FlyBase Allele Report: Dmel\sc[sisB-1]

General Information

Symbol

Dmel\sc^sisB-1

Species

D. melanogaster

Name

FlyBase ID

FBal0015251

Feature type

allele

Associated gene

Dmel\sc

Associated Insertion(s)

Carried in Construct

Also Known As

sis-b^sc3-1, sc^3-1

Genomic Maps

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Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Lindsley and Zimm, 1992, Garcia-Bellido, 1979)

Mutagen

X ray

(Lindsley and Zimm, 1992)

spontaneous

(Lindsley and Zimm, 1992)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

X:396,582..396,582 [+]

Nucleotide change:

A396582T

Amino acid change:

K136term | sc-PA

Reported amino acid change:

K136term

Comment:

sc^sisB-1 contains a point mutation and a 12kb tandem duplication containing the sc^sisB-1 allele and the adjacent wild type l(sc) transcription unit. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wrischnik et al., 2003)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

sc³

(Lindsley and Zimm, 1992)

Cytology

Nature of the lesion

Statement

Reference

The chromosome carries a tandem duplication (approximately 12kb) which includes a mutated form of sc (each copy carries the amino acid replacement K136term) and the adjacent wild-type l(1)sc transcription unit.

(Wrischnik et al., 2003)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior dorsocentral bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

anterior notopleural bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

anterior orbital bristle

(Wrischnik et al., 2003)

anterior orbital bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

anterior supraalar bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

anterior vertical bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

humeral bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

lower humeral bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

macrochaeta

(Erickson and Cline, 1991)

notopleural bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

ocellar bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

ocellar bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

orbital bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

postalar bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

posterior orbital bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

posterior postalar bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

postvertical bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

postvertical bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

presutural bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

presutural bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

supraalar bristle (with Df(1)sc19)

(Wrischnik et al., 2003)

upper humeral bristle

(Wrischnik et al., 2003)

upper humeral bristle (with In(1)sc^8Lsc^4R)

(Wrischnik et al., 2003)

Detailed Description

Statement

Reference

At 18^oC 76% of expected sc^sisB-2 homozygotes survive to adulthood, but this drops to 35% when these animals are raised at 25^oC. 50-89% of sc^sisB-1 homozygous adults lack upper humeral and anterior orbital bristles. With variable penetrance, sc^sisB-1/In(1)sc^8Lsc^4R adults lack ocellar, anterior orbital, posterior orbital, postvertical, upper humeral, anterior supraalar, notopleural, posterior postalar, lower humeral and presutral bristles. With variable penetrance sc^sisB-1/Df(1)sc19 adults lack all head bristles apart from the posterior vertical bristles, and all bristles of the dorsal thorax apart from the posterior notoplural, posterior dorsocentral and scutellar bristles.

(Wrischnik et al., 2003)

Temperature-sensitive mutation.

(Deshpande et al., 1995)

Embryonic lethality of females rescued by maternal ectopic expression of ac or l(1)sc. Maternal ac or sc can rescue sc^sisB-1 females.

(Parkhurst et al., 1993)

Original hypomorphic allele recovered as a reversion of sc³ to nearly wild-type ac and sc phenotype in hemizygote and homozygote. Locus also characterized by dominant effects of deficiencies and duplications of the ASC region and later by mutant alleles sc^10-1 and ac^Hw-49c that affect ASC functions more than sc^sisB-1 functions. sc^sisB-1 reduces viability of homozygous females and hemizygous females are lethal; yet hemizygous males fully viable. Dominant synergistic female-specific lethal interactions with loss-of-function alleles of Sxl, sisA, and/or maternal da; magnitude of viability effects depends on genetic background and inversely correlates with background effects on male-lethal effects; interactions temperature-dependent, generally more extreme at higher temperatures. Female viability effects suppressed by gain-of-function Sxl^M1 allele and by duplications of Sxl⁺ or sisA⁺. Duplication of sc^sisB-1⁺ male-lethal in combination with duplication of Sxl⁺ and/or sisA⁺, more so at lower temperatures. Male lethality of duplication combinations suppressed by Sxl^-. Phenotype of 2X;3A intersexes strongly dependent on dose of sc^sisB-1⁺. The dose-dependent interactions of this gene identify it as a positive regulator of Sxl⁺ and part of the numerator of what is referred to as the X/A balance, the primary sex-determination signal. This is a character it shares with sisA. RK2.

