FB2025_01 , released February 20, 2025
Allele: Dmel\Scr4
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General Information
Symbol
Dmel\Scr4
Species
D. melanogaster
Name
FlyBase ID
FBal0015280
Feature type
allele
Associated gene
Dmel\Scr
Associated Insertion(s)
Carried in Construct
Also Known As
Scrw17, ScreW17
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Sivanantharajah and Percival-Smith, 2009, Gorman and Kaufman, 1995, McCormick et al., 1995, Pattatucci and Kaufman, 1991, Reuter and Scott, 1990, Reuter et al., 1990)

loss of function allele

(Henderson and Andrew, 2000, Heuer and Kaufman, 1992, Pattatucci et al., 1991)
Mutagen
ethyl methanesulfonate
(Pattatucci et al., 1991, Lewis et al., 1980)
Progenitor genotype
Cytology

Polytene chromosomes normal.

(Pattatucci et al., 1991)
Description

Nucleotide substitution: C393T.

(Sivanantharajah and Percival-Smith, 2009)

Amino acid replacement: Q131term.

(Sivanantharajah and Percival-Smith, 2009)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:6,841,790..6,841,790 [-]
Nucleotide change:

C6841790T

Reported nucleotide change:

C393T

Amino acid change:

Q131term | Scr-PA; Q131term | Scr-PB; Q131term | Scr-PD; Q131term | Scr-PE; Q131term | Scr-PF

Reported amino acid change:

Q131term

(Sivanantharajah and Percival-Smith, 2009)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      embryonic primordium of adult salivary gland

      (Jullien et al., 1997, Panzer et al., 1992)

      labial disc

      (Pederson et al., 1996)

      larval labial organ

      (Pederson et al., 1996)

      mesothoracic leg (with ScrE2), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrMsc), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrP), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrRpl), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrScx)

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrScx), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrT3), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      mesothoracic leg (with ScrW), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrMsc), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrP), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrRpl), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrScx)

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrScx), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      metathoracic leg (with ScrT2), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      prothoracic gland

      (Sánchez-Higueras et al., 2014)

      pseudotrachea (with Scr14)

      (Sivanantharajah and Percival-Smith, 2009)

      sex comb

      (Sivanantharajah and Percival-Smith, 2009, Southworth and Kennison, 2002, Boube et al., 1997)

      sex comb (with Df(3R)Scr), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb (with Scr14)

      (Sivanantharajah and Percival-Smith, 2009)

      sex comb (with ScrAntp-A41)

      (Southworth and Kennison, 2002)

      sex comb (with ScrHu)

      (Southworth and Kennison, 2002)

      sex comb (with ScrLIN), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb (with ScrWrv4)

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrE2), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrMsc), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrP), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrRpl), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrScx)

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrScx), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrT2), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrT3), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrTpl9), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrW)

      (Southworth and Kennison, 2002)

      sex comb | ectopic (with ScrW), with Dp(3;Y)77ab

      (Southworth and Kennison, 2002)

      sex comb tooth

      (Bantignies et al., 2011, Ragab et al., 2006)

      sex comb tooth (with ScrW)

      (Bantignies et al., 2011)
      Detailed Description
      Statement
      Reference

      Scr4 mutant embryos lack prothoracic glands.

      (Sánchez-Higueras et al., 2014)

      Heterozygous males have an average of 6.2 sex comb teeth in the T1 leg.

      ScrW Scr4/+ males (mutations in cis) have an average of 4.5 sex comb teeth in the T1 leg.

      (Bantignies et al., 2011)

      Heterozygous males have a reduction in the number of sex comb bristles on the prothoracic leg compared to wild-type males.

      (Sivanantharajah and Percival-Smith, 2009)

      Scr4/+ males show a reduction in the number of sex comb teeth on the first leg compared to wild type.

      (Ragab et al., 2006)

      Heterozygous males have a reduced number of sex comb teeth on the first leg (average is 6.2 per leg in these animals). Heterozygous males

      have no ectopic sex comb teeth on the second or third legs.

      Scr4/ScrAntp-A41 males are recovered at 1% of expected frequency

      and rarely have sex comb teeth on the first legs (average is 0.08 per

      leg in these animals).

      Scr4/ScrHu males are recovered at 2% of expected frequency

      and do not have sex comb teeth on the first legs.

      Scr4/ScrTpl9; Dp(3;Y)77ab males (in which the haplolethality

      of the Df(3R)Tpl9 chromosome has been rescued by Dp(3;3)Dfdrv1)

      have an average of 6.4 sex comb teeth per first leg (compared to 10.8

      per leg in wild type).

      Scr4/Df(3R)Scr; Dp(3;Y)77ab males have an average of 5.9 sex

      comb teeth per first leg (compared to 10.8 per leg in wild type).

      Scr4/ScrLIN; Dp(3;Y)77ab males have an average of 7.0 sex

      comb teeth per first leg (compared to 10.8 per leg in wild type).

