The increase in the number of midline glia per ventral nerve cord segment seen in embryos expressing jingScer\UAS.cSa under the control of Scer\GAL4sli.PS is not suppressed by SIIN, but expression of jingScer\UAS.cSa under the control of Scer\GAL4sli.PS in a homozygous SIIN background does not induce extra midline glia and the number of midline glia per ventral nerve cord segment in these double mutant embryos is reduced compared to wild type and is similar to that seen in homozygous SIIN single mutants. SIIN/Df(2R)ST1 double heterozygous embryos have a reduced number of midline glia per segment compared to wild type. SIIN/jing3 and SIIN/jing01094 double heterozygous embryos have breaks in the dorsal trunk of the tracheal system at stage 15.
salm3 SIIN double mutant embryos have an average of 3.05 +/- 0.20 neurons per lch5 organ, similar to SIIN single mutant embryos. The embryos show misplacement of the lch5 organ along the dorsoventral axis comparable to the phenotype seen in salm single mutants.
Embryos expressing EgfrDN.Scer\UAS.cBa under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B show a partial reduction in the development of muscles DA1 and VA2. This phenotype is dominantly enhanced by SIIN. Dominantly enhances the mild muscle phenotype of Egfrf1 embryos raised at 18oC. Further enhancement of the phenotype is also seen if the flies are also heterozygous for spi1.