FlyBase Allele Report: Dmel\Sce[1]

410bp deletion removing the codons for the C-terminal 113 Sce amino acids as well as the intron and 12 nucleotides of the 3' untranslated region downstream of the termination codon.

(Fritsch et al., 2003)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Sce¹ homozygous embryos show more extreme homeotic transformations than Sce^KO or Sce^33M2 mutants. This suggests that the truncated protein expressed from the Sce¹ not only to lacks Sce[+] function but in addition acts in a dominant-negative fashion.

(Gutiérrez et al., 2012)

Deletion of 410bp that removes the codons for the C-terminal 113 amino acids, a small intron and 12 nucleotides of the 3' untranslated region after the termination codon.

(Gorfinkiel et al., 2004)

410bp deletion removing the codons for the C-terminal 113 Sce amino acids as well as the intron and 12 nucleotides of the 3' untranslated region downstream of the termination codon.

(Fritsch et al., 2003)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

decreased cell number | somatic clone

(Bello et al., 2007)

lethal (with Df(3R)IR16)

(Gorfinkiel et al., 2004)

lethal - all die before end of embryonic stage

(McKeon et al., 1994)

lethal - all die before end of larval stage | recessive

(Lindsley and Zimm, 1992)

mitotic cell cycle defective | somatic clone

(Bello et al., 2007)

neuroanatomy defective | somatic clone | third instar larval stage

(Apitz and Salecker, 2016)

some die during embryonic stage

(McKeon et al., 1994)

some die during larval stage | recessive

(Lindsley and Zimm, 1992)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-5

(Boivin et al., 2003)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-27

(Boivin et al., 2003)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-31

(Boivin et al., 2003)

suppressor of variegation | dominant, with P{wA}4-4

(Boivin et al., 2003)

tumorigenic | somatic clone

(Classen et al., 2009)

Phenotype Manifest In

abdominal 4 ventral denticle belt

(Adler et al., 1991)

denticle belt

(Adler et al., 1991)

denticle belt | germline clone

(Lindsley and Zimm, 1992)

egg chamber | somatic clone

(Narbonne et al., 2004)

embryo | embryonic stage 14 | rescuable maternal effect

(Gorfinkiel et al., 2004)

embryonic/larval optic lobe | somatic clone | third instar larval stage

(Apitz and Salecker, 2016)

embryonic/larval tracheal system | germline clone

(Lindsley and Zimm, 1992)

embryonic abdominal segment

(Fritsch et al., 2003)

embryonic abdominal segment 8 | ectopic

(Fritsch et al., 2003)

embryonic abdominal segment 8 | ectopic | rescuable maternal effect

(Gorfinkiel et al., 2004)

embryonic abdominal segment | rescuable maternal effect

(Gorfinkiel et al., 2004)

embryonic head | rescuable maternal effect

(Gorfinkiel et al., 2004)

embryonic thoracic segment

(Fritsch et al., 2003)

eye disc | somatic clone

(Classen et al., 2009)

Fell hair | ectopic

(Adler et al., 1991)

germarium region 3 | somatic clone

(Narbonne et al., 2004)

Keilin's organ | germline clone

(Lindsley and Zimm, 1992)

Kolbchen | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 1 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 2 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 3 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 4 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 5 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 6 | germline clone

(Lindsley and Zimm, 1992)

larval abdominal segment 7

(Lindsley and Zimm, 1992)

larval abdominal segment 7 | germline clone

(Lindsley and Zimm, 1992)

larval mesothoracic segment | germline clone

(Lindsley and Zimm, 1992)

larval metathoracic segment | germline clone

(Lindsley and Zimm, 1992)

larval prothoracic segment | germline clone

(Lindsley and Zimm, 1992)

neuroblast | somatic clone

(Bello et al., 2007)

sex comb | ectopic

(Schaaf et al., 2013)

stalk follicle cell | cell non-autonomous | ectopic | somatic clone

(Narbonne et al., 2004)

Detailed Description

Statement

Reference

Somatic MARCM clones of Sce¹ homozygous cells in the outer proliferation centre ectopically express Fas3, suggesting a conversion to inner proliferation centre-like cell identity.

