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General Information
Symbol
Dmel\sgg32
Species
D. melanogaster
Name
FlyBase ID
FBal0015510
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sggD127, zw3D127, zw3sggD127
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

29.2% of eggs derived from mothers carrying sgg32 mutant germline clones are able to complete meiosis. The remaining 70.8% exhibit abnormal meiotic defects.

sgg32 mitotic clones result in extra macrochaetae and microchaetae in the dorsal part and scutellum of the adult notum, which corresponds to the wg-expressing region.

Homozygous mutant clones give rise to ectopic bristles on the adult wing.

Homozygous clones in the anterior of the wing form small outgrowths.

sgg32 somatic clones in tergites are abnormally round in shape and have higher than normal bristle densities. In addition polarity reversals are seen; hairs and bristles at the back of clones are reversed. sgg32 somatic clones in the adult abdomen can transform segmental region anterior 1 (a1) cuticle into segmental region anterior 3 (a3) cuticle. Such clones in the segmental region posterior 2 (p2) can sometimes become hairy, suggesting a transformation to region p3.

Large homozygous clones that cover most of the dorsal wing hinge cause duplication of axillary sclerite 3 at the expense of axillary sclerite 1, axillary sclerite 2 and the unnamed plate. Clones in the ventral wing hinge cause reduction or loss of the axillary pouch, depending on the size of the clone.

Homozygous sgg32 or sgg32/sggM11 adults (that have been rescued to adulthood by the addition of sgghs.P coupled with larval and pupal heatshocks), show a lengthening of the locomotor activity rhythm periodicity to about 26 hours.

Homozygous clones autonomously form extra macrochaetae in the notum. These appear most frequently near the dorsocentral meridian and in the scutellum and less frequently near the lateral macrochaetae. Extra macrochaetae are also seen medially in the scutum and prescutum, far from the normal site of any wild-type macrochaetae. The macrochaetae are confined to the posterior region of the clone, irrespective of the location of the clone in the notum.

sgg32 Df(1)sc-B57 clones in the wing show no disturbance in planar polarity.

Homozygous clones in the eye cause replacement of ommatidia by ectopic cuticle, which shows the typical ridged morphology of frons cuticle.

sgg32 mutant cells within clones present in the eye fail to differentiate and progression of the morphogenetic furrow is blocked. Wild type cells anterior to the clone fail to express neuronal markers confirming that the morphogenetic furrow is unable to reinitiate beyond a block caused by the wg pathway.

Clones of mutant cells induced in the leg show that removal of sgg activity is sufficient to specify ventral cell fate. Such clones can reorganise the dorsal-ventral axis of the leg. Ventrally located sgg clones show a local increase in bristle density due to a failure in lateral inhibition of neuronal fate specification. Bifurcations produced by clones in the tibia or femur are unable to extend beyond the end of the dsegment in which the bifurcation occurred. Dorsally located clones lead to the formation of duplicated legs by respecifying the fates of surrounding cells. Clones in lateral positions cause simple outgrowths of leg that consist entirely of mutant cells. The mutant cells do not affect the fate of the wild type cells around them. Mutant clones in the lateral region of the leg do not respecify cell fate. Pattern respecification occurs only when the clones are located dorsally, near the endogenous anterior-posterior compartment boundary. Duplicated legs always bifurcate from the dorsal side of the endogenous leg.

Epistatic to NAx-1, whereas Nl1N-ts1 is epistatic to sgg32.

