Allele Dmel\sina³

General Information
Symbol	Dmel\sina³	Species	D. melanogaster
Name		FlyBase ID	FBal0015667
Feature type	allele	Associated gene	Dmel\sina

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Lindsley and Zimm, 1992)
Mutagen	ethyl methanesulfonate (Lindsley and Zimm, 1992)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion 3L:16,850,150..16,850,457 comment=Deletion of 308 bp; coordinates determined from Figure 5B of FBrf0051381 and differ from the coordinates listed in Table 1. evidence=experimental (Carthew and Rubin, 1990)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Deletion of 308bp of sina coding region, from 1534 through to 1842bp. This brings valine 211 in frame with the sina open reading frame stop codon. The resulting sina³ protein is truncated, missing the 103 most C-terminal amino acids. (Carthew and Rubin, 1990)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective \| adult stage (Carthew and Rubin, 1990) female sterile (Carthew and Rubin, 1990) male sterile (Carthew and Rubin, 1990) uncoordinated (Carthew and Rubin, 1990) viable (with sina²) (Pi et al., 2001) viable \| recessive (Carthew and Rubin, 1990) visible (with sina²) (Pi et al., 2001, Okabe et al., 2001) visible \| adult stage (Carthew and Rubin, 1990)
Phenotype Manifest In
	adult abdominal segment 3 & chaeta (with sina²) (Pi et al., 2001) adult abdominal segment 3 & microchaeta (with sina²) (Pi et al., 2001) adult abdominal segment 3 & tormogen cell \| supernumerary (with sina²) (Pi et al., 2001) adult abdominal segment 3 & trichogen cell \| supernumerary (with sina²) (Pi et al., 2001) adult abdominal segment 4 & chaeta (with sina²) (Pi et al., 2001) adult abdominal segment 4 & microchaeta (with sina²) (Pi et al., 2001) adult abdominal segment 4 & tormogen cell \| supernumerary (with sina²) (Pi et al., 2001) adult abdominal segment 4 & trichogen cell \| supernumerary (with sina²) (Pi et al., 2001) dorsal double row (Carthew and Rubin, 1990) external sensory organ (with sina²) (Okabe et al., 2001) eye (with sina²) (Hirota et al., 1999) eye photoreceptor cell (Carthew and Rubin, 1990) macrochaeta (Carthew and Rubin, 1990) medial triple row (Carthew and Rubin, 1990) mesothoracic tergum & chaeta (with sina²) (Pi et al., 2001) mesothoracic tergum & microchaeta (with sina²) (Pi et al., 2001) mesothoracic tergum & tormogen cell \| supernumerary (with sina²) (Pi et al., 2001) mesothoracic tergum & trichogen cell \| supernumerary (with sina²) (Pi et al., 2001) microchaeta (Carthew and Rubin, 1990) ommatidium (with sina²) (Hirota et al., 1999, Bai et al., 2001, Siddall et al., 2009) photoreceptor cell R1 \| third instar larval stage (with sina²) (Siddall et al., 2009) photoreceptor cell R6 \| third instar larval stage (with sina²) (Siddall et al., 2009) photoreceptor cell R7 (Tang et al., 1997, Yamamoto et al., 1996, Carthew and Rubin, 1990) photoreceptor cell R7 (with sina²) (Hirota et al., 1999, Bai et al., 2001) photoreceptor cell R7 \| third instar larval stage (with sina²) (Siddall et al., 2009) photoreceptor cell R8 of pale ommatidium (with sina²) (Birkholz et al., 2009) trichogen cell \| supernumerary (with sina²) (Okabe et al., 2001)
Detailed Description
	Statement Reference The number of pale-type R8 cells is dramatically reduced in the eyes of sina[2]/sina[3] animals. (Birkholz et al., 2009) sina²/sina³ lack R7 photoreceptors. (Bai et al., 2001) Mutants show a double- bristle phenotype. (Okabe et al., 2001) 41% of the notal microchaetae and 62% of the microchaetae on abdominal segments 3 and 4 are missing in sina²/sina³ flies. 17.2% of bristles on the notum are duplicated, having 2 hairs emerging from 2 sockets or a fused socket. 