(Apitz and Salecker, 2016)

Eye discs composed predominantly of homozygous cells (generated using the FLP/cell-lethal method) are overgrown.

(Classen et al., 2009)

Homozygous neuroblast clones in the third larval instar central brain and ventral ganglion are dramatically reduced in size compared to control clones and none of the mutant clones show mitotic activity, in contrast to control clones.

(Bello et al., 2007)

Homozygous Sce¹/Sce¹ germline mutant females mated to Sce¹ males produce embryos with an identical phenotype as when crossed to Df(3R)IR16 males, suggesting that Sce¹ is a null allele.

(Gorfinkiel et al., 2004)

Somatic mitotic clones of Sce¹ are associated with the formation of multicyst egg chambers containing several oocytes, disorganized encapsulation in germarial region 3, and abnormally long interfollicular stalks containing from 10 to over 50 cells. In some cases, a continuous cable of somatic cells can be observed lying along one side of the follicles. These defects are always associated with the presence of mutant cells, but the very few of these cells suffice to create a very abnormal stalk. No excess of polar follicle cells is associated with these clones.

(Narbonne et al., 2004)

Sce¹/+ suppresses the telomeric position effect (TPE) of P{hsp26-pt-T}39C-5, P{hsp26-pt-T}39C-27, P{hsp26-pt-T}39C-31 and P{wA}4-4.

(Boivin et al., 2003)

Homozygous embryos derived from homozygous female germ line clones show transformation of all thoracic and abdominal segments into nearly perfect copies of the eighth abdominal segment.

(Fritsch et al., 2003)

Hemizygous mxc^M1 males show weak homeotic phenotypes. No significant interaction is detected in mxc^M1/Y in trans with one mutant copy of Sce¹.

(Saget et al., 1998)

Pairing sensitive repression is alleviated in iab-7 PRE lines that carry Pc¹⁰⁶ or Sce¹.

(Hagstrom et al., 1997)

Mutation changes the level of w expression in ph-p^lac+3 flies; eye colour is darker.

(Fauvarque et al., 1995)

Embryonic lethal or semi-lethal when heterozygous with ph-d². Unhatched larvae carry segmentation defects or polarity defects.

(McKeon et al., 1994)

Suppression of the Su(z)2^hs.PS and Psc^hs.PS phenotypes.

(Sharp et al., 1994)

A dominant enhancer of Abd-B in the bithorax complex. Sce¹/Sce¹ zygotes from Sce¹/+ mothers die as first instar larvae with weak posteriorly directed transformations, i.e., A7 displays some A8 characteristics. Heterozygous offspring normal. Sce¹/Sce¹ embryos from clones of homozygous oocytes produced by pole-cell transplantation display extreme posteriorly directed segmental transformation. Ventral setal belts of all abdominal and thoracic segments transformed toward A8; head involution blocked; abdominal type denticle belts also found anterior to T1 in the presumptive labial and maxillary segments; in addition an extensive belt of abdominal denticles of unknown derivation forms on the anterodorsal surface of the embryo. Keilin's organs and ventral pits suppressed in thoracic segments; wart-like sensilla normally found in A8 formed anteriorally as far as A2. Tracheal branches in A1-7 resemble those normally found more posteriorly. Sce¹/+ offspring from homozygous germ line clones in the mothers may survive to adulthood or die as pharate adults; they show patches of tissue transformed toward A8.

(Lindsley and Zimm, 1992)

Dominant enhancer of Abd-B. Very weak posteriorly directed transformations.

