FB2025_01 , released February 20, 2025
Allele: Dmel\spn-E2
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General Information
Symbol
Dmel\spn-E2
Species
D. melanogaster
Name
FlyBase ID
FBal0016042
Feature type
allele
Associated gene
Dmel\spn-E
Associated Insertion(s)
Carried in Construct
Also Known As
spn-E653, hlsE653
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Gillespie and Berg, 1995)
Mutagen
ethyl methanesulfonate
(Gillespie and Berg, 1995, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Amino acid replacement: Y636term.

(Ott et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:15,841,351..15,841,351 [+]
Nucleotide change:

T15841351R

Amino acid change:

Y636term | spn-E-PA

Reported amino acid change:

Y636term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ott et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      dorsal appendage (with spn-Ehls-Δ125)

      (Ott et al., 2014)

      egg (with spn-Ehls-Δ125)

      (Ott et al., 2014)

      meiosis & nuclear chromosome

      (Stapleton et al., 2001)

      meiosis & nuclear chromosome (with spn-E1)

      (Stapleton et al., 2001)
      Detailed Description
      Statement
      Reference

      The eggs laid by spn-E2/spn-Ehls-Δ125 mutant females exhibit severe dorsoventral patterning defects, with a high percentage of collapsed eggs or eggs with no dorsal appendages.

      (Ott et al., 2014)

      spn-E2/spn-E1 larvae are not sensitive to MMS.

      (McCaffrey et al., 2006)

      spn-E1/spn-E2 animals show the same survival rate after exposure to 0.08% methyl methanesulfonate as control animals.

      (Abdu et al., 2003)

      Homozygotes and trans-heterozygotes with spn-E1 show an increased rate of non-disjunction in all except the small 4th chromosome. There are also substantial biases in recovery of reciprocal sperm classes. Nullo-XY sperm are recovered in considerable excess over the reciprocal XY sperm class. Also a weaker bias in favour of normal X-bearing sperm is seen.

      (Stapleton et al., 2001)

      Heterozygotes with Df(3R)sbd105 (spn-E-) exhibit mislocalised oocyte phenotype and degenerating egg chambers.

      (Gillespie and Berg, 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Weak mutation.

      (Gonzalez-Reyes et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      fs(3)hlsE653
      hlsE653
      (Stapleton et al., 2001, Gillespie and Berg, 1995)
      spn-E2
      spn-E653
      (Ott et al., 2014, McCaffrey et al., 2006, Abdu et al., 2003, Gonzalez-Reyes et al., 1997, Tearle and Nusslein-Volhard, 1987)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0045710
      References (7)