44.5% of bristles on abdominal segments 3 and 4 are duplicated (2 hairs/2 sockets) and 20.9% show other defects (including 1 hair surrounded by 2-3 sockets, and 2 socket or 4 socket clusters without shafts). Embryonic development, including development of the es organs, is normal in sina²/sina³ animals. (Pi et al., 2001) sina²/sina³ mutants show missing R7 cells in 90% of the ommatidia. The external morphology of the eye shows a slight roughness. (Hirota et al., 1999) Homozygotes lack R7 photoreceptor cells. This phenotype is unaltered if the flies also carry aop^pok-1. R7-like cells are produced in sina³ Df(3R)E40/sina³ + flies. (Yamamoto et al., 1996) Amorph based on the phenotypes of hemizygous <up>sina¹/Df(3L)st-g18</up> versus that of homozygous flies. (Lindsley and Zimm, 1992) This allele probably represents complete loss of function of the sina gene. In the ommatidia the R7 photoreceptor cell is always missing, the R7 precursor adopting a cone cell fate, and sometimes one or two cells of the R1-R6 class are missing as well. The normal trapezoidal arrangement of the R1-R6 cells is disorganised, and the R8 cell is often found in uncharacteristic positions. Other adult sensory organs are also affected: sensory bristles distributed over the body surface are frequently missing, with neither the cuticular bristle sheath nor the socket being present, affected sensory organs including the microchaetae and macrochaetae of the head, notum, abdomen and wing margin, and the stout and slender bristles of the anterior wing margin. The phenotype is most severe with bristles located on the abdomen, where often only a minor fraction of the normal number of bristles are present. Also, many of the remaining bristles have two or three sheaths emerging from a single socket. There is also a 10-fold reduction in adult lifespan, and adult behaviour is generally lethargic and uncoordinated. Neither males nor females are fertile, although they produce morphologically normal sperm and eggs, respectively. (Carthew and Rubin, 1990)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference sina³/sina² has visible phenotype, enhanceable by msi¹/msi¹ (Okabe et al., 2001) sina³/sina² has visible phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has visible phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001)
Suppressed by
Statement Reference sina³/sina² has visible phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001)
Enhancer of
Statement Reference sina³/sina² is an enhancer of visible \| recessive phenotype of msi¹ (Okabe et al., 2001) sina³/sina² is an enhancer of visible phenotype of msi¹ (Hirota et al., 1999)
NOT Enhancer of
Statement Reference sina³ is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) sina³ is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002)
Suppressor of
Statement Reference sina³ is a suppressor of visible phenotype of Src64B^{Δ540.hs.2sev} (Kussick et al., 1993)
NOT Suppressor of
Statement Reference sina³ is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) sina³ is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002)
Phenotype Manifest In
Enhanced by
Statement Reference sina³/sina² has adult abdominal segment 3 & chaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & chaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has external sensory organ phenotype, enhanceable by msi¹/msi¹ (Okabe et al., 2001) sina³/sina² has mesothoracic tergum & chaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has mesothoracic tergum & chaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has mesothoracic tergum & tormogen cell \| supernumerary phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has mesothoracic tergum & tormogen cell \| supernumerary phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has mesothoracic tergum & trichogen cell \| supernumerary phenotype, enhanceable by phyl[+]/phyl²²⁴⁵ (Pi et al., 2001) sina³/sina² has mesothoracic tergum & trichogen cell \| supernumerary phenotype, enhanceable by phyl²/phyl[+] (Pi et al., 2001) sina³/sina² has photoreceptor cell R1 \| third instar larval stage phenotype, enhanceable by msi¹/msi¹ (Siddall et al., 2009) sina³/sina² has photoreceptor cell R6 \| third instar larval stage phenotype, enhanceable by msi¹/msi¹ (Siddall et al., 2009) sina³/sina² has photoreceptor cell R7 \| third instar larval stage phenotype, enhanceable by msi¹/msi¹ (Siddall et al., 2009)
Suppressed by
