The absence of enteroendocrine cells in adult midgut sina2 clones is suppressed by the clonal expression of ttkGD4414 under the control of Scer\GAL4Act.PU, even leading to their severe increase in number as compared to control clones.
In sina2,msi1/sina3,msi1 mutant third instar larval eye discs significantly reduced numbers of R1 and R6 photoreceptor cells are observed. Very few R7 cells are recruited to the developing ommatidia in these mutants, decreased in number compared to sina2/sina3 mutant animals.
Expression of ttkGD4414 under the control of Scer\GAL4lz-gal4 in sina2,msi1/sina3,msi1 mutant third instar larval eye discs, significantly rescues the number of R1 and R6 cells, although recruitment of these cells appears delayed. Ectopic R7 cells are observed in most developing ommatidia.
Double mutants of sina2/sina3 with msi1 show a synergistic, not additive, eye phenotype. Ommatidial arrays are disturbed, eyes consequently roughened and ommatidia do not contain more than five rhabdomeres. Cone cell array is disturbed. 30% of the ommatidia of sina2 msi1 ttkosn/sina3 msi1 show the normal number and arrangement of photoreceptor cells. The cone cell defects of sina2 msi1/sina3 msi1 are also suppressed by ttkosn.
The phylGMR.PC eye phenotype is completely suppressed in a sina2/sina3 background; the eyes are normal, except for the lack of R7 photoreceptor cells due to the absence of sina function. sina2/sina3 flies carrying both phylGMR.PC and ttk88.GMR.T:Hsap\MYC have very disrupted eyes, similar to those of flies carrying ttk88.GMR.T:Hsap\MYC alone.