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General Information
Symbol
Dmel\rlSem
Species
D. melanogaster
Name
Sevenmaker
FlyBase ID
FBal0014598
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Sem, rolledSem
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G1113720A

Amino acid change:

D334N | rl-PC; D334N | rl-PD; D334N | rl-PE; D289N | rl-PG; D224N | rl-PH; D334N | rl-PI

Reported amino acid change:

D334N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: D334N. Amino acid 334 is located at the C-terminal end of kinase domain XI and is conserved in the different members of the MAP kinase family.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

rlSem mutants exhibit significant reductions in NMJ size and numbers of pre-synaptic active zones and post-synaptic GluRIID fields. The areas of the active zones and GluRIID fields are unchanged compared with controls.

rlSem/+ wings contain excess vein material.

Heterozygotes have thickened and ectopic wing venation, particularly in the anterior cross vein.

Heterozygous rlSem produces extra wing vein material.

Absolute bouton number and bouton number per muscle area is significantly increased in heterozygous larvae compared to controls at the neuromuscular junction in segment A3 in muscle 6/7.

Heterozygotes have ectopic veins in the wing.

rlSem/+ flies have ectopic vein material in the wing.

rlSem mutants exhibit extra wing veins.

The wings of rlSem/+ animals form scattered fragments of ecoptic vein tissue in intervein regions.

Heterozygotes have a rough eye phenotype, which is enhanced if these flies also express rlGMR.T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD or rlGMR.T:SV40\nls2,T:Scer\GAL4,T:Hsim\VP16,T:Hsim\gD.

rlSem flies have an extra wing vein phenotype. Additional R7 cells are formed in the eye.

At stage 17 rlSem mutant embryos have an average of 6 midline glial cells per segment (n=231), compared to 2.8 per segment in wild-type.

rlSem/+ mutants exhibit a significant increase in the number of synaptic boutons compared to wild-type.

10.7% of embryos derived from heterozygous females have less than 4 denticle belts.

Extra wing veins develop.

Heterozygous rlSem animals display a mild rough eye phenotype.

Flies carrying rlSem have a rough eye phenotype.

Heterozygous females show a weak dominant sterility; 10.2% of the eggs laid by these females fail to hatch and 20.0% of these nonviable embryos have defects similar to those associated with gain-of-function alleles of tor.

Mutants display no apparent proliferation defect. Adult have moderately rough eyes and extra wing vein material.

Shows supernumerary pigment cells in the developing eye, due to failure of apoptosis.

rlSem/+ shows ectopic wing vein tissue.

Adults exhibit a rough eye phenotype that cannot be suppressed by heterozygosity for Jra (Jra76-19).

Ommatidia differentiate additional R7 cells, phenotype suppressed by P{sE-D-junS82A,T92A,T107A}.

Rough eye phenotype is slightly suppressed by JrabZIP.hs.sev.T:Ivir\HA1.

The sev signalling pathway is activated, even in the absence of the boss inducing signal: flies show positive phototaxis towards UV light. Eyes show multiple R7 cells. Embryos from heterozygous females show reduced numbers of abdominal segments though the head skeleton and filzkorper do develop. As for dominant Egfr mutants, heterozygotes have extra wing vein material.

Ommatidia contain many R7 cells. rlSem/rl+;pnt1277/pntΔ88 double mutant flies possess either one or no R7 cells and occasionally lack R1-R6 cells.

Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, cell lethality and tergite bristle abnormalities.

