Allele Dmel\Sh²⁵

General Information
Symbol	Dmel\Sh²⁵	Species	D. melanogaster
Name		FlyBase ID	FBal0015565
Feature type	allele	Associated gene	Dmel\Sh
Also Known As	W32

Allele class
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	X ray (Kamb et al., 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Breakpoint within the Sh coding region, 3' to the exon encoding the "D" 5' alternative end. This prevents transcription of all transcripts containing the "B" and "D" 5' alternative ends. (Hardie et al., 1991) Translocation breakpoint is at map position +95.2 to +98.7. (Kamb et al., 1987) break at 95.2 to 98.7; relative to the Sh chromosomal walk in kb (Kamb, Iverson and Tanouye, 1987)
Caused by aberration	T(1;Y)W32 (Kamb et al., 1987, Ferrus et al., 1990, Balakrishnan and Rodrigues, 1991, Hardie et al., 1991, Hevers and Hardie, 1995, de la Pompa, 1994, Rogero et al., 1997, Wu et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective \| dominant (Ferrus et al., 1990) neurophysiology defective (Ferrus et al., 1990, ) neurophysiology defective (with Sh⁸) (Wu et al., 2010) taste perception defective (Balakrishnan and Rodrigues, 1991)
Phenotype Manifest In

Detailed Description
	Statement Reference The spontaneous miniature excitatory junctional potential (mEJP) frequency is significantly increased in Sh[8]/Sh[25] mutant larvae compared with wild-type controls. (Wu et al., 2010) Application of serotonin failed to alter modulation of the potassium channel in a semi-intact preparation of the retina. (Hevers and Hardie, 1995) Flies show a typical Sh mutant leg shaking phenotype. Hemizygous males show a reduced preference for sucrose compared to wild-type in feeding preference tests, probably due to a shift in the threshold of detection. The response of flies to 100mM NaCl and 0.2M KCl is not significantly different to wild-type. Flies show an increased tolerance to 0.5M NaCl compared to wild-type, due to an increase in the threshold of repulsion. (Balakrishnan and Rodrigues, 1991) Photoreceptor A channels have abnormal inactivation kinetics; the rate of inactivation is faster than wild-type. (Hardie et al., 1991) Shaking phenotype: K+ muscle current is abnormal and the action potential from the CGF interneuron is defective. (Ferrus et al., 1990) abnormal leg shaking under ether anesthesia; abnormal A-type potassium currents in larval muscle and/or pupal flight muscle; abnormal action potentials in the adult cervical giant fiber
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	Merriam. Kennison.

Comments
	Excludes all ShB and ShD transcripts at the 5' end of Sh. (Hevers and Hardie, 1995) The severity of the sucrose preference defect shows the following order: Sh⁸ > Sh⁵ = Sh⁷ > Sh²⁵ > T(1;Y)V7 > Sh¹⁴. The degree of tolerance to 0.5M NaCl shows the order: Sh⁸ > Sh⁷ = Sh⁵ > T(1;Y)V7 = Sh²⁵ > Sh¹⁴. (Balakrishnan and Rodrigues, 1991)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Sh²⁵ Sh^W32 (Ferrus et al., 1990, ) unnamed (Rogero et al., 1997, Hardie et al., 1991) W32 (Balakrishnan and Rodrigues, 1991, Wu et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Jepson et al., 2012, PLoS Genet. 8(4): e1002671 dyschronic, a Drosophila Homolog of a Deaf-Blindness Gene, Regulates Circadian Output and Slowpoke Channels. [FBrf0218124] Wu et al., 2010, Nat. Neurosci. 13(1): 69--75 SLEEPLESS, a Ly-6/neurotoxin family member, regulates the levels, localization and activity of Shaker. [FBrf0209634] Rogero et al., 1997, J. Neurosci. 17(13): 5108--5118 Diverse expression and distribution of Shaker potassium channels during the development of the Drosophila nervous system. [FBrf0093676] Hevers and Hardie, 1995, Neuron 14(4): 845--856 Serotonin modulates the voltage dependence of delayed rectifier and Shaker potassium channels in Drosophila photoreceptors. [FBrf0080090] de la Pompa, 1994, Mol. Gen. Genet. 244(2): 197--204 Functional relationships between genes of the Shaker gene complex of Drosophila. [FBrf0080564] Balakrishnan and Rodrigues, 1991, J. Exp. Biol. 157: 161--181 The Shaker and shaking-B genes specify elements in the processing of gustatory information in Drosophila melanogaster. [FBrf0054538] Hardie et al., 1991, Neuron 6(3): 477--486 Novel potassium channels encoded by the Shaker locus in Drosophila photoreceptors. [FBrf0054810] Ferrus et al., 1990, Genetics 125: 383--398 Genetic analysis of the Shaker gene complex of Drosophila melanogaster. [FBrf0051942] Baumann et al., 1987, EMBO J. 6: 3419--3429 Molecular organization of the maternal effect region of the Shaker complex of Drosophila: characterization of an IA Channel transcript with homology to vertebrate Na⁺ channel. [FBrf0046121] Kamb et al., 1987, Cell 50: 405--413 Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel. [FBrf0045758] Papazian et al., 1987, Science 237: 749--753 Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila. [FBrf0047308] Salkoff, 1983, Cold Spring Harbor Symp. Quant. Biol. 48(1): 221--231 Genetic and voltage-clamp analysis of a Drosophila potassium channel. [FBrf0039047] Tanouye et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78(10): 6548--6552 Abnormal action potentials associated with the Shaker complex locus of Drosophila. [FBrf0037273]

Allele Dmel\Sh25

Allele Dmel\Sh²⁵