FlyBase Allele Report: Dmel\Polr2B[S1]

General Information

Symbol

Dmel\Polr2B^S1

Species

D. melanogaster

Name

FlyBase ID

FBal0014649

Feature type

allele

Associated gene

Dmel\Polr2B

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Krasnoselskaya et al., 1998, Mortin, 1990)

Progenitor genotype

Cytology

Description

Mutant lesions in RpII140^S1, RpII140^S2, RpII140^S5, RpII140^S6 and RpII140^S8 are identical. Nucleotide substitution: A2966T.

(Krasnoselskaya et al., 1998)

Amino acid replacement: S728C. Nucleotide substitution: A2966T.

(Kim et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,128,255..14,128,255 [+]

Nucleotide change:

A14128255T

Reported nucleotide change:

A2966T|FBrf0103162

Amino acid change:

S728C | Polr2B-PA; S728C | Polr2B-PB

Reported amino acid change:

S728C|FBrf0103162

(Kim et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Mortin, 1990)

Phenotype Manifest In

Detailed Description

Statement

Reference

recovered as a dominant suppressor of RpII215^K1

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Polr2B^S1 is a suppressor of lethal phenotype of Polr2A^K1

(Kim et al., 1994)

NOT Suppressor of

Statement

Reference

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A¹

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A¹⁰⁶

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A¹⁰⁸

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A¹⁰⁹

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A¹²

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A⁶⁶

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A^Ubl

(Kim et al., 1994)

Polr2B^S1 is a non-suppressor of lethal phenotype of Polr2A^ts

(Kim et al., 1994)

Phenotype Manifest In

Enhancer of

Statement

Reference

Polr2B^S1 is an enhancer of phenotype of Polr2A²⁷

(Krasnoselskaya et al., 1998)

Suppressor of

Statement

Reference

Polr2B^S1 is a suppressor of phenotype of Polr2A¹²⁰

(Krasnoselskaya et al., 1998)

Polr2B^S1 is a suppressor of phenotype of Polr2A^K1

(Krasnoselskaya et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Strong suppressor: restores viability to greater than 49% of wild type flies.

(Krasnoselskaya et al., 1998)

The recessive lethality of RpII215¹, RpII215^Ubl, RpII215^ts, RpII215⁶⁶, RpII215¹⁰⁶, RpII215¹⁰⁸, RpII215¹² or RpII215¹⁰⁹ is not rescued by one copy of RpII140^S1. The recessive lethality of RpII215^K1 is rescued to more than 50% viability by one copy of RpII140^S1.

(Kim et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Isolated as a dominant suppressor of the conditional lethality of RpII215^K1.

(Mortin, 1990)

RNA polymerase II purified from RpII215⁴ embryos has a higher elongation rate than normal and is more efficient at reading through intrinsic elongation blocks. The mutant enzyme is less responsive than the wild-type enzyme to TfIIS and NH₄⁺ ions. RNA polymerase II purified from RpII140^S1 embryos is faster than wild-type RNA polymerase II or RNA polymerase II purified from RpII215⁴ embryos at generating the final pattern of shortened transcripts during extended TfIIS mediated pyrophosphorolysis. These results suggest that the RpII140^S1 mutation increases the time spent by RNA polymerase II in an elongation competent mode.

(Chen et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Polr2B^S1

RpII140^S1

(Mortin, 1990)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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