(Breen and Duncan, 1986)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sce¹ has tumorigenic | somatic clone phenotype, suppressible | somatic clone by dome^ΔCYT.UAS/Scer\GAL4^Act.PU

(Classen et al., 2009)

Sce¹ has tumorigenic | somatic clone phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Socs36E^UASp.cCa

(Classen et al., 2009)

Enhancer of

Statement

Reference

Sce[+]/Sce¹ is an enhancer of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

Sce[+]/Sce¹ is an enhancer of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Suppressor of

Statement

Reference

Sce¹ is a suppressor | partially of eye color defective phenotype of w^ph-p.lac+3/w¹¹¹⁸

(Bloyer et al., 2003)

Sce¹ is a suppressor of eye color defective phenotype of w^T81

(Netter et al., 1998)

NOT Suppressor of

Statement

Reference

Sce[+]/Sce¹ is a non-suppressor of increased cell death phenotype of RYBP^UAS.cBa, Scer\GAL4^nub-AC-62

(González and Busturia, 2009)

Other

Statement

Reference

RYBP^KG08683, Sce¹ has visible | dominant phenotype

(González et al., 2008)

Sce¹, ph-d² has lethal | embryonic stage phenotype

(McKeon et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sce¹ has sex comb | ectopic phenotype, enhanceable by pds5^e6

(Schaaf et al., 2013)

Sce¹ has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp^1A

(Shanower et al., 2005)

Sce¹ has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp^X

(Shanower et al., 2005)

Sce¹ has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp^XXV

(Shanower et al., 2005)

Sce¹ has sex comb | ectopic phenotype, enhanceable by gpp[+]/gpp⁸

(Shanower et al., 2005)

Sce¹ has abdominal 4 ventral denticle belt phenotype, enhanceable by Psc^Arp.1

(Adler et al., 1991)

NOT Enhanced by

Statement

Reference

Sce¹ has sex comb | ectopic phenotype, non-enhanceable by pds5^e3

(Schaaf et al., 2013)

Suppressed by

Statement

Reference

Sce¹ has sex comb | ectopic phenotype, suppressible | partially by vtd⁶

(Schaaf et al., 2013)

Sce¹ has sex comb | ectopic phenotype, suppressible | partially by vtd^80Fh-36

(Schaaf et al., 2013)

Sce¹ has sex comb | ectopic phenotype, suppressible by SMC1^exc46

(Schaaf et al., 2013)

Sce¹ has eye disc | somatic clone phenotype, suppressible | somatic clone by dome^ΔCYT.UAS/Scer\GAL4^Act.PU

(Classen et al., 2009)

Sce¹ has eye disc | somatic clone phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Socs36E^UASp.cCa

(Classen et al., 2009)

Sce¹ has neuroblast | somatic clone phenotype, suppressible by Scer\GAL4^αTub84B.PL/BacA\p35^UAS.cHa

(Bello et al., 2007)

Sce¹ has sex comb | ectopic phenotype, suppressible by gpp[+]/gpp^94A

(Shanower et al., 2005)

Sce¹ has embryonic abdominal segment | rescuable maternal effect phenotype, suppressible | partially by Scer\GAL4^prd.RG1/Mmus\Ring1^UAS.Tag:MYC

(Gorfinkiel et al., 2004)

NOT suppressed by

Statement

Reference

Sce¹ has sex comb | ectopic phenotype, non-suppressible by Nipped-B⁴⁰⁷

(Schaaf et al., 2013)

Enhancer of

Statement

Reference

Sce[+]/Sce¹ is an enhancer of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

Sce[+]/Sce¹ is an enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Suppressor of

Statement

Reference

Sce[+]/Sce¹ is a suppressor | partially of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

Sce[+]/Sce¹ is a suppressor | partially of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Sce[+]/Sce¹ is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Sce¹ is a suppressor of phenotype of Su(z)2^hs.PS

(Sharp et al., 1994)

Sce¹ is a suppressor of phenotype of Psc^hs.PS

(Sharp et al., 1994)

NOT Suppressor of

Statement

Reference

Sce[+]/Sce¹ is a non-suppressor of wing disc | third instar larval stage phenotype of RYBP^UAS.cBa, Scer\GAL4^nub-AC-62

(González and Busturia, 2009)

Other

Statement

Reference

RYBP^KG08683, Sce¹ has adult abdominal segment 5 phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has sex comb | ectopic phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has mesothoracic leg phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has metathoracic leg phenotype

(González et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is exacerbated by combination with a single copy of Sce¹.