Rough eyes and extra wing veins. Formation of supernumerary R7 photoreceptor cells.

rlSem is a dominant allele that causes the formation of R7 photoreceptors even in the absence of boss or sev. rlSem also causes extra wing vein material and a dominant tor phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

rlSem has visible | dominant phenotype, enhanceable by pum7/pum[+]

rlSem has visible phenotype, enhanceable by ed[+]/ed1X5

NOT Enhanced by
Statement
Reference

rlSem has visible phenotype, non-enhanceable by Sps1k11320

Suppressed by
Statement
Reference

rlSem has visible phenotype, suppressible by msk[+]/msk5

rlSem has visible phenotype, suppressible by msk[+]/msk2

NOT suppressed by
Statement
Reference

rlSem has visible phenotype, non-suppressible by msk5

rlSem has visible phenotype, non-suppressible by msk4

rlSem has visible phenotype, non-suppressible by Sps1k11320

Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

rl[+]/rlSem is a suppressor of visible | somatic clone phenotype of Ras85DD38E, Ras85Dx7b

rlSem is a suppressor of visible phenotype of lace2/lacek05305

rlSem is a suppressor of visible phenotype of PTP-ERhs.sev

rlSem is a suppressor of lethal phenotype of hidhs.PG

rlSem/rl+ is a suppressor of partially lethal phenotype of Raf107

rlSem/rl+ is a suppressor of partially lethal phenotype of Raf12

rlSem/rl+ is a suppressor of partially lethal phenotype of Raf1

NOT Suppressor of
Statement
Reference

rlSem/rl+ is a non-suppressor of lethal phenotype of slprBS06

rl[+]/rlSem is a non-suppressor of visible | recessive | somatic clone phenotype of Ras85Dx7b

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

rlSem has wing vein phenotype, enhanceable by Ptp61F[+]/Ptp61F4-10

rlSem has wing vein | ectopic phenotype, enhanceable by Ptp61F[+]/Ptp61F4-10

rlSem has wing vein | ectopic phenotype, enhanceable by pum7

rlSem has wing vein | ectopic phenotype, enhanceable by Gug35/Gug[+]

rlSem has wing vein | ectopic phenotype, enhanceable by Gug03928/Gug[+]

rlSem has wing vein | ectopic phenotype, enhanceable by Gug1207D6/Gug[+]

rlSem has wing vein | ectopic phenotype, enhanceable by Gug14967/Gug[+]

