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General Information
Symbol
Dmel\sisA1
Species
D. melanogaster
Name
FlyBase ID
FBal0015671
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sis-a, sis-a1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
A11321909G
Reported nucleotide change:
A6547G
Amino acid change:
K128E | sisA-PA
Reported amino acid change:
K128E
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Lys to Gln substitution within the DNA binding motif.
Amino acid replacement: K128E. Nucleotide substitution: A547G. Mutation is in a moderately conserved position of the DNA binding domain.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
sc10-1/sisA1 female transheterozygotes have reduced viability, they are fully viable when they carry P{scsub}.
scsisB-1; l(1)schb.PP and sisA1; schb.PP embryos lack posterior Sxl expression.
Interacts with RpII140wimp maternal effect.
Female embryos hemizygous for sisA1 show 100% lethality.
Homozygous females die but hemizygous males and heterozygous females fully viable; females die as embryos and larvae; rare morphologically normal and fertile escapers observed at lower temperatures. Single extant allele hypomorphic; locus also defined by dominant behavior of deficiencies and duplications. Dominant lethal for females simultaneously heterozygous for sis-b- or Sxl- or whose mothers are heterozygous for da-. Magnitude of female-lethal dominant synergism sensitive to genetic background, but can be very high. Lethal interactions are generally less severe at lower culture temperatures. Constitutive allele, SxlM1, suppresses female lethality of sis-a homozygote or of any heterozygous combination of mutant alleles or deficiencies of these four genes. Duplication of Sxl+ also suppresses, but less effectively. Female-lethal interactions between sis-a and Sxl mutations display remarkably similar Sxl allele specificity to those between maternal da and zygotic Sxl mutations, indicating that da and sis-a disrupt the same aspect of Sxl regulation. Oogenesis normal for homozygous sis-a germ-line clones induced by mitotic recombination; no maternal effect in homozygous germ-line clones. Sexual phenotype of 2X:3A animals extremely sensitive to sis-a+ dose (more male at lower temperatures) and like da and Sxl-, shows masculinizing interaction with autosomal male-specific lethal mutations but no increase in viability of escapers; nevertheless, sexual phenotype of homozygous sis-a clones generated by mitotic recombination normal. Female-lethal effects caused by decrease in sis-a function have their complement in male-lethal interactions caused by increase in sis-a function (duplications). Male lethality is increased as Sxl+ dose or sis-b+ dose is increased and is suppressed by loss-of-function Sxl mutations. The dose-dependent interactions of this gene with Sxl+ identify it as part of the numerator of what has been called the X/A balance, the primary sex-determination signal--a character it shares with sis-b.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
msl-3[+]/msl-31, scsisB-1, sisA[+]/sisA1 has lethal | dominant | female | maternal effect phenotype, suppressible | maternal effect | partially by SxlM1/Sxl[+]
sisA1/Df(1)N71 has lethal | female phenotype, suppressible by SxlM4
sisA2/sisA1 has lethal | female phenotype, suppressible by SxlM4
sisA1 has lethal | female phenotype, suppressible by dpn[+]/dpn3
sisA1 has lethal | female phenotype, suppressible by dpn[+]/dpn4
sisA1 has lethal | female phenotype, suppressible by dpn5/dpn[+]
sisA1 has lethal | female phenotype, suppressible by dpn[+]/dpn6
sisA1 has lethal | female phenotype, suppressible by dpn7/dpn[+]
sisA1 has lethal | female phenotype, suppressible by dpn8/dpn[+]
sisA1 has lethal | female phenotype, suppressible by scB5
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
scsisB-1 sisA1/+ females show reduced viability compared to their male siblings. One maternally-derived copy of either mle1, msl-1L60, msl-21 or msl-31 significantly reduces the viability of scsisB-1 sisA1/+ females so that they show almost complete lethality. One paternally-derived copy of either mle1, msl-1L60, msl-21 or msl-31 significantly reduces the viability of scsisB-1 sisA1/+ females, although this zygotic effect is generally weaker than the maternal one. The viability of sisA1/+ females is significantly reduced if they also carry one maternally-derived copy of msl-31. One paternally-derived copy of mof2, roX1ex6 or roX2unspecified reduces the viability of scsisB-1 sisA1/+ females. The almost complete lethality of scsisB-1 sisA1/+ females carrying a maternally-derived copy of msl-31 is significantly rescued (to 72.8% viability) by a maternally-derived copy of SxlM1.
Maternal heterozygosity for Bap601 reduces the viability of female offspring which are doubly heterozygous for sisA1 upd1sisC-1. Maternal heterozygosity for Bap601 reduces the viability of female offspring which are heterozygous for sisA1.
Does not interact with Sxldlf. Females doubly heterozygous for sisA1 and either fl(1)3535 or fl(1)3546 show reduced viability compared to single heterozygous females. Females doubly heterozygous for l(1)4343 and sisA1 show reduced viability compared to single heterozygous females.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially complements
Comments
sisA4 partially complements the female lethality of sisA1 (2.6% of sisA1/sisA4 females survive).
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Synonyms and Secondary IDs (3)
References (18)