FlyBase Allele Report: Dmel\shakB[11]

General Information

Symbol

Dmel\shakB¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0015584

Feature type

allele

Associated gene

Dmel\shakB

Associated Insertion(s)

Carried in Construct

Also Known As

R-9-29

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutation in the lethal and neural common exons. Amino acid replacement: W262@. Nucleotide substitution: TGG to TGA. Residue lies in the membrane-spanning domain.

(Krishnan et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:20,773,259..20,773,259 [-]

Nucleotide change:

G20773259A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

W262term

Comment:

TGG to TGA

(Krishnan et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Baird et al., 1990, Miklos et al., 1987)

lethal

(Balakrishnan and Rodrigues, 1991)

lethal | recessive

(Krishnan et al., 1995, Kramers et al., 1983)

uncoordinated

(Baird et al., 1990)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell

(Krishnan et al., 1995)

mesothoracic tergotrochanter muscle cell

(Krishnan et al., 1995)

muscle cell of abdominal 2 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 2 ventral oblique muscle 5

(Todman et al., 1999)

muscle cell of abdominal 3 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 3 ventral oblique muscle 5

(Todman et al., 1999)

muscle cell of abdominal 4 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 4 ventral oblique muscle 5

(Todman et al., 1999)

muscle cell of abdominal 5 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 5 ventral oblique muscle 5

(Todman et al., 1999)

muscle cell of abdominal 6 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 6 ventral oblique muscle 5

(Todman et al., 1999)

muscle cell of abdominal 7 ventral oblique muscle 4

(Todman et al., 1999)

muscle cell of abdominal 7 ventral oblique muscle 5

(Todman et al., 1999)

Detailed Description

Statement

Reference

Carboxyfluorescein injected into abdominal muscle VL3 fails to spread into muscles VO4 and VO5, in contrast to wild type, indicating a loss of gap-junctional connections between these muscles. Muscles VO4 and VO5 have significantly smaller I_A and I_K currents than wild type.

(Todman et al., 1999)

The DLMs do not respond to brain shocks and the TTMs respond only occasionally and then with a longer than normal latency.

(Krishnan et al., 1995)

Reduction in anterior-posterior extent of the medial branch of the tergotrochantral muscle motorneuron. Posterior branch, and position of the motorneuron cell body are unaffected.

(Baird et al., 1993)

shakB¹¹ fails to complement the sucrose gustatory defect of shakB¹ or shakB³.

(Balakrishnan and Rodrigues, 1991)

Abnormal electrophysiological phenotype. Homozygous flies exhibit uncoordinated leg movements under ether anaesthesia and do not jump to a light off stimulus. Heterozygotes are fully viable but exhibit some neuronal defects.

(Baird et al., 1990)

shakB¹¹/shakB² flies show shaking behaviour.

(Miklos et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

shakB¹⁵

(Munoz and Cook, 2004.4.7)

Comments

Fails to complement shakB³.

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lifschytz.

Comments

shakB alleles exhibit a complex pattern of complementation.

(Baird et al., 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Pas^R-9-29

(Baird et al., 1990)

R-9-29

(Krishnan et al., 1995, Balakrishnan and Rodrigues, 1991, Miklos et al., 1987, Zusman et al., 1985, Kramers et al., 1983, Lefevre, 1981, Lifschytz and Falk, 1969)

l(1)R-9-29

(Munoz and Cook, 2004.4.7, Miklos et al., 1986)

shak-B^R-9-29

(Todman et al., 1999)

shakB¹¹

shakB^R-9-29

(Perrimon et al., 1989)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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