FlyBase Allele Report: Dmel\spn-E[1]

General Information

Symbol

Dmel\spn-E¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016041

Feature type

allele

Associated gene

Dmel\spn-E

Associated Insertion(s)

Carried in Construct

Also Known As

spn-E⁶¹⁶, hls^E616, spnE¹, spnE⁶¹⁶, spnE^E616

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Gillespie and Berg, 1995)

Mutagen

ethyl methanesulfonate

(Gillespie and Berg, 1995, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: R1081term.

(Ott et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:15,842,858..15,842,858 [+]

Nucleotide change:

C15842858T

Amino acid change:

R1081term | spn-E-PA

Reported amino acid change:

R1081term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ott et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage

(Klattenhoff et al., 2007)

dorsal appendage (with spn-E^hls-Δ125)

(Ott et al., 2014)

egg (with spn-E^hls-Δ125)

(Ott et al., 2014)

egg | dorsal

(Gonzalez-Reyes et al., 1997)

germline cyst

(Huynh and St. Johnston, 2000)

karyosome

(Gonzalez-Reyes et al., 1997)

meiosis & nuclear chromosome

(Stapleton et al., 2001)

meiosis & nuclear chromosome (with spn-E²)

(Stapleton et al., 2001)

meiosis & nuclear chromosome (with spn-E^hls-03987)

(Stapleton et al., 2001)

meiosis & nuclear chromosome (with spn-E^hls-Δ125)

(Stapleton et al., 2001)

meiosis & nuclear chromosome | male

(Stapleton et al., 2001)

oocyte

(Gonzalez-Reyes et al., 1997)

P granule (with spn-E^hls-03987)

(Snee and Macdonald, 2004)

spermatocyte

(Stapleton et al., 2001)

synaptonemal complex

(Huynh and St. Johnston, 2000)

Detailed Description

Statement

Reference

The eggs laid by spn-E¹/spn-E^hls-Δ125 mutant females exhibit moderate dorsoventral patterning defects. The majority of eggs laid are collapsed but many eggs with fused or no dorsal appendages are also seen.

(Ott et al., 2014)

Over half of spn-E¹ eggs show a fused dorsal appendage phenotype and 28% of eggs show no dorsal appendages at all.

(Klattenhoff et al., 2007)

spn-E¹/Df(3R)sbd105 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 38^oC.

(Wei and Rong, 2007)

spn-E²/spn-E¹ larvae are not sensitive to MMS.

(McCaffrey et al., 2006)

Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.

(Pal-Bhadra et al., 2004)

Nuage particles are lost in spn-E¹/spn-E^hls-03987 nurse cells and the perinuclear nuage is smoother than normal.

(Snee and Macdonald, 2004)

82% of progeny derived from homozygous males mated to wild-type females hatch.

(Tomari et al., 2004)

spn-E¹/spn-E² animals show the same survival rate after exposure to 0.08% methyl methanesulfonate as control animals.

(Abdu et al., 2003)

In the primary spermatocytes of mutant males, numerous phenotypes are seen including tripolar meiosis, fragmented chromatin, chromatin bridges, unequal daughter nuclei, incomplete chromatin separation and lagging chromosomes. No unpaired homologs nor prematurely dissociated sister chromatids are observed. A failure in release of cohesion causes abnormal segregation of chromosomes. Primary spermatocytes also have needle-shaped crystals. Homozygotes and trans-heterozygotes with spn-E², spn-E^hls-Δ125 and spn-E^hls-03987 show an increased rate of non-disjunction in all except the small 4th chromosome. There are also substantial biases in recovery of reciprocal sperm classes. Nullo-XY sperm are recovered in considerable excess over the reciprocal XY sperm class. Also a weaker bias in favour of normal X-bearing sperm is seen. spn-E^hls-Δ125/spn-E¹ males that have inherited the spn-E^hls-Δ125 from their mother and spn-E¹ from their father are sterile. Males from the reciprocal cross are fertile.

(Stapleton et al., 2001)

In contrast to wild-type ovaries, where the synaptonemal complex (SC) is always restricted to the oocyte by region 2b, spn-E¹ mutant females show a significant delay in the process, with cysts with more than 1 cell in synapsis in region 3 of the germarium and one cell in synapsis in stage 2 of the vitellarium.

(Huynh and St. Johnston, 2000)

Hemizygous eggs exhibit either a strong or weak ventralised phenotype: eggs are longer than wild type and are completely symmetric along the DV axis or the eggs display fused dorsal appendages. Egg chambers of females exhibit a partially penetrant disruption in the positioning of the oocyte which can be located anywhere in the egg chamber. Almost all oocytes lack a karyosome and the oocyte chromosomes are arranged instead in thread-like figures with irregular shape. Double mutant stage 5-6 egg chambers with spn-A³ or mus301⁰⁹⁴ exhibit a two-oocyte phenotype: the second pro-oocyte develops as an oocyte rather than a nurse cell. The double mutants delay but do not block the decision between the two pro-oocytes as one of the two cells always becomes a nurse cell eventually.

