FlyBase Allele Report: Dmel\sina[3]

General Information

Symbol

Dmel\sina³

Species

D. melanogaster

Name

FlyBase ID

FBal0015667

Feature type

allele

Associated gene

Dmel\sina

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Lindsley and Zimm, 1992)

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992)

Progenitor genotype

Cytology

Description

Deletion of 308bp of sina coding region, from 1534 through to 1842bp. This brings valine 211 in frame with the sina open reading frame stop codon. The resulting sina³ protein is truncated, missing the 103 most C-terminal amino acids.

(Carthew and Rubin, 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:16,857,050..16,857,357 [+]

Comment:

Deletion of 308 bp; coordinates determined from Figure 5B of FBrf0051381 and differ from the coordinates listed in Table 1.

(Carthew and Rubin, 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage

(Carthew and Rubin, 1990)

abnormal meiotic cell cycle | female (with sina^A4)

(Hughes et al., 2019)

decreased cell number | adult stage | somatic clone

(Yin and Xi, 2018)

female semi-fertile (with sina^A4)

(Hughes et al., 2019)

female sterile

(Carthew and Rubin, 1990)

male sterile

(Carthew and Rubin, 1990)

short lived

(Carthew and Rubin, 1990)

uncoordinated

(Carthew and Rubin, 1990)

viable (with sina²)

(Pi et al., 2001)

viable | recessive

(Carthew and Rubin, 1990)

visible (with sina²)

(Okabe et al., 2001, Pi et al., 2001)

visible | adult stage

(Carthew and Rubin, 1990)

Phenotype Manifest In

adult abdominal segment 3 & chaeta (with sina²)

(Pi et al., 2001)

adult abdominal segment 3 & microchaeta (with sina²)

(Pi et al., 2001)

adult abdominal segment 3 & tormogen cell | supernumerary (with sina²)

(Pi et al., 2001)

adult abdominal segment 3 & trichogen cell | supernumerary (with sina²)

(Pi et al., 2001)

adult abdominal segment 4 & chaeta (with sina²)

(Pi et al., 2001)

adult abdominal segment 4 & microchaeta (with sina²)

(Pi et al., 2001)

adult abdominal segment 4 & tormogen cell | supernumerary (with sina²)

(Pi et al., 2001)

adult abdominal segment 4 & trichogen cell | supernumerary (with sina²)

(Pi et al., 2001)

adult midgut | somatic clone

(Yin and Xi, 2018)

dorsal row of double row of wing sensilla

(Carthew and Rubin, 1990)

enteroendocrine cell | adult stage | somatic clone

(Yin and Xi, 2018)

external sensory organ (with sina²)

(Okabe et al., 2001)

eye (with sina²)

(Hirota et al., 1999)

eye photoreceptor cell

(Carthew and Rubin, 1990)

female germline cyst | oogenesis (with sina^A4)

(Hughes et al., 2019)

female germline cyst | oogenesis stage S1, with Df(3L)sina^SH

(Hughes et al., 2019)

macrochaeta

(Carthew and Rubin, 1990)

medial row of triple row of wing sensilla

(Carthew and Rubin, 1990)

mesothoracic tergum & chaeta (with sina²)

(Pi et al., 2001)

mesothoracic tergum & microchaeta (with sina²)

(Pi et al., 2001)

mesothoracic tergum & tormogen cell | supernumerary (with sina²)

(Pi et al., 2001)

mesothoracic tergum & trichogen cell | supernumerary (with sina²)

(Pi et al., 2001)

microchaeta

(Carthew and Rubin, 1990)

ommatidium (with sina²)

(Siddall et al., 2009, Bai et al., 2001, Hirota et al., 1999)

photoreceptor cell R1 | third instar larval stage (with sina²)

(Siddall et al., 2009)

photoreceptor cell R6 | third instar larval stage (with sina²)

(Siddall et al., 2009)

photoreceptor cell R7

(Tang et al., 1997, Yamamoto et al., 1996, Carthew and Rubin, 1990)

photoreceptor cell R7 (with sina²)

(Bai et al., 2001, Hirota et al., 1999)

photoreceptor cell R7 | third instar larval stage (with sina²)

(Siddall et al., 2009)

photoreceptor cell R8 of pale ommatidium (with sina²)

(Birkholz et al., 2009)

synaptonemal complex | oogenesis (with sina^A4)

(Hughes et al., 2019)

synaptonemal complex | oogenesis stage S1, with Df(3L)sina^SH

(Hughes et al., 2019)

trichogen cell | increased number (with sina²)

