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General Information
Symbol
Dmel\Sce1
Species
D. melanogaster
Name
FlyBase ID
FBal0015263
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:
410bp deletion removing the codons for the C-terminal 113 Sce amino acids as well as the intron and 12 nucleotides of the 3' untranslated region downstream of the termination codon.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Sce1 homozygous embryos show more extreme homeotic transformations than SceKO or Sce33M2 mutants. This suggests that the truncated protein expressed from the Sce1 not only to lacks Sce[+] function but in addition acts in a dominant-negative fashion.
Deletion of 410bp that removes the codons for the C-terminal 113 amino acids, a small intron and 12 nucleotides of the 3' untranslated region after the termination codon.
410bp deletion removing the codons for the C-terminal 113 Sce amino acids as well as the intron and 12 nucleotides of the 3' untranslated region downstream of the termination codon.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Somatic MARCM clones of Sce1 homozygous cells in the outer proliferation centre ectopically express Fas3, suggesting a conversion to inner proliferation centre-like cell identity.
Eye discs composed predominantly of homozygous cells (generated using the FLP/cell-lethal method) are overgrown.
Homozygous neuroblast clones in the third larval instar central brain and ventral ganglion are dramatically reduced in size compared to control clones and none of the mutant clones show mitotic activity, in contrast to control clones.
Homozygous Sce1/Sce1 germline mutant females mated to Sce1 males produce embryos with an identical phenotype as when crossed to Df(3R)IR16 males, suggesting that Sce1 is a null allele.
Somatic mitotic clones of Sce1 are associated with the formation of multicyst egg chambers containing several oocytes, disorganized encapsulation in germarial region 3, and abnormally long interfollicular stalks containing from 10 to over 50 cells. In some cases, a continuous cable of somatic cells can be observed lying along one side of the follicles. These defects are always associated with the presence of mutant cells, but the very few of these cells suffice to create a very abnormal stalk. No excess of polar follicle cells is associated with these clones.
Sce1/+ suppresses the telomeric position effect (TPE) of P{hsp26-pt-T}39C-5, P{hsp26-pt-T}39C-27, P{hsp26-pt-T}39C-31 and P{wA}4-4.
Homozygous embryos derived from homozygous female germ line clones show transformation of all thoracic and abdominal segments into nearly perfect copies of the eighth abdominal segment.
Hemizygous mxcM1 males show weak homeotic phenotypes. No significant interaction is detected in mxcM1/Y in trans with one mutant copy of Sce1.
Pairing sensitive repression is alleviated in iab-7 PRE lines that carry Pc106 or Sce1.
Mutation changes the level of w expression in ph-plac+3 flies; eye colour is darker.
Embryonic lethal or semi-lethal when heterozygous with ph-d2. Unhatched larvae carry segmentation defects or polarity defects.
Suppression of the Su(z)2hs.PS and Pschs.PS phenotypes.
A dominant enhancer of Abd-B in the bithorax complex. Sce1/Sce1 zygotes from Sce1/+ mothers die as first instar larvae with weak posteriorly directed transformations, i.e., A7 displays some A8 characteristics. Heterozygous offspring normal. Sce1/Sce1 embryos from clones of homozygous oocytes produced by pole-cell transplantation display extreme posteriorly directed segmental transformation. Ventral setal belts of all abdominal and thoracic segments transformed toward A8; head involution blocked; abdominal type denticle belts also found anterior to T1 in the presumptive labial and maxillary segments; in addition an extensive belt of abdominal denticles of unknown derivation forms on the anterodorsal surface of the embryo. Keilin's organs and ventral pits suppressed in thoracic segments; wart-like sensilla normally found in A8 formed anteriorally as far as A2. Tracheal branches in A1-7 resemble those normally found more posteriorly. Sce1/+ offspring from homozygous germ line clones in the mothers may survive to adulthood or die as pharate adults; they show patches of tissue transformed toward A8.
Dominant enhancer of Abd-B. Very weak posteriorly directed transformations.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Sce1 is a suppressor of eye color defective phenotype of wT81
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Sce1 has sex comb | ectopic phenotype, enhanceable by pds5e6
Sce1 has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp1A
Sce1 has sex comb | ectopic phenotype, enhanceable by gpp[+]/gppX
Sce1 has sex comb | ectopic phenotype, enhanceable by gpp[+]/gppXXV
Sce1 has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp8
NOT Enhanced by
Statement
Reference
Sce1 has sex comb | ectopic phenotype, non-enhanceable by pds5e3
Suppressed by
NOT suppressed by
Statement
Reference
Sce1 has sex comb | ectopic phenotype, non-suppressible by Nipped-B407
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Sce[+]/Sce1 is a suppressor | partially of adult mesothoracic segment | ectopic phenotype of E(z)Trm
Sce[+]/Sce1 is a suppressor | partially of adult metathoracic segment phenotype of E(z)Trm
Sce[+]/Sce1 is a suppressor of adult prothoracic segment phenotype of E(z)Trm
Sce1 is a suppressor of phenotype of Su(z)2hs.PS
Sce1 is a suppressor of phenotype of Pschs.PS
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is exacerbated by combination with a single copy of Sce1.
Expression of domeΔCYT.Scer\UAS under the control of Scer\GAL4Act.PU reduces the overgrowth of Sce1 mutant eye discs. Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4Act.PU suppresses the overgrowth of Sce1 mutant eye discs.
The increased apoptosis seen in Scer\GAL4nub-AC-62 RYBPScer\UAS.cBa wing discs is unaffected in a Sce1/+ background.
68% of RYBPKG08683/RYBPKG08683 ; Sce1/+ males show depigmentation of the fifth abdominal segment. 42% of the males have sex combs on the T2 or T3 legs.
No significant modification of the number of sex combs is seen in Sce1/+ corto420/+ double heterozygotes compared to Sce1/+ single heterozygotes.
The addition of heterozygous Sce1 can suppress the Pairing sensitive silencing effect seen in wMcp.10, wMcp.13, wMcp.scs'.12:15, wMcp.scs'.14 and wMcp.y.scs'.1. The addition of heterozygous Sce1 has no effect on the Pairing sensitive silencing effect seen in wMcp.11, wMcp.13, and wMcp.scs'.11. The addition of heterozygous Sce1 has no effect on the Pairing sensitive silencing effect seen in wMcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) or the eye colour of wMcp.scs'.21/ywe.Mcp.1 and wMcp.y.scs'.1/ywe.Mcp.2 double heterozygous insertions. The addition of heterozygous Sce1 strongly suppresses the Pairing sensitive silencing effect seen in wMcp.scs'.14/wMcp.13 (P{Mcp-w#14}AP-2stummelbein/+, P{Mcp-w#13}116/+) and double heterozygous insertions.
Strong suppressor of eye pigment phenotype of wT81.
Embryos doubly mutant for PscArp.1 and Sce1 have ectopic Fell hairs in approximately 30% of cases, and medial erosion of the ventral setal belts (VSBs). The Sce1 phenotype of lack (or partial lack) of the fourth abdominal segment VSB is substantially enhanced.
Xenogenetic Interactions
Statement
Reference
The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is exacerbated by combination with a single copy of Sce1.
Expression of Mmus\Ring1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4prd.RG1 rescues the cuticle phenotype of embryos lacking both maternal and zygotic Sce function (homozygous Sce1 embryos derived from females carrying homozygous Sce1 germ-line clones) in the Scer\GAL4prd.RG1 expression domains.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Silencing of Scer\GAL4-BXD genes is dependent on Pc group gene function, in mutant embryos silencing is lost.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (45)