The increased concentration of circulating hemocytes characteristic for Graf1/Y mutant third instar larvae is partially suppressible by combination with a single copy of rl1; combination with rl1/rl10 reduces the hemocyte levels in Graf1/Y larvae to below wild-type levels, comparable to those seen in rl1/rl10 single mutants.
The increased frequency of aberrant type III axon innervation patterns in body-wall muscle 13 of third instar larvae single heterozygous for rl1 is further enhanced by heterozygosity for either Cdk23 or Gp93EY06213 but not Gαq1, each of these single heterozygotes on their own also display type III axon targeting defects.
One copy of rl1 further suppresses the enhanced wing pattern and ectopic wing vein phenotypes seen when flies expressing RetMEN2B.Scer\UAS is expressed under the control of Scer\GAL4C-765 are treated with AD58.
The structural defects seen in the neuromuscular junctions (NMJ) of Atg13 homozygous larvae are almost completely suppressed by rl1/rl1 : synaptic density and apposition of pre and post synaptic components are restored and NMJ size is partially restored. Despite the fact that evoked excitatory junction potentials at larval NMJs are decreased in both rl1 homozygous and Atg13 homozygous larvae, this phenotype is not see in rl1 Atg13 double homozygotes.
rl1 recessively enhances the eye and wing defects associated with the asp1/aspDD3 mutant. The brain neuroblast mitotic index of double mutants with asp1 is generally as for asp1 mutants alone. The rl1; asp1 double mutant also displays an increase in the number of mitotic figures that show lagging chromatids at anaphase, and a low frequency of abnormal mitotic figures where the telomeric ends of chromosomes appear to remain in contact, forming daisy chains. The frequency of aneuploid and polyploid figures is significantly increased compared to asp1 single mutants.
Egfrt1 enhances the bristle, ocellar, wing and limb phenotypes of homozygotes. Bristle hyperplasia, ocellus enlargement and ocellus fusion are enhanced. Wings are notched, wing margins are hairier and have ectopic vein material. Legs are fused and bifurcated.
rl1 partially suppresses the external eye phenotype and photoreceptor degeneration seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF. The progressive impairment of motor performance and reduction in lifespan seen when Hsap\ATXN182Q.Scer\UAS is expressed under the control of Scer\GAL4nrv2.PS are also partially rescued.
Scer\GAL4elav-C155-driven expression of rlScer\UAS.cKa efficiently rescues the ectopic targetting of type III axons to the abdominal body-wall muscle 13 but fails to rescue the premature defasciculation of axons bundles at the boundary between muscles 13 and 12 in rl1 mutant third instar larvae. Scer\GAL4Mef2.PU-controlled expression significantly improves both of these innervation defects.
Bridges, 23rd June 1922.