Allele Dmel\rb¹

General Information
Symbol	Dmel\rb¹	Species	D. melanogaster
Name		FlyBase ID	FBal0014434
Feature type	allele	Associated gene	Dmel\rb
Also Known As	ruby¹

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	eye color defective (Cheli et al., 2010, Cheli et al., 2010) eye color defective \| recessive (Kretzschmar et al., 2000) short lived \| male (Simonsen et al., 2007) visible \| recessive
Phenotype Manifest In
	Malpighian tubule (Beadle, 1937) ocellus pigment granule photoreceptor cell pigment granule (Mullins et al., 2000) pigment cell (Kretzschmar et al., 2000) testis pigment cell
Detailed Description
	Statement Reference Mutant flies show a significant decrease in ommochrome and drosopterin pigment levels compared to wild type. (Harris et al., 2011) Mutant flies have reduced red pigment in the eye compared to controls. (Cheli et al., 2010, Cheli et al., 2010) Mutant males have a shortened adult life span compared to controls, with a mean life span of 31.57 days at 25^o. (Simonsen et al., 2007) Eye colour: the drosopterin (red pigment) content of rb¹ flies is reduced to 25% of wild type. Eye colour: the drosopterin (red pigment) content of g¹ rb¹ double mutant flies is reduced to 18% of wild type. Eye colour: the xanthommatin (brown pigment) content of rb¹ flies is reduced to 58% of wild type. Eye colour: the xanthommatin (brown pigment) content of g¹ rb¹ double mutant flies is reduced to 41% of wild type. (Kretzschmar et al., 2000) Examination of mutant eyes reveal a dramatic decrease in the number of electron dense pigment granules seen surrounding groups of photoreceptor cells. Examination of retinula cells from rb¹ mutants reveals normal neuronal anatomy, including normal cytoplasmic synaptic vesicles (SVs). In an assay for larval motility, rb¹ mutants performed as well as wild-type larvae. In locomotor assays testing olfactory response to propionic acid, rb¹ mutants perform significantly less well than wild-type larvae. (Mullins et al., 2000) Malpighian tubule colour: pale yellow. (Beadle, 1937) Development of pigment autonomous in eye discs transplanted into wild-type hosts. (Beadle and Ephrussi, 1936) RK1. Eye colour: clear ruby. Ocellus colour: colourless. Testis colour: colourless.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference rb¹ has eye color defective phenotype, enhanceable by aux^Scer\UAS.cHa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Cheli et al., 2010) rb¹ has eye color defective phenotype, enhanceable by blos1^ex2 (Cheli et al., 2010)
Enhancer of
Statement Reference rb¹ is an enhancer of eye color defective phenotype of blos1^ex2 (Cheli et al., 2010) rb¹ is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, aos^GD16463 (Harris et al., 2011)
Other
Statement Reference ltd¹, rb¹ has eye color defective phenotype (Silva and Mensua, 1985, Silva and Mensua, 1985)
Phenotype Manifest In
NOT suppressed by
Statement Reference rb¹ has phenotype, non-suppressible by su(Hw)² (Lindsley and Grell, 1968)
Enhancer of
Statement Reference rb¹ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, aos^GD16463 (Harris et al., 2011)
Other
Statement Reference ltd¹, rb¹ has pigment cell phenotype (Silva and Mensua, 1985, Silva and Mensua, 1985)
Additional Comments
Genetic Interactions
Statement Reference The mispatterning of the eye caused by expression of aos[GD16463] under the control of Scer\GAL4[GMR.PF] is modestly enhanced in a rb[1] mutant background. (Harris et al., 2011) The reduction in red pigment in the eye seen in rb[1] blos1[ex2] double mutants is more severe than that seen in either single mutant. (Cheli et al., 2010) Expression of aux[Scer\UAS.cHa] under the control of Scer\GAL4[GMR.PF] enhances the loss of red eye pigment that is seen in rb[1] flies. (Cheli et al., 2010) rb¹ is a complex modifier of the eye phenotype caused by expression of bchs^EP2299 under the control of Scer\GAL4^GMR.PF. (Simonsen et al., 2007) The level of pteridine eye pigments in rb¹ ; ltd¹ double mutant flies are significantly lower than that of the corresponding single mutants. (Ma et al., 2004) Eye colour: nearly white, with ltd¹. Pale eye color due to the absence of pteridines and brown pigment. (Silva and Mensua, 1985) Eye colour: white, with w^a. Eye colour: orange, with st¹. Eye colour: brownish red, with bw¹. (Mainx, 1938)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 23 )
Bloomington	88 rb¹ 4170 rb¹ cx¹ 2209 N^nd-1 rb¹ 3075 w^a N^l1N-ts2 rb¹ 1260 w^a su(fa^swb)¹ rb¹ 2210 N^nd-3 rb¹/C(1)DX, y¹ f¹ 14 br¹ w^e ec¹ rb¹ t⁴/FM1, lz⁺
Kyoto	105725 rb¹ 101172 rb¹ cx¹ 106821 w^a N^l1N-ts2 rb¹ 106742 N^nd-3 rb¹/C(1)DX, y¹ f¹ 105675 br¹ w^e ec¹ rb¹ t⁴/FM1, lz⁺
	More stocks available...
Notes on Origin
Discoverer	Bridges, 18th Oct. 1914.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	rb¹ (Harris et al., 2011, Christensen et al., 2008.6.11, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Cheli et al., 2010, Cheli et al., 2010, Popodi et al., 2010-) ruby¹ (Yuva-Aydemir et al., 2011, Simonsen et al., 2007) unnamed (Silva and Mensua, 1985)
Name Synonym
Secondary FlyBase IDs

