FlyBase Allele Report: Dmel\sna[1]

General Information

Symbol

Dmel\sna¹

Species

D. melanogaster

Name

FlyBase ID

FBal0015887

Feature type

allele

Associated gene

Dmel\sna

Associated Insertion(s)

Carried in Construct

Also Known As

sna^HG31, HG31

Key Links

Genomic Maps

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Help me choose

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Fuse et al., 1996)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Hemavathy et al., 1997, Roote, 1995.5.4, Tearle and Nusslein-Volhard, 1987, Grau et al., 1984, Simpson, 1983)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

2L:15,477,253..15,477,253 [-]

Nucleotide change:

T15477253A

Reported nucleotide change:

T?A

Amino acid change:

C285term | sna-PA

Reported amino acid change:

C285term

(Hemavathy et al., 1997)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Simpson, 1983)

Nature of the lesion

Statement

Reference

Nucleotide substitution: T?A.

(Hemavathy et al., 1997)

The premature stop codon is in the second zinc finger.

(Hemavathy et al., 1997)

Amino acid replacement: C285term.

(Hemavathy et al., 1997)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | embryonic stage 14 (with Df(2L)ED1054)

(Bahrampour et al., 2017)

lethal | recessive

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Grau et al., 1984)

lethal - all die during embryonic stage | recessive

(Ashburner et al., 1990)

some die during embryonic stage | recessive

(Ashburner et al., 1990)

Phenotype Manifest In

adult corpus allatum, with sna¹⁸, sna^Squish

(Sánchez-Higueras et al., 2014)

adult corpus allatum (with sna¹⁸), with sna^Squish

(Sánchez-Higueras et al., 2014)

adult thorax

(Grau et al., 1984)

denticle belt

(Grau et al., 1984)

embryo | gastrula stage

(Hemavathy et al., 1997)

embryonic ganglion mother cell | embryonic stage 14 (with Df(2L)ED1054)

(Bahrampour et al., 2017)

embryonic neuroblast | embryonic stage 14 (with Df(2L)ED1054)

(Bahrampour et al., 2017)

endoderm

(Fuse et al., 1996)

haltere

(Grau et al., 1984)

prothoracic gland, with sna¹⁸, sna^Squish

(Sánchez-Higueras et al., 2014)

prothoracic gland (with sna¹⁸), with sna^Squish

(Sánchez-Higueras et al., 2014)

ventral furrow

(Grau et al., 1984)

Detailed Description

Statement

Reference

sna¹/Df(2L)ED1054 transheterozygotes present significant decreases in the number of dividing neuroblasts and dividing neuroblast daughters during stage 14 of embryogenesis, as compared to controls.

(Bahrampour et al., 2017)

sna¹/sna¹⁸;sna^Squish mutant embryos exhibit normal gastrulation.

In sna¹/sna¹⁸;sna^Squish mutant embryos the gland primordia degenerate and disappear.

(Sánchez-Higueras et al., 2014)

sna¹ in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces viable flies.

(Sawamura et al., 2000)

Very little ventral cell invagination is seen by mid to late germ band extension.

(Hemavathy et al., 1997)

Ventral furrow fails to form.

(Ashburner et al., 1990)

Strong allele.

(Boulay et al., 1987)

sna¹/Tp(2;1)Sco^rv23 adult escapers are occasionally produced, which often have missing halteres or show a hemithorax phenotype. Homozygous embryos fail to form a ventral furrow. Cephalic furrow formation, invagination of the endoderm and germ band elongation do take place in these embryos. Embryos show varying defects in the segmental denticle belts.

(Grau et al., 1984)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

sna¹ has phenotype, non-suppressible by eRF1[+]/eRF1^F2

(Chao et al., 2003)

NOT Enhancer of

Statement

Reference

sna¹ is a non-enhancer of secondary pigment cell | somatic clone phenotype of esg^G66

(Lim and Tomlinson, 2006)

sna¹ is a non-enhancer of tertiary pigment cell | somatic clone phenotype of esg^G66

(Lim and Tomlinson, 2006)

wor^{dsRNA.1701.UAS}, wor^{dsRNA.842.UAS}, sna¹, Scer\GAL4^Act5C.PP is a non-enhancer of secondary pigment cell | somatic clone phenotype of esg^G66

(Lim and Tomlinson, 2006)

wor^{dsRNA.1701.UAS}, wor^{dsRNA.842.UAS}, sna¹, Scer\GAL4^Act5C.PP is a non-enhancer of tertiary pigment cell | somatic clone phenotype of esg^G66

(Lim and Tomlinson, 2006)

Other

Statement

Reference

Df(3L)H99, sna¹⁸/sna¹, sna^Squish has adult corpus allatum phenotype

(Sánchez-Higueras et al., 2014)

Df(3L)H99, sna¹⁸/sna¹, sna^Squish has prothoracic gland phenotype

(Sánchez-Higueras et al., 2014)

Df(3L)H99, sna¹⁸, sna¹, sna^Squish has adult corpus allatum phenotype

(Sánchez-Higueras et al., 2014)

Df(3L)H99, sna¹⁸, sna¹, sna^Squish has prothoracic gland phenotype

(Sánchez-Higueras et al., 2014)

