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General Information
Symbol
Dmel\Scr4
Species
D. melanogaster
Name
FlyBase ID
FBal0015280
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Scrw17, ScreW17
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C6841790T

Reported nucleotide change:

C393T

Amino acid change:

Q131term | Scr-PA; Q131term | Scr-PB; Q131term | Scr-PD; Q131term | Scr-PE; Q131term | Scr-PF

Reported amino acid change:

Q131term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Nucleotide substitution: C393T.

Amino acid replacement: Q131term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mesothoracic leg & sex comb | ectopic (with ScrE2), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrMsc), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrP), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrRpl), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrScx)

mesothoracic leg & sex comb | ectopic (with ScrScx), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrT3), with Dp(3;Y)77ab

mesothoracic leg & sex comb | ectopic (with ScrW)

mesothoracic leg & sex comb | ectopic (with ScrW), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrMsc), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrP), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrRpl), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrScx)

metathoracic leg & sex comb | ectopic (with ScrScx), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrT2), with Dp(3;Y)77ab

metathoracic leg & sex comb | ectopic (with ScrW)

Detailed Description
Statement
Reference

Scr4 mutant embryos lack prothoracic glands.

Heterozygous males have an average of 6.2 sex comb teeth in the T1 leg.

ScrW Scr4/+ males (mutations in cis) have an average of 4.5 sex comb teeth in the T1 leg.

Heterozygous males have a reduction in the number of sex comb bristles on the prothoracic leg compared to wild-type males.

Scr4/+ males show a reduction in the number of sex comb teeth on the first leg compared to wild type.

Heterozygous males have a reduced number of sex comb teeth on the first leg (average is 6.2 per leg in these animals). Heterozygous males

have no ectopic sex comb teeth on the second or third legs.

Scr4/ScrAntp-A41 males are recovered at 1% of expected frequency

and rarely have sex comb teeth on the first legs (average is 0.08 per

leg in these animals).

Scr4/ScrHu males are recovered at 2% of expected frequency

and do not have sex comb teeth on the first legs.

Scr4/ScrTpl9; Dp(3;Y)77ab males (in which the haplolethality

of the Df(3R)Tpl9 chromosome has been rescued by Dp(3;3)Dfdrv1)

have an average of 6.4 sex comb teeth per first leg (compared to 10.8

per leg in wild type).

Scr4/Df(3R)Scr; Dp(3;Y)77ab males have an average of 5.9 sex

comb teeth per first leg (compared to 10.8 per leg in wild type).

Scr4/ScrLIN; Dp(3;Y)77ab males have an average of 7.0 sex

comb teeth per first leg (compared to 10.8 per leg in wild type).

ScrScx/Scr4 males have the same average number of sex comb

teeth per first leg as Scr4/+ males.

Scr4/ScrWrv4 males have an average of 0.04 sex comb teeth on

the first leg (compared to 10.8 per leg in wild type).

ScrMsc/Scr4 ; Dp(3;Y)77ab males have an average of 0.3 ectopic

sex comb teeth per second leg.

ScrMsc/Scr4 ; Dp(3;Y)77ab males have an average of 0.1 ectopic

sex comb teeth per third leg.

ScrScx/Scr4 males have an average of 1.6 ectopic sex comb teeth

per second leg and an average of 0.1 ectopic sex comb teeth per third

leg.

ScrScx/Scr4; Dp(3;Y)77ab males have an average of 1.4 ectopic

sex comb teeth per second leg and an average of 0.2 ectopic sex comb

teeth per third leg.

ScrE2/Scr4 ; Dp(3;Y)77ab males (ScrE2 chromosome carries

the "E2" mutation in cis to ScrScx) have an average of 0.02 ectopic

sex comb teeth per second leg and have no ectopic sex comb teeth on

the third legs.

Scr9/Scr4 ; Dp(3;Y)77ab males have an average of 0.002 ectopic

sex comb teeth per second leg and have no ectopic sex comb teeth on

the third legs.

ScrT1/Scr4 ; Dp(3;Y)77ab males have no ectopic sex comb teeth

on the second or third legs.

ScrT2/Scr4 ; Dp(3;Y)77ab males have an average of 0.01 ectopic

sex comb teeth per third leg and have no ectopic sex comb teeth on

the second legs.

ScrRpl/Scr4; Dp(3;Y)77ab males have an average of 0.03 ectopic

sex comb teeth per second leg and an average of 0.02 ectopic sex comb

teeth per third leg.

ScrP/Scr4; Dp(3;Y)77ab males have an average of 0.3 ectopic

sex comb teeth per second leg and an average of 0.03 ectopic sex comb

teeth per third leg.