      ScrScx/Scr4 males have the same average number of sex comb

      teeth per first leg as Scr4/+ males.

      Scr4/ScrWrv4 males have an average of 0.04 sex comb teeth on

      the first leg (compared to 10.8 per leg in wild type).

      ScrMsc/Scr4 ; Dp(3;Y)77ab males have an average of 0.3 ectopic

      sex comb teeth per second leg.

      ScrMsc/Scr4 ; Dp(3;Y)77ab males have an average of 0.1 ectopic

      sex comb teeth per third leg.

      ScrScx/Scr4 males have an average of 1.6 ectopic sex comb teeth

      per second leg and an average of 0.1 ectopic sex comb teeth per third

      leg.

      ScrScx/Scr4; Dp(3;Y)77ab males have an average of 1.4 ectopic

      sex comb teeth per second leg and an average of 0.2 ectopic sex comb

      teeth per third leg.

      ScrE2/Scr4 ; Dp(3;Y)77ab males (ScrE2 chromosome carries

      the "E2" mutation in cis to ScrScx) have an average of 0.02 ectopic

      sex comb teeth per second leg and have no ectopic sex comb teeth on

      the third legs.

      Scr9/Scr4 ; Dp(3;Y)77ab males have an average of 0.002 ectopic

      sex comb teeth per second leg and have no ectopic sex comb teeth on

      the third legs.

      ScrT1/Scr4 ; Dp(3;Y)77ab males have no ectopic sex comb teeth

      on the second or third legs.

      ScrT2/Scr4 ; Dp(3;Y)77ab males have an average of 0.01 ectopic

      sex comb teeth per third leg and have no ectopic sex comb teeth on

      the second legs.

      ScrRpl/Scr4; Dp(3;Y)77ab males have an average of 0.03 ectopic

      sex comb teeth per second leg and an average of 0.02 ectopic sex comb

      teeth per third leg.

      ScrP/Scr4; Dp(3;Y)77ab males have an average of 0.3 ectopic

      sex comb teeth per second leg and an average of 0.03 ectopic sex comb

      teeth per third leg.

      ScrT3/Scr4; Dp(3;Y)77ab males have an average of 0.03 ectopic

      sex comb teeth per second leg and no ectopic sex comb teeth on the

      third legs.

      ScrW/Scr4 males (mutations in trans) have an average of 0.1

      ectopic sex comb teeth per second leg and no ectopic sex comb teeth

      on the third legs.

      ScrW Scr4/+ males (mutations in cis) have an average of 3.2

      ectopic sex comb teeth per second leg and an average of 1.0 ectopic

      sex comb teeth per third leg.

      ScrW/Scr4 ; Dp(3;Y)77ab males have an average of 0.1 ectopic

      sex comb teeth per second leg and no ectopic sex comb teeth on the

      third legs.

      (Southworth and Kennison, 2002)

      The sex comb on the first tarsal segment of the prothoracic leg is approximately 50% reduced in heterozygous males.

      (Boube et al., 1997)

      Homozygous embryos do not develop salivary glands.

      (Jullien et al., 1997)

      Scr4/Df(3R)Scr embryonic labial lobes do not migrate ventrally or enter the stomodeum, the sensory organs produced by these lobes remain external to become ectopic external sensory organs. Contrary to the report of FBrf0054155, ectopic cirri and mouth hooks are not generally produced by the mutant labial lobes of Scr4/Df(3R)Scr embryos. Dfd16 Scr4 double homozygous embryos exhibit failure of the labial lobes to migrate ventrally or internalise. Ectopic external sensory organs derived from failure of the labial lobes to internalise are the same as for Scr- embryos.

      (Pederson et al., 1996)

      Staining with antibody shows a T1 pattern of es organs. Scr4 is required for lch3 morphology.

      (Heuer and Kaufman, 1992)

      Salivary glands are absent at all stages of development.

      (Panzer et al., 1992)

      No change in the spatial distribution of ae mRNA. Scr4/tsh8 double mutants develop the dorsala part of the thoracic trunk segment, whereas tsh8 mutants do not.

      (Roder et al., 1992)

      Homozygotes lack embryonic expression of the protein and fail to form gastric caeca.

      (Reuter and Scott, 1990)

      Thoracic segment 1 is transformed to thoracic segment 2 and labial is transformed to maxillary.

