FlyBase Allele Report: Dmel\png[3318]

General Information

Symbol

Dmel\png³³¹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0013904

Feature type

allele

Associated gene

Dmel\png

Associated Insertion(s)

Carried in Construct

Also Known As

png^12-3318

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: G17S.

(Fenger et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:1,357,485..1,357,485 [-]

Nucleotide change:

G1357485A

Amino acid change:

G17S | png-PA

Reported amino acid change:

G17S|FBrf0129806

Comment:

site of nucleic acid difference inferred by FlyBase based on stated amino acid change

(Fenger et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | embryonic stage

(Shamanski and Orr-Weaver, 1991)

abnormal mitotic cell cycle | embryonic stage

(Elfring et al., 1997)

abnormal mitotic cell cycle | maternal effect (with png¹⁰⁵⁸)

(Lee et al., 2001)

female sterile

(Lindsley and Zimm, 1992)

lethal | maternal effect

(Shamanski and Orr-Weaver, 1991, Mohler and Carroll, 1984)

Phenotype Manifest In

nuclear chromosome

(Shamanski and Orr-Weaver, 1991)

nucleus

(Shamanski and Orr-Weaver, 1991)

nucleus & unfertilized egg

(Lee et al., 2005)

nucleus | blastoderm stage | maternal effect

(Lee et al., 2005)

nucleus | embryonic stage

(Elfring et al., 1997)

nucleus | maternal effect

(Elfring et al., 1997)

polar body nucleus

(Elfring et al., 1997)

unfertilized egg

(Elfring et al., 1997)

Detailed Description

Statement

Reference

Both unfertilized eggs and blastoderm stage embryos from png² mothers have enlarged nuclei. The affected embryos have many fewer nuclei than wild-type controls.

(Lee et al., 2005)

png⁵/png⁵ females produce two classes of embryos 30-55% are "multinucleate" (indicating that some mitotic division has occurred), containing more than 5 giant polyploid nuclei. The remaining embryos contain up to 5 giant polyploid nuclei (indicating a lack of mitotic divisions). Homozygous females produce 5-9% of "multinucleate" embryos.

(Lee et al., 2001)

The four meiotic products present in unfertilised eggs laid by homozygous females overreplicate and fuse to form a single polyploid nucleus. Overreplication of the polar bodies is seen in fertilised embryos laid by homozygous females. The zygotic nuclei of these embryos appear to undergo normal S-M cycles initially, but linkage between S phase and M-phase breaks down, resulting in the zygotic nuclei becoming polyploid.

(Elfring et al., 1997)

Unfertilised eggs from a homozygous mother initiate DNA replication inappropriately and have a single giant polyploid nuclei, fertilised eggs exhibit DNA replication that is partially uncoupled from nuclear division resulting in 6-16 giant polyploid nuclei. Trans-heterozygotes with the single nuclei alleles, png¹, png³ and png⁵, produce embryos with multiple polyploid nuclei. png² plu³ double homozygous mutant mothers produce embryos with six or less giant polyploid nuclei.

(Shamanski and Orr-Weaver, 1991)

Embryonic defects detectable during preblastoderm stages.

(Mohler and Carroll, 1984)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycA^hs.PK

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycB[+]/CycB²

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)173/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2R)44CE

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)59AB/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2R)59AD

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)M60E/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)X58-7/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycE⁰⁵²⁰⁶

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)nap9/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)Ly/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)W4/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)fz-GF3b/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)st-f13/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(3R)C4

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)Scr/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)crb87-4/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2L)C144/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2L)E55

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2L)dp-79b

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2L)r10/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)e-BS2/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)pk78s/+

(Lee et al., 2001)

NOT Enhanced by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by CycA⁵

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by CycB3²

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by Df(3L)HR119

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF3h^k09003

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF4E1⁰⁷²³⁸

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF4A^k01501

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by RpL30^k00308

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by AspRS^k04508

(Lee et al., 2001)

Suppressed by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by CycB^hs.PK

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)B5/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)PC4/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)Px2/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)cn9/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(3L)66C-G28/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(3R)Cha7/+

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by +/Df(3R)ry615

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B^j6E7

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by dap⁰⁴⁴⁵⁴

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by eEF5⁰¹²⁹⁶

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by eEF5¹⁰⁵³⁰

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by mts[+]/mts^k09822

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by mts^s5286

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2L)osp29/+

(Lee et al., 2001)

png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible by CycB^+t10

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by CycB3^+t9

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/CycB^D46.UASp

(Lee et al., 2001)

NOT suppressed by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by AspRS^k04508

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by CycB3²

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by Df(3L)HR119

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF3h^k09003

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF4E1⁰⁷²³⁸

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF4A^k01501

(Lee et al., 2001)

png¹⁰⁵⁸/png³³¹⁸ has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by RpL30^k00308

(Lee et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has phenotype, non-enhanceable by Df(1)bi-D3

(Lee et al., 2001)

NOT suppressed by

Statement

Reference

png¹⁰⁵⁸/png³³¹⁸ has phenotype, non-suppressible by Df(1)bi-D3

(Lee et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from png² homozygous females that are also carrying multiple copies of CycB^+t10 show a striking increase in the number of nuclei in each embryo compared to embryos derived from png² females, as well as an increase in the number of embryos with multiple nuclei. The polar bodies contain condensed chromosomes with a normal rosette arrangement. The chromosomes of the zygotic nuclei are condensed and mitotic figures are visible. However, all the embryos contain polyploid nuclei and none survive past embryogenesis.

(Lee et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

png^171a

(Fenger et al., 2000)

png¹⁷²

(Fenger et al., 2000)

png²⁴⁶

(Fenger et al., 2000)

png⁵⁰

(Fenger et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38436

y¹ png³³¹⁸ w^*/FM0

Notes on Origin

Discoverer

Comments

Residual png activity permits a transient link of DNA replication to mitosis.

(Shamanski and Orr-Weaver, 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

fs(1)12-3318

(Lindsley and Zimm, 1992)

fs(1)M2^12-3318

(Mohler and Carroll, 1984)

png^12-3318

(Tadros et al., 2007, Tadros et al., 2003, Lee et al., 2001, Fenger et al., 2000, Elfring et al., 1997, Shamanski and Orr-Weaver, 1991)

png²

(Orr-Weaver, 2008.5.5)

png³³¹⁸

(Lerit and Gavis, 2011, Orr-Weaver, 2008.5.5, Tadros et al., 2007, Lee et al., 2005, Lee et al., 2001, Fenger et al., 2000, Elfring et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (11)

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