Scer\GAL4^Act5C.PP, esg^G66, sna¹, wor^{dsRNA.1701.UAS}, wor^{dsRNA.842.UAS} has eye photoreceptor cell | ectopic | somatic clone phenotype

(Lim and Tomlinson, 2006)

Scer\GAL4^Act5C.PP, esg^G66, sna¹, wor^{dsRNA.1701.UAS}, wor^{dsRNA.842.UAS} has cone cell | ectopic | somatic clone phenotype

(Lim and Tomlinson, 2006)

Scer\GAL4^Act5C.PP, esg^G66, sna¹, wor^{dsRNA.1701.UAS}, wor^{dsRNA.842.UAS} has pigment cell | somatic clone phenotype

(Lim and Tomlinson, 2006)

esg^G66, sna¹, wor^dsRNA.cCa has neuroblast phenotype

(Cai et al., 2003)

esg^G66, sna¹ has wing disc phenotype

(Fuse et al., 1996)

esg^G66, sna¹ has haltere disc phenotype

(Fuse et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of a Df(3L)H99 background (blocking apoptosis), results in the survival of the corpora allata and prothoracic glands in sna¹/sna¹⁸;sna^Squish mutant embryos, but they do not undergo epithelial-mesenchymal transition. As a result, the gland primordia maintain epithelial polarity, do not migrate, and form small pouches that remain attached to the epidermis.

(Sánchez-Higueras et al., 2014)

esg^G66, sna¹ clones that express both wor^{dsRNA.842.Scer\UAS} and wor^{dsRNA.1701.Scer\UAS}, under the control of Scer\GAL4^Act5C.PP, in the eye show ectopic photoreceptor and cone cells and a significant reduction of the pigment rim.

esg^G66, sna¹ clones show the same loss of 2^o and 3^o pigment cells as esg^G66 clones. This phenotype is not enhanced in esg^G66, sna¹ Scer\GAL4^Act5C.PP>wor^{dsRNA.842.Scer\UAS}, wor^{dsRNA.1701.Scer\UAS} clones.

(Lim and Tomlinson, 2006)

The wing disc is absent in esg^G66, sna¹ double mutants, and the apical constrictions do not occur in the wing primordium cells. The haltere discs are similarly affected, but leg discs are unaffected. There is no sign of cell death in the prospective wing disc of the double mutant embryo, though massive cell death in the endoderm due to the sna¹ is clearly visible. The mutant phenotypes can be rescued by either sna^hs.PF or esg^hs.PF.

(Fuse et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sna²

(Grau et al., 1984)

sna³

(Grau et al., 1984)

sna⁴

(Grau et al., 1984)

sna⁵

(Grau et al., 1984)

sna⁶

(Grau et al., 1984)

sna¹⁸

(Grau et al., 1984)

sna^Sco-rv15

(Grau et al., 1984)

Rescued by

sna¹⁸/sna¹ is rescued by sna^+t24.8

(Dunipace et al., 2011)

sna¹ is rescued by sna^hs.PF

(Fuse et al., 1996)

Partially rescued by

sna¹⁸/sna¹ is partially rescued by sna^24.8.GFP

(Dunipace et al., 2011)

sna¹⁸/sna¹ is partially rescued by sna^ΔProx

(Dunipace et al., 2011)

Not rescued by

sna¹⁸/sna¹ is not rescued by sna^ΔDistal

(Dunipace et al., 2011)

sna¹⁸/sna¹ is not rescued by sna^ΔPD

(Dunipace et al., 2011)

sna¹⁸/sna¹ is not rescued by sna^Squish

(Dunipace et al., 2011)

Comments

At 25[o]C, sna^ΔProx rescues 82% of sna¹/sna¹⁸ animals to viability. At 18[o]C, 94% are rescued and at 29[o]C 36% are rescued.

At 25[o]C, sna^+t24.8 rescues 91% of sna¹/sna¹⁸ animals to viability. At 18[o]C, 100% are rescued and at 29[o]C 100% are rescued.

sna^Squish and sna^DtoP each rescue the gastrulation defects of sna¹/sna¹⁸ embryos, but the animals are not rescued to adult viability.

(Dunipace et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

25127

Adhⁿ⁷ sna¹ cn¹ vg¹/CyO

Kyoto

101290

Adhⁿ⁷ sna¹ cn¹ (vg¹) / CyO, Duox^Cy dpy^lv1 pr¹ cn²

Notes on Origin

Discoverer

Harrington.

M. Ashburner.

(Simpson, 1983)

Comments

Phenotypic data included in FBrf0051973.

(Roote, 1995.5.4)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

HG31

(Hemavathy et al., 1997, Roote, 1995.5.4, Boulay et al., 1987, Ashburner et al., 1983)

l(2)br28^HG31

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna)

sna¹

(Arbouzova et al., 2019, Bahrampour et al., 2017, Sánchez-Higueras et al., 2014, Dunipace et al., 2011, Kuzin et al., 2011, Roote, 2010.1.4, Lim and Tomlinson, 2006)

sna^HG31

(Ganguly et al., 2005, Ashraf and Ip, 2001, Sawamura et al., 2000, Ashraf et al., 1999, Nguyen et al., 1994, Ashburner et al., 1990, Bodmer et al., 1990, Gubb et al., 1988, Grau et al., 1984, Simpson, 1983)

sna^HG

(Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (29)

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