(Lindsley and Zimm, 1992)

Temperature shift experiments showed that lowering sc activity in the presence of extra copies of dpn⁺ results in the death of females but not males.

(Younger-Shepherd et al., 1992)

The viability of homozygous females ranges from 100% at 18^oC to less than 1% at 29^oC.

(Bopp et al., 1991)

Flies show a mild bristle phenotype. Sex determination is affected much more strongly by sc^sisB-1 than is neurogenesis, and the two aspects show different temperature dependencies. Temperature sensitive period for feamle lethal affect corresponds to peak of sc expression between nuclear cycles 9 and 14 in the embryo.

(Erickson and Cline, 1991)

Interacts with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

80% of female sc^sisB-1/sc^10-1 embryos die. All female sc^sisB-1/sc^10-1 embryos heterozygous for Sxl^f1 die. Female embryos heterozygous for sc^sisB-1 and Sxl^f1, and deficient for one copy of sisA show 75% lethality.

(Torres and Sanchez, 1991)

Homozygous females show reduced viability hemizygous female lethal.

(Garcia-Bellido, 1979)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable | maternal effect by mle¹/mle[+]

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable | maternal effect by msl-1^L60/msl-1[+]

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable | maternal effect by msl-2[+]/msl-2¹

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable | maternal effect by msl-3[+]/msl-3¹

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable by mof[+]/mof²

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable by roX1[+]/lncRNA:roX1^ex6

(Gladstein et al., 2010)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype, enhanceable by lncRNA:roX2^unspecified/roX2[+]

(Gladstein et al., 2010)

Suppressed by

Statement

Reference

(Gladstein et al., 2010)

sc^sisB-1 has lethal | female phenotype, suppressible by dpn[+]/dpn³

(Barbash and Cline, 1995)

sc^sisB-1 has lethal | female phenotype, suppressible by dpn[+]/dpn⁴

(Barbash and Cline, 1995)

sc^sisB-1 has lethal | female phenotype, suppressible by dpn⁵/dpn[+]

(Barbash and Cline, 1995)

sc^sisB-1 has lethal | female phenotype, suppressible by dpn[+]/dpn⁶

(Barbash and Cline, 1995)

sc^sisB-1 has lethal | female phenotype, suppressible by dpn⁷/dpn[+]

(Barbash and Cline, 1995)

Suppressor of

Statement

Reference

sc^sisB-1 is a suppressor | partially of lethal | female limited phenotype of Myc^P1

(Kappes et al., 2011)

sc^sisB-1 is a suppressor | partially of lethal | female limited phenotype of Myc^dm-1

(Kappes et al., 2011)

sc^sisB-1 is a suppressor | partially of lethal | female limited phenotype of Myc^P0

(Kappes et al., 2011)

Other

Statement

Reference

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant | female phenotype

(Gladstein et al., 2010)

msl-3¹, sc[+]/sc^sisB-1 has lethal | female | maternal effect | heat sensitive phenotype

(Gladstein et al., 2010)

sc[+]/sc^sisB-1, sisA¹ has partially lethal - majority die phenotype

(Wrischnik et al., 2003)

sc^sisB-1, sisA[+]/sisA¹ has partially lethal - majority die | dominant phenotype

(Wrischnik et al., 2003)

run³, sc^sisB-1 has lethal | dominant | heat sensitive phenotype

(Duffy and Gergen, 1991)

run², sc^sisB-1 has lethal | dominant | heat sensitive phenotype

(Duffy and Gergen, 1991)

run²², sc^sisB-1 has lethal | dominant | heat sensitive phenotype

(Duffy and Gergen, 1991)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

sc^sisB-1 has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Suppressor of

Statement

Reference

sc^sisB-1 is a suppressor | partially of macrochaeta | ectopic phenotype of hh^bar3

(Wrischnik et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 24% of sc^sisB-1 dm¹ trans-heterozygous female offspring from dm¹ fathers mated to sc^sisB-1 females survive.