(Okabe et al., 2001)

Detailed Description

Statement

Reference

sina³/sina^A4 and sina³/Df(3L)sina^SH transheterozygotes exhibit frequent X-chromosome and 4[[th]]-chromosome non-disjunction, as well as defects in synaptonemal complex assembly during oogenesis, with components forming abnormal polycomplexes, which are observed in both oocytes and nurse cells by stage 2.

sina³/Df(3L)sina^SH transheterozygotes exhibit defects in synaptonemal complex assembly during oogenesis, with components forming abnormal polycomplexes

(Hughes et al., 2019)

sina³ homozygous clones in the adult midgut virtually lack enteroendocrine cells, unlike the expected 6-8% in control clones, but exhibit a similar number of cells as compared to controls.

(Yin and Xi, 2018)

The number of pale-type R8 cells is dramatically reduced in the eyes of sina²/sina³ animals.

(Birkholz et al., 2009)

sina²/sina³ lack R7 photoreceptors.

(Bai et al., 2001)

Mutants show a double- bristle phenotype.

(Okabe et al., 2001)

41% of the notal microchaetae and 62% of the microchaetae on abdominal segments 3 and 4 are missing in sina²/sina³ flies. 17.2% of bristles on the notum are duplicated, having 2 hairs emerging from 2 sockets or a fused socket. 44.5% of bristles on abdominal segments 3 and 4 are duplicated (2 hairs/2 sockets) and 20.9% show other defects (including 1 hair surrounded by 2-3 sockets, and 2 socket or 4 socket clusters without shafts). Embryonic development, including development of the es organs, is normal in sina²/sina³ animals.

(Pi et al., 2001)

sina²/sina³ mutants show missing R7 cells in 90% of the ommatidia. The external morphology of the eye shows a slight roughness.

(Hirota et al., 1999)

Homozygotes lack R7 photoreceptor cells. This phenotype is unaltered if the flies also carry aop^pok-1. R7-like cells are produced in sina³ Df(3R)E40/sina³ + flies.

(Yamamoto et al., 1996)

Amorph based on the phenotypes of hemizygous <up>sina¹/Df(3L)st-g18</up> versus that of homozygous flies.

(Lindsley and Zimm, 1992)

This allele probably represents complete loss of function of the sina gene. In the ommatidia the R7 photoreceptor cell is always missing, the R7 precursor adopting a cone cell fate, and sometimes one or two cells of the R1-R6 class are missing as well. The normal trapezoidal arrangement of the R1-R6 cells is disorganised, and the R8 cell is often found in uncharacteristic positions. Other adult sensory organs are also affected: sensory bristles distributed over the body surface are frequently missing, with neither the cuticular bristle sheath nor the socket being present, affected sensory organs including the microchaetae and macrochaetae of the head, notum, abdomen and wing margin, and the stout and slender bristles of the anterior wing margin. The phenotype is most severe with bristles located on the abdomen, where often only a minor fraction of the normal number of bristles are present. Also, many of the remaining bristles have two or three sheaths emerging from a single socket. There is also a 10-fold reduction in adult lifespan, and adult behaviour is generally lethargic and uncoordinated. Neither males nor females are fertile, although they produce morphologically normal sperm and eggs, respectively.

(Carthew and Rubin, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sina³/sina² has visible phenotype, enhanceable by msi¹/msi¹

(Okabe et al., 2001)

sina³/sina² has visible phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has visible phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

Suppressed by

Statement

Reference

sina³/sina² has visible phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

Enhancer of

Statement

Reference

sina³/sina² is an enhancer of visible | recessive phenotype of msi¹

(Okabe et al., 2001)

sina³/sina² is an enhancer of visible phenotype of msi¹

(Hirota et al., 1999)

NOT Enhancer of

Statement

Reference

sina³ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

sina³ is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sina³ is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

sina³ is a suppressor of visible phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

sina³ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

sina³ is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sina³ is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

sina³/sina² has photoreceptor cell R1 | third instar larval stage phenotype, enhanceable by msi¹/msi¹

(Siddall et al., 2009)

sina³/sina² has photoreceptor cell R6 | third instar larval stage phenotype, enhanceable by msi¹/msi¹

(Siddall et al., 2009)

sina³/sina² has photoreceptor cell R7 | third instar larval stage phenotype, enhanceable by msi¹/msi¹

(Siddall et al., 2009)

sina³/sina² has external sensory organ phenotype, enhanceable by msi¹/msi¹

(Okabe et al., 2001)

sina³/sina² has adult abdominal segment 3 & chaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & chaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & tormogen cell | supernumerary phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & trichogen cell | supernumerary phenotype, enhanceable by phyl[+]/phyl²²⁴⁵

