Allele Dmel\ro¹

General Information
Symbol	Dmel\ro¹	Species	D. melanogaster
Name		FlyBase ID	FBal0014629
Feature type	allele	Created / Updated	2006-05-15/2006-05-15
Associated gene	Dmel\ro

Allele class
Mutagen	spontaneous
Nature of the Allele
Allele class
Mutagen	spontaneous
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains a >10 kb insertion homologous to 412 in intron 1 5' to homeobox (Tomlinson, Kimmel and Rubin, 1988).
Assay mode
Caused by insertion	412{}ro¹ (Lindsley and Zimm, 1992)
Carried on aberration	F(3R)2
Cytology
Phenotypic Data
Phenotypic Class
	tissue polarity defective \| recessive (Fanto et al., 1998)
Phenotype Manifest In
	photoreceptor cell R3 \| ectopic \| precursor (Fanto et al., 1998) photoreceptor cell R4 \| ectopic \| precursor (Fanto et al., 1998) photoreceptor cell R2 \| precursor (Fanto et al., 1998) photoreceptor cell R5 \| precursor (Fanto et al., 1998) ommatidium (Heberlein and Rubin, 1990) eye photoreceptor cell (Heberlein and Rubin, 1990)
Detailed Description
	Statement Reference Homozygotes have rough eyes; slightly smaller and narrower than wild type. Approximately normal numbers of facets but arrangement irregular; frequently have fewer and sometimes more than eight retinula cells; some facets missing such that three ommatidial bristles juxtaposed; other facets fused (Stemm-Tegethoff and Dicke, 1974; Ready, Hanson and Benzer, 1976). Fiber pathways through lamina and into medulla in considerable disarray; optic chiasma between the two replaced by parallel fibers; laminar cartridge and medullar columns deranged, ventral epithelial nuclear row absent; medulla displaced from normal position and rotated anteriorly. Mosaic studies demonstrate that phenotype is eye autonomous; i.e., the genotype of the eye dictates that of the underlying nervous elements. Disrupts an early stage of eye development: ommatidal array is disrupted, number of photoreceptors per ommatidia is variable. (Tomlinson et al., 1988) The ommatidial array is disrupted and the number of photoreceptor cells per ommatidium is variable. (Heberlein and Rubin, 1990) e⁴ wo¹ ro¹ mutant larvae show an abnormal response to UVC radiation. (Baden et al., 1996) The R2/R5 precursor pair is transformed into R3/R4 in most preclusters in homozygous third larval instar eye discs. The overall arrangement and rotation of the clusters is abnormal, with some clusters rotating in the wrong direction and others not rotating. Mosaic ommatidia with mutant R3 and R4 cells behave like wild-type with respect to ommatidial polarity. (Fanto et al., 1998) Does not appear to have a gross defect on brain development. (Page, 2003)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference ro¹ is an enhancer of visible phenotype of Scer\GAL4^sca-109-68, ato^Scer\UAS.cJa (White and Jarman, 2000)
Phenotype Manifest In
NOT Enhanced by
Statement Reference ro¹ has phenotype, non-enhanceable by ast^K6 (Kotarski et al., 1998)
Suppressed by
Statement Reference ro¹ has phenotype, suppressible \| partially by Dfd::ro^tLa (Lockett et al., 1993)
Enhancer of
Statement Reference ro¹ is an enhancer of eye phenotype of Scer\GAL4^sca-109-68, ato^Scer\UAS.cJa (White and Jarman, 2000) ro¹ is an enhancer of ommatidium phenotype of Scer\GAL4^sca-109-68, ato^Scer\UAS.cJa (White and Jarman, 2000)
Additional Comments
Genetic Interactions
Statement Reference The phenotype of ro¹ homozygotes is not dominantly altered by ast^K6. (Kotarski et al., 1998) Enhances the rough eye phenotype caused by expression of ato^Scer\UAS.cJa under the control of Scer\GAL4^sca-109-68. (White and Jarman, 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	ro¹ is rescued by ro^+t6.8 (Lockett et al., 1992) ro¹ is rescued by ro^+t8.6 (Tomlinson et al., 1988) ro¹ is rescued by ro^+tHa (Heberlein and Rubin, 1990)
Comments
Stocks ( 82 )
Bloomington	569 ro¹ 4064 ant¹/CyO; ro¹ 496 e⁴ wo¹ ro¹ 1713 ss¹ e⁴ ro¹/TM3, Sb¹ 595 se¹ ss¹ k¹ e^s ro¹ 609 st¹ Sb^sbd-1 e^s ro¹ ca¹ 3012 Df(3R)Dl-BX12, ss¹ e⁴ ro¹/TM6B, Tb¹
Kyoto	105984 ro¹ 107569 ant¹/CyO; ro¹ 105998 se¹ ss¹ k¹ e^s ro¹ 106007 st¹ Sb^sbd-1 e^s ro¹ ca¹ 101656 In(1)w^m4h ; se¹ ss¹ e¹ ro¹
	More stocks available...
Notes on Origin
Discoverer	Muller, June 1913.

