FlyBase Allele Report: Dmel\sa[1]

General Information

Symbol

Dmel\sa¹

Species

D. melanogaster

Name

FlyBase ID

FBal0015120

Feature type

allele

Associated gene

Dmel\sa

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Lin et al., 1996)

Progenitor genotype

Cytology

Description

Nucleotides 145-558 are deleted and replaced with a 158bp insertion. This leads to a frameshift after codon 47.

(Hiller et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | adult stage (with sa²)

(Lu and Fuller, 2015)

abnormal meiotic cell cycle | recessive

(Lin et al., 1996)

male sterile (with sa²)

(Hiller et al., 2004)

male sterile | recessive

(Lin et al., 1996)

Phenotype Manifest In

nuclear lamina | adult stage | male (with sa²)

(Trost et al., 2016)

nucleolus | adult stage (with sa²)

(Lu and Fuller, 2015)

spermatocyte

(Lin et al., 1996)

spermatocyte (with sa²)

(Hiller et al., 2004)

spermatocyte | adult stage (with sa²)

(Lu and Fuller, 2015)

testis

(Lin et al., 1996)

testis (with sa²)

(Hiller et al., 2004)

Detailed Description

Statement

Reference

sa¹/sa² adult heterozygote males show morphological defects of the nuclear lamina in stage S4-S6 spermatocytes (the lamina has distinctly more ballon-like shape, lacking the deformations that correlate with chromosome distribution seen in wild-type), while in stage S3 the lamina is of wild-type appearance.

(Trost et al., 2016)

sa¹/sa² mutant spermatocytes exhibit meiotic arrest and other defects, including bivalent chromosomes condensing to spherical shapes away from the nuclear periphery, and failure of the nucleolus to fully break down.

(Lu and Fuller, 2015)

sa¹/sa² males are sterile due to arrested spermatogenesis at spermatocyte stage.

(Hiller et al., 2004)

Early stages of spermatogenesis up through mature primary spermatocytes are present and normal. Postmeiotic stages are completely absent. Mutant testes have an abundance of mature primary spermatocytes with large, round nuclei and prominent nucleoli. Triple mutant aly¹, can¹, sa¹ homozygotes are female fertile and male sterile, with similar effects on testis squashes as sa¹ and can¹. Spermatocytes arrest in the G₂/M transition of meiosis I with partial chromosome condensation.

(Lin et al., 1996)

male-sterile Primary spermatocytes arrested premeiotically but apparently after attaining full size; testes become filled with mature primary spermatocytes.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sa²/sa¹ is rescued by sa^EGFP

(Chen et al., 2005)

sa²/sa¹ is rescued by sa^Tag:HA

(Chen et al., 2005)

sa²/sa¹ is rescued by sa^+t3.2

(Hiller et al., 2004)

Comments

sa^+t3.2 fully rescues the spermatocyte arrest and male sterility phenotypes of sa¹/sa² males.

(Hiller et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (3)

1762

106494

106419

y¹; l(2)*^*/CyO; sa¹/TM6; sv^spa-pol

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

VO45

sa¹

(Berry et al., 2022, Fingerhut et al., 2019, Trost et al., 2016, Lu and Fuller, 2015, Katzenberger et al., 2012, Moon et al., 2011, Di Cara et al., 2010, Rautureau et al., 2009, Boutanaev et al., 2007, Mikhaylova et al., 2006, Chen et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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