Polytene chromosomes normal.
The addition of a Df(3L)H99 background (blocking apoptosis), results in the survival of the corpora allata and prothoracic glands in sna1/sna18;snaSquish mutant embryos, but they do not undergo epithelial-mesenchymal transition. As a result, the gland primordia maintain epithelial polarity, do not migrate, and form small pouches that remain attached to the epidermis.
esgG66, sna1 clones that express both wordsRNA.842.Scer\UAS and wordsRNA.1701.Scer\UAS, under the control of Scer\GAL4Act5C.PP, in the eye show ectopic photoreceptor and cone cells and a significant reduction of the pigment rim.
esgG66, sna1 clones show the same loss of 2o and 3o pigment cells as esgG66 clones. This phenotype is not enhanced in esgG66, sna1 Scer\GAL4Act5C.PP>wordsRNA.842.Scer\UAS, wordsRNA.1701.Scer\UAS clones.
The wing disc is absent in esgG66, sna1 double mutants, and the apical constrictions do not occur in the wing primordium cells. The haltere discs are similarly affected, but leg discs are unaffected. There is no sign of cell death in the prospective wing disc of the double mutant embryo, though massive cell death in the endoderm due to the sna1 is clearly visible. The mutant phenotypes can be rescued by either snahs.PF or esghs.PF.