Amino acid replacement: G17S.
G1357485A
G17S | png-PA
G17S|FBrf0129806
site of nucleic acid difference inferred by FlyBase based on stated amino acid change
nucleus & unfertilized egg
Both unfertilized eggs and blastoderm stage embryos from png2 mothers have enlarged nuclei. The affected embryos have many fewer nuclei than wild-type controls.
png5/png5 females produce two classes of embryos 30-55% are "multinucleate" (indicating that some mitotic division has occurred), containing more than 5 giant polyploid nuclei. The remaining embryos contain up to 5 giant polyploid nuclei (indicating a lack of mitotic divisions). Homozygous females produce 5-9% of "multinucleate" embryos.
The four meiotic products present in unfertilised eggs laid by homozygous females overreplicate and fuse to form a single polyploid nucleus. Overreplication of the polar bodies is seen in fertilised embryos laid by homozygous females. The zygotic nuclei of these embryos appear to undergo normal S-M cycles initially, but linkage between S phase and M-phase breaks down, resulting in the zygotic nuclei becoming polyploid.
Unfertilised eggs from a homozygous mother initiate DNA replication inappropriately and have a single giant polyploid nuclei, fertilised eggs exhibit DNA replication that is partially uncoupled from nuclear division resulting in 6-16 giant polyploid nuclei. Trans-heterozygotes with the single nuclei alleles, png1, png3 and png5, produce embryos with multiple polyploid nuclei. png2 plu3 double homozygous mutant mothers produce embryos with six or less giant polyploid nuclei.
Embryonic defects detectable during preblastoderm stages.
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycAhs.PK
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycB[+]/CycB2
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)173/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2R)44CE
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)59AB/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2R)59AD
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)M60E/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)X58-7/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by CycE05206
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)nap9/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)Ly/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)W4/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)fz-GF3b/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3L)st-f13/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(3R)C4
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)Scr/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)crb87-4/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2L)C144/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2L)E55
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by +/Df(2L)dp-79b
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2L)r10/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(3R)e-BS2/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, enhanceable by Df(2R)pk78s/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by CycA5
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by CycB32
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by Df(3L)HR119
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF3hk09003
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF4E107238
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by eIF4Ak01501
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by RpL30k00308
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-enhanceable by AspRSk04508
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by CycBhs.PK
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)B5/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)PC4/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)Px2/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2R)cn9/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(3L)66C-G28/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(3R)Cha7/+
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by +/Df(3R)ry615
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87Bj6E7
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by dap04454
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by eEF501296
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by eEF510530
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by mts[+]/mtsk09822
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by mtss5286
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Df(2L)osp29/+
png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible by CycB+t10
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by CycB3+t9
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, suppressible | partially by Scer\GAL4VP16.nanos.UTR/CycBD46.UASp
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by AspRSk04508
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by CycB32
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by Df(3L)HR119
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF3hk09003
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF4E107238
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by eIF4Ak01501
png1058/png3318 has abnormal mitotic cell cycle | maternal effect phenotype, non-suppressible by RpL30k00308
png1058/png3318 has phenotype, non-enhanceable by Df(1)bi-D3
png1058/png3318 has phenotype, non-suppressible by Df(1)bi-D3
Embryos derived from png2 homozygous females that are also carrying multiple copies of CycB+t10 show a striking increase in the number of nuclei in each embryo compared to embryos derived from png2 females, as well as an increase in the number of embryos with multiple nuclei. The polar bodies contain condensed chromosomes with a normal rosette arrangement. The chromosomes of the zygotic nuclei are condensed and mitotic figures are visible. However, all the embryos contain polyploid nuclei and none survive past embryogenesis.
Residual png activity permits a transient link of DNA replication to mitosis.