ScrT3/Scr4; Dp(3;Y)77ab males have an average of 0.03 ectopic

sex comb teeth per second leg and no ectopic sex comb teeth on the

third legs.

ScrW/Scr4 males (mutations in trans) have an average of 0.1

ectopic sex comb teeth per second leg and no ectopic sex comb teeth

on the third legs.

ScrW Scr4/+ males (mutations in cis) have an average of 3.2

ectopic sex comb teeth per second leg and an average of 1.0 ectopic

sex comb teeth per third leg.

ScrW/Scr4 ; Dp(3;Y)77ab males have an average of 0.1 ectopic

sex comb teeth per second leg and no ectopic sex comb teeth on the

third legs.

The sex comb on the first tarsal segment of the prothoracic leg is approximately 50% reduced in heterozygous males.

Homozygous embryos do not develop salivary glands.

Scr4/Df(3R)Scr embryonic labial lobes do not migrate ventrally or enter the stomodeum, the sensory organs produced by these lobes remain external to become ectopic external sensory organs. Contrary to the report of FBrf0054155, ectopic cirri and mouth hooks are not generally produced by the mutant labial lobes of Scr4/Df(3R)Scr embryos. Dfd16 Scr4 double homozygous embryos exhibit failure of the labial lobes to migrate ventrally or internalise. Ectopic external sensory organs derived from failure of the labial lobes to internalise are the same as for Scr- embryos.

Staining with antibody shows a T1 pattern of es organs. Scr4 is required for lch3 morphology.

Salivary glands are absent at all stages of development.

No change in the spatial distribution of ae mRNA. Scr4/tsh8 double mutants develop the dorsala part of the thoracic trunk segment, whereas tsh8 mutants do not.

Homozygotes lack embryonic expression of the protein and fail to form gastric caeca.

Thoracic segment 1 is transformed to thoracic segment 2 and labial is transformed to maxillary.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Scr4 has visible phenotype, enhanceable by Dsp11

Enhancer of
Statement
Reference

Scr4/Scr[+] is an enhancer of visible | homeotic phenotype of Dsp11

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scr4 has sex comb tooth phenotype, enhanceable by Df(2R)ED3921

Scr4 has sex comb phenotype, enhanceable by Dsp11

Enhancer of
Statement
Reference

Scr4/Scr[+] is an enhancer of sex comb phenotype of Dsp11

Suppressor of
Statement
Reference

Scr4 is a suppressor of prothoracic leg | distal phenotype of pbhs.PB

Scr4 is a suppressor of sex comb | ectopic phenotype of pbhs.PB

Scr4 is a suppressor of prothoracic leg | distal phenotype of pbhs.PB, skdpap-53

Scr4 is a suppressor of sex comb | ectopic phenotype of pbhs.PB, skdpap-53

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

In Dfd16;Scr4 double mutant embryos the corpus allata and prothoracic glands do not form.

Addition of DfdScer\UAS.cBa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming corpora allata.

Addition of ScrScer\UAS.cMa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming prothoracic glands.

Addition of UbxScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Addition of Abd-BScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Addition of AntpScer\UAS.cMb to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Abd-BFab7-12 (which deletes the Fab-7 element) induces a significant increase in the number of sex comb teeth in the T1 leg in ScrW Scr4/+ males (mutations in cis).

Df(2R)ED3921; Scr4/+ double mutants show a greater reduction in sex comb teeth than Scr4/+ single mutants.

Scr4 Antp25 embryos show a wild-type oenocyte pattern.

Scr4/Df(3R)Antp7 animals show 11% viability. Scr4/Df(3R)Antp7 males have an average of 5.6 sex comb teeth on the first leg.

pb1 Scr5/pb5 Scr4 flies show transformation of the proboscis into the antenna. The transformation varies from thick and shortened aristae with claws and pulvillae to apparently complete antennae with aristae, third and second antennal segments bearing characteristic bristles.

Dsp11/Y ; Scr4/+ males show an increase in the severity of the phenotype; the size of the sex comb teeth is greatly reduced, with an average number of 4 teeth in the double mutant versus either single mutant combination.

The addition of Scr4 to pbhs.PB, skdpap-53 flies suppresses the distal sex comb phenotype seen in these animals. MED17s2956, ScrP, skdpap-53 flies exhibit a stronger ectopic distal sex comb phenotype than any of the double mutant combinations.

scd1/Y Scr4/+ males lack the small distal sex comb found in scd1/Y males, but the sex comb on the first tarsal segment of the leg contains more teeth than the sex comb of Scr4/+ heterozygous males.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer

Wakimoto.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (53)