FlyBase Allele Report: Dmel\stnA[6]

General Information

Symbol

Dmel\stnA⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0016206

Feature type

allele

Associated gene

Dmel\stnA

Associated Insertion(s)

Carried in Construct

Also Known As

stn^ts1, stn^ts

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Petrovich et al., 1993, Miklos et al., 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: A104T.

(Wall et al., 2005)

This mutation (nucleotide substitution A104T) creates an ectopic AUG at +103 bp.

(Wall et al., 2005)

Nucleotide substitution: A104T. Amino acid replacement: K35M.

(Phillips et al., 2000)

Lesion may map to the stnA rather than the stnB transcription unit.

(Kelly, 1997.1.23)

Contains the InsOR20B 2.4kb insertion polymorphism present in Oregon-R but not Canton-S.

(Andrews et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:22,995,656..22,995,656 [-]

Nucleotide change:

A22995656T

Reported nucleotide change:

A104T

Amino acid change:

K35M | stnA-PC; K35M | stnA-PD; K35M | stnA-PE; K35M | stnA-PF

Reported amino acid change:

K35M

(Phillips et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

indirect flight muscle cell

(Kelly, 1983)

Detailed Description

Statement

Reference

At 22^oC flies are quite active though uncoordinated compared to wild type. At 29^oC flies cannot climb and walk in a stilted manner. Heterozygotes with Df(1)30A emerge late, become mired in the medium, and show low relative viability. Flies heterozygous for a ts and a lethal allele of stnA show ERGs with off transient amplitudes between those for stnA⁷ and stnA¹⁵. Flies heterozygous for stnA¹⁵ and a ts allele show ERG off transients significantly lower than for the control class, and lower than for the heterozygotes between ts and lethal alleles of stnA.

(Petrovich et al., 1993)

Uncoordinated leg and wing movement at 29^oC, restrictive temperature. Unusual jump response to a light-off stimulus.

(Perrimon et al., 1989)

Unusual jumping reaction in response to a light off stimulus and unable to coordinate movements at the restrictive temperature.

(Kelly et al., 1987)

Flies walk rather carefully and occasionally fall over at the permissive temperature of 22^oC. At 29^oC, the flies collapse to the bottom of their container and kick while on their backs. If they are then placed at 22^oC they recover. Hemizygotes with Df(1)JC4, Df(1)EA113, Df(1)JA27 or Df(1)HF359 have greatly reduced viability at the permissive temperature. They generally emerge late from the pupal case, rapidly become mired in the food medium and subsequently die. stnA¹⁵/stnA⁶ flies are very sedentary, are bang sensitive, uncoordinated and show marked debilitation at 35^oC. stnA⁶, sol¹ double heterozygotes have a normal optomotor response and normal viability.

(Miklos et al., 1987)

Severe behavioral debilitation is quickly induced by shift of adult stnA⁶ from 25^oC to 29^oC; abnormalities at high temperature include uncoordinated leg and wing movements, with complete paralysis never occurring (e.g., legs still move); stnA⁶ causes some debilitation at 22^oC (slow movements, occasionally falling over). stnA⁶ causes unusual jump response to light-off stimulus, which is more pronounced in combination with w; associated with this abnormal jumping is an increase in amplitude of light-off transient spike of electroretinogram (ERG); in tests of light-adapted stnA⁶, the jump response, as monitored by recordings from indirect flight muscles, habituates with increasing frequencies of light-off stimulation.

(Kelly, 1983)

temperature-sensitive behavioral mutant

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

stnB⁶/stnA⁶ is an enhancer of paralytic | heat sensitive phenotype of shi⁴

(Stimson et al., 2001)

stnB⁶/stnA⁶ is an enhancer of paralytic | heat sensitive phenotype of shi²

(Stimson et al., 2001)

NOT Enhancer of

Statement

Reference

stnB⁶/stnA⁶ is a non-enhancer of paralytic | heat sensitive phenotype of comt⁶

(Stimson et al., 2001)

stnB⁶/stnA⁶ is a non-enhancer of paralytic | heat sensitive phenotype of para^ts1

(Stimson et al., 2001)

Other

Statement

Reference

Dap160^EP2543, stnA⁶ has viable phenotype

(Kelly and Phillips, 2005)

Dap160^EP2543/Df(2L)TW65, stnA⁶ has viable phenotype

(Kelly and Phillips, 2005)

