Allele Dmel\spn-B¹

General Information
Symbol	Dmel\spn-B¹	Species	D. melanogaster
Name		FlyBase ID	FBal0016033
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\spn-B

Allele class	amorph
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	amorph (Gonzalez-Reyes et al., 1997)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G113R. (Ghabrial et al., 1998)
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	viable (Ghabrial et al., 1998) radiation sensitive (Staeva-Vieira et al., 2003)
Phenotype Manifest In
	synaptonemal complex (Huynh and St. Johnston, 2000) germline cyst (Huynh and St. Johnston, 2000) egg \| dorsal (Gonzalez-Reyes et al., 1997) dorsal appendage (Gonzalez-Reyes et al., 1997) oocyte (Gonzalez-Reyes et al., 1997) karyosome (Gonzalez-Reyes et al., 1997) nurse cell (Gonzalez-Reyes et al., 1997)
Detailed Description
	Statement Reference maternal-effect lethal. Similar to spn-A but normal eggs also recovered; eggs always unfertilized and abnormal eggs often long. Hemizygous eggs exhibit either a strong or weak ventralised phenotype: eggs are longer than wild type and are completely symmetric along the DV axis or the eggs display fused dorsal appendages. Egg chambers of females exhibit a partially penetrant disruption in the positioning of the oocyte which can be located anywhere in the egg chamber. Almost all oocytes lack a karyosome and the oocyte chromosomes are arranged instead in thread-like figures with irregular shape. Germline clones in egg chambers are composed of wild type follicle cells, mutant nurse cells, oocytes that give rise to ventralised eggs and oocytes that lack a karyosome. (Gonzalez-Reyes et al., 1997) Hemizygotes are viable. Eggs derived from fs(1)K10^unspecified; spn-B²/spn-B¹ females show a broad spectrum of phenotypes ranging from completely dorsalised to completely ventralised. spn-B¹/spn-B² larvae show normal sensitivity to methyl methanesulfonate. (Ghabrial et al., 1998) In contrast to wild-type ovaries, where the synaptonemal complex (SC) is always restricted to the oocyte by region 2b, spn-B¹ mutant females show a significant delay in the process, with cysts with more than 1 cell in synapsis in region 3 of the germarium. (Huynh and St. Johnston, 2000) Mutants are weakly sensitive to X ray irradiation when irradiated at either 24-48 or 48-72 hours after egg laying (AEL). Little difference in viability is seen in irradiated at 0-24 hours AEL. (Staeva-Vieira et al., 2003)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference spn-B¹ has radiation sensitive phenotype, non-enhanceable by spn-A^093A (Staeva-Vieira et al., 2003)
NOT suppressed by
Statement Reference spn-B¹ has radiation sensitive phenotype, non-suppressible by spn-A^093A (Staeva-Vieira et al., 2003)
NOT Enhancer of
Statement Reference spn-B¹ is a non-enhancer of radiation sensitive phenotype of spn-A^093A (Staeva-Vieira et al., 2003)
NOT Suppressor of
Statement Reference spn-B¹ is a non-suppressor of radiation sensitive phenotype of spn-A^093A (Staeva-Vieira et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference spn-B¹ has oocyte phenotype, enhanceable by spn-D² (Gonzalez-Reyes et al., 1997)
Enhancer of
Statement Reference spn-B¹ is an enhancer of oocyte phenotype of spn-D² (Gonzalez-Reyes et al., 1997)
Other
Statement Reference mus301⁰⁹⁴, spn-B¹ has oocyte phenotype (Gonzalez-Reyes et al., 1997, Gonzalez-Reyes et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference Double mutants with spn-D² increases the oocyte mispositioning frequency. Double mutant stage 5-6 egg chambers with mus301⁰⁹⁴ exhibit a two-oocyte phenotype: the second pro-oocyte develops as an oocyte rather than a nurse cell. The double mutants delay but do not block the decision between the two pro-oocytes as one of the two cells always becomes a nurse cell eventually. Double mutant mothers with fs(1)K10¹ lay dorsalised eggs, the dorsal appendages form a collar around the entire circumference of the egg. This phenotype is indistinguishable from that of fs(1)K10¹ single mutants. (Gonzalez-Reyes et al., 1997) Has no effect on the frequency of X-Y chromosome nondisjunction seen in Df(1)X-1-53B males. (McKee et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	3324 Dp(1;Y)B^S; ru¹ st¹ spn-B¹ e¹ ca¹/TM3, Sb¹
Kyoto	106978 Dp(1;Y)B^S; ru¹ st¹ spn-B¹ e¹ ca¹/TM3, Sb¹
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	spnB¹ (McKee et al., 2000) spn-B¹ spn-B⁰⁵⁶ (Staeva-Vieira et al., 2003, Findley et al., 2003, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987, ) spnB⁰⁵⁶ (Huynh and St. Johnston, 2000, Ghabrial et al., 1998)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Findley et al., 2003, Development 130(5): 859--871 Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage. [FBrf0155724] Staeva-Vieira et al., 2003, EMBO J. 22(21): 5863--5874 An essential role of DmRad51/SpnA in DNA repair and meiotic checkpoint control. [FBrf0167550] Huynh and St. Johnston, 2000, Development 127(13): 2785--2794 The role of BicD, Egl, Orb and the microtubules in the restriction of meiosis to the Drosophila oocyte. [FBrf0128498] McKee et al., 2000, Genetica 109(1-2): 77--93 On the roles of heterochromatin and euchromatin in meiosis in Drosophila: mapping chromosomal pairing sites and testing candidate mutations for effects on X-Y nondisjunction and meiotic drive in male meiosis. [FBrf0135799] Pare and Suter, 2000, J. Cell Sci. 113(12): 2119--2127 Subcellular localization of bic-D::GFP is linked to an asymmetric oocyte nucleus. [FBrf0128601] Ghabrial et al., 1998, Genes Dev. 12(17): 2711--2723 okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis. [FBrf0104438] Gonzalez-Reyes et al., 1997, Development 124(24): 4927--4937 Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes. [FBrf0100046]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\spn-B1

Allele Dmel\spn-B¹