/+ nurse cells, the nucleolus has a dispersed phenotype and fewer interconnected tubules than controls. The severity of the phenotype is lower at oogenesis stages 8, 9 and 10A compared to 10B, and in anterior nurse cells.
mutant terminal cells are morphologically indistinguishable from wild type. No apparent alteration of actin organisation in terminal cells is seen.
Stage 14 snX2
embryos show a substantial delay in the migration of plasmatocytes along the ventral midline. 31% of these embryos show "strong" migration defects. These defects persist at later stages - plasmatocytes remain aggregated in the ventral part of the embryo and are unable to reach the posterior region.
The number of plasmatocytes recruited to a laser-induced wound is significantly reduced in snX2
mutants compared to controls. The migratory speed of snX2
plasmatocytes is also reduced.
neurons cultured from snX2
brains have a distinctive abnormal morphology. Axons curl, creating a filagree pattern. Filamentous axon distribution within these neurons is abnormal.
mutant bristles lack fascin crossbridges but still form microvilli. Pimple and microvilli in this mutant appear not only along the epithelial cells between the bristle shafts but are also prominent on the new emerging bristle. A linear array of tiny actin bundles is found attached to the plasma membrane. Each of these contains approximately the same number of filaments as those in the microvilli on the bristle tip or to those in longitudinal section.
Homozygous egg chambers contain short actin bundles compared to wild-type.
Apoptotic nuclei are seen in snX2
egg chambers, although the pattern of apoptosis appears somewhat different to wild-type.
Strong bristle phenotype.
Tiny bundles of actin appear near the plasma membrane at the tip of elongating snX2
bristles, as in the wild-type. These bundles aggregate into ribbon-shaped aggregations of filaments adjacent to the plasma membrane.
Mutation causes a severe denticle phenotype. Denticles tend to be smaller and have thinner hooks than wild-type denticles. They are also less orderly along each row and sometimes have a floppy appearance. Some hairs are shorter and thicker than normal.
Homozygous females produce sterile eggs. Ring canal morphology in the egg chambers of homozygous females ranges from nearly wild-type to very disorganised. The nurse cell nuclei of late stage follicles are lobed and almost wild-type in morphology. Individual lobes of these nuclei are seen to block the ring canals in these follicles.
Does not show premature cytoplasmic streaming in stage 8 egg chambers.
Bristles are gnarled due to disrupted actin filament bundles. Nurse cell cytoplasmic actin filaments are absent.
Repression potential of a P-element
repressor protein in a singed sterility assay of snw
heterozygotes shows partial correspondence with gonadal dysgenic sterility in that strong repression of gonadal dysgenic sterility shows some effect in the singed sterility assay.
Extreme bristle phenotype.
Extreme bristle phenotype, female sterile.
class 1 - female sterile with gnarled macrochaetae and kinky microchaetae