rlSem has wing vein | ectopic phenotype, enhanceable by ash2[+]/ash2I1

rlSem has wing vein | supernumerary phenotype, enhanceable by ed[+]/ed1X5

rlSem has wing vein phenotype, enhanceable by ed1X5

rlSem has phenotype, enhanceable by bs03267

NOT Enhanced by
Statement
Reference

rlSem has eye phenotype, non-enhanceable by Sps1k11320

rlSem has photoreceptor cell phenotype, non-enhanceable by Sps1k11320

rlSem has phenotype, non-enhanceable by lilliXS407/lilliXS407

rlSem has phenotype, non-enhanceable by lilliXS575

rlSem has phenotype, non-enhanceable by lillis35

rlSem has phenotype, non-enhanceable by Shc111-40

rlSem has phenotype, non-enhanceable by ShcBG

Suppressed by
Statement
Reference

rlSem has wing vein | ectopic phenotype, suppressible by GugUbi-p63E.GFP

rlSem has wing vein | ectopic phenotype, suppressible by kay2

rlSem has wing vein | ectopic phenotype, suppressible by msk[+]/msk5

rlSem has wing vein | ectopic phenotype, suppressible by msk[+]/msk2

rlSem has eye photoreceptor cell | ectopic phenotype, suppressible by Jra1

rlSem has eye phenotype, suppressible by TfIIA-SE73

NOT suppressed by
Statement
Reference

rlSem has wing vein | ectopic phenotype, non-suppressible by msk5

rlSem has wing vein | ectopic phenotype, non-suppressible by msk4

rlSem has wing vein | ectopic phenotype, non-suppressible by kayhs.PZ/kay2

rlSem has photoreceptor cell phenotype, non-suppressible by Sps1k11320

rlSem has wing vein | ectopic phenotype, non-suppressible by Sps1k11320

rlSem has eye phenotype, non-suppressible by Sps1k11320

rlSem has phenotype, non-suppressible by lilliXS407/lilliXS407

rlSem has phenotype, non-suppressible by lilliXS575

rlSem has phenotype, non-suppressible by lillis35

rlSem has phenotype, non-suppressible by Shc111-40

rlSem has phenotype, non-suppressible by ShcBG

rlSem has phenotype, non-suppressible by rin1

rlSem has phenotype, non-suppressible by rin2

Enhancer of
Statement
Reference

rl[+]/rlSem is an enhancer of wing phenotype of MadUAS.cNa, Scer\GAL4vg.int2.1

rl[+]/rlSem is an enhancer of wing vein | ectopic phenotype of MadUAS.cNa, Scer\GAL4vg.int2.1

rlSem is an enhancer of phenotype of svp1.sev

rlSem is an enhancer of phenotype of svp2.sev

rlSem is an enhancer of wing vein | ectopic phenotype of EgfrE3

rl[+]/rlSem is an enhancer of eye phenotype of EgfrE3

rlSem is an enhancer of wing vein | ectopic phenotype of EgfrE1

rl[+]/rlSem is an enhancer of eye phenotype of EgfrE1

NOT Enhancer of
Statement
Reference

rlSem is a non-enhancer of wing disc phenotype of ftk07918

rlSem is a non-enhancer of phenotype of Doa7/DoaHD

rlSem is a non-enhancer of phenotype of DoaHD/DoaDem

Suppressor of
Statement
Reference

rlSem is a suppressor of midline glial cell phenotype of commΔe39

rl[+]/rlSem is a suppressor of adult head | somatic clone phenotype of Ras85DD38E, Ras85Dx7b

rl[+]/rlSem is a suppressor of eye | somatic clone phenotype of Ras85DD38E, Ras85Dx7b

rlSem is a suppressor of phenotype of cswlf

rlSem is a suppressor of wing phenotype of lace2/lacek05305

rlSem is a suppressor of eye phenotype of PTP-ERhs.sev

rlSem is a suppressor of ommatidium phenotype of PTP-ERhs.sev

rlSem is a suppressor of ommatidium phenotype of hidGMR.PG/WGMR.PG

rlSem is a suppressor of eye phenotype of hidAla5.GMR

rlSem is a suppressor of eye phenotype of hidAla3.GMR

rlSem is a suppressor of eye phenotype of rprGMR.PW

rlSem is a suppressor of phenotype of vnddd-13

rlSem is a suppressor of phenotype of vnL6

NOT Suppressor of
Statement
Reference

rl[+]/rlSem is a non-suppressor of bouton | supernumerary phenotype of Fas2e76

rl[+]/rlSem is a non-suppressor of eye | somatic clone phenotype of Ras85Dx7b

rlSem is a non-suppressor of wing disc phenotype of ftk07918

rlSem is a non-suppressor of phenotype of Doa7/DoaHD

rlSem is a non-suppressor of phenotype of DoaHD/DoaDem

Other
Additional Comments
Genetic Interactions
Statement
Reference

Expressing Dsor1GD9123 under the control of Scer\GAL4en.PU in a rlSem/+ background results in notched wings with a mix of ectopic and lost vein tissue. Wings did not show creases, or the "droopy" phenotype.

rlSem in combination with Ptp61F4-10/+ or Scer\GAL4GMR.PU-driven Ptp61FdsRNA.Scer\UAS.WIZ or Ptp61FGD3571 results in larger than normal eyes.

Heterozygous pum7 greatly enhances the dominant extra wing vein phenotype of rlSem.

rlSem/+ does not suppress the lethality of slprBS06/Y males.

S6kIIign-Δ58-1/Y ; rlSem/+ double mutant larvae do not have a higher number of boutons at the neuromuscular junction than either single mutant.

rlSem/+ enhances the mutant wing phenotype caused by expression of MadScer\UAS.cNa under the control of Scer\GAL4vg.int2.1.

