Allele Dmel\rst⁶

General Information
Symbol	Dmel\rst⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0014797
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\rst

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Lefevre and Green, 1972, Wolff and Ready, 1991)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Point mutant. (Welshons and Welshons, 1986) Disrupts the intracellular domain. (Ramos et al., 1993) 98bp deletion that severely affects the cytoplasmic domain. (Ramos et al., 1993)
Assay mode
Cytology	Polytene chromosomes normal. Polytene chromosomes normal. (Lefevre and Green, 1972)
Phenotypic Data
Phenotypic Class
	visible \| recessive (Tanenbaum et al., 2000)
Phenotype Manifest In
	interommatidial bristle \| ectopic (Tanenbaum et al., 2000) ommatidium (Tanenbaum et al., 2000) antennal segment 3 sensillum (Reddy et al., 1999) sensillum basiconicum of antennal segment 3 (Reddy et al., 1999) antennal disc (Reddy et al., 1999) eye disc (Grzeschik and Knust, 2005) embryonic muscle system (Strunkelnberg et al., 2001) embryonic somatic muscle \| ectopic (Strunkelnberg et al., 2001) ommatidial cluster (Vishnu et al., 2006)
Detailed Description
	Statement Reference viability normal in both sexes no vt effect Timing of adult emergence is normal. (Lefevre and Green, 1972) e(fa^swb)¹ rst⁶ flies have slightly rough eyes. (Welshons and Welshons, 1986) No defects in optic chiasm. (Boschert et al., 1990) Occasional extra secondary pigment cells within the eye. Low levels of cell death are seen in the larval eye disc leading to a disorganised retinal lattice. (Wolff and Ready, 1991) Spacing of arcs along the posterior of the morphogenetic furrow appears normal. (Wolff and Ready, 1991) Individuals homozygous for Bpt¹ and rst⁶ exhibit no Bpt¹ phenotype. (Duus et al., 1992) Rough eye phenotype. (Ramos et al., 1993) Severe eye phenotype. (Reiter et al., 1996) The total number of sensilla on the antenna are comparable to wild-type controls, but spacing between adjacent sensilla is altered and there are regions where the sense organs appear in tufts. Clusters of 6 to 8 sensilla basiconica often appear to fall into a pit in the epidermis. The total number of sensory founder cells in the antennal disc is comparable to wild type, but the pattern of the founder cells is disorganised. The pattern of apoptotic nuclei in third larval instar antennal discs is no different from wild type. (Reddy et al., 1999) Many ectopic interommatidial bristles are visible throughout the interommatidial lattice in mature mutant pupal retinas. Adult eyes have a rough surface with misaligned ommatidial rows. (Tanenbaum et al., 2000) Homozygous muscles show thin muscles and sometimes lack muscles entirely in some segments. Furthermore, embryos exhibit muscles in ectopic positions. (Strunkelnberg et al., 2001) Mutant pupal eye discs do not show elimination of surplus interommatidial (lattice) cells (which occurs in wild-type discs) and disruption of cell sorting is seen. (Grzeschik and Knust, 2005) In the eye disc of hemizygous rst[6] flies, cell sorting does not occur, leading to an excess of surviving interommatidial precursor cells. In contrast, rst[6]/+ eye discs display no strong sorting defects and only occasionally have supernumerary interommatidial precursor cells. (Vishnu et al., 2006)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference rst⁶ has ommatidial cluster phenotype, enhanceable by Scer\GAL4^MZ1369/X11L^{dsRNA.Scer\UAS} (Vishnu et al., 2006)
Enhancer of
Statement Reference rst⁶/rst[+] is an enhancer of ommatidial cluster phenotype of Scer\GAL4^GMR.PF, X11L^{dsRNA.Scer\UAS} (Vishnu et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Expression of one copy of X11L[dsRNA.Scer\UAS], driven by Scer\GAL4[GMR.PF], in a rst[6]/+ background leads to severe cell sorting defects in eye discs, similar to those seen in rst[6] homozygotes. (Vishnu et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 4 )
Bloomington	1528 gt^Q292 rst⁶/FM7a 1530 y¹ gt^E6 rst⁶
Kyoto	106368 gt^Q292 rst⁶/FM7a 106370 y¹ gt^E6 rst⁶
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	irreC^CT (Grzeschik and Knust, 2005) irreC-rst^CT (Tanenbaum et al., 2000) rst⁶ (Christensen et al., 2008.6.11) rst^16172.646 (Lefevre and Green, 1972, ) rst^CT (Vishnu et al., 2005, Dworak et al., 2001, Frohlich, 2001, Reddy et al., 1999, Reiter et al., 1996, Araujo et al., 1996, Schneider et al., 1995, Ramos et al., 1993, Ramos et al., 1993, Ramos et al., 1991, Wolff and Ready, 1991, Wolff and Ready, 1991, Ramos et al., 1991, Boschert et al., 1990, Welshons and Welshons, 1986, Vishnu et al., 2006) rst^ct (Duus et al., 1992)
Name Synonym
Secondary FlyBase IDs

References ( 23 )

Generate a list of	All references relating to this allele ( 23 )

List References by type	Research paper ( 17 ) Personal communication to FlyBase ( 1 ) Abstract ( 5 )
Recent research papers ( 1 )
	Vishnu et al., 2006, Dev. Biol. 289(2): 296--307 The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila. [FBrf0190176] Show all research papers ...

Allele Dmel\rst6

Allele Dmel\rst⁶