(Gonzalez-Reyes et al., 1997)

Heterozygotes with Df(3R)sbd105 (spn-E^-) exhibit mislocalised oocyte phenotype and degenerating egg chambers.

(Gillespie and Berg, 1995)

maternal-effect lethal Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

spn-E¹/spn-E[+] is an enhancer of suppressor of variegation phenotype of Atf-2^c06407

(Seong et al., 2011)

NOT Enhancer of

Statement

Reference

spn-E¹/spn-E[+] is a non-enhancer of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

NOT Suppressor of

Statement

Reference

spn-E¹/spn-E[+] is a non-suppressor of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

spn-E¹/spn-E^hls-Δ125 has spermatocyte phenotype, enhanceable by Ste^unspecified

(Stapleton et al., 2001)

spn-E¹ has oocyte phenotype, enhanceable by spn-D²

(Gonzalez-Reyes et al., 1997)

NOT suppressed by

Statement

Reference

spn-E¹ has dorsal appendage phenotype, non-suppressible by mei-41^D3

(Klattenhoff et al., 2007)

Enhancer of

Statement

Reference

spn-E¹ is an enhancer of oocyte phenotype of spn-D²

(Gonzalez-Reyes et al., 1997)

Other

Statement

Reference

mus301⁰⁹⁴, spn-E¹ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

spn-A³, spn-E¹ has oocyte phenotype

(Gonzalez-Reyes et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The survival of roX1^ex33A Df(1)roX2Δ adult males is not significantly altered if they are also heterozygous for spn-E¹.

(Menon and Meller, 2012)

When Atf-2^c06407/+ is combined with spn-E¹/+, an additive effect on the derepression of w in In(1)w^m4 flies is observed.

(Seong et al., 2011)

The fused dorsal appendage phenotype of spn-E¹ eggs is not suppressed by mei-41^D3.

(Klattenhoff et al., 2007)

spn-E¹/+ does not impair cytotype-mediated repression of P-element excision.

(Simmons et al., 2007)

spn-E¹/ spn-E^hls-Δ125 primary spermatocytes have star-shaped crystals in combination with Ste^unspecified.

(Stapleton et al., 2001)

Double mutants with spn-D² increases the oocyte mispositioning frequency.

(Gonzalez-Reyes et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3327

ru¹ st¹ spn-E¹ e¹ ca¹/TM3, Sb¹ Ser¹

Kyoto

106980

Dp(1;Y)Bar^S; ru¹ st¹ spn-E¹ e¹ ca¹/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Strong mutation.

(Gonzalez-Reyes et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

fs(3)hls^E616

hls^E616

(Josse et al., 2007, Simmons et al., 2007, Grimaud et al., 2006, Pal-Bhadra et al., 2004, Stapleton et al., 2001, Gillespie and Berg, 1995)

hlsE616

(Kavi and Birchler, 2007)

spn-E¹

(Sokolova et al., 2023, Chen et al., 2021, Wang et al., 2020, Kotov et al., 2019, Teixeira et al., 2017, Andress et al., 2016, Barckmann et al., 2015, Menon and Meller, 2012, Seong et al., 2011, Shpiz et al., 2011, Rouget et al., 2010, Shpiz et al., 2009, Klattenhoff et al., 2007, Usakin et al., 2007, Kalmykova et al., 2005, Aravin et al., 2004)

spn-E⁶¹⁶

(Lin et al., 2023, Kordyukova et al., 2020, Stolyarenko, 2020, Ott et al., 2014, Patil et al., 2014, Kibanov et al., 2011, Pek and Kai, 2011, Pek and Kai, 2011, Patil and Kai, 2010, Lim et al., 2009, Lim and Kai, 2007, Lim and Kai, 2007, McCaffrey et al., 2006, Abdu et al., 2003, Findley et al., 2003, Kennerdell et al., 2002, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)

spnE¹

(Cui et al., 2021, Morgunova et al., 2021, Molla-Herman et al., 2015, Minakhina et al., 2014, Wei and Rong, 2007)

spnE⁶¹⁶

(Lim et al., 2022, Huynh and St. Johnston, 2000)

spnE^E616

(Anand and Kai, 2012, Snee and Macdonald, 2004)

Name Synonyms

Secondary FlyBase IDs

FBal0045709

References (57)

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