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General Information
Symbol
Dmel\Su(H)8
Species
D. melanogaster
Name
FlyBase ID
FBal0016309
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Su(H)SF8, SF8, l(2)br7SF8
Nature of the Allele
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

embryonic/first instar larval cuticle & embryonic head | germ-line clone

eye disc & neuron | somatic clone | cell autonomous

proneural cluster & dorsal mesothoracic disc

Detailed Description
Statement
Reference

When expression is driven using Scer\GAL4da.PU, Su(H)Scer\UAS.T:Avic\GFP,T:SV5\V5 rescues viability of Su(H)2/Su(H)8, although adult flies have some wing and eye defects (reminiscent of reduced N function).

Homozygotes are lethal during pupal development.

Heterozygous flies carrying out 10 training sessions to test memory of odours display a lower 24 hour memory in spaced training (rest periods between tasks) compared to control flies.

No difference in 24 hour memory is seen in heterozygotes carrying out massed training (no rest period) compared to controls.

Heterozygotes tested immediately and 3 hours after one training session did not show any difference in memory from wild type.

No unusual cell death is observed in wing pouch clones.

Mitotic clones of cells carrying Su(H)8 grow normally at any position within the eye disc.

Su(H)8/Su(H)6 late third instar larvae are almost totally devoid of crystal cells, but have wild-type plasmatocyte cell counts. These larvae also have increased numbers of prophenoloxidase expressing cells in the larval lymph glands.

Homozygous follicle cell clones are smaller than surrounding wild-type cells and the mutant cells undergo additional mitoses.

Wing primordium is established but does not grow. Lateral inhibition fails in the notum, causing an excess of neural precursors.

In Su(H)8/Su(H)2 mutant wing discs, each proneural cluster is enlarged as compared to wild-type.

Homozygous clones do not reliably disrupt the dorsal/ventral boundary in the wing disc.

The number of ftz expressing MP2 neurons increases compared to wild-type (About 15 on each side of the midline, as compared to 2 in wild-type) in homozygous embryos derived from homozygous female germ-line clones (lacking both maternal and zygotic function). In homozygous embryos derived from homozygous female germ-line clones (lacking both maternal and zygotic function), RP2sib neurons become eve expressing RP2 neurons. Usib neurons also appear to become U neurons.

Mutant embryos develop a larger patch of dorsal cuticle than that developed by corresponding N mutants.

Many cells in clones induced in the central domain of the ZNC in the developing wing enter S phase. Those in anterior clones located next to wg expressing cells arrest in G2.

Su(H)2/Su(H)8 exhibit loss of wing blade material.

Homozygous clones in the eye imaginal disc show autonomous neural hypertrophy. Many of these ectopic neural cells are R8 photoreceptors.

The number of cells in the nau-expressing muscle precursor clusters is wild-type in homozygous embryos. The number of cells in the nau-expressing muscle precursor clusters is increased compared to wild-type in homozygous embryos derived from homozygous female germline clones (lacking both maternal and zygotic function). The number of cells in the nau-expressing muscle precursor clusters is increased compared to wild-type in homozygous DlM2 embryos. The severity of the phenotype is not altered if the embryos are also homozygous for Su(H)8.

Mutation suppresses the hair cell to socket cell transformation, but not the neuron to sheath cell transformation. Homozygous clones in the IIa cell due to Scer\FLP1Scer\UAS.cBa expression driven by Scer\GAL4sca-537.4 or Scer\GAL4sca-109-68 cause a twinned hair phenotype.

Su(H)8/Su(H)IB115 wings are reduced to stumps.

Homozygous embryos derived from germ-line clones exhibit a strong neurogenic cuticular phenotype and fail to hatch. The number of neuroblasts and sensory precursors that segregate from the ventral and dorsolateral neuroectoderm, respectively, is greatly increased. This leads to hypertrophy of the CNS and PNS and failure of ventral and head cuticle to develop. No defect is seen in the nervous system when rescued by wild type paternal copy of Su(H). Heterozygous embryos derived from germ-line clones survive to adulthood.

Cells within homozygous clones located at wild type sensory organ positions adopt the sensory organ precursor (SOP) fate, even when directly juxtaposed to wild type cells. Su(H) mutant SOPs express a neuronal fate.

Mosaic analysis reveals two bristle phenotypes. When recombination is induced in the first and second larval instar patches of naked cuticle result. Recombination later results in double-shaft bristles.

