones on the dorsal side produce local dorsalisation of the embryo. Clones in the ventral anterior region yield embryos exhibiting anterior dorsalisation, clones in the ventral posterior produce result in dorsalisation of the embryo at the posterior.

Embryos derived from pip1/pip2 females are dorsalised, showing symmetric folds during gastrulation and forming a tube of dorsal cuticle. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL425 show strong ventralisation, producing cuticles with rings of ventral denticle material. The orientation of gastrulation in these embryos is variable and they show muscle movement. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL4241 show gastrulation movements that are inverted with respect to the intrinsic orientation of the eggshell. The most ventral structures differentiated by most of these embryos are Filzkorper, although some embryos develop ventral denticle material. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL4DE1 form a ventral furrow invagination only at the posterior end. These embryos form rings of denticles at the posterior end.

Mutant embryos respond to injected purified polarizing activity (spz), and produce polarizing activity themselves.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

pip1 has embryo | dorsal phenotype, suppressible by gdWT.cHa

Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of eaScer\UAS.P\T.T:Ivir\HA1 and snkScer\UAS.P\T.T:Avic\GFP-m6 using Scer\GAL4unspecified in a pip1/pip3 background results in the production of dorsalized embryos.

pip1/pip1 and pip1/pip2 mutants carrying germ-line clones of ik21 mothers produce dorsalised embryos, in the same manner as homozygous pip1 or pip1/pip2 mutants.

Embryos derived from Spn27Aex32/Df(2L)BSC7 ; pip1/pip2 females are completely dorsalised.

Perivitelline fluid from embryos laid by spz4/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by spzD1-RPQ/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by Tlrv13 spz4/Tlrv4 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD3 spz4/ea1 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD1 spz2/ea4 spz4 females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by Tlrv13/Tlrv19 females shows polarising activity when injected into the perivitelline space of embryos derived from pip1/pip2 females.

Double mutant combinations with eaD1 are strongly dorsalizing.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Strong mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (32)