Comments
	No interaction with P{sev-svp1} or P{sev-svp2} exists. (Begemann et al., 1995)
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ro¹
Name Synonym
Secondary FlyBase IDs

References ( 17 )
Research paper	Page, 2003, Curr. Biol. 13(6): 474--482 A function for EGF receptor signaling in expanding the developing brain in Drosophila. [FBrf0158813] Cook and Carpenter, 2002, D. I. S. 85: 34--37 Characterization of Tp(3;3)ME61 and genetic observations on chromosomal regions 96 and 97 of Drosophila melanogaster. [FBrf0179696] White and Jarman, 2000, Development 127(8): 1681--1689 Drosophila atonal controls photoreceptor R8-specific properties and modulates both receptor tyrosine kinase and hedgehog signalling. [FBrf0127389] Fanto et al., 1998, Mech. Dev. 74(1,2): 51--58 Linking cell-fate specification to planar polarity: determination of the R3/R4 photoreceptors is a prerequisite for the interpretation of the Frizzled mediated polarity signal. [FBrf0103504] Kotarski et al., 1998, Genome 41(2): 295--302 The Drosophila gene asteroid encodes a novel protein and displays dosage-sensitive interactions with Star and Egfr. [FBrf0102955] Baden et al., 1996, Arch. Insect Biochem. Physiol. 32(2): 187--196 Drosophila melanogaster larvae detect low doses of UVC radiation as manifested by a writhing response. [FBrf0086885] Begemann et al., 1995, Development 121(1): 225--235 The Drosophila orphan nuclear receptor Seven-up requires the Ras pathway for its function in photoreceptor determination. [FBrf0076133] Knibb et al., 1993, Molec. gen. Genet. 239(1-2): 109--114 Genetic analysis of chromosomal region 97D2-9 of Drosophila melanogaster. [FBrf0059080] Lockett et al., 1993, Mech. Dev. 41(1): 57--68 Rough genes with Deformed homeobox substitutions exhibit rough regulatory specificity during Drosophila eye development. [FBrf0058990] Lockett et al., 1992, Gene 114: 187--193 The rough (ro+<up>+</up>) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster. [FBrf0056088] Heberlein et al., 1991, Development 112(3): 703--712 Cell-fate determination in the developing Drosophila eye: role of the rough gene. [FBrf0053810] Heberlein and Rubin, 1990, Proc. Natl. Acad. Sci. USA 87: 5916--5920 Structural and functional comparisons of the Drosophila virilis and Drosophila melanogaster rough genes. [FBrf0052854] Saint et al., 1988, Nature 334: 151--154 Pattern formation in the developing eye of Drosophila melanogaster is regulated by the homoeo-box gene, rough. [FBrf0048747] Tomlinson et al., 1988, Cell 55: 771--784 rough, a Drosophila homeobox gene required in photoreceptors R2 and R5 for inductive interactions in the developing eye. [FBrf0047664] Kankel and Lipshitz, 1981, Hotta, 1981: 216--238 Allelic variability at loci affecting the development and function of the visual system. [FBrf0084083] Bridges and Morgan, 1923, Publs Carnegie Instn 327: 1--251 The third-chromosome group of mutant characters of Drosophila melanogaster. [FBrf0001261]
Book	Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster. The Genome of Drosophila melanogaster. [FBrf0066905]

Allele Dmel\ro1

Allele Dmel\ro¹