FlyBase Allele Report: Dmel\sas[15]

General Information

Symbol

Dmel\sas¹⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0015134

Feature type

allele

Associated gene

Dmel\sas

Associated Insertion(s)

Carried in Construct

Also Known As

EdR16

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lee et al., 2013)

Mutagen

ethyl methanesulfonate

(Lewis et al., 1980)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?643term.

(Lee et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:7,178,718..7,178,718 [+]

Nucleotide change:

C7178718T

Amino acid change:

R643term | sas-PA; R643term | sas-PB; R643term | sas-PC; R643term | sas-PD

Reported amino acid change:

?643term

Comment:

Mutation reported as having a stop codon at residue 643. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage 16

(Lee et al., 2013)

abnormal neuroanatomy | embryonic stage 16 (with Df(3R)ED5221)

(Lee et al., 2013)

lethal | recessive

(Cavener et al., 1986, Lewis et al., 1980)

lethal - all die before end of larval stage

(Schonbaum et al., 1992)

some die during larval stage

(Schonbaum et al., 1992)

Phenotype Manifest In

larval anterior commissure | embryonic stage 16

(Lee et al., 2013)

larval anterior commissure | embryonic stage 16 (with Df(3R)ED5221)

(Lee et al., 2013)

larval longitudinal connective | embryonic stage 16

(Lee et al., 2013)

larval longitudinal connective | embryonic stage 16 (with Df(3R)ED5221)

(Lee et al., 2013)

Detailed Description

Statement

Reference

sas¹⁵/Df(3R)ED5221 transheterozygotes appear normal at embryonic stage 14, but display weak longitudinal axon defects at stage 16. The outer Fas2-positive axon bundle is interrupted and the other bundles appear slightly irregular. Complete breaks of a longitudinal tract are rare, but do occasionally occur. Fas2 positive axons ectopically cross the midline in ~15% of segments. Defects in the anterior commissure are also seen.

sas¹⁵ homozygotes appear normal at embryonic stage 14, but display weak longitudinal axon defects at stage 16. The outer Fas2 positive axon bundle is interrupted and the other bundles appear slightly irregular. Complete breaks of a longitudinal tract are rare, but do occasionally occur. Fas2-positive axons ectopically cross the midline in ~15% of segments. Defects in the anterior commissure are also seen.

(Lee et al., 2013)

Larvae die during the first instar moult or as small second instar larvae.

(Schonbaum et al., 1992)

Lethality occurs during embryonic and larval stages.

(Cavener et al., 1986)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sas¹⁵ has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Lee et al., 2013)

sas¹⁵/Df(3R)ED5221 has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Ptp10D¹

(Lee et al., 2013)

sas¹⁵ has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Ptp10D¹

(Lee et al., 2013)

sas¹⁵/Df(3R)ED5221 has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Lee et al., 2013)

Suppressed by

Statement

Reference

Ptp69D¹/Df(3L)8ex25, sas¹⁵/Df(3R)ED5221 has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible | partially by sas^UAS.cLa/Scer\GAL4^Fas2-Mz507

(Lee et al., 2013)

Ptp69D¹/Df(3L)8ex25, sas¹⁵ has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible | partially by sas^UAS.cLa/Scer\GAL4^Fas2-Mz507

(Lee et al., 2013)

NOT Enhancer of

Statement

Reference

sas¹⁵/sas[+] is a non-enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

sas¹⁵/sas[+] is a non-enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

sas¹⁵ is a suppressor of abnormal cell adhesion phenotype of Cad99C^UAS.fl, Scer\GAL4^fkh.PH

(Chung and Andrew, 2014)

NOT Suppressor of

Statement

Reference

sas¹⁵ is a non-suppressor of abnormal cell adhesion phenotype of Cad99C^{EXTRA.UAS.Tag:HA}, Scer\GAL4^fkh.PH

(Chung and Andrew, 2014)

sas¹⁵/sas[+] is a non-suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

sas¹⁵/sas[+] is a non-suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

sas¹⁵ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Lee et al., 2013)

sas¹⁵ has larval anterior commissure | embryonic stage 16 phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Lee et al., 2013)

sas¹⁵/Df(3R)ED5221 has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Ptp10D¹