Dap160^EP2543/Df(2L)TW65, stnA⁶ has uncoordinated | adult stage phenotype

(Kelly and Phillips, 2005)

stnA⁶, stnB⁶ has decreased rate of movement phenotype

(Stimson et al., 2001)

Syt1^D27/Syt1^AD4, Syt1^elav.PD, stnA⁶ has lethal phenotype

(Phillips et al., 2000)

Syt1^D27/Syt1^AD4, Syt1^elav.PD, stnA⁶ has uncoordinated phenotype

(Phillips et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

stnB⁶/stnA⁶ is an enhancer of neuromuscular junction phenotype of shi²

(Stimson et al., 2001)

NOT Enhancer of

Statement

Reference

stnA¹², stnB⁶, stnA⁶, stnB¹² is a non-enhancer of neuromuscular junction phenotype of shi²

(Stimson et al., 2001)

Other

Statement

Reference

stnA⁶, stnB⁶ has neuromuscular junction & synaptic vesicle phenotype

(Stimson et al., 2001)

stnA¹²/stnA⁶, stnB⁶/stnB¹² has neuromuscular junction & synaptic vesicle phenotype

(Stimson et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

stnA⁶/Y ; Dap160^EP2543/Df(2L)TW65 males are viable but are somewhat late to eclose and are extremely uncoordinated as adults, dying within 48 hours of eclosion.

(Kelly and Phillips, 2005)

stnA⁶ stnB⁶ mutants show no decrease in synaptic vesicle density and no change in synaptic vesicle size at the larval neuromuscular junction (NMJ). Synaptic vesicle internalisation at the NMJ is delayed relative to wild type in stnA⁶ stnB⁶ mutants. The delay is even stronger in stnA⁶/stnA¹² stnB⁶/stnB¹² mutants. stnA⁶ stnB⁶ mutants are sluggish but do not show temperature-sensitive paralytic behaviour. At 28^oC, stnA⁶ stnB⁶ mutant NMJs show only a marginal increase in the rate of synaptic depression as compared with wild type. The effect on depression remains slight even at 30^oC. shi² stnA⁶ stnB⁶ flies undergo complete paralysis in 2 minutes at 26^oC. shi⁴ stnA⁶ stnB⁶ flies undergo complete paralysis in 2 minutes at 26^oC. At 28^oC, stnA⁶ stnB⁶ causes a marked enhancement of the weak neuromuscular junction depression seen in shi² single mutants. At 30^oC, there is no detectable effect of stnA⁶ stnB⁶ on depression.

(Stimson et al., 2001)

The viability of syt^D27/syt^AD4 flies (rescued to adulthood by syt^elav.PD) is significantly reduced in combination with stnA⁶/Y. The few surviving males have an extreme behavioural phenotype and are very sedentary and severely uncoordinated (they are more uncoordinated than stnA⁺/Y ; syt^D27/syt^AD4 flies rescued to adulthood by syt^elav.PD). They die within 48 hours of eclosion. syt^D27/syt^AD4 females (rescued to adulthood by syt^elav.PD) and also heterozygous for stnA⁶ show abnormal behaviour, including a spread leg "spider-like" appearance and a slightly uncoordinated gait.

(Phillips et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Putative cluster-mate of stnA⁷; both stnA⁶ and stnA⁷ were isolated from the same strain and have the same nucleotide substitution.

(Phillips et al., 2000)

Associated with: stnB⁶.

(Stimson et al., 2001)

Comments

The "stn^ts" mutation affects the levels of both stnA and stnB proteins at the larval neuromuscular junction; the mutation does not alter presynaptic levels of stnA protein consistently, although reduced levels are frequently seen, and a marked reduction in stnB protein levels is seen.

(Stimson et al., 2001)

Phosphorylation of two proteins 'IV' and 'D' from synaptosomal fractions of mutant flies is enhanced compared to wild-type.

(Kelly, 1983)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

stn⁶

stn^ts1

(Phillips et al., 2000, Kelly, 1997.1.23, Andrews et al., 1996, Petrovich et al., 1993, Perrimon et al., 1989, Kelly et al., 1987, Miklos et al., 1987, Miklos et al., 1987, Kelly, 1983)

stn^ts

(Kelly and Phillips, 2005, Wall et al., 2005, Stimson et al., 2001, Phillips et al., 1997)

stnA⁶

Name Synonyms

Secondary FlyBase IDs

References (15)

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