Statement Reference msi¹, sina³/sina² has cone cell phenotype, suppressible by ttk^osn (Hirota et al., 1999) msi¹, sina³/sina² has ommatidium phenotype, suppressible by ttk^osn (Hirota et al., 1999) msi¹, sina³/sina² has photoreceptor cell R1 \| third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4 (Siddall et al., 2009) msi¹, sina³/sina² has photoreceptor cell R6 \| third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4 (Siddall et al., 2009) msi¹, sina³/sina² has photoreceptor cell R7 \| third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4 (Siddall et al., 2009) msi¹, sina³/sina² has rhabdomere phenotype, suppressible by ttk^osn (Hirota et al., 1999) sina³/sina² has adult abdominal segment 3 & chaeta phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 3 & tormogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 3 & trichogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & chaeta phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & tormogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has adult abdominal segment 4 & trichogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has mesothoracic tergum & chaeta phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has mesothoracic tergum & tormogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has mesothoracic tergum & trichogen cell \| supernumerary phenotype, suppressible by ttk[+]/ttk^osn (Pi et al., 2001) sina³/sina² has ommatidium phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001) sina³/sina² has photoreceptor cell R7 phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001) sina³ has phenotype, suppressible \| partially by ttk^osn (Hirota et al., 1999)
Enhancer of
Statement Reference sina³/sina² is an enhancer of external sensory organ phenotype of msi¹ (Okabe et al., 2001) sina³/sina² is an enhancer of ommatidium phenotype of msi¹ (Hirota et al., 1999)
NOT Enhancer of
Statement Reference sina³ is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) sina³ is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002)
Suppressor of
Statement Reference sina³/sina² is a suppressor of eye phenotype of phyl^GMR.PC (Tang et al., 1997) sina³ is a suppressor of eye phenotype of Src64B^{Δ540.hs.2sev} (Kussick et al., 1993) sina³ is a suppressor of phenotype of phyl^hs.sev (Tang et al., 1997)
NOT Suppressor of
Statement Reference sina³ is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) sina³ is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002)
Other
Statement Reference msi¹, sina³/sina², ttk^osn has cone cell phenotype (Hirota et al., 1999) msi¹, sina³/sina², ttk^osn has ommatidium phenotype (Hirota et al., 1999) msi¹, sina³/sina², ttk^osn has rhabdomere phenotype (Hirota et al., 1999) sina¹/sina³, svp^1.sev has photoreceptor cell R7 phenotype (Hiromi et al., 1993) sina¹/sina³, svp^2.sev has photoreceptor cell R7 phenotype (Hiromi et al., 1993, Hiromi et al., 1993)
Additional Comments
Genetic Interactions
Statement Reference Double mutants of sina²/sina³ with msi¹ show a synergistic, not additive, eye phenotype. Ommatidial arrays are disturbed, eyes consequently roughened and ommatidia do not contain more than five rhabdomeres. Cone cell array is disturbed. 30% of the ommatidia of sina² msi¹ ttk^osn/sina³ msi¹ show the normal number and arrangement of photoreceptor cells. The cone cell defects of sina² msi¹/sina³ msi¹ are also suppressed by ttk^osn. (Hirota et al., 1999) The phyl^GMR.PC eye phenotype is completely suppressed in a sina²/sina³ background; the eyes are normal, except for the lack of R7 photoreceptor cells due to the absence of sina function. sina²/sina³ flies carrying both phyl^GMR.PC and ttk^{88.GMR.T:Hsap\MYC} have very disrupted eyes, similar to those of flies carrying ttk^{88.GMR.T:Hsap\MYC} alone. (Tang et al., 1997) sina¹/sina³; svp^1.sev and sina¹/sina³; svp^2.sev flies differentiate neither normal nor ectopic R7 cells. (Hiromi et al., 1993)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	5780 st¹ sina³/TM6B, Tb¹ 26270 w^*; sina³ P{neoFRT}80B/TM3, Sb¹
Kyoto	108422 st¹ sina³/TM6B, Tb¹
Notes on Origin
Discoverer

Comments
	No interaction with P{sev-svp1} or P{sev-svp2} exists. (Begemann et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	sina³ (Birkholz et al., 2009, Siddall et al., 2009) Sina³ (Cooper, 2007) sina^A16 (Lindsley and Zimm, 1992)
Name Synonym
Secondary FlyBase IDs
	FBal0056160
References ( 23 )

Generate a list of	All references relating to this allele ( 23 )

List References by type	Research paper ( 21 ) Book ( 1 ) Letter ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...

Allele Dmel\sina3

Allele Dmel\sina³