Somatic MARCM clones of Sce1
homozygous cells in the outer proliferation centre ectopically express Fas3
, suggesting a conversion to inner proliferation centre-like cell identity.
Eye discs composed predominantly of homozygous cells (generated using the FLP/cell-lethal method) are overgrown.
Homozygous neuroblast clones in the third larval instar central brain and ventral ganglion are dramatically reduced in size compared to control clones and none of the mutant clones show mitotic activity, in contrast to control clones.
germline mutant females mated to Sce1
males produce embryos with an identical phenotype as when crossed to Df(3R)IR16
males, suggesting that Sce1
is a null allele.
Somatic mitotic clones of Sce1
are associated with the formation of multicyst egg chambers containing several oocytes, disorganized encapsulation in germarial region 3, and abnormally long interfollicular stalks containing from 10 to over 50 cells. In some cases, a continuous cable of somatic cells can be observed lying along one side of the follicles. These defects are always associated with the presence of mutant cells, but the very few of these cells suffice to create a very abnormal stalk. No excess of polar follicle cells is associated with these clones.
Homozygous embryos derived from homozygous female germ line clones show transformation of all thoracic and abdominal segments into nearly perfect copies of the eighth abdominal segment.
males show weak homeotic phenotypes. No significant interaction is detected in mxcM1
/Y in trans with one mutant copy of Sce1
Pairing sensitive repression is alleviated in iab-7 PRE lines that carry Pc106
Mutation changes the level of w
expression in ph-plac+3
flies; eye colour is darker.
Embryonic lethal or semi-lethal when heterozygous with ph-d2
. Unhatched larvae carry segmentation defects or polarity defects.
A dominant enhancer of Abd-B
in the bithorax complex. Sce1
zygotes from Sce1
/+ mothers die as first instar larvae with weak posteriorly directed transformations, i.e., A7 displays some A8 characteristics. Heterozygous offspring normal. Sce1
embryos from clones of homozygous oocytes produced by pole-cell transplantation display extreme posteriorly directed segmental transformation. Ventral setal belts of all abdominal and thoracic segments transformed toward A8; head involution blocked; abdominal type denticle belts also found anterior to T1 in the presumptive labial and maxillary segments; in addition an extensive belt of abdominal denticles of unknown derivation forms on the anterodorsal surface of the embryo. Keilin's organs and ventral pits suppressed in thoracic segments; wart-like sensilla normally found in A8 formed anteriorally as far as A2. Tracheal branches in A1-7 resemble those normally found more posteriorly. Sce1
/+ offspring from homozygous germ line clones in the mothers may survive to adulthood or die as pharate adults; they show patches of tissue transformed toward A8.
Dominant enhancer of Abd-B. Very weak posteriorly directed transformations.