Lethality during the first day of pupal development. Supernumerary cells stained in mutant imaginal discs have adopted a sensory organ precursor (SOP) fate instead of the epidermal fate they would normally express. The excess SOPs arise from the proneural clusters of potential precursor cels that appear in the imaginal discs during the development of wild type bristles. The wing pouch region of the wing imaginal disc and the retinal field of the eye-antennal imaginal disc are dramatically reduced. Identical phenotype is seen when transheterozygous with Df(2L)TE35BC-24, Su(H)1, Su(H)2 or Su(H)IB115.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Su(H)8/Su(H)[+] is an enhancer of visible | adult stage phenotype of Scer\GAL4sca-109-68, hamUAS.cMa

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Su(H)8 is a suppressor of visible phenotype of numbSW

Su(H)8 is a suppressor of visible phenotype of numb2

NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

Su(H)8/Su(H)[+] is a non-enhancer of wing phenotype of pydtam/pydex147

Su(H)8 is a non-enhancer of wing phenotype of Scer\GAL4bbg-C96, mamN.UAS

Su(H)8 is a non-enhancer of phenotype of Nspl-1

Su(H)8 is a non-enhancer of phenotype of Nnd-1

Suppressor of
Statement
Reference

Su(H)8 is a suppressor | partially of adult head phenotype of Tom8

Su(H)8/Su(H)[+] is a suppressor of phenotype of Src42ASu(Raf)1-1

Su(H)8 is a suppressor of trichogen cell phenotype of numbSW

Su(H)8 is a suppressor of trichogen cell phenotype of numb2

Su(H)8 is a suppressor of phenotype of H1

Su(H)8 is a suppressor of phenotype of H2

NOT Suppressor of
Statement
Reference

Su(H)8/Su(H)del47 is a non-suppressor of wing disc phenotype of aprK568/apUGO35

Su(H)8 is a non-suppressor of phenotype of Nspl-1

Other
Additional Comments
Genetic Interactions
Statement
Reference

Wings from pydex147/pydtam flies are broader compared to control wings, and this phenotype is not modified in a Su(H)8/+ background.

Su(H)2 Sox154AA/Su(H)8 Sox154AA; Su(H)RC-ΔASE mutants show a more dramatic socket differentiation defect than either single mutant. Most of the double mutant bristles have a convex (rather than concave) socket-shaft cuticular interface. Dome of these bulbous socket structures extend one or more small cuticular projections, while others extend one or two distinctly shaft-like structures.

Wing pouch clones double mutant for l(2)gd1d7 and Su(H)8 show the same increase in cell death as observed in l(2)gd1d7 clones.

apUGO35/aprK568; HE31 and apUGO35/aprK568; Su(H)del47/Su(H)8 wing discs fail to form a dorsal/ventral compartment boundary.

The neurogenic phenotype, but not the wing development phenotype, of Su(H)2/Su(H)8 is strongly suppressed by either HLHm7Scer\UAS.cdCa or E(spl)Scer\UAS.cNa driven by Scer\GAL4klu-G410. The wing development phenotype of Su(H)2/Su(H)8 is not suppressed by either Nint.SH.Scer\UAS or DlScer\UAS.cHa, or SerScer\UAS.cSa in combination with vgScer\UAS.cKa, driven by Scer\GAL4dpp.blk1. The wing development phenotype of Su(H)2/Su(H)8 is also not suppressed by HE31.

Partially suppresses the Tom8 phenotype - Su(H)8/+ ; Tom8/Tom8 pharate adults show almost completely everted head structures. The larval lethality and small eye-antennal disc phenotype of Su(H)8 homozygotes can be partially rescued by Tom8 - Su(H)8/Su(H)8 ; Tom8/+ animals develop to the pupal stage with pharate adults showing partially everted heads.

The addition of Su(H)2/Su(H)8 has no effect on the proneural cluster phenotype in the wing disc seen in NECNΔ10-12.Scer\UAS/Scer\GAL4ptc-559.1 discs. Expression of Ncdc10.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4ptc-559.1 has no effect on the Su(H)8/Su(H)2 proneural cluster phenotype in the wing disc.

Does not enhance the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

Dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

Scer\GAL4dpp.blk1-mediated or Scer\GAL4ptc-559.1-mediated expression of vgScer\UAS.cKa substantially rescues the wing blade phenotype. Scer\GAL4dpp.blk1-mediated expression of wgScer\UAS.cGa does not rescue loss of wing tissue.

Double mutant clones (numb2 Su(H)8) exhibit the phenotype caused due to loss of function of Su(H) alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Not rescued by
Comments

When expression is driven using Scer\GAL4da.PU, Su(H)Scer\UAS.T:Avic\GFP,T:SV5\V5 rescues viability of Su(H)2/Su(H)8.

Expression of Su(H)hs.PS with 30 minutes heat shock at 37C rescues the reduced 24 hour memory phenotype seen in Su(H)8 homozygotes.

When expression of Su(H)Scer\UAS.cKa is driven by Scer\GAL4dpp.blk1 in a Su(H)2/Su(H)8 mutant the wing pouch size defect is rescued. When expression of Su(H)Scer\UAS.cKa is driven by Scer\GAL4klu-G410 in a Su(H)2/Su(H)8 mutant the neurogenesis and wing pouch size defects are rescued.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Ashburner.

Comments
Comments

Double mutant analysis fails to establish a strict epistatic relationship between H and Su(H) for the specification of sensory organ precursor fate.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (74)