(Morimoto et al., 2016)

Expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^Act.PU reduces the overgrowth of Sce¹ mutant eye discs.

Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^Act.PU suppresses the overgrowth of Sce¹ mutant eye discs.

(Classen et al., 2009)

The increased apoptosis seen in Scer\GAL4^nub-AC-62 RYBP^Scer\UAS.cBa wing discs is unaffected in a Sce¹/+ background.

(González and Busturia, 2009)

68% of RYBP^KG08683/RYBP^KG08683 ; Sce¹/+ males show depigmentation of the fifth abdominal segment. 42% of the males have sex combs on the T2 or T3 legs.

(González et al., 2008)

No significant modification of the number of sex combs is seen in Sce¹/+ corto⁴²⁰/+ double heterozygotes compared to Sce¹/+ single heterozygotes.

(Lopez et al., 2001)

The addition of heterozygous Sce¹ can suppress the Pairing sensitive silencing effect seen in w^Mcp.10, w^Mcp.13, w^{Mcp.scs'.12:15}, w^Mcp.scs'.14 and w^Mcp.y.scs'.1. The addition of heterozygous Sce¹ has no effect on the Pairing sensitive silencing effect seen in w^Mcp.11, w^Mcp.13, and w^Mcp.scs'.11. The addition of heterozygous Sce¹ has no effect on the Pairing sensitive silencing effect seen in w^Mcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) or the eye colour of w^Mcp.scs'.21/y^we.Mcp.1 and w^Mcp.y.scs'.1/y^we.Mcp.2 double heterozygous insertions. The addition of heterozygous Sce¹ strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.scs'.14/w^Mcp.13 (P{Mcp-w#14}AP-2^stummelbein/+, P{Mcp-w#13}116/+) and double heterozygous insertions.

(Muller et al., 1999)

Strong suppressor of eye pigment phenotype of w^T81.

(Netter et al., 1998)

Embryos doubly mutant for Psc^Arp.1 and Sce¹ have ectopic Fell hairs in approximately 30% of cases, and medial erosion of the ventral setal belts (VSBs). The Sce¹ phenotype of lack (or partial lack) of the fourth abdominal segment VSB is substantially enhanced.

(Adler et al., 1991)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is exacerbated by combination with a single copy of Sce¹.

(Morimoto et al., 2016)

Expression of Mmus\Ring1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^prd.RG1 rescues the cuticle phenotype of embryos lacking both maternal and zygotic Sce function (homozygous Sce¹ embryos derived from females carrying homozygous Sce¹ germ-line clones) in the Scer\GAL4^prd.RG1 expression domains.

(Gorfinkiel et al., 2004)

Complementation and Rescue Data

Fails to complement

Sce^33M2

(Fritsch et al., 2003)

Rescued by

Df(3R)BSC499/Sce¹ is rescued by Scer\GAL4^arm.PS/Sce^{UAS.C.Tag:FLAG}

(Langlais et al., 2012)

Sce¹ is rescued by Sce^UAS.Tag:MYC/Scer\GAL4^arm.PS

(Gorfinkiel et al., 2004)

Partially rescued by

Sce¹ is partially rescued by Scer\GAL4^prd.RG1/Sce^UAS.Tag:MYC

(Gorfinkiel et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24618

w^*; P{neoFRT}82B Abd-B^Mcp-1 Sce¹/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

Comments

Silencing of Scer\GAL4-BXD genes is dependent on Pc group gene function, in mutant embryos silencing is lost.

(Muller, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Sce¹

(Leatham-Jensen et al., 2019, Dardalhon-Cuménal et al., 2018, Apitz and Salecker, 2016, Bivik et al., 2015, Schaaf et al., 2013, Gutiérrez et al., 2012, Ji et al., 2012, Langlais et al., 2012, Li et al., 2010, Scheuermann et al., 2010, González and Busturia, 2009, González et al., 2008, Bello et al., 2007, de Ayala Alonso et al., 2007, Bejarano et al., 2005, Adler et al., 1991)

Name Synonyms

Secondary FlyBase IDs

References (46)

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