Homozygotes are phenotypically wild type at 18^oC.

After 3 days at the restrictive temperature of 29^oC, follicles do not develop beyond stage 9. The follicle cells gradually degenerate and there is a breakdown in intrafollicular polarity. Cytoplasmic extrusions, consisting either of ooplasm or nurse cell cytoplasm, are seen filling the space between two follicles and the epithelial sheath surrounding the ovariole. Cytoplasmic islands surrounded by membrane and consisting of abnormal cytoplasm containing condensed material or high concentrations of lipids are seen. Clusters of mitochondria and multilamellar bodies are present, and many follicle cell nuclei contain virus-like particles. Finally, follicles devoid of follicle cells fuse to form a tube. The morphology of the 16 cystocytes and mesodermal profollicle cells in the germarium of flies maintained at 29^oC for 7 days appears normal.

A cell-autonomous, temperature-sensitive recessive allele. Phenotype similar to that of su(f)¹² except that trypan-blue staining provides no evidence of cell death resulting from heat shock at stages of development in which such treatment induces leg duplications. Authors postulate that su(f)¹² discs developmentally impaired by heat shock, giving rise to observed abnormalities. Temperature-sensitive period from late second instar until two hours into pupariation. Shifts from 22^oC to 29^oC during the first larval instar leads to inability to pupariate; shifts between 112 and 164 h arrests adult development; shifts within the first six hours after the temperature for lethality removes bristles from the tergites. Gynandromorphs and somatic clones formed normally at permissive temperatures, but are not observed in adults produced at 29^oC. Ecdysteroid level of larvae raised at 29^oC are less than one-tenth that of wild type; pupariation can be induced by ecdysterone supplementation (Klose, Gateff, Emmerich and Beikirch, 1980). temperature-sensitive lethal