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General Information
Symbol
Dmel\shakB11
Species
D. melanogaster
Name
FlyBase ID
FBal0015584
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R-9-29
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G20773259A

Reported nucleotide change:

G?A

Amino acid change:

W273term | shakB-PA; W262term | shakB-PC; W433term | shakB-PD; W217term | shakB-PE; W193term | shakB-PF; W278term | shakB-PG; W278term | shakB-PH; W278term | shakB-PI

Reported amino acid change:

W262term

Comment:

TGG to TGA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation in the lethal and neural common exons. Amino acid replacement: W262@. Nucleotide substitution: TGG to TGA. Residue lies in the membrane-spanning domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Carboxyfluorescein injected into abdominal muscle VL3 fails to spread into muscles VO4 and VO5, in contrast to wild type, indicating a loss of gap-junctional connections between these muscles. Muscles VO4 and VO5 have significantly smaller IA and IK currents than wild type.

The DLMs do not respond to brain shocks and the TTMs respond only occasionally and then with a longer than normal latency.

Reduction in anterior-posterior extent of the medial branch of the tergotrochantral muscle motorneuron. Posterior branch, and position of the motorneuron cell body are unaffected.

shakB11 fails to complement the sucrose gustatory defect of shakB1 or shakB3.

Abnormal electrophysiological phenotype. Homozygous flies exhibit uncoordinated leg movements under ether anaesthesia and do not jump to a light off stimulus. Heterozygotes are fully viable but exhibit some neuronal defects.

shakB11/shakB2 flies show shaking behaviour.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments

Fails to complement shakB3.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Lifschytz.

Comments
Comments

shakB alleles exhibit a complex pattern of complementation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (14)