Approximately 90% of sc^sisB-1 dm^P0 trans-heterozygous female offspring from dm^P0 fathers mated to sc^sisB-1 mothers survive.

Approximately 76% of sc^sisB-1 dm^P1 trans-heterozygous female offspring from dm^P1 fathers mated to sc^sisB-1 mothers survive.

(Kappes et al., 2011)

sc^sisB-1 sisA¹/+ females show reduced viability compared to their male siblings.

One maternally-derived copy of either mle¹, msl-1^L60, msl-2¹ or msl-3¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females so that they show almost complete lethality. One paternally-derived copy of either mle¹, msl-1^L60, msl-2¹ or msl-3¹ significantly reduces the viability of sc^sisB-1 sisA¹/+ females, although this zygotic effect is generally weaker than the maternal one.

The viability of sc^sisB-1/+ females raised at the non-permissive temperature of 29[o]C is significantly reduced if they also carry one maternally-derived copy of msl-3¹.

One paternally-derived copy of mof², roX1^ex6 or roX2^unspecified reduces the viability of sc^sisB-1 sisA¹/+ females.

The almost complete lethality of sc^sisB-1 sisA¹/+ females carrying a maternally-derived copy of msl-3¹ is significantly rescued (to 72.8% viability) by a maternally-derived copy of Sxl^M1.

(Gladstein et al., 2010)

Ectopic macrochaetae on the scutellum of hh^bar3 homozygotes is only very weakly suppressed by hemizygous sc^sisB-1.

(Wrischnik et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sc^sisB-1/sc^10-1 is rescued by sc^hs.PR

(Torres and Sanchez, 1991)

Partially rescued by

sc^sisB-1 is partially rescued by sc^B6

(Erickson and Cline, 1991)

sc^sisB-1 is partially rescued by sc^B7

(Erickson and Cline, 1991)

sc^sisB-1 is partially rescued by sc^B5

(Erickson and Cline, 1991)

Not rescued by

sc^sisB-1 is not rescued by sc^B23.lacZ

(Erickson and Cline, 1991)

sc^sisB-1 is not rescued by sc^B22

(Erickson and Cline, 1991)

Comments

Rescue statements based on female specific lethality. Bristle phenotype is not rescued by sc⁺ transgenes.

(Erickson and Cline, 1991)

Expression of sc^hs.PR during the first two hours of development rescues female sc^sisB-1/sc^10-1 embryos.

(Torres and Sanchez, 1991)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

106

sc^sisB-1 w^3-1/C(1)DX, y¹ f¹

Kyoto

102030

sc^sisB-1 w¹ / C(1)DX, y¹ f¹

105738

sc^sisB-1 w^3-1/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Sturtevant.

(Lindsley and Zimm, 1992)

Revertant. \partial

(Lindsley and Zimm, 1992)

Comments

The temperature sensitive period begins at nuclear cycle 9 and ends at the beginning of nuclear cycle 14.

(Bopp et al., 1991)

Originally designated "sc^3-1" by Sturtevant, renamed sis-b by Cline.

(Lindsley and Zimm, 1992)

Lethal phenotype is due to failure to properly activate Sxl-Pe.

(Estes et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Df(1)sc^3-1

(Duffy and Gergen, 1991)

sc^3-1

(Kappes et al., 2011, Wrischnik et al., 1997, Deshpande et al., 1995, Lindsley and Zimm, 1992, Bopp et al., 1991, Torres and Sanchez, 1991)

sc^sisB-1

sc^sisB1

(Wrischnik et al., 2003)

sis-b

(Lindsley and Zimm, 1992)

sis-b¹

(Parkhurst and Ish-Horowicz, 1991)

sis-b^3-1

(Parkhurst et al., 1993)

sis-b^sc3-1

(Gladstein et al., 2010, Jinks et al., 2003, Estes et al., 1995, Keyes et al., 1992, Erickson and Cline, 1991)

sisB¹

(Lindsley and Zimm, 1992)

Name Synonyms

sisterless b

(Lindsley and Zimm, 1992)

Secondary FlyBase IDs

FBal0015191

References (25)

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