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General Information
Symbol
Dmel\Pp1-87B87Bg-3
Species
D. melanogaster
Name
FlyBase ID
FBal0013938
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
PP1α87B87Bg-3, PP1α87Be211, ck19e211
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Deletion with a breakpoint 165bp upstream of the ATG initiation site. This removes the TATA box and other upstream sequences. Extent of deletion unknown.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

There may be a DNA rearrangement at the 5' end of the Pp1-87B87Bg-3 gene, however no lesion was detected cytologically.

Sequence 165bp and more 5' upstream of the ATG initiation site diverges from the wild-type sequence due to a deletion of DNA. This removes the TATA box.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eye morphogenesis is disrupted and eye size is reduced in animals containing eyes that are almost completely homozygous for Pp1-87B87Bg-3. In addition, mutant photoreceptor cells, cone cells and undifferentiated cells are mislocalised in the optic stalk of these animals.

Pp1-87B1/Pp1-87B87Bg-3 animals die during pupariation, and until pupariation, do not lag behind in development compared to their control siblings.

Pp1-87B87Bg-3/Pp1-87B87Bg-6 larvae show axon clumping at the lamina and laminal breaks with ~50% of R2-R5 axons being mistargeted to the medulla.

Squash preparations of Pp1-87B87Bg-3/Df(3R)kar-H10 third larval instar brains contain fewer cells per microscope field than normal and a significantly higher proportion of cells in mitosis than in wild type. Overcondensed chromosomes are seen.

Weak suppressor levels of position effect vareigation and a high frequency of aberrant mitosis in neuroblasts or ganglion mother cells. Metaphase chromosomes and anaphase chromatids are over condensed.

Larvae die either just before or just after pupariation. Third instar larval imaginal discs are underdeveloped, indicating failure of diploid cell proliferation. Ganglia are smaller than those of wild-type larvae, with significantly fewer cells per microscope field in squash preparations, suggesting cell division is impaired in the ganglia. The proportion of cells in mitosis in the ganglia is significantly higher than in wild type. This is due to a large number of mitotic cells showing abnormalities, such as overcondensed or extra chromosomes. These cells are probably delayed in progress through mitosis. Spindle organisation is also defective, showing unusually little space between almost parallel bundles of spindle microtubules at metaphase.

L2/L3 larval lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Pp1-87B87Bg-3 does not show any discernible mutant phenotype with pucA251.1F3.

Pp1-87B1/Pp1-87B87Bg-3 Pp1α-96A2/Pp1α-96A2 double mutant animals die slightly earlier than Pp1-87B1/Pp1-87B87Bg-3 single mutants and pupariate 1-2 days later than their siblings.

Expression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 in Pp1-87B87Bg-3/+ wings results in severely crumpled and reduced wings.

Expression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4arm.PS is synthetic lethal in a heterozygous Pp1-87B87Bg-3 mutant background.

The synthetic lethality resulting from the overexpression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4arm.PS in a heterozygous Pp1-87B87Bg-3 mutant background can be rescued by the co-expression of Pp1-87BScer\UAS.T:Ivir\HA1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Not suppressed by zip alleles.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (16)