FlyBase Allele Report: Dmel\Scm[4]

General Information

Symbol

Dmel\Scm⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0015268

Feature type

allele

Associated gene

Dmel\Scm

Associated Insertion(s)

Carried in Construct

Also Known As

Scm^XF24

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Bornemann et al., 1998, Bornemann et al., 1996)

Mutagen

X ray

(Bornemann et al., 1998, Bornemann et al., 1996, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Deletion of the C-terminal 49 amino acids.

(Bornemann et al., 1998)

Approximately 200bp deletion, plus insertion of 4 nucleotides (CTGA), within the 3' region of the Scm transcription unit, at the 3' end of the ORF. The C-terminal 49 amino acids of Scm protein, including most of the SPM domain, are removed and replaced by 26 novel amino acids encoded by extension of the open reading frame into the 3' UTR.

(Bornemann et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:9,634,297..9,634,547 [-]

Comment:

251bp at the 3' end of Scm are deleted and replaced with 4 nucleotides (CTGA). As a result, the final 49 amino acids of Scm are deleted and replaced with 26 novel amino acids.

Inserted_sequence:

CTGA

(Bornemann et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Bornemann et al., 1998, Wu et al., 1989)

lethal - all die during embryonic stage (with Scm^H1)

(Peterson et al., 2004)

some die during embryonic stage (with Scm^H1)

(Peterson et al., 2004)

Phenotype Manifest In

sex comb | ectopic

(Wu et al., 1989)

Detailed Description

Statement

Reference

Homozygotes die during the embryonic or first larval instar stages.

(Bornemann et al., 1998)

Strong allele.

(Bornemann et al., 1996)

Pc³ Scm⁴ heterozygotes have an extreme wing to haltere transformation. This phenotype is normalised towards wild-type in flies also carrying a Ubx mutation in the coding region or abx regulatory region.

(Castelli-Gair and Garcia-Bellido, 1990)

Heterozygous males are viable and often show ectopic sex comb teeth. Mild suppressor of z¹ eye colour.

(Wu et al., 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scm⁴ is an enhancer of visible | dominant | homeotic phenotype of Pc³

(Bornemann et al., 1998)

Suppressor of

Statement

Reference

Scm⁴/Scm[+] is a suppressor of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Other

Statement

Reference

Scm⁴, ph-p⁴⁰⁹ has partially lethal phenotype

(Bornemann et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scm⁴ is an enhancer of wing disc | ectopic | somatic clone phenotype of wg^{αTub84B.Tag:HA}

(Lee et al., 2005)

Scm⁴ is an enhancer of leg disc | somatic clone phenotype of wg^{αTub84B.Tag:HA}

(Lee et al., 2005)

Suppressor of

Statement

Reference

Scm⁴/Scm[+] is a suppressor of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Scm⁴/Scm[+] is a suppressor of wing vein | increased number phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^cv-c-C5

(Cukier et al., 2008)

Scm⁴/Scm[+] is a suppressor of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Scm⁴ is a suppressor of phenotype of z¹

(Bornemann et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

No significant modification of the number of sex combs is seen in Scm⁴/+ corto⁴²⁰/+ double heterozygotes compared to Scm⁴/+ single heterozygotes.

(Lopez et al., 2001)

Dominantly enhances the homeotic phenotypes of Pc³/+ adults. ph-p⁴⁰⁹ males that are also heterozygous for Scm⁴ show partial lethality.

(Bornemann et al., 1998)

Xenogenetic Interactions

Statement

Reference

The addition of Scm⁴ increases the rate of leg to wing disc transdetermination to 11.0%

(Lee et al., 2005)

Complementation and Rescue Data

Fails to complement

Rescued by

Scm⁴/Scm^H1 is rescued by Scm^{triple.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is rescued by Scm^WT.Tag:FLAG

(Peterson et al., 2004)

Partially rescued by

Scm^ET50/Scm⁴ is partially rescued by Scm^Cas-Scm7

(Bornemann et al., 1996)

Not rescued by

Scm⁴/Scm^H1 is not rescued by Scm^{ΔPSM.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is not rescued by Scm^{I29T.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is not rescued by Scm^{M46R.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is not rescued by Scm^{G31D.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is not rescued by Scm^{M43Δ.Tag:FLAG}

(Peterson et al., 2004)

Scm⁴/Scm^H1 is not rescued by Scm^{K55E.Tag:FLAG}

(Peterson et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Weak derepression of en expression.

(Moazed and O'Farrell, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scm XF24

(Cukier et al., 2008)

Scm⁴

Scm^XF24

(Lee et al., 2005, Peterson et al., 2004, Belenkaya et al., 1998, Bornemann et al., 1998, Bornemann et al., 1996, Castelli-Gair and Garcia-Bellido, 1990, Wu et al., 1989, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (12)

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