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General Information
Symbol
Dmel\stg4
Species
D. melanogaster
Name
FlyBase ID
FBal0016176
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
stg7M53, stg7M
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The number of cells in the epithelium of the hindgut at stage 13 is significantly reduced compared to wild type in stg4/Df(3R)BSC500 embryos.

stg4/Df(3R)BSC500 stage 13 embryos show defects in the left-right asymmetry in their hindgut, with some embryos showing no asymmetry, and other showing inverse asymmetry.

stg4 mutant embryos exhibit mitotic arrest in cell division cycle 14.

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

Eye of adults heterozygous with stghwy are normal, though missing macrochaetae phenotype remains.

Transheterozygotes with stgS114302b produce adult escapers that lack scutellars and many other bristles, have rough eyes and occasionally lack a haltere or have notched wings.

Lethal interaction with noose1 and noose2, and a semi-lethal interaction with noose3 and noose4. Transheterozygotes with stgknot are viable and fertile.

Primordial germ cells (PGCs) leave the posterior midgut (PMG) at the same time as in wild type and no significant differences in their migration to the gonadal mesoderm were observed.

Heterozygotes with DrMio and Dr1 are semi-lethal, 10-30% adults survive to eclosion, surviving adults exhibit reduced eye, deranged bristle pattern on the thorax, abdomen, legs and wing margins and locomotor defects so the flies could hardly move.

No cell division occurs in the ectoderm after the blastoderm stage in homozygotes. Cell death does not appear to occur in late embryos (stage 16) in contrast to wild-type.

The mesoderm is able to disperse and spread out normally after gastrulation in stg4 mutant embryos.

Mutants show a nonspecific reduction in the number of differentiated cuticular structures.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference

stg4 is a non-enhancer of visible phenotype of upd1GMR.PB

Suppressor of
Statement
Reference

stg4/stg[+] is a suppressor | partially of visible phenotype of mir-965KO1/mir-965KO2

wgSp-1, wg[+], stg4, stg[+] is a suppressor | partially of visible phenotype of mir-965KO1/mir-965KO2

stg4/stg[+] is a suppressor of increased cell death phenotype of shtd1

stg4/stg[+] is a suppressor of abnormal mitotic cell cycle phenotype of MybΔC.GMR

stg4/stg[+] is a suppressor of visible | dominant phenotype of MybΔC.GMR

NOT Suppressor of
Statement
Reference

stg4 is a non-suppressor of visible phenotype of upd1GMR.PB

Phenotype Manifest In
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

stg4 is a non-enhancer of eye phenotype of upd1GMR.PB

stg4 is a non-enhancer of phenotype of rux9

Suppressor of
Statement
Reference

stg4/stg[+] is a suppressor | partially of abdomen phenotype of mir-965KO1/mir-965KO2

wgSp-1, wg[+], stg4, stg[+] is a suppressor | partially of abdomen phenotype of mir-965KO1/mir-965KO2

stg4/stg[+] is a suppressor of eye phenotype of shtd1

stg4/stg[+] is a suppressor of ommatidium phenotype of shtd1

stg4/stg[+] is a suppressor of eye phenotype of MybΔC.GMR

NOT Suppressor of
Statement
Reference

stg4 is a non-suppressor of eye phenotype of upd1GMR.PB

stg4/stg4 is a non-suppressor of mesoderm phenotype of pbl3

stg4 is a non-suppressor of phenotype of rux9

Additional Comments
Genetic Interactions
Statement
Reference

One copy of stg4 partially suppresses the segment gap phenotype seen in the abdomens of mir-965KO1/mir-965KO2 mutant flies. The asynchronous histoblast division and reduction in histoblast migration rate are also suppressed.

One copy each of stg4 and wgSp-1 partially suppresses the segment gap phenotype seen in the abdomens of mir-965KO1/mir-965KO2 mutant flies.

Cell cycle arrested NB6-4t cells in CycEAR95;stg4 double mutants show glial identity.

Mesodermal cells in stumps09904b stg4 double mutant embryos are able to disperse from the site of mesoderm invagination.

In stg2/stg4,Df(3L)ri-79c double mutant embryos no post-blastoderm cell divisions occur and gastrulation is normal.

Lowering the dose of stg or cdc2 has no effect on the germline phenotype of rux.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Complements stghwy for ommatidial phenotype, fails to complement stghwy for bristle phenotype.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Trichogen and tormogen cells are absent in homozygous stg4 embryos, as assayed by Ecol\lacZA1-2-29 expression.

stg is not required for endoreplication of polytene DNA in the larva.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (44)