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General Information
Symbol
Dmel\wgspd-fg
Species
D. melanogaster
Name
flag
FlyBase ID
FBal0015993
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
spdflg, spdfg
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Lesion in an enhancer element.
A small deletion in the 5' regulatory region of wg.
Regulatory mutation.
1kb deletion located 9kb 5' of the promoter, in the 5' regulatory region, removing an enhancer element.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
In wgspd-fg mutant third instar larval wing imaginal discs, the prospective distal hinge region is reduced in size, however, there is no increase in apoptotic cells compared to wild-type.
The wing hinge region is deleted in mutant flies and the wing pouch appears directly joined to more proximal cells.
Homozygotes have shortened wings, alula etching (loss of the margin) and occasional etching of the posterior wing margin. Wings are held upward, the 'tent' phenotype. Transheterozygotes with wg alleles show an allele-dependent extent of alular etching and may show the tent phenotype. Transheterozygotes with null wg alleles only show etching of the alar lobe. Loss of Sternopleural function (by wgSp-1 or Df(2L)RF) exaggerates the etched wing phenotype of homozygotes.
wgspd-fg/wgspd-hL2 transheterozygotes exhibit complete loss of anterior and posterior wing margin structures and deletion of wing hinge structures.
Overall reduction in wing size, reduction of the alula and variable loss of posterior wing margin structures. wgl-17/wgspd-fg flies show a milder version of the hinge phenotype seen in wgspd-fg homozygotes. A deficiency that uncovers wg does not exhibit a hinge phenotype. Heterozygotes with wgspd-j2 produce a hinge phenotype indistinguishable from wgspd-fg homozygotes.
Homozygotes have small wings with severely reduced alula and defects at the wing margin. When placed in trans with lethal alleles of wg, some defects in the wing margin occur and the alula is very reduced.
Wings about two-thirds the length and three-fourths the width of wild type, held tentlike over abdomen. Alulae absent or vestigial; proximal posterior wing margins often irregular with tendency to fold under about vein L4. Venation usually normal with occasional blistering. wgspd-fg/spd has phenotype varying from slight shortening of wings to a shape midway between the two homozygotes. Excellent viability and fertility. RK1.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressor of
Statement
Reference
wgspd-fg/wg[+] is a suppressor of visible phenotype of Scer\GAL4Bx-MS1096, hng1KK103642
wgspd-fg is a suppressor | partially of hyperplasia phenotype of ft8/ftG-rv
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
wgspd-fg is a suppressor | partially of wing disc phenotype of ft8/ftG-rv
Other
Additional Comments
Genetic Interactions
Statement
Reference
One copy of wgspd-fg suppresses the wing hinge phenotype seen when hng1KK103642 is expressed under the control of Scer\GAL4Bx-MS1096. No changes in cellular proliferation are detected in these animals.
In wgspd-fg, Sox15KG09145 double mutant third instar larval wing imaginal discs, the distal hinge region is reduced in size compared to either single mutants. The rate of hinge-specific apoptosis is similar in wgspd-fg, Sox15KG09145 double mutant and Sox15KG09145 single mutant L3 wing discs.
Overgrowth of the proximal wing disc in ft8/ftG-rv animals is suppressed by wgspd-fg/wgspd-fg.
Wing phenotype can be partially rescued by Scer\GAL4vg.PM induced expression of dshScer\UAS.cNa, rescue of the anterior wing margin and the posterior wing blade between vein 5 and the posterior margin.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Wing phenotype can be partially rescued by Scer\GAL4vg.PM induced expression of wgScer\UAS.cLa rescue of the anterior wing margin and the posterior wing blade between vein 5 and the posterior margin.
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer
Doane, 14th June 1960.
Comments
Comments
wgspd-fg behaves as a specific regulatory allele of wg in the third instar.
Regulatory mutation of wg that specifically abolishes wg function in the hinge.
Null mutations only cause etching of the alar lobe when in combination with wgspd-fg, but a mutation that affects the upstream sequences (Df(2L)DE) completely fails to complement wgspd-fg demonstrating the lesion responsible is in the upstream sequences.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (20)