The ectopic wing vein material phenotype of flies expressing rlSem is enhanced in Gug03928, Gug35, Gug1207D6 and Gug14967 heterozygotes.

Expression of GugUbi-p63E.T:Avic\GFP suppresses the extra wing vein material formation seen in rlSem mutants.

Formation of ectopic vein tissue in rlSem/+ animals is enhanced by ash2I1/+.

The rlSem extra wing vein phenotype is unaffected by the expression of Mkp3Scer\UAS.cRa under the control of Scer\GAL4en-GAL4-33. The formation of additional R7 cells seen in rlSem flies is almost completely suppressed by the expression of Mkp3hs.sev.

The increase in midline glial cells seen in rlSem mutant embryos is slightly enhanced by Wunspecified/Wunspecified (from 6 (n=231) to 6.6 (n=91)). The reduction in midline glial cell numbers seen in commΔe39 mutant embryos is suppressed in double mutants with rlSem. These rescued midline glial cells are scattered throughout the midline, which lacks axons in these animals.

Double mutants of Fas2e76 rlSem do not exhibit a bouton number significantly different from that found in the Fas2e76 mutant alone.

rlSem/+ alone is not sufficient to rescue the reduced eye and head phenotypes of flies that are homozygous for Ras85Dx7b in the eye disc. However, the addition of Ras85DD38E in combination with rlSem results in a substantial rescue of the Ras85Dx7b eye and head size phenotypes; differentiated photoreceptor cells are seen and a substantial fraction of the ommatidia contain the full complement of photoreceptors.

The addition of one copy of SelDk11320 to rlSem/+ fliers has no effect on the eye phenotype.

Suppresses the wing margin incision and loss of microchaetae phenotypes of lacek05305/lace2 flies.

rlSem completely suppresses the PTP-ERhs.sev phenotype, to the point of producing ectopic R7 cells as seen in mutants in rlSem alone.

Suppression of WAla3.GMR and WAla5.GMR is very mild and noticeably less evident than that of WGMR.PG.

At 25[o]C rlSem dominantly enhances most of the hyperplastic growth seen in Ras85DV12.Scer\UAS Scer\GAL4dpp.blk1 wing discs resulting in a much larger disc with severe disruptions of morphology. Ras85DV12.G37.Scer\UAS Scer\GAL4dpp.blk1 and rlSem act synergistically to produce a small amount of hyperplastic growth in third instar wing discs. Mutants die as pharate adults at 25[o]C and have deletions of tarsal segments and extra wing vein material along L3.

Suppresses the eye reduction phenotype caused by rprGMR.PW. Suppresses the eye reduction phenotype caused by WGMR.PG.

Appearance of ectopic photoreceptor cells in the adult eye is suppressed by loss of a functional copy of Jra (Jra1 or JraIA109).

Suppresses the viability of vnddd-13 and proliferation defects in vnL6.

Enhancer of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

Eyes show multiple R7 cells, even in a sev- mutant background. rlSem suppresses the reduced viability associated with phl12, phl1 and phl107, and the eye phenotype of phl1. Homozygous tll1 embryos from rlSem/+ mothers have a tll mutant phenotype. The extra wing vein phenotype of heterozygotes is enhanced in transheterozygotes with dominant Egfr alleles. Flies heterozygous for EgfrE3 or EgfrE1 and rlSem have smaller eyes than EgfrE3 or EgfrE1 heterozygotes alone.

Xenogenetic Interactions
Statement
Reference

The ectopic wing vein phenotype seen when Hsap\ATN1N917.65Q.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF is enhanced in rlSem heterozygotes.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

The mutation in rlSu23 is identical to that found in rlSem.

Phenotypes of rl mutants fall into an allelic series: Df(2R)rl10a/rl1 >= rl9/rl1 > rlla8/rl1 > rl10/rl1 > rl1/rl1.

Hyperactivated form of the rl kinase.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
References (65)