      (Jack et al., 1988)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scr4 has visible phenotype, enhanceable by Dsp11

      (Decoville et al., 2001)
      Enhancer of
      Statement
      Reference

      Scr4/Scr[+] is an enhancer of visible | homeotic phenotype of Dsp11

      (Decoville et al., 2001)
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scr4 has sex comb tooth phenotype, enhanceable by Df(2R)ED3921

      (Ragab et al., 2006)

      Scr4 has sex comb phenotype, enhanceable by Dsp11

      (Decoville et al., 2001)
      Enhancer of
      Statement
      Reference

      Scr4/Scr[+] is an enhancer of sex comb phenotype of Dsp11

      (Decoville et al., 2001)
      Suppressor of
      Statement
      Reference

      Scr4 is a suppressor of prothoracic leg | distal phenotype of pbhs.PB, skdpap-53

      (Boube et al., 2000)

      Scr4 is a suppressor of sex comb | ectopic phenotype of pbhs.PB, skdpap-53

      (Boube et al., 2000)

      Scr4 is a suppressor of prothoracic leg | distal phenotype of pbhs.PB

      (Boube et al., 2000)

      Scr4 is a suppressor of sex comb | ectopic phenotype of pbhs.PB

      (Boube et al., 2000)
      Other
      Statement
      Reference

      Dfd16, Scr4 has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, Scr4 has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, Scer\GAL4salm-459.2, Scr4, ScrUAS.cMa has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, DfdUAS.cBa, Scer\GAL4salm-459.2, Scr4 has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, DfdUAS.cBa, Scer\GAL4salm-459.2, Scr4 has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, Scer\GAL4salm-459.2, Scr4, ScrUAS.cMa has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, Scer\GAL4salm-459.2, Scr4, UbxUAS.cCa has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      Dfd16, Scer\GAL4salm-459.2, Scr4, UbxUAS.cCa has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      Abd-BUAS.cCa, Dfd16, Scer\GAL4salm-459.2, Scr4 has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      Abd-BUAS.cCa, Dfd16, Scer\GAL4salm-459.2, Scr4 has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      AntpUAS.cMb, Dfd16, Scer\GAL4salm-459.2, Scr4 has adult corpus allatum phenotype

      (Sánchez-Higueras et al., 2014)

      AntpUAS.cMb, Dfd16, Scer\GAL4salm-459.2, Scr4 has prothoracic gland phenotype

      (Sánchez-Higueras et al., 2014)

      Scr4/Scr5, pb5/pb1 has proboscis phenotype

      (Abzhanov et al., 2001)

      Scr4/Scr5, pb5/pb1 has antenna | ectopic phenotype

      (Abzhanov et al., 2001)

      Scr4/Scr5, pb5/pb1 has arista | ectopic phenotype

      (Abzhanov et al., 2001)

      Scr4/Scr5, pb5/pb1 has third segment of antenna | ectopic phenotype

      (Abzhanov et al., 2001)

      Scr4/Scr5, pb5/pb1 has second segment of antenna | ectopic phenotype

      (Abzhanov et al., 2001)

      Scr4, scd1 has sex comb phenotype

      (Boube et al., 1997)

      Scr4, scd1 has prothoracic tarsal segment 2 phenotype

      (Boube et al., 1997)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In Dfd16;Scr4 double mutant embryos the corpus allata and prothoracic glands do not form.

      Addition of DfdScer\UAS.cBa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming corpora allata.

      Addition of ScrScer\UAS.cMa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming prothoracic glands.

      Addition of UbxScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

      Addition of Abd-BScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

      Addition of AntpScer\UAS.cMb to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

      (Sánchez-Higueras et al., 2014)

      Abd-BFab7-12 (which deletes the Fab-7 element) induces a significant increase in the number of sex comb teeth in the T1 leg in ScrW Scr4/+ males (mutations in cis).

      (Bantignies et al., 2011)

      Df(2R)ED3921; Scr4/+ double mutants show a greater reduction in sex comb teeth than Scr4/+ single mutants.

      (Ragab et al., 2006)

      Scr4 Antp25 embryos show a wild-type oenocyte pattern.

      (Brodu et al., 2002)

      Scr4/Df(3R)Antp7 animals show 11% viability. Scr4/Df(3R)Antp7 males have an average of 5.6 sex comb teeth on the first leg.

      (Southworth and Kennison, 2002)

      pb1 Scr5/pb5 Scr4 flies show transformation of the proboscis into the antenna. The transformation varies from thick and shortened aristae with claws and pulvillae to apparently complete antennae with aristae, third and second antennal segments bearing characteristic bristles.

      (Abzhanov et al., 2001)

      Dsp11/Y ; Scr4/+ males show an increase in the severity of the phenotype; the size of the sex comb teeth is greatly reduced, with an average number of 4 teeth in the double mutant versus either single mutant combination.

      (Decoville et al., 2001)

      The addition of Scr4 to pbhs.PB, skdpap-53 flies suppresses the distal sex comb phenotype seen in these animals. MED17s2956, ScrP, skdpap-53 flies exhibit a stronger ectopic distal sex comb phenotype than any of the double mutant combinations.

      (Boube et al., 2000)

      scd1/Y Scr4/+ males lack the small distal sex comb found in scd1/Y males, but the sex comb on the first tarsal segment of the leg contains more teeth than the sex comb of Scr4/+ heterozygous males.

      (Boube et al., 1997)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (5)
      Notes on Origin
      Discoverer

      Wakimoto.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      References (57)