Allele Dmel\sn^X2

General Information
Symbol	Dmel\sn^X2	Species	D. melanogaster
Name	from X irradiation	FlyBase ID	FBal0015886
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\sn

Allele class
Mutagen	X ray
Nature of the Allele
Allele class
Mutagen	X ray (Roiha et al., 1988, Cant et al., 1994)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 0.1 kb insert in -1.9 to -1.2 same insert present in parental chromosome; coordinates according to Roiha, Rubin and O'Hare (1988) Insertion of 0.1kb DNA, at coordinates -1.9 to -1.2. (Roiha et al., 1988) The 0.1kb insertion between -1.2 and -1.9 (coordinates as in FBrf0048245) is present in the parental In(1)dl-49 chromosome and is a polymorphism rather than the cause of the mutant phenotype. (Paterson and O'Hare, 1991)
Assay mode
Carried on aberration	FM7c-vHa (FlyBase Curators, 2006-) FM7c-FTG (FlyBase Curators, 2006-) FM7c-actLacZ (FlyBase Curators, 2006-) FM7c-vKa (FlyBase Curators, 2006-) FM7c-10 (FlyBase Curators, 2006-) FM7c-34 (FlyBase Curators, 2006-) FM7k (FlyBase Curators, 2006-, Bloomington Drosophila Stock Center, 1998.5.21) Binsn (FlyBase Curators, 2006-, Lindsley and Zimm, 1992) FM7c-ct^S (FlyBase Curators, 2006-) FM7c (FlyBase Curators, 2006-, Lindsley and Zimm, 1992) FM7c-B⁺ (FlyBase Curators, 2006-) Binsinscy (FlyBase Curators, 2006-, Lindsley and Zimm, 1992) FM7c-w⁺ (FlyBase Curators, 2006-)
Cytology
Phenotypic Data
Phenotypic Class
	visible (O'Hare et al., 1998, Dickinson and Thatcher, 1997, Paterson and O'Hare, 1991) sterile (Cant et al., 1994) female sterile (O'Hare et al., 1998, Roiha et al., 1988, Paterson and O'Hare, 1991) female sterile \| recessive (Guild et al., 1997) male fertile (Paterson and O'Hare, 1991)
Phenotype Manifest In
	macrochaeta (Cant et al., 1994, O'Hare et al., 1998, Roiha et al., 1988, Paterson and O'Hare, 1991) actin filament & egg chamber (Wahlstrom et al., 2004) denticle belt (Dickinson and Thatcher, 1997) microchaeta (Dickinson and Thatcher, 1997) macrochaeta & actin filament (Tilney et al., 1998) egg (Guild et al., 1997) nurse cell ring canal (Guild et al., 1997)
Detailed Description
	Statement Reference class 1 - female sterile with gnarled macrochaetae and kinky microchaetae sn^X2/sn^MR39B1 flies are sterile. (Eeken, 1982) Extreme bristle phenotype, female sterile. (Roiha et al., 1988) Extreme bristle phenotype. (Paterson and O'Hare, 1991) Repression potential of a P-element repressor protein in a singed sterility assay of sn^w/sn^X2 heterozygotes shows partial correspondence with gonadal dysgenic sterility in that strong repression of gonadal dysgenic sterility shows some effect in the singed sterility assay. (Misra et al., 1993) Bristles are gnarled due to disrupted actin filament bundles. Nurse cell cytoplasmic actin filaments are absent. (Cant et al., 1994) Does not show premature cytoplasmic streaming in stage 8 egg chambers. (Manseau et al., 1996) Mutation causes a severe denticle phenotype. Denticles tend to be smaller and have thinner hooks than wild-type denticles. They are also less orderly along each row and sometimes have a floppy appearance. Some hairs are shorter and thicker than normal. (Dickinson and Thatcher, 1997) Homozygous females produce sterile eggs. Ring canal morphology in the egg chambers of homozygous females ranges from nearly wild-type to very disorganised. The nurse cell nuclei of late stage follicles are lobed and almost wild-type in morphology. Individual lobes of these nuclei are seen to block the ring canals in these follicles. (Guild et al., 1997) Strong bristle phenotype. (O'Hare et al., 1998) Apoptotic nuclei are seen in sn^X2 egg chambers, although the pattern of apoptosis appears somewhat different to wild-type. (Cavaliere et al., 1998) Tiny bundles of actin appear near the plasma membrane at the tip of elongating sn^X2 bristles, as in the wild-type. These bundles aggregate into ribbon-shaped aggregations of filaments adjacent to the plasma membrane. (Tilney et al., 1998) Homozygous egg chambers contain short actin bundles compared to wild-type. (Wahlstrom et al., 2004)
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Statement Reference sn^X2 has actin filament & egg chamber phenotype, non-enhanceable by Actn^Δ233 (Wahlstrom et al., 2004)
NOT suppressed by
Statement Reference sn^X2 has actin filament & egg chamber phenotype, non-suppressible by Actn^Δ233 (Wahlstrom et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference The short actin bundles seen in the egg chambers of sn^X2 single mutants are also seen in Actn^Δ233 sn^X2/Actn^Δ233 sn^X2 double mutant egg chambers. (Wahlstrom et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 27 )
Bloomington	1035 In(1)Mud, Mud¹/In(1)dl-49, sn^X2 6220 Df(1)y-X15/In(1)dl-49, v¹ sn^X2 mal² 3753 In(1)dl-49, y¹ sn^X2 bb^l/In(1)sc⁸, Df(1)mal, sc⁸
Kyoto	108683 Df(1)y-X15/In(1)dl-49, v¹ sn^X2 mal² 107224 In(1)dl-49, y¹ sn^X2 bb^l/In(1)sc⁸, Df(1)mal, sc⁸ 100974 Binsn, sc⁸ sc^S1 sn^X2 B¹ / unc² / Dp(1;Y)mal⁺ 103245 Df(1)JC19 / FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ 103246 Df(1)JC70 / FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ 101090 eo²⁷ / FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ 101093 fliI⁸ / FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ 101113 FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ / unc¹⁰ 100956 FM7, y^31d sc⁸ w^a sn^X2 v^Of g⁴ B¹ / Hlc^19Ff-6
	More stocks available...
Notes on Origin
Discoverer	Muller.

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	singed^X2 (Guild et al., 1997) sn^x2 (Cavaliere et al., 1998, Manseau et al., 1996) sn^X2
Name Synonym	from X irradiation
Secondary FlyBase IDs

References ( 21 )

Generate a list of	All references relating to this allele ( 21 )

List References by type	Research paper ( 16 ) Personal communication to FlyBase ( 1 ) Abstract ( 1 ) FlyBase analysis ( 1 ) Book ( 1 ) Stock list ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2006 Show research papers ...

Allele Dmel\snX2

Allele Dmel\sn^X2