T15477253A
T?A
C285term | sna-PA
C285term
Polytene chromosomes normal.
Nucleotide substitution: T?A.
The premature stop codon is in the second zinc finger.
Amino acid replacement: C285term.
adult corpus allatum (with sna18), with snaSquish
prothoracic gland (with sna18), with snaSquish
sna1/Df(2L)ED1054 transheterozygotes present significant decreases in the number of dividing neuroblasts and dividing neuroblast daughters during stage 14 of embryogenesis, as compared to controls.
sna1 in combination with a pair of introgressions from D.simulans spanning 30F1-31E7 to 35D7-36A14 Dsim\Int(2L)S and 21A1 to 22D1--23A2 Dsim\Int(2L)D produces viable flies.
Very little ventral cell invagination is seen by mid to late germ band extension.
Ventral furrow fails to form.
Strong allele.
sna1/Tp(2;1)Scorv23 adult escapers are occasionally produced, which often have missing halteres or show a hemithorax phenotype. Homozygous embryos fail to form a ventral furrow. Cephalic furrow formation, invagination of the endoderm and germ band elongation do take place in these embryos. Embryos show varying defects in the segmental denticle belts.
strong allele
sna1 is a non-enhancer of secondary pigment cell | somatic clone phenotype of esgG66
sna1 is a non-enhancer of tertiary pigment cell | somatic clone phenotype of esgG66
wordsRNA.1701.UAS, wordsRNA.842.UAS, sna1, Scer\GAL4Act5C.PP is a non-enhancer of secondary pigment cell | somatic clone phenotype of esgG66
wordsRNA.1701.UAS, wordsRNA.842.UAS, sna1, Scer\GAL4Act5C.PP is a non-enhancer of tertiary pigment cell | somatic clone phenotype of esgG66
Df(3L)H99, sna18/sna1, snaSquish has adult corpus allatum phenotype
Df(3L)H99, sna18/sna1, snaSquish has prothoracic gland phenotype
Df(3L)H99, sna18, sna1, snaSquish has adult corpus allatum phenotype
Df(3L)H99, sna18, sna1, snaSquish has prothoracic gland phenotype
Scer\GAL4Act5C.PP, esgG66, sna1, wordsRNA.1701.UAS, wordsRNA.842.UAS has eye photoreceptor cell | ectopic | somatic clone phenotype
Scer\GAL4Act5C.PP, esgG66, sna1, wordsRNA.1701.UAS, wordsRNA.842.UAS has cone cell | ectopic | somatic clone phenotype
Scer\GAL4Act5C.PP, esgG66, sna1, wordsRNA.1701.UAS, wordsRNA.842.UAS has pigment cell | somatic clone phenotype
esgG66, sna1, wordsRNA.cCa has neuroblast phenotype
esgG66, sna1 has haltere disc phenotype
The addition of a Df(3L)H99 background (blocking apoptosis), results in the survival of the corpora allata and prothoracic glands in sna1/sna18;snaSquish mutant embryos, but they do not undergo epithelial-mesenchymal transition. As a result, the gland primordia maintain epithelial polarity, do not migrate, and form small pouches that remain attached to the epidermis.
esgG66, sna1 clones that express both wordsRNA.842.Scer\UAS and wordsRNA.1701.Scer\UAS, under the control of Scer\GAL4Act5C.PP, in the eye show ectopic photoreceptor and cone cells and a significant reduction of the pigment rim.
esgG66, sna1 clones show the same loss of 2o and 3o pigment cells as esgG66 clones. This phenotype is not enhanced in esgG66, sna1 Scer\GAL4Act5C.PP>wordsRNA.842.Scer\UAS, wordsRNA.1701.Scer\UAS clones.
The wing disc is absent in esgG66, sna1 double mutants, and the apical constrictions do not occur in the wing primordium cells. The haltere discs are similarly affected, but leg discs are unaffected. There is no sign of cell death in the prospective wing disc of the double mutant embryo, though massive cell death in the endoderm due to the sna1 is clearly visible. The mutant phenotypes can be rescued by either snahs.PF or esghs.PF.
sna18/sna1 is partially rescued by sna24.8.GFP
At 25[o]C, snaΔProx rescues 82% of sna1/sna18 animals to viability. At 18[o]C, 94% are rescued and at 29[o]C 36% are rescued.
At 25[o]C, sna+t24.8 rescues 91% of sna1/sna18 animals to viability. At 18[o]C, 100% are rescued and at 29[o]C 100% are rescued.
snaSquish and snaDtoP each rescue the gastrulation defects of sna1/sna18 embryos, but the animals are not rescued to adult viability.
Harrington.
M. Ashburner.
Phenotypic data included in FBrf0051973.