FlyBase Allele Report: Dmel\stnA[1]

General Information

Symbol

Dmel\stnA¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016201

Feature type

allele

Associated gene

Dmel\stnA

Associated Insertion(s)

Carried in Construct

Also Known As

stn^8P1, 8P1

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Petrovich et al., 1993)

Mutagen

X ray

(Perrimon et al., 1989, Schalet and Lefevre, 1973)

Progenitor genotype

Cytology

Description

The "stn^8P1" mutation disrupts stnA and stnB.

(Stimson et al., 1999)

Contains the InsOR20B 2.4kb insertion polymorphism present in Oregon-R but not Canton-S.

(Andrews et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | recessive

(Petrovich et al., 1993)

abnormal jumping

(Miklos et al., 1987)

abnormal locomotor behavior

(Miklos et al., 1987)

bang sensitive

(Miklos et al., 1987)

lethal

(Miklos et al., 1987)

partially lethal - majority die | recessive

(Petrovich et al., 1993)

uncoordinated

(Miklos et al., 1987)

Phenotype Manifest In

Detailed Description

Statement

Reference

Transheterozygotes between uncl¹⁰ and stnA¹ are viable and show no bang-sensitivity, paralysis or uncoordination at room temperature or after an hour at 29 degrees.

(Bloomington Drosophila Stock Center, 1998.9.23)

Semilethal as homozygotes. Flies heterozygous for stnA¹⁵ and any of the lethal stnA alleles show either a small or no off transient in ERGs. Flies heterozygous for a ts and a lethal allele of stnA show ERGs with off transient amplitudes between those for stnA⁷ and stnA¹⁵.

(Petrovich et al., 1993)

Homozygous viable. Homozygous germline clones display no maternal effect.

(Perrimon et al., 1989)

stnA¹/stnA⁶ flies emerge late, are bang sensitive, uncoordinated, have an abnormal jump response and become rapidly debilitated at 35^oC. stnA¹⁵/stnA¹ flies are ill, have no jump response and show uncoordinated behaviour after exposure to stress such as mechanical shock.

(Miklos et al., 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

stnA¹ has partially lethal - majority die | recessive phenotype, suppressible by Su(stn)¹

(Petrovich et al., 1993)

Other

Statement

Reference

Scer\GAL4^elav-C155, stnA¹, stnB¹, stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^shi.PS, stnA¹, stnA^AB.UAS, stnB¹, stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

stnA¹, stnB¹ has abnormal neuroanatomy phenotype

(Estes et al., 2003)

Scer\GAL4^elav-C155, stnA¹, stnA^AB.UAS, stnB¹, stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^shi.PS, stnA¹, stnB¹, stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^elav-C155, stnA¹, stnA^UAS.cEa, stnB¹ has lethal phenotype

(Estes et al., 2003)

stnA¹, stnB¹ has abnormal neurophysiology phenotype

(Estes et al., 2003)

stnA¹, stnB¹ has lethal phenotype

(Estes et al., 2003, Stimson et al., 2001)

stnA¹²/stnA¹, stnB¹/stnB¹² has lethal phenotype

(Stimson et al., 2001)

Phenotype Manifest In

Other

Statement

Reference

stnA¹, stnB¹ has bouton phenotype

(Estes et al., 2003)

stnA¹, stnB¹ has neuromuscular junction phenotype

(Estes et al., 2003, Stimson et al., 2001)

stnA¹, stnB¹ has neuromuscular junction & synaptic vesicle phenotype

(Stimson et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

stnA¹ stnB¹/Y ("stn^8P1") males do not survive to adulthood. stnA¹ stnB¹ third instar larvae show a reduced mean excitatory junction potential (EJP) amplitude at the neuromuscular junction compared to wild-type larvae. stnA¹ stnB¹ ("stn^8P1") larvae show dramatic alterations in presynaptic architecture at the neuromuscular junction; small bud-like "satellite" boutons are seen proliferating from normal boutons on the presynaptic motor terminals. The lethality of stnA¹ stnB¹/Y ("stn^8P1") animals is not rescued by expression of syt^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS.

(Estes et al., 2003)

Adult stnA¹ stnB¹ males survive at a frequency of approximately 1% relative to their control siblings. mejp frequency at the stnA¹ stnB¹ larval neuromuscular junction is nearly identical to that of controls. EJPs evoked by stimulation of the motor nerve are reduced to approximately 10% of wild type. This decreased EJP amplitude derives from a severe reduction in quantal content (4.7 +/- 0.7 compared to the wild-type 133.9 +/- 16.1). Average mejp amplitude and distribution of mejp amplitudes are not increased. The boutons of the stnA¹ stnB¹ larval neuromuscular junction show a 2.6-fold decrease in synaptic vesicle density compared to controls. The vesicles are on average 44.8 +/- 1.1 nm in diameter (compared to 34.4 +/- 0.6 nm for controls), corresponding to an approximately 2-fold increase in mean vesicle volume and in vesicle size variability.

(Stimson et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹ stnB¹/Y ("stn^8P1") animals. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹ stnB¹ ("stn^8P1") larvae. Expression of stnB^Scer\UAS.cEa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹ stnB¹/Y ("stn^8P1") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 rescues the mean excitatory junction potential (EJP) amplitude at the neuromuscular junction to wild-type in stnA¹ stnB¹ ("stn^8P1") larvae. Expression of stnA^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 does not rescue the lethality of stnA¹ stnB¹/Y ("stn^8P1") animals. The extra "satellite" bouton phenotype seen at the neuromuscular junction of stnA¹ stnB¹ ("stn^8P1") larvae is rescued by expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155. The extra "satellite" bouton phenotype seen at the neuromuscular junction of stnA¹ stnB¹ ("stn^8P1") larvae is rescued by expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^elav-C155.

(Estes et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4766

stnA¹/Dp(1;Y)y⁺mal¹⁰⁶/FM6

Notes on Origin

Discoverer

Associated with: stnB¹.

(Stimson et al., 2001)

Comments

The "stn^8P1" mutation affects both stnA and stnB protein levels; no stnA or stnB protein is detectable at the larval neuromuscular junction.

(Stimson et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

8P1

(Petrovich et al., 1993, Lefevre, 1981, Schalet and Lefevre, 1973)

l(1)8P1

(Munoz, 1992.12.16, Miklos et al., 1987)

stn¹

stn^8P1

(Estes et al., 2003, Stimson et al., 2001, Stimson et al., 1999, Andrews et al., 1996, Perrimon et al., 1989)

stnA¹

Name Synonyms

Secondary FlyBase IDs

References (13)

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