Nucleotides 4224 to 4498 have been deleted. This produces a pum protein that lacks a RNA-binding domain.
The phenotype of extra germline stem cells that is seen in the germarium of females expressing stwlEY05697 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is suppressed if the females also carry pum9/+.
pum02003 bamΔ86/pum9 bamΔ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals.
Expression of pumScer\UAS.fl at 18oC in postmitotic neurons (Scer\GAL4elav-C155) rescues the synaptic morphology phenotypes found in a pum9/pum7 background. Both the average size and the number of type 1b boutons on muscle 12 are restored to wild-type.
Expression of pumScer\UAS.fl in muscles (Scer\GAL4Mhc.Switch.PO) in pum9/pum7 larvae reduces the number of eIF-4E aggregates by 7-fold, to 3.5 aggregates per neuromuscular junction. The level of eIF-4E is reduced by approximately 6.5-fold. In contrast, neural expression of pumScer\UAS.fl (Scer\GAL4elav-C155) in pum9/pum7 larvae does not greatly decrease the number of eIF-4E aggregates (although a 20% reduction is seen). Levels of eIF-4E are 88% of those in pum9/pum7 larvae.
The increase in 1s bouton number in pum9/pum7 mutants is fully rescued through expression of pumScer\UAS.fl in muscles (Scer\GAL4Mhc.Switch.PO), and is unaffected by expression in neurons (under the control of Scer\GAL4elav-C155).
The mEJP amplitude and quantal content phenotypes of pum9/pum7 mutants are not rescued to near wild-type levels by neuronal (Scer\GAL4elav-C155), muscle (Scer\GAL4Mhc.Switch.PO), or dual expression of pumScer\UAS.fl.
Expression of pumRBD.Scer\UAS at 18oC in postmitotic neurons (Scer\GAL4elav-C155) does not rescue the morphology phenotypes found in a pum9/pum7 background, in contrast to its ability to provide the early abdominal segmentation function.