Polytene chromosomes normal.
When compared with single mutants, the combined mutational effects of sei1/slo1 are not simply additive, with a further enhancement of some slo, but not sei phenotypes. Satellite abundance is less extreme in sei1/slo1 double mutants compared to sei1 mutants. There is a slight, but not significant, reduction in the numbers of both types B and M satellites. In contrast, the numbers of type M (but not type B) satellites and mature boutons are significantly greater in double mutants compared to slo single mutants.
The frequency of satellite formation in Fas2e76; slo1 double mutants is comparable to slo1 single mutants. Overall bouton number is slightly reduced in these double mutants. However, a similar reduction is seen in Fas2e76 mutants.
dlg17/slo1 mutants exhibit reduced levels of type B and type M satellites, close to wild-type levels, compared to slo1 single mutants. This reduction correlates with reduced levels of mature boutons and with simpler branching patterns.
A Ca-α1DAR66 mutant background suppresses both type B and type M satellites in Ca-α1DAR66; slo1 double mutants nearly to wild-type levels. Such suppression in satellite formation leads to the morphology of these double mutants to resemble Ca-α1DAR66 single mutants.
Overexpression of dlg1WT.Scer\UAS.T:Avic\GFP-EGFP in the developing muscle under the control of Scer\GAL4Mef2.247 induces a more pronounced reduction in type B satellites (small boutons) in slo1 mutants. As a result, the numbers of remaining type B and type M satellites becomes similar, and there are fewer satellites in total. The number of mature boutons is significantly enhanced in slo1 larvae expressing post-synaptic dlg1WT.Scer\UAS.T:Avic\GFP-EGFP.
Despite their abundance in slo1 single mutants, levels of both type B and type M satellites are suppressed in rut1; slo1 double mutants to wild-type levels, along with a slight reduction in mature bouton number.
Post-synaptic expression of dncScer\UAS.cCa (under the control of Scer\GAL4C164 in a slo1 background suppresses the number of both type M and type B satellites. However, the number of mature boutons remains higher in these mutants.