1.1kb insertion after amino acid 45 of the paired-domain.
N-terminal insertion of a 1.1kb fragment of repetitive DNA.
When gsbprd.PX is added to Df(2L)Prl/prd4 animals, males survive to adulthood but are sterile and have smaller or no accessory glands. The addition of prdmf5 to prd4/Df(2L)Prl animals that have been partially rescued by gsbprd.PX, fully rescues the male sterility phenotype. When CycEScer\UAS.cLa or dmScer\UAS.cZa (driven by Scer\GAL4mf9) is added to Df(2L)Prl/prd4, (partially rescued by a weakly expressing prdmf9 line), the accessory gland phenotype is reduced, and male fertility is restored. When CycEScer\UAS.cLa or (driven by Scer\GAL4prd.3.1) is added to Df(2L)Prl/prd4, however, no rescue is seen. When Df(3L)H99, Df(3L)th102 or Pi3K92EScer\UAS.T:Hsap\MYC is added to Df(2L)Prl/prd4 animals (already partially suppressed by the addition of prdmf9) no suppression is seen in the accessory gland or make sterility phenotypes.
The addition of gsbprd.PX to prd4/Df(2L)Prl flies produces male flies that are sterile. These males exhibit normal copulatory behaviour. The testes, the ejaculatory duct, and the ejaculatory bulb are without obvious morphological defects. In addition, a normal amount of sperm is transferred to females during copulation. However, accessory glands are completely absent in these flies.
prdΔPBC or prd+mXa rescues the larval pair rule phenotype and embryonic lethality seen in prd4/Df(2L)Prl animals; however rescue with prdΔPBC leads to a distorted segmentation phenotype in adult cuticles. prdΔPBC rescues accessory gland development and male fertility (not fully) to a similar extent as prd+mXa in prd4/Df(2L)Prl; however rescue with prdΔPBC leads to significantly reduced fecundity (fewer progeny) compared to rescue with prd+mXa. prdwt fails to rescue accessory gland development in prd4/Df(2L)Prl males. prd4/Df(2L)Prl males rescued with prdΔPBC show lower mating success compared to those rescued with prd+mXa, though they show no significant difference in climbing ability. Females mated to prd4/Df(2L)Prl males rescued with prdΔPBC (even two copies) behave like virgins, with low egg-laying, high receptivity and low rejection; mutant males rescued with prd+mXa behave similar to controls.