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & chaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & chaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & tormogen cell | supernumerary phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & trichogen cell | supernumerary phenotype, enhanceable by phyl²/phyl[+]

(Pi et al., 2001)

Suppressed by

Statement

Reference

msi¹, sina³/sina² has photoreceptor cell R1 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

msi¹, sina³/sina² has photoreceptor cell R6 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

msi¹, sina³/sina² has photoreceptor cell R7 | third instar larval stage phenotype, suppressible by ttk^GD4414/Scer\GAL4^lz-gal4

(Siddall et al., 2009)

sina³/sina² has photoreceptor cell R7 phenotype, suppressible by ed^slH8/ed^1X5

(Bai et al., 2001)

sina³/sina² has ommatidium phenotype, suppressible by ed^slH8/ed^1X5

(Bai et al., 2001)

sina³/sina² has adult abdominal segment 3 & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & chaeta phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & microchaeta phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 4 & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & chaeta phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has mesothoracic tergum & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 3 & chaeta phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 3 & microchaeta phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

sina³/sina² has adult abdominal segment 3 & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttk^osn

(Pi et al., 2001)

msi¹, sina³/sina² has ommatidium phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

msi¹, sina³/sina² has rhabdomere phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

msi¹, sina³/sina² has cone cell phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

sina³ has phenotype, suppressible by ttk^osn

(Hirota et al., 1999)

Enhancer of

Statement

Reference

sina³/sina² is an enhancer of external sensory organ phenotype of msi¹

(Okabe et al., 2001)

sina³/sina² is an enhancer of ommatidium phenotype of msi¹

(Hirota et al., 1999)

NOT Enhancer of

Statement

Reference

sina³ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

sina³ is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sina³ is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

sina³/sina² is a suppressor of eye phenotype of phyl^GMR.PC

(Tang et al., 1997)

sina³ is a suppressor of phenotype of phyl^hs.sev

(Tang et al., 1997)

sina³ is a suppressor of eye phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

sina³ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

sina³ is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

sina³ is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Other

Statement

Reference

msi¹, sina³/sina², ttk^osn has ommatidium phenotype

(Hirota et al., 1999)

msi¹, sina³/sina², ttk^osn has cone cell phenotype

(Hirota et al., 1999)

msi¹, sina³/sina², ttk^osn has rhabdomere phenotype

(Hirota et al., 1999)

sina¹/sina³, svp^2.sev has photoreceptor cell R7 phenotype

(Hiromi et al., 1993)

sina¹/sina³, svp^1.sev has photoreceptor cell R7 phenotype

(Hiromi et al., 1993)

Additional Comments

Genetic Interactions

Statement

Reference

Double mutants of sina²/sina³ with msi¹ show a synergistic, not additive, eye phenotype. Ommatidial arrays are disturbed, eyes consequently roughened and ommatidia do not contain more than five rhabdomeres. Cone cell array is disturbed. 30% of the ommatidia of sina² msi¹ ttk^osn/sina³ msi¹ show the normal number and arrangement of photoreceptor cells. The cone cell defects of sina² msi¹/sina³ msi¹ are also suppressed by ttk^osn.

(Hirota et al., 1999)

The phyl^GMR.PC eye phenotype is completely suppressed in a sina²/sina³ background; the eyes are normal, except for the lack of R7 photoreceptor cells due to the absence of sina function. sina²/sina³ flies carrying both phyl^GMR.PC and ttk^{88.GMR.T:Hsap\MYC} have very disrupted eyes, similar to those of flies carrying ttk^{88.GMR.T:Hsap\MYC} alone.

(Tang et al., 1997)

sina¹/sina³; svp^1.sev and sina¹/sina³; svp^2.sev flies differentiate neither normal nor ectopic R7 cells.

(Hiromi et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

5780

st¹ sina³/TM6B, Tb¹

26270

w^*; sina³ P{neoFRT}80B/TM3, Sb¹

Kyoto

108422

st¹ sina³/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

No interaction with P{sev-svp1} or P{sev-svp2} exists.

(Begemann et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sina³

(Cooper, 2007)

sina³

(Hughes et al., 2019, Yin and Xi, 2018, Birkholz et al., 2009, Shi and Noll, 2009, Siddall et al., 2009)

sina^A16

(Lindsley and Zimm, 1992)

Name Synonyms

Secondary FlyBase IDs

FBal0056160

References (27)

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