References ( 19 )
Research paper	Harris et al., 2011, J. Cell Biol. 194(1): 77--87 Cargo sorting to lysosome-related organelles regulates siRNA-mediated gene silencing. [FBrf0214287] Yuva-Aydemir et al., 2011, J. Neurosci. 31(19): 7005--7015 Spinster Controls Dpp Signaling during Glial Migration in the Drosophila Eye. [FBrf0213705] Cheli et al., 2010, Hum. Mol. Genet. 19(5): 861--878 Genetic modifiers of abnormal organelle biogenesis in a Drosophila model of BLOC-1 deficiency. [FBrf0209903] Simonsen et al., 2007, Genetics 176(2): 1283--1297 Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles. [FBrf0201586] Ma et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(32): 11652--11657 Lightoid and Claret: a rab GTPase and its putative guanine nucleotide exchange factor in biogenesis of Drosophila eye pigment granules. [FBrf0180621] Kretzschmar et al., 2000, Genetics 155(1): 213--223 Defective pigment granule biogenesis and aberrant behavior caused by mutations in the Drosophila AP-3 adaptin gene ruby. [FBrf0127175] Mullins et al., 2000, Mol. Gen. Genet. 263(6): 1003--1014 Distinct requirements for the AP-3 adaptor complex in pigment granule and synaptic vesicle biogenesis in Drosophila melanogaster. [FBrf0129983] Tearle, 1991, Genet. Res. (Camb.) 57: 257--266 Tissue specific effects of ommochrome pathway mutations in Drosophila melanogaster. [FBrf0054090] Silva and Mensua, 1985, D. I. S. 61: 156 Synergistic effects between eye-color genes of Drosophila melanogaster. [FBrf0042242] Mainx, 1938, Z. indukt. Abstamm.- u. VererbLehre 75: 256--276 Analyse der Genwirkung durch Faktorenkombination. Versuche mit den Augenfarbenfaktoren von Drosophila melanogaster. [FBrf0004454] Beadle, 1937, Genetics 22: 587--611 Development of eye colors in Drosophila: fat bodies and Malpighian tubes as sources of diffusible substances. [FBrf0003911] Beadle and Ephrussi, 1936, Genetics 21: 225--247 The differentiation of eye pigments in Drosophila as studied by transplantation. [FBrf0003530]
Supplementary material	Cheli et al., 2010, Hum. Mol. Genet. 19(5): Supplementary Data. [FBrf0210495]
Review	Boehm and Bonifacino, 2002, Gene 286(2): 175--186 Genetic analyses of adaptin function from yeast to mammals. [FBrf0148996]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621] Christensen et al., 2008.6.11, Isolation and characterization of Df(1)BSC532. Isolation and characterization of Df(1)BSC532. [FBrf0204973] Christensen et al., 2008.9.3, Isolation and characterization of Df(1)BSC580. Isolation and characterization of Df(1)BSC580. [FBrf0205596] Christensen et al., 2008.12.28, Isolation and characterization of Df(1)BSC657. Isolation and characterization of Df(1)BSC657. [FBrf0206413]
Book	Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044]

Allele Dmel\rb1

Allele Dmel\rb¹