FB2025_01 , released February 20, 2025
Allele: Dmel\png3318
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General Information
Symbol
Dmel\png3318
Species
D. melanogaster
Name
FlyBase ID
FBal0013904
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
png12-3318
Key Links
Genomic Maps

Allele class
Nature of the Allele
Allele class
Progenitor genotype
Cytology
Description

Amino acid replacement: G17S.

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Nucleotide change:

G1357485A

Amino acid change:

G17S | png-PA

Reported amino acid change:
Comment:

site of nucleic acid difference inferred by FlyBase based on stated amino acid change

Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Both unfertilized eggs and blastoderm stage embryos from png2 mothers have enlarged nuclei. The affected embryos have many fewer nuclei than wild-type controls.

png5/png5 females produce two classes of embryos 30-55% are "multinucleate" (indicating that some mitotic division has occurred), containing more than 5 giant polyploid nuclei. The remaining embryos contain up to 5 giant polyploid nuclei (indicating a lack of mitotic divisions). Homozygous females produce 5-9% of "multinucleate" embryos.

The four meiotic products present in unfertilised eggs laid by homozygous females overreplicate and fuse to form a single polyploid nucleus. Overreplication of the polar bodies is seen in fertilised embryos laid by homozygous females. The zygotic nuclei of these embryos appear to undergo normal S-M cycles initially, but linkage between S phase and M-phase breaks down, resulting in the zygotic nuclei becoming polyploid.

Unfertilised eggs from a homozygous mother initiate DNA replication inappropriately and have a single giant polyploid nuclei, fertilised eggs exhibit DNA replication that is partially uncoupled from nuclear division resulting in 6-16 giant polyploid nuclei. Trans-heterozygotes with the single nuclei alleles, png1, png3 and png5, produce embryos with multiple polyploid nuclei. png2 plu3 double homozygous mutant mothers produce embryos with six or less giant polyploid nuclei.

Embryonic defects detectable during preblastoderm stages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

png1058/png3318 has phenotype, non-enhanceable by Df(1)bi-D3

NOT suppressed by
Statement
Reference

png1058/png3318 has phenotype, non-suppressible by Df(1)bi-D3

Additional Comments
Genetic Interactions
Statement
Reference

Embryos derived from png2 homozygous females that are also carrying multiple copies of CycB+t10 show a striking increase in the number of nuclei in each embryo compared to embryos derived from png2 females, as well as an increase in the number of embryos with multiple nuclei. The polar bodies contain condensed chromosomes with a normal rosette arrangement. The chromosomes of the zygotic nuclei are condensed and mitotic figures are visible. However, all the embryos contain polyploid nuclei and none survive past embryogenesis.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Residual png activity permits a transient link of DNA replication to mitosis.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (11)