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General Information
Symbol
Dmel\sna1
Species
D. melanogaster
Name
FlyBase ID
FBal0015887
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
snaHG31, HG31
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T15477253A

Reported nucleotide change:

T?A

Amino acid change:

C285term | sna-PA

Reported amino acid change:

C285term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Nucleotide substitution: T?A.

The premature stop codon is in the second zinc finger.

Amino acid replacement: C285term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

sna1/Df(2L)ED1054 transheterozygotes present significant decreases in the number of dividing neuroblasts and dividing neuroblast daughters during stage 14 of embryogenesis, as compared to controls.

sna1/sna18;snaSquish mutant embryos exhibit normal gastrulation.

In sna1/sna18;snaSquish mutant embryos the gland primordia degenerate and disappear.

sna1 in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces viable flies.

Very little ventral cell invagination is seen by mid to late germ band extension.

Ventral furrow fails to form.

Strong allele.

sna1/Tp(2;1)Scorv23 adult escapers are occasionally produced, which often have missing halteres or show a hemithorax phenotype. Homozygous embryos fail to form a ventral furrow. Cephalic furrow formation, invagination of the endoderm and germ band elongation do take place in these embryos. Embryos show varying defects in the segmental denticle belts.

strong allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference

sna1 has phenotype, non-suppressible by eRF1[+]/eRF1F2

NOT Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The addition of a Df(3L)H99 background (blocking apoptosis), results in the survival of the corpora allata and prothoracic glands in sna1/sna18;snaSquish mutant embryos, but they do not undergo epithelial-mesenchymal transition. As a result, the gland primordia maintain epithelial polarity, do not migrate, and form small pouches that remain attached to the epidermis.

esgG66, sna1 clones that express both wordsRNA.842.Scer\UAS and wordsRNA.1701.Scer\UAS, under the control of Scer\GAL4Act5C.PP, in the eye show ectopic photoreceptor and cone cells and a significant reduction of the pigment rim.

esgG66, sna1 clones show the same loss of 2o and 3o pigment cells as esgG66 clones. This phenotype is not enhanced in esgG66, sna1 Scer\GAL4Act5C.PP>wordsRNA.842.Scer\UAS, wordsRNA.1701.Scer\UAS clones.

The wing disc is absent in esgG66, sna1 double mutants, and the apical constrictions do not occur in the wing primordium cells. The haltere discs are similarly affected, but leg discs are unaffected. There is no sign of cell death in the prospective wing disc of the double mutant embryo, though massive cell death in the endoderm due to the sna1 is clearly visible. The mutant phenotypes can be rescued by either snahs.PF or esghs.PF.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Partially rescued by

sna18/sna1 is partially rescued by sna24.8.GFP

sna18/sna1 is partially rescued by snaΔProx

Not rescued by
Comments

At 25[o]C, snaΔProx rescues 82% of sna1/sna18 animals to viability. At 18[o]C, 94% are rescued and at 29[o]C 36% are rescued.

At 25[o]C, sna+t24.8 rescues 91% of sna1/sna18 animals to viability. At 18[o]C, 100% are rescued and at 29[o]C 100% are rescued.

snaSquish and snaDtoP each rescue the gastrulation defects of sna1/sna18 embryos, but the animals are not rescued to adult viability.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Harrington.

M. Ashburner.

Comments
Comments

Phenotypic data included in FBrf0051973.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (28)