Homozygous clones in the thorax differentiate a cluster of bristles at the site of a single bristle in wild-type flies. In 98% of cases that respond, individual bristles of the same mutant cluster behave similarly to one another and elicit a cleaning response from the same leg. The mutant bristles elicit the same behavioural response as wild-type bristles when stimulated.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

sgg32 has visible | recessive | somatic clone phenotype, enhanceable by Su(fu)LP

NOT Enhancer of
Statement
Reference

sgg[+]/sgg32 is a non-enhancer of locomotor rhythm defective phenotype of timblind

Suppressor of
Statement
Reference

sgg32 is a suppressor of visible | recessive | somatic clone phenotype of NMcd1

NOT Suppressor of
Statement
Reference

sgg[+]/sgg32 is a non-suppressor of locomotor rhythm defective phenotype of timblind

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

sgg32 has wing | anterior | somatic clone phenotype, enhanceable by Su(fu)LP

NOT Enhanced by
Statement
Reference

sgg32 has wing phenotype, non-enhanceable by Atg4bP0997/Atg4bP0997

Suppressed by
Statement
Reference

sgg32 has macrochaeta & wing | ectopic | somatic clone phenotype, suppressible by pan2

NOT suppressed by
Statement
Reference

sgg32 has wing phenotype, non-suppressible by Atg4bP0997/Atg4bP0997

sgg32 has phenotype, non-suppressible by Rnor\Gsk3ahs.PR

Enhancer of
Suppressor of
Statement
Reference

sgg32 is a suppressor of adult thorax & microchaeta phenotype of NMcd1

Other
Additional Comments
Genetic Interactions
Statement
Reference

The sgg32 mutation leads to an enhancement in the number of flies that show extra macrochaetae on the scutellum when NcC318G.Scer\UAS is expressed under the control of Scer\GAL4sca-P309.

The increase in locomotor rhythm period length seen in timblind homozygous flies in free-running conditions is not further increased if the flies also carry sgg32.

Somatic clones homozygous for both sgg32 and pan2 show a suppression of the ectopic bristle phenotype seen in sgg32 clones alone.

sgg32 Su(fu)LP double mutant clones in the anterior of the wing organise the formation of a duplicated wing. The double mutant cells form ectopic sensory bristles. Most of the duplicated wing is derived from wild-type cells.

Unlike Pka-C1E95 clones, sgg32 Pka-C1E95 double mutant clones in the medial prescutum produce macrochaetae autonomously, and unlike sgg32 clones, the double mutant clones have a symmetrical bristle pattern. Formation of macrochaetae within the double mutant clones is increased relative to sgg32 clones. In the posterior scutum, sgg32 Pka-C1E95 double mutant clones have a scutellar phenotype very similar to that of Pka-C1E95 clones. In the anterior scutum, sgg32 Pka-C1E95 double mutant clones no longer show the non-autonomous induction of macrochaetae or formation of pits characteristic of Pka-C1E95 clones. Formation of macrochaetae within the double mutant clones is increased relative to sgg32 clones.

No significant effect on the scaMSKF mutant phenotype.

cnomis1 homozygotes that are heterozygous with sgg32 exhibit extra wing vein material.

Xenogenetic Interactions
Statement
Reference

Rnor\Gsk-3αhs.PR and Rnor\Gsk-3βhs.PR do not rescue sgg32 at all, even under maximal heat shock. Rnor\Gsk-3βhs.PR can rescue sgg32 in wing disc clones, therefore can replace sgg during the epidermal-neural cell fate decision, but not at other stages of life cycle that sgg is required.

Complementation and Rescue Data
Rescued by
Partially rescued by

sgg32 is partially rescued by sggmR39

sgg32 is partially rescued by sggmR10

Not rescued by

sgg32 is not rescued by sggmR46

Comments

Expression of sggScer\UAS.P\T.cTa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the meiotic defects seen in sgg32 mutant germline clones.

sggmR10 rescues hemizygous males to pharate adults. sggmR39 rescues similarly but less efficiently, but sggmR46 does not rescue at all.

Maximal heat shock of mutant alleles bearing sgghs.P rescues the sgg32 mutant phenotype. Lower heat shock produces bristle hyperplasia expected of a hypomorphic allele, a phenotype similar to that of Nl1N-ts1 when exposed to the restrictive temperature during early pupal periods.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Ripoll.

Comments
Comments

None of the multiple products of the sgg locus remain in this allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
References (48)