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General Information
Symbol
Dmel\Pp1-87B1
Species
D. melanogaster
Name
FlyBase ID
FBal0013933
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
PP1α87B1, Su(var)3-601, Su(var)3-61, Su-var(3)601
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G12425031A

Amino acid change:

G220S | Pp1-87B-PA

Reported amino acid change:

G220S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Amino acid replacement: G220S.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pp1-87B1 heterozygous embryos appear wild-type and hatch normally.

Pp1-87B1/Pp1-87B87Bg-3 animals die during pupariation, and until pupariation, do not lag behind in development compared to their control siblings.

Homozygotes and heterozygotes show no muscle detachment or indirect flight muscle phenotypes.

Suppresses the position effect variegation at the w locus caused by In(1)wm4 and suppresses the position effect variegation at the bw locus caused by In(2R)bwVDe2 and Byron. Also suppresses the position effect variegation of w seen in the P{wAR}41A insertion.

Strong suppressor levels of position effect vareigation and normal mitosis in neuroblasts or ganglion mother cells.

Dominant suppressor of position effect variegation at the w locus caused by In(1)wm4h.

shows some reduction in viability in trans heterozygotes with some alleles of l(3)87Bg; in one case a clear maternal effect is noted.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Pp1-87B1 is an enhancer of visible phenotype of RetMEN2B.GMR

Pp1-87B1 is an enhancer of visible phenotype of RetGMR.PR

Pp1-87B[+]/Pp1-87B1 is an enhancer of visible phenotype of Scer\GAL4vg.PM, putUAS.cMa

NOT Enhancer of
Statement
Reference

Pp1-87B1 is a non-enhancer of visible phenotype of RetMEN2A.GMR

Suppressor of
Statement
Reference

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of visible | homeotic phenotype of trxE2/trx1

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of visible | homeotic phenotype of trxB11/trx1

NOT Suppressor of
Statement
Reference

Pp1-87B1 is a non-suppressor of visible phenotype of RetMEN2A.GMR

Other
Phenotype Manifest In
Enhancer of
Statement
Reference

Pp1-87B1 is an enhancer of eye phenotype of RetMEN2B.GMR

Pp1-87B1 is an enhancer of eye phenotype of RetGMR.PR

Pp1-87B[+]/Pp1-87B1 is an enhancer of wing phenotype of Scer\GAL4vg.PM, putUAS.cMa

NOT Enhancer of
Statement
Reference

Pp1-87B1 is a non-enhancer of eye phenotype of RetMEN2A.GMR

Suppressor of
Statement
Reference

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of haltere | ectopic phenotype of trxB11/trx1

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of wing phenotype of trxE2/trx1

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of haltere | ectopic phenotype of trxE2/trx1

Pp1-87B[+]/Pp1-87B1 is a suppressor | partially of wing phenotype of trxB11/trx1

NOT Suppressor of
Statement
Reference

Pp1-87B1 is a non-suppressor of eye phenotype of RetMEN2A.GMR

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Embryos laid by flies heterozygous for mars1 and Pp1-87B1 show a significantly reduced hatch ratio.

Pp1-87B1/Pp1-87B87Bg-3 Pp1α-96A2/Pp1α-96A2 double mutant animals die slightly earlier than Pp1-87B1/Pp1-87B87Bg-3 single mutants and pupariate 1-2 days later than their siblings.

Expression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 in Pp1-87B1/+ wings results in severely crumpled and reduced wings.

The modest wing overgrowth phenotype caused by expression of putScer\UAS.cMa under the control of Scer\GAL4vg.PM is enhanced by Pp1-87B1/+.

Pp1-87B1 dominantly suppresses the transformation of wing to haltere seen in trx1/trxE2 flies; 31% show strong transformation, 39% show weak transformation and 30% show no transformation.

Pp1-87B1 dominantly suppresses the transformation of wing to haltere seen in trx1/trxB11 flies; 27% show strong transformation, 24% show weak transformation and 49% show no transformation.

Flies expressing NiPp1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4vg.PM which are also carrying Pp1-87B1 have crumpled wings.

The effect of loss of one copy of Pp1-87B1 cannot be rescued by P element mediated transformation with Su(var)3-7.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The suppressor phenotype of Pp1-87B1 on In(1)wm4h is rescued by Pp1-87B+t5.8. The lethality of Pp1-87B1 is rescued by Pp1-87B+t5.8.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Not suppressed by zip alleles.

Isolated as a suppressor of position effect variegation at the w locus in flies carrying In(1)wm4h, and a dominant enhancer of position effect variegation, E(var)c1011.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (29)