Allele Dmel\RpII215¹²

General Information
Symbol	Dmel\RpII215¹²	Species	D. melanogaster
Name		FlyBase ID	FBal0014669
Feature type	allele	Associated gene	Dmel\RpII215
Also Known As	RpII215^E28, E28

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Voelker et al., 1985, Chen et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:11,462,272..11,462,272 evidence=experimental na_change=G11462272A pr_change=A377T\|RpII215-PA reported_na_change=G2880A reported_pr_change=A377T (Chen et al., 1993)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	RpII215⁴ (Mortin and Kaufman, 1982, Voelker et al., 1985, Chen et al., 1993)
Nature of the lesion	Statement Reference Nucleotide substitution: G2880A. (Krasnoselskaya et al., 1998) Amino acid replacement: A377T. Nucleotide substitution: G2880A. (Chen et al., 1993)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant (Voelker et al., 1985) lethal \| heat sensitive (Mortin, 1990) lethal \| recessive (Kim et al., 1994) lethal \| recessive \| heat sensitive (Lindsley and Zimm, 1992, Voelker et al., 1985, Chen et al., 1993, Krasnoselskaya et al., 1998)
Phenotype Manifest In
	capitellum (Chen et al., 1993)
Detailed Description
	Statement Reference Selected for loss of α-amanitin resistance. (Krasnoselskaya et al., 1998) Exhibits the Ubx effect: heterozygotes partially transform the capitellum of the haltere into the wing blade. (Chen et al., 1993) Haplospecific lethal. Survives, at least with a low frequency, as a homozygote, but is lethal in hemizygotes. (Lindsley and Zimm, 1992) Mutant flies show resistance to α-amanitin. (Voelker et al., 1985) Temperature-sensitive mutation. (Mortin and Kaufman, 1982)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference RpII215¹² has lethal phenotype, suppressible \| partially by RpII140^S7 (Kim et al., 1994) RpII215¹² has lethal phenotype, suppressible \| partially by RpII140^S11 (Kim et al., 1994) RpII215¹² has lethal phenotype, suppressible \| partially by RpII140^S12 (Kim et al., 1994) RpII215¹² has lethal phenotype, suppressible by RpII140^M (Kim et al., 1994)
NOT suppressed by
Statement Reference RpII215¹² has lethal phenotype, non-suppressible by RpII140^S1 (Kim et al., 1994) RpII215¹² has lethal phenotype, non-suppressible by RpII140^S2 (Kim et al., 1994) RpII215¹² has lethal phenotype, non-suppressible by RpII140^S5 (Kim et al., 1994) RpII215¹² has lethal phenotype, non-suppressible by RpII140^S6 (Kim et al., 1994) RpII215¹² has lethal phenotype, non-suppressible by RpII140^S8 (Kim et al., 1994)
Phenotype Manifest In
NOT suppressed by
Statement Reference RpII215¹² has phenotype, non-suppressible by RpII140^SM (Krasnoselskaya et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference The recessive lethality of RpII215¹² is not rescued by one copy of RpII140^S1, RpII140^S2, RpII140^S5, RpII140^S6 or RpII140^S8. The recessive lethality of RpII215¹² is rescued to between 1 and 49% viability by one copy of RpII140^S7, RpII140^S11 or RpII140^S12. The recessive lethality of RpII215^K1 is suppressed by RpII140^M. (Kim et al., 1994)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	3661 v¹ RpII215¹²/FM7a/Dp(1;Y)y⁺v⁺#3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	E28 (Chen et al., 1993, Voelker et al., 1985) RpII215¹² (Popodi et al., 2010-) RpII215^E28 (Lindsley and Zimm, 1992, Mortin, 1990) RpII^E28 (Mortin and Kaufman, 1982) Ubl^E28
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Krasnoselskaya et al., 1998, Mol. Gen. Genet. 258(5): 457--465 Selection and analysis of rare second-site suppressors of Drosophila RNA polymerase II mutations. [FBrf0103303] Kim et al., 1994, J. Mol. Biol. 244(7): 13--22 Molecular modeling of RNA polymerase II mutations onto DNA polymerase I. [FBrf0103162] Chen et al., 1993, Mol. Cell. Biol. 13(7): 4214--4222 Mapping mutations in genes encoding the two large subunits of Drosophila RNA polymerase II defines domains essential for basic transcription functions and for proper expression of developmental genes. [FBrf0059056] Mortin, 1990, Proc. Natl. Acad. Sci. U.S.A. 87: 4864--4868 Use of second-site suppressor mutations in Drosophila to identify components of the transcriptional machinery. [FBrf0052847] Voelker et al., 1985, Mol. Gen. Genet. 201: 437--445 Genetic and molecular variation in the RpII215 region of Drosophila melanogaster. [FBrf0043258] Mortin and Kaufman, 1982, Mol. Gen. Genet. 187(1): 120--125 Developmental genetics of a temperature-sensitive RNA polymerase II mutation in Drosophila melanogaster. [FBrf0038443]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621]
Book	Lindsley and Zimm, 1992, The Genome of Drosophila melanogaster. The Genome of Drosophila melanogaster. [FBrf0066905]

Allele Dmel\RpII21512

Allele Dmel\RpII215¹²