(Lee et al., 2013)

sas¹⁵ has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Ptp10D¹

(Lee et al., 2013)

sas¹⁵/Df(3R)ED5221 has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Lee et al., 2013)

Suppressed by

Statement

Reference

Ptp69D¹/Df(3L)8ex25, sas¹⁵/Df(3R)ED5221 has larval longitudinal connective | embryonic stage 16 phenotype, suppressible | partially by sas^UAS.cLa/Scer\GAL4^Fas2-Mz507

(Lee et al., 2013)

Ptp69D¹/Df(3L)8ex25, sas¹⁵ has larval longitudinal connective | embryonic stage 16 phenotype, suppressible | partially by sas^UAS.cLa/Scer\GAL4^Fas2-Mz507

(Lee et al., 2013)

NOT Enhancer of

Statement

Reference

sas¹⁵/sas[+] is a non-enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

sas¹⁵/sas[+] is a non-enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

sas¹⁵ is a suppressor of embryonic/larval salivary gland phenotype of Cad99C^UAS.fl, Scer\GAL4^fkh.PH

(Chung and Andrew, 2014)

NOT Suppressor of

Statement

Reference

sas¹⁵ is a non-suppressor of embryonic/larval salivary gland phenotype of Cad99C^{EXTRA.UAS.Tag:HA}, Scer\GAL4^fkh.PH

(Chung and Andrew, 2014)

sas¹⁵/sas[+] is a non-suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

sas¹⁵/sas[+] is a non-suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Salivary glands in sas¹⁵ mutant embryos expressing Cad99C^Scer\UAS.fl under the control of Scer\GAL4^fkh.PH are largely normal.

A sas¹⁵ mutant background does not affect the Cad99C^{EXTRA.Scer\UAS.T:Ivir\HA1} overexpression phenotype.

(Chung and Andrew, 2014)

Ptp10D¹ enhances the ectopic midline crossing phenotype that is seen in sas¹⁵/Df(3R)ED5221 mutant stage 16 embryos. Multiple Fas2-positive axon bundles cross the midline in each segment, and these are perpendicular to the longitudinal tracts. The inner Fas2-positive bundle is intact, but one or both of the outer longitudinal bundles are missing.

Ptp10D¹ enhances the ectopic midline crossing phenotype seen in homozygous sas¹⁵ mutant stage 16 embryos.

Ptp69D¹/Df(3L)8ex25 enhances the ectopic midline crossing phenotype seen in sas¹⁵/Df(3R)ED5221 mutant stage 16 embryos.

Ptp69D¹/Df(3L)8ex25 enhances the ectopic midline crossing phenotype seen in homozygous sas¹⁵ mutant stage 16 embryos. Segments of the outer longitudinal tract are missing. Multiple Fas2-positive axon bundles cross the midline in each segment, and these are perpendicular to the longitudinal tracts. The inner Fas2-positive bundle is intact, but one or both of the outer longitudinal bundles are missing. Complete breaks in the Fas2-positive longitudinal tract are seen. The anterior and posterior commissures are fused into a single commissural tract.

Expression of sas^Scer\UAS.cLa under the control of Scer\GAL4^Fas2-Mz507 almost completely rescues the CNS defects seen in sas¹⁵/Df(3R)ED5221, Ptp69D¹/Df(3L)8ex25 double mutant embryos.

Expression of sas^Scer\UAS.cLa under the control of Scer\GAL4^Fas2-Mz507 almost completely rescues the CNS defects seen in sas¹⁵, Ptp69D¹/Df(3L)8ex25 double mutant embryos.

(Lee et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2098

sas¹⁵ p^p cu¹/TM3, Sb¹ Ser¹

Kyoto

106663

sas¹⁵ p^p cu¹/TM3, Sb¹

Notes on Origin

Discoverer

R. Lewis.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EdR16

(Schonbaum et al., 1992, Lewis et al., 1980)

Sas¹⁵

(Swaminathan et al., 2010)

l(3)84Cd^r16

(Cavener et al., 1986)

l(3)r16

sas¹⁵

(Chung and Andrew, 2014, Lee et al., 2013, Christensen et al., 2008.6.11, Gresens and Cook, 2006.12.5)

Name Synonyms

Secondary FlyBase IDs

References (9)

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